CD31/PECAM-1 Antibody (JC/70A)


Western Blot: CD31/PECAM-1 Antibody (JC/70A) [NB600-562] - Western blot analysis of CD31/PECAM1 expression in 1) Jurkat whole cell lysate, 2) human platelet lysate and 3) U937 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (JC/70A) [NB600-562] - The CD31/PECAM antibody was tested in Huvec cells at a 1:10 dilution against Dylight 488 (Green). Actin and nuclei were more
Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (JC/70A) [NB600-562] - Rabbit Heart, CD31 Stained Red. Image from verified customer review.
Flow (Cell Surface): CD31/PECAM-1 Antibody (JC/70A) [NB600-562] - A cell surface stain was performed on HUVEC cells with CD31/PECAM-1 antibody NB600-562 (blue) along with a matched isotype control NBP2-27287 (orange). more
Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (JC/70A) [NB600-562] - Formalin fixed paraffin embedded human tonsil stained with CD31, using ABC method.
Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (JC/70A) [NB600-562] - Analysis using the Biotin conjugate of NB600-562. Comparison between mouse (lung, top) and human (prostate, bottom) staining using anti-CD31 more
Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (JC/70A) [NB600-562] - Formalin fixed paraffin embedded human tonsil stained with CD31 antibody.
Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (JC/70A) [NB600-562] - IHC analysis of CD31/PECAM1 in human spleen using DAB with hematoxylin counterstain.

Product Details

Reactivity Hu, RbSpecies Glossary
Applications WB, ELISA, Flow-CS, ICC/IF, IHC, IHC-Fr, IHC-P
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

CD31/PECAM-1 Antibody (JC/70A) Summary

Membrane prepration of a spleen from a patient with hairy cell leukemia.
Cell membrane.
IgG1 Kappa
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
0.1% Sodium Azide
This product is unpurified. The exact concentration of antibody is not quantifiable.
Tissue culture supernatant


  • Western Blot 1:100-1:500
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:25
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:25
  • Flow (Cell Surface) 1 ug/mL - 3 ug/mL
Application Notes
This CD31/PECAM1 Antibody (JC/70A) is useful for Immunohistochemistry on paraffin-embedded sections and Western blot. It reacts with an ~100 kDa glycoprotein expressed by endothelial cells and ~130 kDa glycoprotein present in platelets. It stains endothelial cells in normal as well as malignant tissues. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.Use in ELISA reported in scientific literature (PMID 8307606). Rabbit reactivity is reported in a publication (PMID: 21533193) which cites the use of this monoclonal antibody, clone JC/70A.
Reviewed Applications
Read 2 Reviews rated 5
NB600-562 in the following applications:

Read Publications using
NB600-562 in the following applications:

  • 1 publication
  • 1 publication
  • IHC
    10 publications
  • 1 publication
  • WB
    1 publication

Reactivity Notes

Human and rabbit. Rabbit data from customer review.

Alternate Names for CD31/PECAM-1 Antibody (JC/70A)

  • adhesion molecule
  • CD31 antigen
  • CD31
  • CD31/EndoCAM
  • endoCAM
  • FLJ34100
  • FLJ58394
  • GPIIA'
  • jc70a cd31
  • jc70a clone
  • PECA1
  • PECAM1
  • PECAM-1
  • PECAM-1, CD31/EndoCAM
  • platelet endothelial cell adhesion molecule
  • platelet/endothelial cell adhesion molecule


CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells and is specifically concentrated at the junction between them. Therefore, CD31 antibodies are commonly used as endothelial markers to measure angiogenesis in association with tumor recurrence. It is also weakly expressed on many peripheral lymphoid cells and platelets. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas). It is also weakly expressed on many peripheral lymphoid cells and platelets.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD31/PECAM-1 Antibody (NB600-562)(13)

We have publications tested in 2 confirmed species: Human, Rabbit.

We have publications tested in 5 applications: ELISA, FLOW, IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB600-562 Applications Species
Song Y, Zheng J, Yan M et al. The Effects of Irreversible Electroporation on the Achilles Tendon: An Experimental Study in a Rabbit Model PLoS ONE 2015 Jun 27 [PMID:26114962] (IHC-P, Rabbit) IHC-P Rabbit
Sun Dongchong, Yang Yong, Wei Zhitao et al. Engineering of pre-vascularized urethral patch with muscle flaps and hypoxia-activated hUCMSCs improves its therapeutic outcome. J Cell Mol Med. 2014 Jan 25 [PMID:24460735] (IHC) IHC
Song X, Wang J, Zheng T et al. LBH589 Inhibits proliferation and metastasis of hepatocellular carcinoma via inhibition of gankyrin/stat3/akt pathway. Mol Cancer. 2013 Oct 5 [PMID:24093956] (WB, Human) WB Human
Kalen M, Heikura T, Karvinen H et al. Gamma-secretase inhibitor treatment promotes VEGF-A-driven blood vessel growth and vascular leakage but disrupts neovascular perfusion. PLoS One. 2011 Apr 14 [PMID:21533193] (IHC, Rabbit) IHC Rabbit
Longatto Filho A, Oliveira TG, Pinheiro C et al. How useful is the assessment of lymphatic vascular density in oral carcinoma prognosis? World J Surg Oncol. 2007 Dec 11 [PMID:18072963] (IHC, Human) IHC Human
Ding MX, Lin XQ, Fu XY Zhang N, Li JC. Expression of vascular endothelial growth factor-C and angiogenesis in esophageal squamous cell carcinoma World J Gastroenterol. 2006 Jul 28 [PMID:16874878] (IHC, Human) IHC Human
Elaqoz S, Eqilmez R, Koyuncu A et al. The intratumoral microvessel density and expression of bFGF and nm23-H1 in colorectal cancer. Pathol Oncol Res. 2006 [PMID:16554912] (IHC, Human) IHC Human
Reymond N, Imbert AM, Devilard E et al. DNAM-1 and PVR regulate monocyte migration through endothelial junctions. J Exp Med. 2004 May 17 [PMID:15136589] (IHC, FLOW, Human) IHC, FLOW Human
Zurita AJ, Troncoso P, Cardo-Vila M et al. Combinatorial screenings in patients: the interleukin-11 receptor alpha as a candidate target in the progression of human prostate cancer. Cancer Res. 2004 Jan 15 [PMID:14744752] (IHC, Human) IHC Human
Hambrecht R, Adams V, Erbs S. Regular physical activity improves endothelial function in patients with coronary artery disease by increasing phosphorylation of endothelial nitric oxide synthase. Circulation. 2003 Jul 1 [PMID:12810615] (IHC, Human) IHC Human
Show All 13 Publications.

Reviews for CD31/PECAM-1 Antibody (NB600-562) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Other.
We have 2 review tested in 1 application: IHC-Fr.

Reviews using NB600-562:
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Images Ratings Applications Species Date Details
 CD31/PECAM-1 NB600-562
reviewed by:
Mouse 08/12/2016


Sample Testednon-ischemic and ischemic gastrocnemius muscle
Immunohistochemistry-Frozen CD31/PECAM-1 NB600-562
reviewed by:
IHC-Fr Other 04/28/2014


Sample TestedRabbit Heart
CommentsWhen using paraffin-embedded samples, increase concentration of primary antibody to 1:5 or 1:3.


Blocking DetailsBlocked with 5% NGS and 0.3% TritonX-100 in PBS for 60 min at room temperature.

Primary Anitbody

Dilution RatioUsed at 1:10 concentration in a solution of 1% BSA and 0.3% Triton X-100 in PBS. Incubated for 20 hours in wet chamber at 4ºC.

Secondary Antibody

Secondary DescriptionRabbit anti-mouse IgG (H+L)
Secondary Manufacturer Cat#Alexa Fluor Ref# A21063
Secondary Concentration7.5 ug/ml


Detection NotesFluoresence Microscopy. Rhodamine spectrum. Zeiss microscope.
Fixation DetailsFixed with Formaldehyde at 3.7% for 15 min at room temperature.
Wash DescriptionRinsed in PBS 3 times, 5 minutes each.


CommentsWhen using paraffin-embedded samples, increase concentration of primary antibody to 1:5 or 1:3.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD31/PECAM-1 Antibody (NB600-562). (Showing 1 - 3 of 3 FAQs).

  1. I have an inquiry about #NB600-562, Anti-CD31/PECAM1 (JC/70A), and wanted to know the recommended IHC-P antigen retrieval protocol?
    • Please see our protocols for antigen retrieval. There are different methods of antigen retrieval as you will see; our lab typically recommends the heat induced citrate buffer pH 6.0 antigen retrieval method as the most commonly used in our lab.
  2. What's the possibility of using this antibody for IHC with frozen sections?
    • Although this product has only been confirmed for use in IHC-P, it most likely will work with frozen sections as well.
  3. Would this antibody react with samples from Rabbit or not? Because only Hu is listed as its Species, but it is also mentioned that Cross-reacts with Human and Rabbit.
    • Yes, this antibody should react with rabbit and will be covered as such in our 100% guarantee.

Secondary Antibodies


Isotype Controls

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Gene Symbol PECAM1

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