IKK alpha Antibody (14A231) - BSA Free

Images

 
Western Blot: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - IKK alpha Antibody (14A231) [NB100-56704] - Analysis using Azide Free version of NB100-56704. IKK in Daudi cell lysate using IKKa monoclonal antibody ...read more
Immunocytochemistry/ Immunofluorescence: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% ...read more
Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - IKK alpha Antibody (14A231) [NB100-56704] - Analysis using Azide Free version of NB100-56704. FFPE human kidney stained with IKKa ...read more
Flow Cytometry: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - IKK alpha Antibody (14A231) [NB100-56704] - Intracellular staining of HEK293 cells using 0.5 ug of IKK alpha antibody. Green histogram represents ...read more
Western Blot: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - IKK alpha Antibody (14A231) [NB100-56704] - Analysis of IKK alpha in Daudi cell lysate using IKK alpha antibody at 1 ug/mL.
Western Blot: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - IKK alpha Antibody (14A231) [NB100-56704] - Total protein from various Human, Mouse and Rat cell lines were separated on a 12% gel by SDS-PAGE, ...read more
Immunocytochemistry/ Immunofluorescence: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) ...read more
Immunocytochemistry/ Immunofluorescence: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% ...read more
Simple Western: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - IKK alpha Antibody (14A231) [NB100-56704] - Image shows a specific band for IKK alpha in 0.5 mg/mL of Daudi (left) and U937 (right) lysate. This ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
14A231
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

IKK alpha Antibody (14A231) - BSA Free Summary

Immunogen
This antibody was raised against a His-tagged full-length human IKK alpha protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CHUK
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Immunoprecipitation 1 - 2 ug/mL
  • Flow (Intracellular) 0.25 - 0.5 ug/10^6 cells
Application Notes
Use in Flow Intracellular reported in scientific literature (PMID 24804954). Use in IHC-Frozen reported in scientific literature (PMID 25133425). In Western blot a 85 kDa band is observed.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in chromatin immunoprecipitation reported in scientific literature (PMID 27705798). Use in Immunohistochemistry reported in scientific literature (PMID: 27121058).
Control
IKK alpha Knockout HeLa Cell Lysate
Publications
Read Publications using
NB100-56704 in the following applications:

Reactivity Notes

New World Monkey

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS - BSA free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for IKK alpha Antibody (14A231) - BSA Free

  • CHUK
  • conserved helix-loop-helix ubiquitous kinaseIKBKA
  • EC 2.7.11
  • EC 2.7.11.10
  • I-kappa-B kinase 1
  • I-kappa-B kinase alpha
  • IkappaB kinase
  • IkBKA
  • IkBKalpha
  • IKK alpha
  • IKK1
  • IKK1I-kappa-B kinase-alpha
  • IKK-A
  • IKKAIkB kinase alpha subunit
  • IKK-alphainhibitor of nuclear factor kappa-B kinase subunit alpha
  • NFKBIKA
  • NFKBIKAIKK-a kinase
  • Nuclear factor NFkappaB inhibitor kinase alpha
  • Nuclear factor NF-kappa-B inhibitor kinase alpha
  • TCF16
  • TCF-16
  • Transcription factor 16

Background

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. In vitro, IKK1/a and IKK2/b can form homo- and heterodimers that can phosphorylate IkBs at the regulatory serine residues directly. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Targeted disruption of IKK1/a gene in mice results in skin and limb abnormalities and death of newborns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for IKK alpha Antibody (NB100-56704)(77)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: ChIP, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ChIP
(1)
Flow-IC
(1)
ICC/IF
(1)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
IP
(4)
WB
(27)
All Applications
Filter By Species
Human
(15)
Mouse
(15)
All Species
Showing Publications 1 - 10 of 77. Show All 77 Publications.
Publications using NB100-56704 Applications Species
Liang S, Ma HY, Zhong Z et al. NADPH Oxidase 1 in Liver Macrophages Promotes Inflammation and Tumor Development in Mice Gastroenterology Mar 1 2019 [PMID: 30445007] (WB, Mouse) WB Mouse
Heringer JSP et al. The role of IKKalpha during diet-induced obesity and glucose intolerance in insulin sensitive tissues. The role of glycosylation during intestinal carcinogenesis Thesis. 2014 (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Balkhi MY, Willette-Brown J, Wittmann G, Hu Y. IKKa deficiency disrupts the development of marginal zone and follicular B cells Genes Immun. May 8 2018 [PMID: 29740197] (Mouse) Mouse
Todoric J, Antonucci L, Di Caro G et al. Stress-Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas Cancer Cell. 2017 Nov 07 [PMID: 29153842] (WB, Human) WB Human
Schwartz K, Bochkariov D. Novel chemiluminescent Western blot blocking and antibody incubation solution for enhanced antibody-antigen interaction and increased specificity Electrophoresis 2017 Jul 13 [PMID: 28704589] (WB) WB
Zhu F, Willette-Brown J, Song NY et al. Autoreactive T Cells and Chronic Fungal Infection Drive Esophageal Carcinogenesis. Cell Host Microbe. Apr 12 2017 [PMID: 28407484]
Diamanti MA, Gupta J, Bennecke M et al. IKKa controls ATG16L1 degradation to prevent ER stress during inflammation J. Exp. Med. Jan 12 2017 [PMID: 28082356] (WB) WB
Alameda JP, Navarro M, Ramirez A et al. IKKa regulates the stratification and differentiation of the epidermis: implications for skin cancer development. Oncotarget. Nov 22 2016 [PMID: 27732959] (IHC-P, WB, Human) IHC-P, WB Human
Shintaku J, Peterson JM, Talbert EE et al. MyoD Regulates Skeletal Muscle Oxidative Metabolism Cooperatively with Alternative NF-B. Cell reports Jul 8 1905 [PMID: 27705798] (ChIP, WB, Mouse) ChIP, WB Mouse
Alameda JP, Gaspar M, Ramirez A et al. Deciphering the role of nuclear and cytoplasmic IKKalpha in skin cancer. Oncotarget. May 17 2016 [PMID: 27121058] (IHC, Human) IHC Human
Show All 77 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for IKK alpha Antibody (NB100-56704) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-27409AF350
Alexa Fluor 405 NBP2-27409AF405
Alexa Fluor 488 NBP2-27409AF488
Alexa Fluor 532 NBP2-27409AF532
Alexa Fluor 594 NBP2-27409AF594
Alexa Fluor 647 NBP2-27409AF647
Alexa Fluor 700 NBP2-27409AF700
Alexa Fluor 750 NBP2-27409AF750
Allophycocyanin NBP2-27409APC
Biotin NBP2-27409B
DyLight 350 NBP2-27409UV
DyLight 405 NBP2-27409V
DyLight 488 NBP2-27409G
DyLight 550 NBP2-27409R
DyLight 594 NB100-56704DL594
DyLight 650 NBP2-27409C
DyLight 680 NBP2-27409FR
DyLight 755 NBP2-27409IR
FITC NBP2-27409F
HRP NBP2-27409H
Janelia Fluor 549 NBP2-27409JF549
Janelia Fluor 646 NBP2-27409JF646
PE NBP2-27409PE
PerCP NBP2-27409PCP

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Blogs on IKK alpha.

IKK alpha says "no" to NFk beta
The nuclear factor kappa B (NFkB) is a ubiquitous transcription factor essential for the activation of immune and inflammatory responses. NFkB activity is inhibited when it is associated with IkB proteins in the cell cytoplasm. IkB proteins are phosph...  Read full blog post.

Regulating Immune Response Pathways with IKK beta
IKK beta, also known as IKK2, activates the NFkB complex by phosphorylating the NFkB inhibitor, IkBa. Several transcript variants, some protein-coding and some not, have been found for IKKB. The Nuclear Factor-kappa B (NF-kB) family of transcription f...  Read full blog post.

IKK alpha: Roles in Development, B-cell Survival and ESC Differentiation
Inhibitor of nuclear factor kappa-B kinase subunit alpha (IKK1 alpha) is a serine/threonine kinase that forms a complex with IKK beta and NEMO. It plays an essential role in embryonic skin development. Mice with low levels of IKKa show an increase in...  Read full blog post.

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Bioinformatics

Gene Symbol CHUK
Entrez
Uniprot