This CD11b/c antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot, Immunohistochemistry paraffin embedded sections and Flow Cytometry. In Western blot a specific band is observed ~ 160 kDa and an apparant non-specific band is observed ~ 60 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with citrate buffer (pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Raw 264.7 cells.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
160 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 4 using NB110-40766F in the following applications:
Rat reactivity reported in scientific literature (PMID: 25150592)
Packaging, Storage & Formulations
Store at 4C in the dark.
0.05% Sodium Azide
Immunogen affinity purified
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for CD11b/c Antibody [FITC]
antigen CD11b (p170)
CD11 antigen-like family member B
CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide)
Cell surface glycoprotein MAC-1 subunit alpha
CR-3 alpha chain
integrin, alpha M (complement component 3 receptor 3 subunit)
Leukocyte adhesion receptor MO1
macrophage antigen alpha polypeptide
neutrophil adherence receptor alpha-M subunit
Neutrophil adherence receptor
CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.