EGLN1/PHD2 Antibody

Images

 
Immunohistochemistry: EGLN1/PHD2 Antibody [NB100-2219] - Immunofluorescence of wounded tissue and normal unwounded skin. Representative images for immunofluorescent staining of PHD-2 on healed wounded skin and adjacent ...read more
Genetic Strategies: Knockdown Validated: EGLN1/PHD2 Antibody [NB100-2219] - In vitro analysis of PHD-2 knockout and protein quantification.Western blot data for PHD-2 knockout in fibroblasts of heterozygous Col1 ...read more
Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody [NB100-2219] - Analysis of a FFPE mouse lung section using 1:200 dilution of EGLN1/PHD2 antibody. The staining was developed using HRP conjugated anti-rabbit secondary ...read more
Western Blot: EGLN1/PHD2 Antibody [NB100-2219] - Detection of EGLN1/PHD2 in mouse kidney lysate. ECL exposure, 20 seconds.
Immunocytochemistry/ Immunofluorescence: EGLN1/PHD2 Antibody [NB100-2219] - EGLN1/PHD2 antibody at 1:500 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight ...read more
Immunocytochemistry/ Immunofluorescence: EGLN1/PHD2 Antibody [NB100-2219] - Analysis of EGLN1/PHD2 in ARPE-19 cells using anti-PHD2 antibody. Image from veriifed customer review.
Immunohistochemistry: EGLN1/PHD2 Antibody [NB100-2219] - Staining of renal tubular epithelium in mouse using NB100-2219 at 2.5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

EGLN1/PHD2 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of mouse PHD2/HIF Prolyl Hydroxylase 2 (between residues 300-400). [Uniprot: Q91YE3]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EGLN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 2.5 - 5.0 ug/mL
  • Immunohistochemistry-Paraffin 2.5 - 5.0 ug/ml
  • Immunoprecipitation 1:10 - 1:500
  • Knockdown Validated
  • Knockout Validated
Application Notes
In Western blot a band is seen ~43 kDa representing HIF Prolyl Hydroxylase 2. There is also a non-specific band of similar intensity at ~75 kDa. Knockout Validation was reported in scientific literature (PMID: 24695462).
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Mouse Kidney Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
EGLN1/PHD2 Peptide (NB100-2219PEP)
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB100-2219 in the following applications:

Publications
Read Publications using
NB100-2219 in the following applications:

  • 1 publication
  • IP
    1 publication
  • KO
    1 publication
  • WB
    19 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EGLN1/PHD2 Antibody

  • C1orf12
  • C1orf12HPH2
  • EC 1.14.11
  • EC 1.14.11.-
  • ECYT3
  • egl nine homolog 1 (C. elegans)
  • egl nine homolog 1
  • egl nine-like protein 1
  • EGLN1
  • HIF prolyl hydroxylase 2
  • HIFPH2 EGL nine (C.elegans) homolog 1
  • HIFPH2
  • HIF-PH2
  • HIF-prolyl hydroxylase 2
  • HPH2
  • HPH-2
  • Hypoxia-inducible factor prolyl hydroxylase 2
  • PHD2
  • PHD2SM20
  • Prolyl hydroxylase domain-containing protein 2
  • SM20
  • SM-20
  • zinc finger MYND domain-containing protein 6
  • ZMYND6
  • ZMYND6DKFZp761F179

Background

HIF prolyl 4-hydroxylases (PHDs) are proyl hydroxylase domain-containing enzymes (PHD1/ Egln2, PHD2/ Egln1, PHD3/ Egln3, and P4H-TM) that mediate important physiological responses to hypoxia by modulating HIF1alpha levels. The HIF-alpha is regulated by hydroxylation, both by a family of PHDs leading to ubiquitination and proteasomal degradation, and by transcriptional inactivation following asparaginyl hydroxylation by FIH (factor inhibiting HIF). Under normoxic conditions, HIF Prolyl Hydroxylase 2 (HIF1-PH; also called PHD1 or Egln2) catalyzes the post-translational formation of 4-hydroxyproline through hydroxylation of a specific proline found in each of NODD/CODD domains of HIF1A and also hydroxylates HIF2A with preference for CODD site of HIF1A/HIF1B. After hydroxylation, HIFs undergo proteasomal degradation via VHL (von Hippel-Lindau) ubiquitination complex. However, under hypoxic conditions, the hydroxylation reaction is tempered which allows HIFs to escape degradation process followed by their nuclear translocation, heterodimerization with HIF1B, and increased expression of hypoxia-inducible genes. HIF1-PH, through regulating HIF1 stability, is involved in various hypoxia-influenced processes such as angiogenesis in retinal/cardiac functionality as well as tumor angiogenesis, and defecticve EGLN1 have been linked to ECYT3 (familial erythrocytosis type 3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EGLN1/PHD2 Antibody (NB100-2219)(32)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: IHC-P, IP, KO, WB.


Filter By Application
IHC-P
(1)
IP
(1)
KO
(1)
WB
(19)
All Applications
Filter By Species
Human
(5)
Mouse
(15)
Rat
(4)
All Species
Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using NB100-2219 Applications Species
Laitakari A, Huttunen R, Kuvaja P et al. Systemic long-term inactivation of hypoxia-inducible factor prolyl 4-hydroxylase 2 ameliorates aging-induced changes in mice without affecting their life span FASEB J. Feb 25 2020 [PMID: 32100354] (WB, Mouse) WB Mouse
Kim S, Jo CH, Kim GH Effects of empagliflozin on nondiabetic salt-sensitive hypertension in uninephrectomized rats Hypertens. Res. Sep 19 2019 [PMID: 31537914] (WB, Rat) WB Rat
Chowdhury D, Alrefai H, Landero Figueroa JA et al. Metallothionein 3 Controls the Phenotype and Metabolic Programming of Alternatively Activated Macrophages Cell Rep Jun 25 2019 [PMID: 31242420] (WB, Mouse) WB Mouse
Yamamoto A, Hester J, Macklin PS, et al. Systemic silencing of PHD2 causes reversible immune regulatory dysfunction J. Clin. Invest. 2019 Jun 04 [PMID: 31162141] (WB, Mouse) WB Mouse
Sim J, Cowburn AS, Palazon A et al. The Factor Inhibiting HIF Asparaginyl Hydroxylase Regulates Oxidative Metabolism and Accelerates Metabolic Adaptation to Hypoxia. Cell Metab. Apr 3 2018 [PMID: 29617647] (WB, Mouse) WB Mouse
Akat F, Ficicilar H, Durak A et al. Intermittent hypoxia induces beneficial cardiovascular remodeling in left ventricular function of type 1 diabetic rat. Anatol J Cardiol 2018 Apr [PMID: 29615543] (Rat) Rat
Flisinski M, Wisniewska-Chudy E, Brymora A et al. Chronic kidney disease leads to hypoxia inducible factor-1alpha to hypoxia inducible factor-2alpha switch in the gastrocnemius muscle J. Physiol. Pharmacol. 2017 Jun 01 [PMID: 28820398] (WB, Rat) WB Rat
Wang S, Zeng H, Chen ST et al. Ablation of endothelial prolyl hydroxylase domain protein-2 promotes renal vascular remodelling and fibrosis in mice. J. Cell. Mol. Med. Mar 7 2017 [PMID: 28266128] (WB, Mouse) WB Mouse
Choi JE, Kim JH, Song NY et al. 15-Deoxy-delta12, 14-prostaglandin J2 stabilizes hypoxia inducible factor-1alpha through induction of heme oxygenase-1 and direct modification of prolyl-4-hydroxylase 2. Free Radic. Res. Sep 6 2016 [PMID: 27598034] (IP, Human) IP Human
Zeng H, He X, Tuo QH et al. LPS causes pericyte loss and microvascular dysfunction via disruption of Sirt3/angiopoietins/Tie-2 and HIF-2alpha/Notch3 pathways. Sci Rep. 2016 Feb 12 [PMID: 26868537] (WB, Human, Mouse) WB Human, Mouse
Show All 32 Publications.

Reviews for EGLN1/PHD2 Antibody (NB100-2219) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-2219:
Filter by Applications
WB
(2)
IF
(1)
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Filter by Species
Human
(1)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot EGLN1/PHD2 NB100-2219
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reviewed by:
Anonymous
WB Mouse 02/16/2017
View

Summary

ApplicationWestern Blot
Sample Testedmurine bone marrow derived macrophages
SpeciesMouse
Immunofluorescence EGLN1/PHD2 NB100-2219
Enlarge
reviewed by:
Helder Andre
IF Human 05/14/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedARPE-19 cells
SpeciesHuman

Blocking

Blocking Details10% goat serum in PBS-TritonX (0.1%) (30min @ RT)

Primary Anitbody

Dilution Ratio1:500 in blocking solution (1h @ RT)

Secondary Antibody

Secondary Description1:1000 Goat anti-rabbit IgG-A488 in blocking solution (1h @ RT)

Details

Detection NotesEpifluorescence microscope
Fixation DetailsFixed in 4% buffered formalin (15min @ RT); Permeabilized in PBS-TritonX (0.5%) (10min @ RT)
Wash Description3x5min PBS
 
reviewed by:
Anonymous
WB Mouse 01/18/2012
View

Summary

ApplicationWestern Blot
Sample Testednuclear extract (brain), Sample Amount: 20ug
SpeciesMouse

Blocking

Blocking DetailsBlocking Buffer: 10% TPBS, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:1000, Incubation Dilution Buffer: blocking buffer, Incubation Time: overnight, Incubation Temp: 4 C

Product General Protocols

View specific protocols for EGLN1/PHD2 Antibody (NB100-2219): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EGLN1/PHD2 Antibody (NB100-2219) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-2219AF350
Alexa Fluor 405 NB100-2219AF405
Alexa Fluor 488 NB100-2219AF488
Alexa Fluor 594 NB100-2219AF594
Alexa Fluor 647 NB100-2219AF647
Alexa Fluor 700 NB100-2219AF700
Alexa Fluor 750 NB100-2219AF750
Biotin NB100-2219B
DyLight 350 NB100-2219UV
DyLight 405 NB100-2219V
DyLight 488 NB100-2219G
DyLight 550 NB100-2219R
DyLight 594 NB100-2219DL594
DyLight 650 NB100-2219C
DyLight 680 NB100-2219FR
DyLight 755 NB100-2219IR
FITC NB100-2219F
HRP NB100-2219H
Janelia Fluor 549 NB100-2219JF549
Janelia Fluor 646 NB100-2219JF646

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Bioinformatics Tool for EGLN1/PHD2 Antibody (NB100-2219)

Discover related pathways, diseases and genes to EGLN1/PHD2 Antibody (NB100-2219). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Anonymous
02/16/2017
Application: WB
Species: Mouse

Helder Andre
05/14/2015
Application: IF
Species: Human

 
Anonymous
01/18/2012
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol EGLN1