IKK beta Antibody (10AG2) [Janelia Fluor® 646]

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IP, WB, CyTOF-ready
• Clone: 10AG2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 646

IKK beta Antibody (10AG2) [Janelia Fluor® 646] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: A full-length human IKKb recombinant protein was used to immunize mice (NP_001547).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IKBKB
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB100-56509. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34333329)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for IKK beta Antibody (10AG2) [Janelia Fluor® 646]

• EC 2.7.11
• EC 2.7.11.10
• I-kappa-B kinase 2
• I-kappa-B-kinase beta
• IkBKB
• IKK beta
• IKK2
• IKK2FLJ38368
• IKK-B
• IKK-betaFLJ36218
• IKKBFLJ40509
• inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• inhibitor of nuclear factor kappa-B kinase subunit beta
• MGC131801
• NFKBIKB
• NFKBIKBFLJ33771
• Nuclear factor NF-kappa-B inhibitor kinase beta

Background

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAP kinase kinase kinase-1 (MEKK1) (7), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli (9). Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo (9).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IKBKB