CD31/PECAM-1 Antibody (JC/70A)

Images

 
Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (JC/70A) [NB600-562] - IHC analysis of CD31/PECAM1 in human spleen using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (JC/70A) [NB600-562] - Analysis using the Biotin conjugate of NB600-562. Comparison between mouse (lung, top) and human (prostate, bottom) staining using anti-CD31 ...read more
Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (JC/70A) [NB600-562] - Rabbit Heart, CD31 Stained Red. Image from verified customer review.
Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (JC/70A) [NB600-562] - Formalin fixed paraffin embedded human tonsil stained with CD31 antibody.
Western Blot: CD31/PECAM-1 Antibody (JC/70A) [NB600-562] - Western blot analysis of CD31/PECAM1 expression in 1) Jurkat whole cell lysate, 2) human platelet lysate and 3) U937 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (JC/70A) [NB600-562] - HUVEC cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The ...read more
Flow (Cell Surface): CD31/PECAM-1 Antibody (JC/70A) [NB600-562] - A cell surface stain was performed on HUVEC cells with CD31/PECAM-1 antibody NB600-562 (blue) along with a matched isotype control NBP2-27287 (orange). ...read more
Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (JC/70A) [NB600-562] - Formalin fixed paraffin embedded human tonsil stained with CD31, using ABC method.

Product Details

Summary
Reactivity Hu, RbSpecies Glossary
Applications WB, ELISA, Flow, Flow-CS, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
JC/70A
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CD31/PECAM-1 Antibody (JC/70A) Summary

Immunogen
Membrane preparation of a spleen from a patient with hairy cell leukemia.
Localization
Cell membrane.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PECAM1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA
  • Flow Cytometry 1:100-1:250
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100
  • Flow (Cell Surface) 1:100-1:250
Application Notes
This CD31/PECAM1 Antibody (JC/70A) is useful for IHC on paraffin-embedded sections, Flow cytometry and Western blot. It reacts with an ~100 kDa glycoprotein expressed by endothelial cells and ~130 kDa glycoprotein present in platelets. It stains endothelial cells in normal as well as malignant tissues. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in ELISA reported in scientific literature (PMID 8307606). Rabbit reactivity is reported in a publication (PMID: 21533193) which cites the use of this monoclonal antibody, clone JC/70A.
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB600-562 in the following applications:

Publications
Read Publications using
NB600-562 in the following applications:

Reactivity Notes

Human and rabbit. Rabbit data from customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. After opening store under sterile conditions.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD31/PECAM-1 Antibody (JC/70A)

  • adhesion molecule
  • CD31 antigen
  • CD31
  • CD31/EndoCAM
  • endoCAM
  • FLJ34100
  • FLJ58394
  • GPIIA'
  • jc70a cd31
  • jc70a clone
  • PECA1
  • PECAM1
  • PECAM-1
  • PECAM-1, CD31/EndoCAM
  • platelet endothelial cell adhesion molecule
  • platelet/endothelial cell adhesion molecule

Background

CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells and is specifically concentrated at the junction between them. Therefore, CD31 antibodies are commonly used as endothelial markers to measure angiogenesis in association with tumor recurrence. It is also weakly expressed on many peripheral lymphoid cells and platelets. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas). It is also weakly expressed on many peripheral lymphoid cells and platelets.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Rb
Applications: WB, ELISA, Flow, Flow-CS, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for CD31/PECAM-1 Antibody (NB600-562)(16)

We have publications tested in 2 confirmed species: Human, Rabbit.

We have publications tested in 6 applications: ELISA, FLOW, ICC/IF, IHC, IHC-P, WB.


Filter By Application
ELISA
(1)
FLOW
(1)
ICC/IF
(1)
IHC
(12)
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Human
(12)
Rabbit
(2)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB600-562 Applications Species
Lin CC, Lin AT, Yang AH, Chen KK. Microvascular Injury in Ketamine-Induced Bladder Dysfunction. PLoS ONE. Aug 17 2016 [PMID: 27529746] (ICC/IF) ICC/IF
Strilic B, Yang L, Albarran-Juarez J et al. Tumour-cell-induced endothelial cell necroptosis via death receptor 6 promotes metastasis. Nature. Aug 11 2016 [PMID: 27487218] (IHC, Human) IHC Human
Kuang XY, Jiang XF, Chen C et al. Expressions of glia maturation factor-B by tumor cells and endothelia correlate with neovascularization and poor prognosis in human glioma. Oncotarget. Dec 27 2016 [PMID: 26515590] (IHC, Human) IHC Human
Song Y, Zheng J, Yan M et al. The Effects of Irreversible Electroporation on the Achilles Tendon: An Experimental Study in a Rabbit Model PLoS ONE 2015 Jun 27 [PMID: 26114962] (IHC-P, Rabbit)

Details:
CD31/PECAM-1 antibody (clone JC/70A) was used at 1:50 dilution for IHC-P analysis of irreversible electroporation/IRE-ablated segment, a distal segment and a proximal segment of muscle-tendon complexes from Rabbits (Fig 8).
IHC-P Rabbit
Sun Dongchong, Yang Yong, Wei Zhitao et al. Engineering of pre-vascularized urethral patch with muscle flaps and hypoxia-activated hUCMSCs improves its therapeutic outcome. J Cell Mol Med. 2014 Jan 25 [PMID: 24460735] (IHC) IHC
Song X, Wang J, Zheng T et al. LBH589 Inhibits proliferation and metastasis of hepatocellular carcinoma via inhibition of gankyrin/stat3/akt pathway. Mol Cancer. 2013 Oct 5 [PMID: 24093956] (WB, Human) WB Human
Kalen M, Heikura T, Karvinen H et al. Gamma-secretase inhibitor treatment promotes VEGF-A-driven blood vessel growth and vascular leakage but disrupts neovascular perfusion. PLoS One. 2011 Apr 14 [PMID: 21533193] (IHC, Rabbit) IHC Rabbit
Longatto Filho A, Oliveira TG, Pinheiro C et al. How useful is the assessment of lymphatic vascular density in oral carcinoma prognosis? World J Surg Oncol. 2007 Dec 11 [PMID: 18072963] (IHC, Human) IHC Human
Ding MX, Lin XQ, Fu XY Zhang N, Li JC. Expression of vascular endothelial growth factor-C and angiogenesis in esophageal squamous cell carcinoma World J Gastroenterol. 2006 Jul 28 [PMID: 16874878] (IHC, Human) IHC Human
Elaqoz S, Eqilmez R, Koyuncu A et al. The intratumoral microvessel density and expression of bFGF and nm23-H1 in colorectal cancer. Pathol Oncol Res. 2006 [PMID: 16554912] (IHC, Human) IHC Human
Show All 16 Publications.

Reviews for CD31/PECAM-1 Antibody (NB600-562) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Mouse, Feline, Other.
We have 3 reviews tested in 3 application: ICC, IHC-P, IHC-Fr.

Reviews using NB600-562:
Filter by Applications
ICC
(1)
IHC-P
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
Feline
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry CD31/PECAM-1 NB600-562
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reviewed by:
Anonymous
ICC Feline 05/26/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedEndothelial
SpeciesFeline
LotC-1
Immunohistochemistry-Paraffin CD31/PECAM-1 NB600-562
Enlarge
reviewed by:
Anonymous
IHC-P Mouse 08/12/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testednon-ischemic and ischemic gastrocnemius muscle
SpeciesMouse
LotA
Immunohistochemistry-Frozen CD31/PECAM-1 NB600-562
Enlarge
reviewed by:
Anonymous
IHC-Fr Other 04/28/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRabbit Heart
SpeciesOther
LotA-1
CommentsWhen using paraffin-embedded samples, increase concentration of primary antibody to 1:5 or 1:3.

Blocking

Blocking DetailsBlocked with 5% NGS and 0.3% TritonX-100 in PBS for 60 min at room temperature.

Primary Anitbody

Dilution RatioUsed at 1:10 concentration in a solution of 1% BSA and 0.3% Triton X-100 in PBS. Incubated for 20 hours in wet chamber at 4ºC.

Secondary Antibody

Secondary DescriptionRabbit anti-mouse IgG (H+L)
Secondary Manufacturer Cat#Alexa Fluor Ref# A21063
Secondary Concentration7.5 ug/ml

Details

Detection NotesFluoresence Microscopy. Rhodamine spectrum. Zeiss microscope.
Fixation DetailsFixed with Formaldehyde at 3.7% for 15 min at room temperature.
Wash DescriptionRinsed in PBS 3 times, 5 minutes each.

Comments

CommentsWhen using paraffin-embedded samples, increase concentration of primary antibody to 1:5 or 1:3.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD31/PECAM-1 Antibody (NB600-562). (Showing 1 - 3 of 3 FAQs).

  1. I have an inquiry about #NB600-562, Anti-CD31/PECAM1 (JC/70A), and wanted to know the recommended IHC-P antigen retrieval protocol?
    • Please see our protocols for antigen retrieval. There are different methods of antigen retrieval as you will see; our lab typically recommends the heat induced citrate buffer pH 6.0 antigen retrieval method as the most commonly used in our lab.
  2. What's the possibility of using this antibody for IHC with frozen sections?
    • Although this product has only been confirmed for use in IHC-P, it most likely will work with frozen sections as well.
  3. Would this antibody react with samples from Rabbit or not? Because only Hu is listed as its Species, but it is also mentioned that Cross-reacts with Human and Rabbit.
    • Yes, this antibody should react with rabbit and will be covered as such in our 100% guarantee.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CD31/PECAM-1 Antibody (NB600-562)

Discover related pathways, diseases and genes to CD31/PECAM-1 Antibody (NB600-562). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on CD31/PECAM-1.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Anonymous
05/26/2017
Application: ICC
Species: Feline

Anonymous
08/12/2016
Application: IHC-P
Species: Mouse

Anonymous
04/28/2014
Application: IHC-Fr
Species: Other

Bioinformatics

Gene Symbol PECAM1
Entrez
Uniprot