CD11b/c Antibody - BSA Free

Images

 
Western Blot: CD11b/c Antibody [NB110-40766] - Analysis of CD11b/c in Raw 264.7 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: CD11b/c Antibody [NB110-40766] - CD11 antibody was tested in Raw264.7 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry-Frozen: CD11b/c Antibody [NB110-40766] - 10 um Cryosection of murine acute brain slices stained with CD11b/c antibody (1:300 = 3.3 ug/ml) and Alexa Fluor 555 donkey anti rabbit IgG. Antibody shows ...read more
Flow Cytometry: CD11b/c Antibody [NB110-40766] - A surface stain was performed on RAW 264.7 with NB110-40766 and a matched isotype control. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at ...read more
Immunocytochemistry/ Immunofluorescence: CD11b/c Antibody [NB110-40766] - Phase contrast microscopy images of HFA and brain tumor cells and immunofluorescence images of cultured primary brain tumor cells, AA and HFA. ...read more
Immunohistochemistry: CD11b/c Antibody [NB110-40766] - Staining of human bone marrow, myeloid precursors.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

CD11b/c Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal region (within residues 500-600) of the mouse CD11 (b/c) protein. [Swiss-Prot: P05555]
Localization
Monocytes and granulocytes.
Marker
Microglia Marker, Macrophage Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ITGAM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50 - 1:200
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Western Blot 2-4 ug/ml
Application Notes
In Western blot, a specific band is observed at ~ 160 kDa and an apparent non-specific band is observed at ~ 60 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with citrate buffer (pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Raw 264.7 cells. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 29467366).
Theoretical MW
160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Raw 264.7 Whole Cell Lysate
Control Peptide
CD11b/c Peptide (NB110-40766PEP)
Reviewed Applications
Read 3 Reviews rated 3.7
using
NB110-40766 in the following applications:

Publications
Read Publications using
NB110-40766 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25150592)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 30% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD11b/c Antibody - BSA Free

  • antigen CD11b (p170)
  • CD11 antigen-like family member B
  • CD11b antigen
  • CD11b
  • CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide)
  • Cell surface glycoprotein MAC-1 subunit alpha
  • CR-3 alpha chain
  • CR3AMGC117044
  • integrin alpha-M
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • Leukocyte adhesion receptor MO1
  • MAC-1
  • MAC1A
  • macrophage antigen alpha polypeptide
  • MO1A
  • neutrophil adherence receptor alpha-M subunit
  • Neutrophil adherence receptor
  • SLEB6

Background

CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD11b/c Antibody (NB110-40766)(29)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: FLOW, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
FLOW
(1)
Flow
(1)
ICC/IF
(3)
IHC
(3)
IHC-Fr
(3)
IHC-P
(6)
WB
(12)
All Applications
Filter By Species
Human
(6)
Mouse
(17)
Rat
(2)
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NB110-40766 Applications Species
Pouso-Vazquez E, Bai X, Batalle G et al. Effects of heme oxygenase 1 in the molecular changes and neuropathy associated with type 2 diabetes in mice Biochemical pharmacology Mar 8 2022 [PMID: 35276215] (WB, Mouse) WB Mouse
Li H, Xiao Y, Li Q Et al. The allergy mediator histamine confers resistanceto immunotherapy in cancer patients via activationof the macrophage histamine receptor H1 Cancer cell Nov 18 2021 [PMID: 34822775]
Liu X, Zheng H Modulation of Sirt1 and FoxO1 on Hypothalamic Leptin-Mediated Sympathetic Activation and Inflammation in Diet-Induced Obese Rats Journal of the American Heart Association Jul 20 2021 [PMID: 34259031] (IHC-Fr) IHC-Fr
Redondo A, Riego G, Pol O The Antinociceptive, Antioxidant and Anti-Inflammatory Effects of 5-Fluoro-2-Oxindole during Inflammatory Pain Antioxidants (Basel, Switzerland) Dec 9 2020 [PMID: 33316895] (WB, Mouse) WB Mouse
Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (Flow, Mouse)

Details:
Citation using the Alexa Fluor 488 format of this antibody.
Flow Mouse
Alvarez-Carbonell D, Ye F, Ramanath N et al. Cross-talk between microglia and neurons regulates HIV latency PLoS Pathog. Dec 1 2019 [PMID: 31887215] (ICC/IF, Human) ICC/IF Human
Diaz AF, Polo S, Gallardo N et al. Analgesic and Antidepressant Effects of Oltipraz on Neuropathic Pain in Mice by Modulating Microglial Activation J Clin Med Jun 21 2019 [PMID: 31234342] (WB, Mouse) WB Mouse
Moreno P, Cazuza RA, Mendes-Gomes J, et al. The Effects of Cobalt Protoporphyrin IX and Tricarbonyldichlororuthenium (II) Dimer Treatments and Its Interaction with Nitric Oxide in the Locus Coeruleus of Mice with Peripheral Inflammation Int J Mol Sci May 5 2019 [PMID: 31060340] (WB, Mouse) WB Mouse
Ferreira-Chamorro P, Redondo A, Riego G et al. Sulforaphane Inhibited the Nociceptive Responses, Anxiety- and Depressive-Like Behaviors Associated With Neuropathic Pain and Improved the Anti-allodynic Effects of Morphine in Mice. J. Cancer Res. Clin. Oncol. Nov 21 2018 [PMID: 30542282] (WB, Mouse) WB Mouse
Cruz VH, Arner EN, Wynne KW et al. Loss of Tbk1 kinase activity protects mice from diet-induced metabolic dysfunction. Mol Metab Oct 1 2018 [PMID: 29935921] (IHC-Fr, Mouse) IHC-Fr Mouse
Show All 29 Publications.

Reviews for CD11b/c Antibody (NB110-40766) (3) 3.73

Average Rating: 3.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NB110-40766:
Filter by Applications
IHC-Fr
(1)
IHC
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen CD11b/c NB110-40766
Enlarge
3
reviewed by:
Verified Customer
IHC-Fr Mouse 03/11/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse brain
SpeciesMouse
LotT-1
  4
reviewed by:
Verified Customer
IHC Human 08/04/2010
View

Summary

ApplicationImmunohistochemistry
Sample TestedTumor sections
SpeciesHuman
  4
reviewed by:
System Admin
IHC-P Human 01/30/2009
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman. Mucous membrane of the small intestine
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD11b/c Antibody (NB110-40766). (Showing 1 - 2 of 2 FAQs).

  1. What type of antigen retrieval would you recommend for CD11b/c Antibody (NB110-40766)? I am working with paraformaldehyde-fixed free-floating mouse brain sections (40um).
    • In regards to your inquiry I have spoken with the lab and while we have tested paraffin embedded sections and not free floating, we would recommend citrate buffer pH 6.0. Since they are free floating the lab indicates you can probably incubate for less time with the buffer. We would also recommend using a no antigen retrieval control. Especially if you wanted to try out a few to see which was the optimal one to use. Please see our product specific IHC-P protocol.
  2. I am trying to stain microglia in paraformaldehyde fixed (overnight), frozen sectioned (25 micron) free floating sections of rat brain with NB110-40766. I have yet to produce any significant staining. I have tried both 5 and 10 ug/ml concentrations of primary antibody and have tried the protocol with and without antigen retrieval using heated sodium citrate. Considering this is a membrane bound protein, could the triton be inhibiting antigen/antibody interaction, and would trying something less harsh such as Tween 20 be worthwhile?
    • Since our product will detect an antigen in the extracellular domain of the CD11b protein, I think elimination of the Triton X may help substantially. You can try replacing with 0.1% Tween-20, or just eliminating the detergent all together. We have only been able to validate this antibody in paraffin-embedded tissue sections, so we do not have a recommended protocol for frozen or free-floating sections at this time. However, with a fixation in PFA overnight, I think performing some type of antigen retrieval would be highly recommended. From other protocols for antigen retrieval on frozen tissue sections, heat induced with sodium citrate (pH 6.0) is the most commonly used buffer. I think with the modification to the detergent, you should be able to see a signal in your tissue.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on CD11b/c.

Adhesion Receptor Molecule CD11b/c is the Point of Entry for many Infectious Diseases
Pathogenic microorganisms utilize a variety of cell surface receptors to gain entry into host cells and to bypass the natural defense mechanisms. One of the most prominent receptors used in this fashion is the leukocyte adhesion receptor CD11b/c. This...  Read full blog post.

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Recent Reviews

3.7
5
0
4
2
3
1
2
0
1
0

Verified Customer
03/11/2019
Application: IHC-Fr
Species: Mouse

 
Verified Customer
08/04/2010
Application: IHC
Species: Human

 
System Admin
01/30/2009
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol ITGAM