IKK alpha Antibody (14A231) [Alexa Fluor® 350]

IKK alpha Antibody (14A231) [Alexa Fluor® 350] [NBP2-27409AF350] - Vial of Alexa Fluor 350 conjugated antibody. Alexa Fluor 350 is optimally excited at 346 nm by the UV laser (350 or 355 nm) and has an emission maximum of 442 nm.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: 14A231
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

IKK alpha Antibody (14A231) [Alexa Fluor® 350] Summary

• Immunogen: This antibody was raised against a His-tagged full-length human IKK alpha protein.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHUK
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

New World Monkey

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for IKK alpha Antibody (14A231) [Alexa Fluor® 350]

• CHUK
• conserved helix-loop-helix ubiquitous kinaseIKBKA
• EC 2.7.11
• EC 2.7.11.10
• I-kappa-B kinase 1
• I-kappa-B kinase alpha
• IkappaB kinase
• IkBKA
• IkBKalpha
• IKK alpha
• IKK1
• IKK1I-kappa-B kinase-alpha
• IKK-A
• IKKAIkB kinase alpha subunit
• IKK-alphainhibitor of nuclear factor kappa-B kinase subunit alpha
• NFKBIKA
• NFKBIKAIKK-a kinase
• Nuclear factor NFkappaB inhibitor kinase alpha
• Nuclear factor NF-kappa-B inhibitor kinase alpha
• TCF16
• TCF-16
• Transcription factor 16

Background

IKK-alpha (I-Kappa-B kinase-alpha) is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. Activation of NFKB by TNF requires phosphorylation of IKK-alpha at threonine 23 and serine 176 by AKT1 and NIK respectively (3). It has been demonstrated that IKK-alpha can accumulate in the nucleus after cytokine exposure (4). In the nucleus IKK-alpha interacts with CREB-binding protein in conjunction with RELA to be recruited to the NF-kappa-B-responsive promoters in order to mediate the cytokine-induced phosphorylation and subsequent acetylation of specific residues in histone H3 (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHUK