EGLN1/PHD2 Antibody [HRP]

Images

 
There are currently no images for EGLN1/PHD2 Antibody (NB100-137H).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, RbSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP

EGLN1/PHD2 Antibody [HRP] Summary

Immunogen
The epitope recognized by this antibody maps to a region between residues 1 and 50 of human PHD2/HIF Prolyl Hydroxylase 2 using the numbering given in entry NP_071334.1 (GeneID 54583).
Localization
Cytoplasm, nucleus
Predicted Species
Rabbit (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EGLN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This PHD2 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Western Blot, and Immunohistochemistry-paraffin embedded sections. In ICC/IF, cytoplamic and nuclear staining was observed in HeLa cells. Immunoprecipitation was reported in scientific literature.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System

Reactivity Notes

Results have been mixed in Rat with success in western analysis and immunofluorescence on Rat endothelial cells and negative results with PC12 cells. Expected reactivity with Rabbit, Orangutan, Rhesus Monkey, Gorilla based on 100% sequence identity.Mouse reactivity reported in scientific literature (PMID: 25578858) Rat reactivity reported in scientific literature (PMID: 25635047). Primate reactivity reported in scientific literature (PMID: 25974097)

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for EGLN1/PHD2 Antibody [HRP]

  • C1orf12
  • C1orf12HPH2
  • EC 1.14.11
  • EC 1.14.11.-
  • ECYT3
  • egl nine homolog 1 (C. elegans)
  • egl nine homolog 1
  • egl nine-like protein 1
  • EGLN1
  • HIF prolyl hydroxylase 2
  • HIFPH2 EGL nine (C.elegans) homolog 1
  • HIFPH2
  • HIF-PH2
  • HIF-prolyl hydroxylase 2
  • HPH2
  • HPH-2
  • Hypoxia-inducible factor prolyl hydroxylase 2
  • PHD2
  • PHD2SM20
  • Prolyl hydroxylase domain-containing protein 2
  • SM20
  • SM-20
  • zinc finger MYND domain-containing protein 6
  • ZMYND6
  • ZMYND6DKFZp761F179

Background

HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degredation via the von Hippel-Lindau ubiquitylation complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-105
Species: Hu, Mu, Rt, Po, Bv, Ft, Mk, Pm, Rb, Sh, Xp, Ye
Applications: WB, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, TCS, KO, LA
NB100-139
Species: Hu, Mu, Rt
Applications: WB, EM, ICC/IF, IHC, IHC-P, IP
AF6394
Species: Hu
Applications: WB, IP
NB100-485
Species: Hu
Applications: WB, IP
NB100-122
Species: Hu, Mu, Rt, Fi, Ha, Pm, Sh
Applications: WB, Simple Western, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO
NBP1-89843
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-428
Species: Hu, Mu, Rt
Applications: WB, EM, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB120-19347
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IF
NB100-124
Species: Hu, Mu, Rt, Bv, Ft, Pm, Sh
Applications: WB, ChIP, GS, ICC/IF, IHC, IHC-P, IP
NBP2-32964
Species: Hu
Applications: Flow, ICC/IF, IHC-Fr
NB100-417
Species: Hu, Mu, Rt, Ca, Pl
Applications: WB, Simple Western, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-89150
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-21037
Species: Hu
Applications: WB, IHC, IHC-P
H00011026-M01
Species: Hu
Applications: WB, ELISA
NBP1-31302
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-137H
Species: Hu, Mu, Rt, Pm, Rb
Applications: WB, IHC, IHC-P

Publications for EGLN1/PHD2 Antibody (NB100-137H) (0)

There are no publications for EGLN1/PHD2 Antibody (NB100-137H).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for EGLN1/PHD2 Antibody (NB100-137H) (0)

There are no reviews for EGLN1/PHD2 Antibody (NB100-137H). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EGLN1/PHD2 Antibody (NB100-137H) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional EGLN1/PHD2 Products

Bioinformatics Tool for EGLN1/PHD2 Antibody (NB100-137H)

Discover related pathways, diseases and genes to EGLN1/PHD2 Antibody (NB100-137H). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for EGLN1/PHD2 Antibody (NB100-137H)

Discover more about diseases related to EGLN1/PHD2 Antibody (NB100-137H).
 

Pathways for EGLN1/PHD2 Antibody (NB100-137H)

View related products by pathway.

PTMs for EGLN1/PHD2 Antibody (NB100-137H)

Learn more about PTMs related to EGLN1/PHD2 Antibody (NB100-137H).
 

Research Areas for EGLN1/PHD2 Antibody (NB100-137H)

Find related products by research area.

Blogs on EGLN1/PHD2

There are no specific blogs for EGLN1/PHD2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our EGLN1/PHD2 Antibody [HRP] and receive a gift card or discount.

Bioinformatics

Gene Symbol EGLN1
Entrez