CD11b/c Antibody [DyLight 594]

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 594

CD11b/c Antibody [DyLight 594] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: A synthetic peptide made to an internal region (within residues 500-600) of the mouse CD11 (b/c) protein. [Swiss-Prot: P05555]
• Localization: Monocytes and granulocytes.
• Marker: Microglia Marker, Macrophage Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ITGAM
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB110-40766. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25150592)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for CD11b/c Antibody [DyLight 594]

• antigen CD11b (p170)
• CD11 antigen-like family member B
• CD11b antigen
• CD11b
• CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide)
• Cell surface glycoprotein MAC-1 subunit alpha
• CR-3 alpha chain
• CR3AMGC117044
• integrin alpha-M
• integrin, alpha M (complement component 3 receptor 3 subunit)
• Leukocyte adhesion receptor MO1
• MAC-1
• MAC1A
• macrophage antigen alpha polypeptide
• MO1A
• neutrophil adherence receptor alpha-M subunit
• Neutrophil adherence receptor
• SLEB6

Background

CD11b, also known as Integrin alpha M, is one half of the heterodimeric integrin alpha-M beta-2 molecule. This protein mediates inflammation by regulating leukocyte adhesion and migration and has been implicated in several immune processes such as phagocytosis, cell-mediated cytotoxicity, chemotaxis and cellular activation. CD11b is expressed on the surface of many leukocytes involved in the innate immune system, including monocytes, granulocytes, macrophages, and natural killer cells. Mutations in the CD11b protein have been assosicated with the autoimmune disease lupus, and CD11b antibodies are therefore commonly in immunology studies.

CD11c, also known as Integrin alpha X, is a type I transmembrane protein found at high levels on most human dendritic cells, but also on monocytes, macrophages, neutrophils, and some B cells that induces cellular activation and helps trigger neutrophil respiratory burst; expressed in hairy cell leukemias, acute nonlymphocytic leukemias, and some B-cell chronic lymphocytic leukemias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGAM