IKK beta Antibody (10AG2)


Western Blot: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of A) human Daudi, B) HeLa, and C) mouse NIH3T3 lysate probed with IKKbeta antibody at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: IKK beta Antibody (10AG2) [NB100-56509] - U-87 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells ...read more
Immunohistochemistry-Paraffin: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of IKK beta clone 10AG2 antibody. The staining was developed using HRP ...read more
Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of PE conjugate of NB100-56509. An intracellular stain was performed on Jurkat cells with IKK Beta antibody (10AG2) NB100-56509PE (blue) and a matched ...read more
Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56509. Staining of IKKbeta in HEK 293 cells using 0.1 ug of Alexa Fluor 488-conjugated antibody. Green ...read more
Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis using Alexa Fluor (R) 647 conjugate of NB100-56509. An intracellular stain was performed on HeLa cells with IKK beta antibody (10AG2) NB100-56509 (blue) ...read more
Simple Western: IKK beta Antibody (10AG2) [NB100-56509] - Lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, IB, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
1.0 mg/ml

Order Details

IKK beta Antibody (10AG2) Summary

Full-length recombinant human IKK beta protein (NP_001547).
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 2 - 4 ug/ml
  • Simple Western 1:12.5
  • Flow Cytometry 0.25 - 0.5 ug/ 10^6 cells
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 2-5 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10 - 1:500
  • CyTOF-ready
Application Notes
Useful in IHC-P See Page et al. Use in ICC/IF reported in scientific literature (PMID 24825920).Use in immunoblotting reported in scientific literature (PMID: 20103608).

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
Theoretical MW
87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
IKK beta Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
NB100-56509 in the following applications:

Read Publications using
NB100-56509 in the following applications:

  • 2 publications
  • 1 publication
  • IP
    5 publications
  • WB
    39 publications

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for IKK beta Antibody (10AG2)

  • EC 2.7.11
  • EC
  • I-kappa-B kinase 2
  • I-kappa-B-kinase beta
  • IkBKB
  • IKK beta
  • IKK2
  • IKK2FLJ38368
  • IKK-B
  • IKK-betaFLJ36218
  • IKKBFLJ40509
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
  • inhibitor of nuclear factor kappa-B kinase subunit beta
  • MGC131801
  • Nuclear factor NF-kappa-B inhibitor kinase beta


NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli. Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
Species: Hu, Rt, Rb
Applications: WB, Flow, IHC, IHC-P, KO
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, KO
Species: Mu, Bv
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
Species: Hu, Rt, Po, Ca, Pm, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Bv, Pa
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IB, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Supplier Logo

Publications for IKK beta Antibody (NB100-56509)(54)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC-P, IP, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 54. Show All 54 Publications.
Publications using NB100-56509 Applications Species
Best KT, Nichols AEC, Knapp E et al. NF-kappaB activation persists into the remodeling phase of tendon healing and promotes myofibroblast survival Nat Commun Nov 13 2020 [PMID: 33203721] (WB, Human, Mouse) WB Human, Mouse
De S, Karim F, Kiessu E et al. Mechanism of dysfunction of human variants of the IRAK4 kinase and a role for its kinase activity in interleukin-1 receptor signaling J. Biol. Chem. Aug 16 2018 [PMID: 30115681] (WB, Human) WB Human
Abdi K, Lai CH, Paez-Gonzalez P et al. Uncovering inherent cellular plasticity of multiciliated ependyma leading to ventricular wall transformation and hydrocephalus. Nat Commun Apr 25 2018 [PMID: 29695808] (Mouse) Mouse
Kirkley KS, Walton KD, Duncan C, Tjalkens RB. Spontaneous Development of Cutaneous Squamous Cell Carcinoma in Mice with Cell-specific Deletion of Inhibitor of kB Kinase 2 Comp. Med. 2017 Oct 01 [PMID: 28935002] (IHC-P, Mouse) IHC-P Mouse
Cleary MM, Mansoor A, Settelmeyer T et al. NFkB signaling in alveolar rhabdomyosarcoma Dis Model Mech 2017 Sep 01 [PMID: 28883017] (WB, Mouse) WB Mouse
Liu J, Ibi D, Taniguchi K et al. Inflammation Improves Glucose Homeostasis through IKKbeta-XBP1s Interaction. Cell. Nov 3 2016 [PMID: 27814504] (Human) Human
Reid M A, Lowman X H et al. IKK bet promotes metabolic adaptation to glutamine deprivation via phosphorylation and inhibition of PFKFB3. Genes Dev Aug 15 2016 [PMID: 27585591] (WB, Human) WB Human
Buhrmann C, Busch F, Shayan P, Shakibaei M. Sirtuin-1 (SIRT1) Is Required for Promoting Chondrogenic Differentiation of Mesenchymal Stem Cells. J. Biol. Chem. 2014 Jun 24 [PMID: 24962570] (IP, WB, Human)

IP & WB: Fig 5C, Primary chondrocytes and mesenchymal stem cells. The IKK beta mAb immunoprecipitated an active immune complex as shown by an immune complex assay (Fig 5C).
IP, WB Human
Das U, Manna K, Sinha M et al. Role of ferulic Acid in the amelioration of ionizing radiation induced inflammation: a murine model. PLoS ONE. 2014 May 23 [PMID: 24854039] (WB, Mouse)

Liver, Fig 7.
WB Mouse
Paul S, Traver MK, Kashyap AK et al. T Cell Receptor Signals to NF-kB Are Transmitted by a Cytosolic p62-Bcl10-Malt1-Ikk Signalosome. Sci Signal. 2014 May 14 [PMID: 24825920] (ICC/IF, Mouse)

D10 mouse T-helper cells, Fig S1B
ICC/IF Mouse
Show All 54 Publications.

Review for IKK beta Antibody (NB100-56509) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-56509:
Filter by Applications
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
Western Blot IKK beta NB100-56509
reviewed by:
WB Mouse 05/06/2014


ApplicationWestern Blot
Sample TestedCell lysate


Blocking Details5% Milk / 1x TBST 2 hours @ RT

Primary Anitbody

Dilution Ratio1:1000 1 hour @ RT 5% Milk / 1x TBST

Secondary Antibody

Secondary DescriptionGoat anti-Mouse Ig G HRP
Secondary Manufacturer Cat#Thermo 31430
Secondary Concentration1:10000


Detection NotesECL, film, less than 5 min

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IKK beta Antibody (NB100-56509) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-56509AF350
Alexa Fluor 405 NB100-56509AF405
Alexa Fluor 488 NB100-56509AF488
Alexa Fluor 532 NB100-56509AF532
Alexa Fluor 594 NB100-56509AF594
Alexa Fluor 647 NB100-56509AF647
Alexa Fluor 700 NB100-56509AF700
Alexa Fluor 750 NB100-56509AF750
Allophycocyanin NB100-56509APC
DyLight 350 NB100-56509UV
DyLight 405 NB100-56509V
DyLight 488 NB100-56509G
DyLight 550 NB100-56509R
DyLight 594 NB100-56509DL594
DyLight 650 NB100-56509C
DyLight 680 NB100-56509FR
DyLight 755 NB100-56509IR
FITC NB100-56509F
HRP NB100-56509H
Janelia Fluor 549 NB100-56509JF549
Janelia Fluor 646 NB100-56509JF646
PE NB100-56509PE
PerCP NB100-56509PCP

Additional IKK beta Products

Bioinformatics Tool for IKK beta Antibody (NB100-56509)

Discover related pathways, diseases and genes to IKK beta Antibody (NB100-56509). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IKK beta Antibody (NB100-56509)

Discover more about diseases related to IKK beta Antibody (NB100-56509).

Pathways for IKK beta Antibody (NB100-56509)

View related products by pathway.

PTMs for IKK beta Antibody (NB100-56509)

Learn more about PTMs related to IKK beta Antibody (NB100-56509).

Research Areas for IKK beta Antibody (NB100-56509)

Find related products by research area.

Blogs on IKK beta.

Regulating Immune Response Pathways with IKK beta
IKK beta, also known as IKK2, activates the NFkB complex by phosphorylating the NFkB inhibitor, IkBa. Several transcript variants, some protein-coding and some not, have been found for IKKB. The Nuclear Factor-kappa B (NF-kB) family of transcription f...  Read full blog post.

IKK alpha: Roles in Development, B-cell Survival and ESC Differentiation
Inhibitor of nuclear factor kappa-B kinase subunit alpha (IKK1 alpha) is a serine/threonine kinase that forms a complex with IKK beta and NEMO. It plays an essential role in embryonic skin development. Mice with low levels of IKKa show an increase in...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Recent Reviews


Application: WB
Species: Mouse


Gene Symbol IKBKB