CD68/SR-D1 Antibody (FA-11)

Images

 
Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - Mouse spleen cryosection.
Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - Mouse spleen.
Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - Wehi-3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The ...read more
Flow (Intracellular): CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - An intracellular stain was performed on Raw 246.7 cells with CD68/SR-D1 (FA-11) antibody NBP2-33337 (blue) and a matched isotype control MAB006 (orange). ...read more
Western Blot: CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - Expression on J774 cells.
Flow Cytometry: CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - An intracellular stain was performed on Raw264.7 cells with CD68/SR-D1 Antibody (FA-11) NBP2-33337B (blue) and a matched isotype control (orange). Both ...read more
Flow Cytometry: CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - Staining of permeabilised Mouse peritoneal Macrophages cells with Rat anti Mouse CD68 Antibody (Clone FA-11) visualised with F(ab')2 Goat Anti Rat IgG:FITC ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, Flow-IC, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Single-Cell Western
Clone
FA-11
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CD68/SR-D1 Antibody (FA-11) Summary

Immunogen
This CD68/SR-D1 Antibody (FA-11) was developed against purified ConA acceptor glycoproteins from the P815 cell line.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CD68
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Flow Cytometry 1:50-1:100
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Single Cell Western
  • Western Blot 1:100-1:2000
Application Notes
Use in IHC, ICC/IF reported in scientific literature (PMID:34478932). Use in functional reported in scientific literature (PMID: 11085350). . For Flow Cytometry: Use 10 ul of suggested dilution to label 10^6 cells in 100 ul. IHC requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Publications
Read Publications using
NBP2-33337 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD68/SR-D1 Antibody (FA-11)

  • CD_antigen: CD68
  • CD68 antigen
  • CD68 molecule
  • CD68
  • DKFZp686M18236
  • gp110
  • LAMP4
  • macrophage antigen CD68
  • Macrosialin
  • SCARD1
  • scavenger receptor class D, member 1
  • SRD1
  • SR-D1

Background

Human CD68, also known as GP110, LAMP4, Scavenger Receptor D1 (SR-D1) or macrosialin in mouse, encodes a 110-kD transmembrane glycoprotein that belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. Members of the LAMP family include LAMP-1, LAMP-2, dendritic cell (DC)-LAMP (aka CD208), and brain and dendritic cell-associated (BAD)-LAMP (aka LAMP-5). Unlike the two LAMP domains facing the lysosomal lumen in LAMP-1 and LAMP-2, CD68 has a single LAMP domain containing four cystines spaced 36-37 residues apart along with an N-terminal Mucin-like domain. The 354 amino acid (a.a.) human CD68 and 326 a.a. murine ortholog share 80.6% a.a. sequence identity (1).

CD68 is highly expressed in cells of the mononuclear phagocyte system such as macrophages, microglia, osteoclasts, and myeloid dendritic cells (DCs); and is expressed to a lesser extent in lymphoid cells (CD19+ B lymphocytes and CD4+ T lymphocytes), human umbilical cord mesenchymal stem cells (MSCs), fibroblasts, endothelial cells, multiple non-hematopoietic cancer cell lines, and human arterial intimal smooth muscle cells (SMCs). Expression has been also observed in diseased states for granulocytes and neutrophils, in particular basophils from myeloproliferative disorders and intestinal neutrophils from inflammatory bowel disease (IBD), respectively (1).

Although the function of CD68 has yet to be established, it has often been used as an immunohistochemistry (IHC) marker of inflammation and for granular cell tumors (GCTs). CD68+ tumor associated macrophages (TAMs) has been suggested to be a predictive marker for poor cancer prognosis, but a meta-analysis showed the presence of CD68 is not correlated with survival (2). In addition, a role in hepatic malaria infection has been reported based on the finding that peptide P39 binds CD68, considered a receptor for malaria sporozoite, and inhibits parasite entry into Kupffer cells. CD68 was deemed a member of the Scavenger receptor family due to its upregulation in macrophages following inflammatory stimuli, ability to bind modified LDL, phosphatidylserine, and apoptotic cells, as well as shuttling between the plasma membrane and endosomes. CD68 has been linked to atherogenesis based on binding and internalization of its ligand, oxLDL (1).

References

1. Chistiakov, DA, Killingsworth, MC, Myasoedova, VA. Orekhov AN, Bobryshev YV. (2017) CD68/macrosialin: not just a histochemical marker. Lab Invest. 97:4-13. PMID: 27869795

2. Troiano G, Caponio VCA, Adipietro I, Tepedino M, Santoro R, Laino L, Lo Russo L, Cirillo N, Lo Muzio L. (2019) Prognostic significance of CD68+ and CD163+ tumor associated macrophages in head and neck squamous cell carcinoma: A systematic review and meta-analysis. Oral Oncol. 93:66-75. PMID: 31109698.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD68/SR-D1 Antibody (NBP2-33337)(31)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 8 applications: FLOW, Func, ICC/IF, IHC, IHC-FR, IHC-Fr, IHC-P, WB.


Filter By Application
FLOW
(3)
Func
(1)
ICC/IF
(5)
IHC
(2)
IHC-FR
(1)
IHC-Fr
(11)
IHC-P
(4)
WB
(1)
All Applications
Filter By Species
Mouse
(26)
All Species
Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using NBP2-33337 Applications Species
Feng L, Wang Q, Li Y et al. Ablation of Hypoxia-induced mitogenic factor promotes cardiac repair after myocardial infarction by downregulating matrix metalloproteinase-9 expression in macrophage Research Square Sep 15 2021 (IHC-P, Mouse) IHC-P Mouse
Huang J, Fan C, Chen Y Et al. Single-cell RNA-seq reveals functionally distinct biomaterial degradation-related macrophage populations Biomaterials Oct 1 2021 [PMID: 34478932] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Li Y, Dong M, Wang Q et al. HIMF deletion ameliorates acute myocardial ischemic injury by promoting macrophage transformation to reparative subtype Basic research in cardiology Apr 23 2021 [PMID: 33893593] (IHC, Mouse) IHC Mouse
Zhai M, Luan P, Shi Y, et al. Identification of Three Significant Genes Associated with Immune Cells Infiltration in Dysfunctional Adipose Tissue-Induced Insulin-Resistance of Obese Patients via Comprehensive Bioinformatics Analysis International Journal of Endocrinology Jan 22 2021 [PMID: 33564304] (IHC-P, Mouse) IHC-P Mouse
Magarotto F, Sgrò A, Dorigo Hochuli AH, et al. Muscle functional recovery is driven by extracellular vesicles combined with muscle extracellular matrix in a volumetric muscle loss murine model Biomaterials Jan 7 2021 [PMID: 33461058] (ICC/IF, Mouse) ICC/IF Mouse
Tian S, Nakamura J, Hiller S et al. New insights into immunomodulation via overexpressing lipoic acid synthase as a therapeutic potential to reduce atherosclerosis Vascul. Pharmacol. Aug 1 2020 [PMID: 32750408] (IHC-Fr, Mouse) IHC-Fr Mouse
Yang X, Chen S, Shao Z et al. Apolipoprotein E Deficiency Exacerbates Spinal Cord Injury in Mice: Inflammatory Response and Oxidative Stress Mediated by NF-kB Signaling Pathway Front Cell Neurosci May 23 2018 [PMID: 29875635] (ICC/IF, Mouse) ICC/IF Mouse
Chen YF, Chen LH, Yeh YM et al. Minoxidil is a potential neuroprotective drug for paclitaxel-induced peripheral neuropathy. Sci Rep. Mar 28 2017 [PMID: 28349969] (IHC-FR, Mouse) IHC-FR Mouse
Kanters E, Pasparakis M, Gijbels MJ et al. Inhibition of NF-kappaB activation in macrophages increases atherosclerosis in LDL receptor-deficient mice. J Clin Invest. [PMID: 14561702]
te Velde EA, Kusters B, Maass C et al. Histological analysis of defective colonic healing as a result of angiostatin treatment. Exp Mol Pathol. [PMID: 14516772]
Show All 31 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD68/SR-D1 Antibody (NBP2-33337) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-33337AF350
Alexa Fluor 405 NBP2-33337AF405
Alexa Fluor 488 NBP2-33337AF488
Alexa Fluor 532 NBP2-33337AF532
Alexa Fluor 594 NBP2-33337AF594
Alexa Fluor 647 NBP2-33337AF647
Alexa Fluor 700 NBP2-33337AF700
Alexa Fluor 750 NBP2-33337AF750
Allophycocyanin NBP2-33337APC
Allophycocyanin/Cy7 NBP2-33337APCCY7
Biotin NBP2-33337B
DyLight 350 NBP2-33337UV
DyLight 405 NBP2-33337V
DyLight 488 NBP2-33337G
DyLight 550 NBP2-33337R
DyLight 594 NBP2-33337DL594
DyLight 650 NBP2-33337C
DyLight 680 NBP2-33337FR
DyLight 755 NBP2-33337IR
FITC NBP2-33337F
HRP NBP2-33337H
Janelia Fluor 549 NBP2-33337JF549
Janelia Fluor 646 NBP2-33337JF646
PE NBP2-33337PE
PE/Atto594 NBP2-33337PEATT594
PE/Cy5.5 NBP2-33337PECY55
PE/Cy7 NBP2-33337PECY7
PerCP NBP2-33337PCP

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Blogs on CD68/SR-D1.

CD68 (Cluster of differentiation 68, GP110, LAMP4, SCARD1)
CD68 belongs to a growing family of hematopoietic mucin-like molecules known as lysosomal/endosomal-associated membrane glycoproteins (LAMPs). Other LAMP family members included leukosialin, stem cell antigen CD34, and GlyCAM-1. CD68 encodes a 110-kD ...  Read full blog post.

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Bioinformatics

Gene Symbol CD68
Entrez
Uniprot