CD31/PECAM-1 Antibody (JC/70A) Summary
Membrane preparation of a spleen from a patient with hairy cell leukemia.
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot 1:100-1:500
- Flow Cytometry 1:100-1:250
- Immunohistochemistry 1:10-1:25
- Immunohistochemistry-Paraffin 1:10-1:25
- Flow (Cell Surface) 1:100-1:250
- Immunocytochemistry 1:25
This CD31/PECAM1 Antibody (JC/70A) is useful for IHC on paraffin-embedded sections, Flow cytometry and Western blot. It reacts with an ~100 kDa glycoprotein expressed by endothelial cells and ~130 kDa glycoprotein present in platelets. It stains endothelial cells in normal as well as malignant tissues. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in ELISA reported in scientific literature (PMID 8307606). Rabbit reactivity is reported in a publication (PMID: 21533193) which cites the use of this monoclonal antibody, clone JC/70A.
Human and rabbit. Rabbit data from customer review.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. After opening store under sterile conditions.
0.02% Sodium Azide
Protein G purified
Alternate Names for CD31/PECAM-1 Antibody (JC/70A)
- adhesion molecule
- CD31 antigen
- jc70a cd31
- jc70a clone
- PECAM-1, CD31/EndoCAM
- platelet endothelial cell adhesion molecule
- platelet/endothelial cell adhesion molecule
CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells and is specifically concentrated at the junction between them. Therefore, CD31 antibodies are commonly used as endothelial markers to measure angiogenesis in association with tumor recurrence. It is also weakly expressed on many peripheral lymphoid cells and platelets. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas). It is also weakly expressed on many peripheral lymphoid cells and platelets.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for CD31/PECAM-1 Antibody (NB600-562). (Showing 1 - 3 of 3 FAQs).
I have an inquiry about #NB600-562, Anti-CD31/PECAM1 (JC/70A), and wanted to know the recommended IHC-P antigen retrieval protocol?
- Please see our protocols for antigen retrieval. There are different methods of antigen retrieval as you will see; our lab typically recommends the heat induced citrate buffer pH 6.0 antigen retrieval method as the most commonly used in our lab.
What's the possibility of using this antibody for IHC with frozen sections?
- Although this product has only been confirmed for use in IHC-P, it most likely will work with frozen sections as well.
Would this antibody react with samples from Rabbit or not? Because only Hu is listed as its Species, but it is also mentioned that Cross-reacts with Human and Rabbit.
- Yes, this antibody should react with rabbit and will be covered as such in our 100% guarantee.
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