IKK beta Antibody (10AG2) - BSA Free

Western Blot: IKK beta Antibody (10AG2) [NB100-56509] - Representative western blot of IKKB (E) in monocytes with quantification on the right. All data are representative of 5 separate experiments. Unpaired student t-test. ***p < 0.001. Image collected and cropped by CiteAb from the following publication (doi.org/10.3389/fimmu.2020.01478) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of IKK beta Antibody in HeLa cells. Antibody dilution 5 ug/ml. Image from verified customer review.

Immunohistochemistry-Paraffin: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of IKK beta clone 10AG2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of PE conjugate of NB100-56509. An intracellular stain was performed on Jurkat cells with IKK Beta antibody (10AG2) NB100-56509PE (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells were fixed with 4% PFA and then permeablized wi

Western Blot: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of A) human Daudi, B) HeLa, and C) mouse NIH3T3 lysate probed with IKKbeta antibody at 2 ug/ml.

Immunocytochemistry/Immunofluorescence: IKK beta Antibody (10AG2) [NB100-56509] - U-87 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-IKK beta Antibody (10AG2) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56509. Staining of IKKbeta in HEK 293 cells using 0.1 ug of Alexa Fluor 488-conjugated antibody. Green histogram represents the isotype control , red represents the IKKbeta antibody.

Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis using Alexa Fluor (R) 647 conjugate of NB100-56509. An intracellular stain was performed on HeLa cells with IKK beta antibody (10AG2) NB100-56509 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. 1 ug of antibody was added to 100 uL of staining buffer and cells were incubated for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Simple Western: IKK beta Antibody (10AG2) [NB100-56509] - Lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, Flow, IB, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
• Clone: 10AG2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

IKK beta Antibody (10AG2) - BSA Free Summary

• Immunogen: Full-length recombinant human IKK beta protein (NP_001547).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IKBKB
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 - 0.5 ug/ 10^6 cells
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 2-5 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Simple Western 1:12.5
  • Western Blot 2 - 4 ug/ml
• Application Notes: Useful in IHC-P See Page et al. Use in ICC/IF reported in scientific literature (PMID 24825920).Use in immunoblotting reported in scientific literature (PMID: 20103608).
  
  In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
• Theoretical MW: 87 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34333329)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for IKK beta Antibody (10AG2) - BSA Free

• EC 2.7.11
• EC 2.7.11.10
• I-kappa-B kinase 2
• I-kappa-B-kinase beta
• IkBKB
• IKK beta
• IKK2
• IKK2FLJ38368
• IKK-B
• IKK-betaFLJ36218
• IKKBFLJ40509
• inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• inhibitor of nuclear factor kappa-B kinase subunit beta
• MGC131801
• NFKBIKB
• NFKBIKBFLJ33771
• Nuclear factor NF-kappa-B inhibitor kinase beta

Background

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli. Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IKK beta Antibody (NB100-56509)(57)

Publications using NB100-56509

• Karim K, Giribabu N, Salleh N Marantodes pumilum Var Alata (Kacip Fatimah) ameliorates derangement in RANK/RANKL/OPG pathway and reduces inflammation and oxidative stress in the bone of estrogen-deficient female rats with type-2 diabetes Phytomedicine : international journal of phytotherapy and phytopharmacology 2021-07-18 [PMID: 34333329] (IF/IHC)
• Best KT, Nichols AEC, Knapp E et al. NF-kappaB activation persists into the remodeling phase of tendon healing and promotes myofibroblast survival Nat Commun 2020-11-13 [PMID: 33203721] (WB, Human, Mouse)
• Rahtes, A & Li, L. Polarization of Low-Grade Inflammatory Monocytes Through TRAM-Mediated Up-Regulation of Keap1 by Super-Low Dose Endotoxin. Front Immunol 2020-08-09 [PMID: 32765513] (IF/IHC, Human)
• De S, Karim F, Kiessu E et al. Mechanism of dysfunction of human variants of the IRAK4 kinase and a role for its kinase activity in interleukin-1 receptor signaling J. Biol. Chem. 2018-08-16 [PMID: 30115681] (WB, Human)
• Abdi K, Lai CH, Paez-Gonzalez P et al. Uncovering inherent cellular plasticity of multiciliated ependyma leading to ventricular wall transformation and hydrocephalus. Nat Commun 2018-04-25 [PMID: 29695808] (Mouse)
• Kirkley KS, Walton KD, Duncan C, Tjalkens RB. Spontaneous Development of Cutaneous Squamous Cell Carcinoma in Mice with Cell-specific Deletion of Inhibitor of kB Kinase 2 Comp. Med. 2017-10-01 [PMID: 28935002] (IHC-P, Mouse)
• Cleary MM, Mansoor A, Settelmeyer T et al. NFkB signaling in alveolar rhabdomyosarcoma Dis Model Mech 2017-09-01 [PMID: 28883017] (WB, Mouse)
• Liu J, Ibi D, Taniguchi K et al. Inflammation Improves Glucose Homeostasis through IKKbeta-XBP1s Interaction. Cell. 2016-11-03 [PMID: 27814504] (Human)
• Reid M A, Lowman X H et al. IKK bet promotes metabolic adaptation to glutamine deprivation via phosphorylation and inhibition of PFKFB3. Genes Dev 2016-08-15 [PMID: 27585591] (WB, Human)
• Lim Y, Ooi K, Subramaniam M, et al. Apoptotic and cytostatic actions of maslinic acid in colorectal cancer cells through possible IKK-beta inhibition Asian Pacific Journal of Tropical Biomedicine 2021-01-21
• Buhrmann C, Busch F, Shayan P, Shakibaei M. Sirtuin-1 (SIRT1) Is Required for Promoting Chondrogenic Differentiation of Mesenchymal Stem Cells. J. Biol. Chem. 2014-06-24 [PMID: 24962570] (WB, IP, Human)
  
  Details:
  IP & WB: Fig 5C, Primary chondrocytes and mesenchymal stem cells. The IKK alpha mAb immunoprecipitated an active immune complex as shown by an immune complex assay (Fig 5C).
• Das U, Manna K, Sinha M et al. Role of ferulic Acid in the amelioration of ionizing radiation induced inflammation: a murine model. PLoS One 2014-05-24 [PMID: 24854039] (WB, Mouse)
• Paul S, Traver MK, Kashyap AK et al. T Cell Receptor Signals to NF-kB Are Transmitted by a Cytosolic p62-Bcl10-Malt1-Ikk Signalosome. Sci Signal. 2014-05-14 [PMID: 24825920] (ICC/IF, Mouse)
  
  Details:
  D10 mouse T-helper cells, Fig S1B
• Breuer R, Becker MS, Brechmann M et al. The PP2A phosphatase regulatory subunit B56-gamma mediates suppression of T-cell receptor (TCR)-induced nuclear factor-kappa B (NF-kB) activity. J. Biol. Chem. 2014-04-09 [PMID: 24719332] (WB, Human)
  
  Details:
  Jurkat, Fig 3.
• Gray CM, Remouchamps C, McCorkell KA et al. Noncanonical NF-kB Signaling Is Limited by Classical NF-kB Activity. Sci Signal. 2014-02-05 [PMID: 24497610] (WB, Mouse)
  
  Details:
  Mouse embryo fibroblasts (MEFs) retrovirally transduced with various constructs, Figs 1-7.
• Ren T, Takahashi Y, Liu X et al. HTLV-1 Tax deregulates autophagy by recruiting autophagic molecules into lipid raft microdomains. Oncogene 2013-12-23 [PMID: 24362528] (WB, Human)
• Bimonte S, Barbieri A, Palma G et al. Curcumin inhibits tumor growth and angiogenesis in an orthotopic Mouse model of human pancreatic cancer. Biomed Res Int 2013-01-01 [PMID: 24324975] (WB, Human)
  
  Details:
  PaCa-2 (human pancreatic cell line) orthotopic mouse model of human pancreatic cancer, Fig 3C.
• Delhase M, Kim SY, Lee H et al. TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2. Proc Natl Acad Sci U S A. 2012-01-24 [PMID: 22203995]
• Page A, Cascallana JL, Casanova ML et al. IKKB overexpression leads to pathologic lesions in stratified epithelia and exocrine glands and to tumoral transformation of oral epithelia. Mol Cancer Res. 2011-10-01 [PMID: 21821676] (WB, Mouse)
  
  Details:
  IkkB (IMG-129A). WB: Various k5-IkkB transgenic mouse organs, Fig 1A. IHC (paraffin): 5-IkkB mouse exocrine and lactating mammary glands, Fig 1C & 1D.
• Phromnoi K, Reuter S, Sung B et al. A novel pentamethoxyflavone down-regulates tumor cell survival and proliferative and angiogenic gene products through inhibition of IkB kinase activation and sensitizes tumor cells to apoptosis by cytokines and chemotherapeutic agents. Mol Pharmacol. 2011-02-01 [PMID: 20930110] (WB, Human)
  
  Details:
  KBM-5 cells: 1. FLIP (IMG-116-1, -2): WB (Fig 4A) 2. IKKb/IKK2 (IMG-129A): IP Kinase Assay (Fig 2D), IP/WB (Fig 2D). Note, the antibody IP'd an active kinase. The antibody co'IP'd IKKa. 3. IKKa (IMG-136a): WB (Figs 2C, 2D). Note Fig 2D was generated with an IP (IKKb, IMG-129A)/WB (IKKa, IMG-136a) assay. Note: The KBM-5 (human chronic myeloid leukemia) cells were incubated with PMF then treated with TNF-alpha, Figs 2C, 2D, 4A.
• Harikumar KB, Sung B, Pandey MK et al. Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway Mol Pharmacol 2010-05-01 [PMID: 20103608]
  
  Details:
  This citation used the Alexa Fluor 488 version of this antibody.
• Jin HS, Lee DH, Kim DH et al. cIAP1, cIAP2, and XIAP act cooperatively via nonredundant pathways to regulate genotoxic stress-induced nuclear factor-kappaB activation. Cancer Res. 2009-03-01 [PMID: 19223549] (IP)
  
  Details:
  IP (HeLa cells), Fig. 2c.
• Koch Katherine S, Maeda Shin, He Guobin et al. Targeted deletion of hepatocyte Ikkbeta confers growth advantages. Biochem Biophys Res Commun. 2009-03-06 [PMID: 19171122] (ICC/IF, Mouse)
• Huang J, Ren T, Guan H et al. HTLV-1 Tax is a critical lipid raft modulator that hijacks IkappaB kinases to the microdomains for persistent activation of NF-kappaB. J Biol Chem. 2009-03-06 [PMID: 19129196] (WB, Human)
  
  Details:
  Antibodies cited: 1. IMX-5142 (IkBa- Sera): WB (human T cell lines), Fig. 1B. 2. IMG-136A (Ikka/Ikk1): WB (human T cell lines), Figs. 1E,D,G; 2A; 3D,E,F,G; 4B,F; 6C,D; 7C,D,G. 3. IMG-129A (Ikkb/Ikk2): WB (human T cell lines), Figs. 1E,D,G; 2A; 3D,E,F,G; 4
• Kunnumakkara AB, Ichikawa H, Anand P et al. Coronarin D, a labdane diterpene, inhibits both constitutive and inducible nuclear factor-kappa B pathway activation, leading to potentiation of apoptosis, inhibition of invasion, and suppression of osteoclastogenesis. Mol Cancer Ther. 2008-10-01 [PMID: 18852134] (WB, Human)
  
  Details:
  IKK-alpha/IKK1 (IMG-136A): WB (human KMB-5 cells), Fig. 2C. 2. IKKb/IKK2 (IMG-129A): WB (human KMB-5 cells), Fig. 2C.
• Pandey MK, Sung B, Kunnumakkara AB et al. Berberine modifies cysteine 179 of IkappaBalpha kinase, suppresses nuclear factor-kappaB-regulated antiapoptotic gene products, and potentiates apoptosis. Cancer Res. 2008-07-01 [PMID: 18593939] (WB, Human)
  
  Details:
  IMG-136A (IKKa/IKK1): WB (human multiple myeloma U266 cells), Fig. 3A, C. 2. IMG-129A (IKKb/IKK1): WB (human multiple myeloma U266 cells), Fig. 3A, C.
• Kasinski AL, Du Y, Thomas SL et al. Inhibition of IkappaB kinase-nuclear factor-kappaB signaling pathway by 3,5-bis(2-flurobenzylidene)piperidin-4-one (EF24), a novel monoketone analog of curcumin. Mol Pharmacol. 2008-09-01 [PMID: 18577686] (WB, Human)
  
  Details:
  Antibodies cited: 1. Ikka/Ikk1 (IMG-136A): IP (human A549 cells), immunoprecipitated cytoplasmic extracts were used for Ikk kinase assays. 2. Ikkb/Ikk2 (IMG-129A):WB (human A549 cells), Fig. 5a; 6a,b.
• Lamothe B, Webster WK, Gopinathan A et al. TRAF6 ubiquitin ligase is essential for RANKL signaling and osteoclast differentiation. Biochem Biophys Res Commun. 2007-08-10 [PMID: 17572386] (WB)
  
  Details:
  Antibodies cited [WB (pRS and Ubc13 knockdown clones), Supplementary Fig. S1]: 1. Ikkb [IMG-129A] 2. RANk [IMG-128] WB (HEk293 cells transfected with RANk), Fig. 2a WB (HEk293 cells transfected with RANk), Fig. 2b
• Beraza N, Ludde T, Assmus U et al. Hepatocyte-specific IKK gamma/NEMO expression determines the degree of liver injury. Gastroenterology. 2007-06-01 [PMID: 17570222] (IP, Mouse)
  
  Details:
  Products cited: 1. IMG-129A (Ikkb/Ikk2): IP (mouse liver proteins), Fig. 1E; Tab. 1. 2. IMG-136A (Ikka/Ikk1): WB (mouse live proteins), Fig. 1E.
• Vunta H, Davis F, Palempalli UD et al. The anti-inflammatory effects of selenium are mediated through 15-deoxy-Delta12,14-prostaglandin J2 in macrophages. J Biol Chem. 2007-06-22 [PMID: 17439952]
  
  Details:
  WB (LPS-stimulated RAW 264.7 macrophages), Fig. 2d WB (LPS-stimulated RAW 264.7 cells), Fig. 6a WB (LPS-stimulated RAW 264.7 cells treated with 14C-arachidonic acid), Fig. 6b IP (LPS-stimulated RAW 264.7 cells), Fig. 3c IP (LPS-stimulated RAW 264.7 cells)
• Leis Hugo, Sanchis Ana, Perez Paloma. Deletion of the N-terminus of IKKgamma induces apoptosis in keratinocytes and impairs the AKT/PTEN signaling pathway. Exp Cell Res. 2007-02-15 [PMID: 17184772] (Mouse)
• Jackson-Bernitsas DG, Ichikawa H, Takada Y et al. Evidence that TNF-TNFR1-TRADD-TRAF2-RIP-TAK1-IKK pathway mediates constitutive NF-kappaB activation and proliferation in human head and neck squamous cell carcinoma. Oncogene. 2007-03-01 [PMID: 16953224] (WB)
• Sethi G, Ahn KS, Sandur SK et al. Indirubin enhances tumor necrosis factor-induced apoptosis through modulation of nuclear factor-kappa B signaling pathway. J Biol Chem. 2006-08-18 [PMID: 16785236] (WB)
• Sandur SK, Ichikawa H, Sethi G et al. Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone) suppresses NF-kappaB activation and NF-kappaB-regulated gene products through modulation of p65 and IkappaBalpha kinase activation, leading to potentiation of apoptosis induced by cytokine and chemotherape J Biol Chem. 2006-06-23 [PMID: 16624823] (WB)
  
  Details:
  IKKa [IMG-136A] IP (TNF-treated KBM-5 cells incubated with plumbagin), Fig. 4c WB (TNF-treated KBM-5 cells incubated with plumbagin), Fig. 4c 2. IKKb [IMG-129A] WB (TNF-treated KBM-5 cells incubated with plumbagin), Fig. 4c 3. cFLIP [IMG-116] WB (TNF-treated KBM-5 cells incubated with plumbagin), Fig. 5c.
• Ichikawa H, Aggarwal BB. Guggulsterone inhibits osteoclastogenesis induced by receptor activator of nuclear factor-kappaB ligand and by tumor cells by suppressing nuclear factor-kappaB activation. Clin Cancer Res. 2006-01-15 [PMID: 16428513] (WB)
• Nenci A, Huth M, Funteh A et al. Skin lesion development in a mouse model of incontinentia pigmenti is triggered by NEMO deficiency in epidermal keratinocytes and requires TNF signaling. Hum Mol Genet. 2006-02-15 [PMID: 16399796]
  
  Details:
  IKKa [IMG-136A] WB (mouse P0 epidermis, keratinocytes, wt and NEMO-deficient MEF), Fig. 1a 2. IKKb [IMG-136A] WB (mouse P0 epidermis, keratinocytes, wt and NEMO-deficient MEF), Fig. 1a
• Takada Y, Gillenwater A, Ichikawa H et al. Suberoylanilide hydroxamic acid potentiates apoptosis, inhibits invasion, and abolishes osteoclastogenesis by suppressing nuclear factor-kappaB activation. J Biol Chem. 2006-03-03 [PMID: 16377638] (WB)
  
  Details:
  Antibodies used: 1. IKKa [IMG-136A] WB (TNF-treated KBM-5 cells incubated with SAHA and ALLN), Fig. 5g IP (TNF-treated KBM-5 cells incubated with SAHA and ALLN), Fig. 5g 2. IKKb [IMG-129A] WB (TNF-treated KBM-5 cells incubated with SAHA and ALLN), Fig. 5g
• Herrmann O, Baumann B, de Lorenzi R et al. IKK mediates ischemia-induced neuronal death. Nat Med. 2005-12-01 [PMID: 16286924] (WB, Mouse)
  
  Details:
  WB (mouse forebrain) Figs. 2a, 2b, 2c WB (mouse brain), Fig. 3c
• Shishodia S, Gutierrez AM, Lotan R et al. N-(4-hydroxyphenyl)retinamide inhibits invasion, suppresses osteoclastogenesis, and potentiates apoptosis through down-regulation of I(kappa)B(alpha) kinase and nuclear factor-kappaB-regulated gene products. Cancer Res. 2005-10-15 [PMID: 16230421] (WB)
  
  Details:
  [IP (4-HPR and TNF-treated H1299 cells), Fig. 5b IP (TNF-treated H1299 cells), Fig. 5c]: 1. IKKa [IMG-136A] 2. IKKb [IMG-129A] WB (4-HPR and TNF-treated H1299 cells), Fig. 5b WB (4-HPR and TNF-treated H1299 cells), Fig. 5c
• Shishodia S, Amin HM, Lai R et al. Curcumin (diferuloylmethane) inhibits constitutive NF-kappaB activation, induces G1/S arrest, suppresses proliferation, and induces apoptosis in mantle cell lymphoma. Biochem Pharmacol. 2005-09-01 [PMID: 16023083]
• Ichikawa H, Takada Y, Murakami A, Aggarwal BB. Identification of a novel blocker of I kappa B alpha kinase that enhances cellular apoptosis and inhibits cellular invasion through suppression of NF-kappa B-regulated gene products. J Immunol. 2005-06-01 [PMID: 15905586] (WB)
• Luedde T, Assmus U, Wustefeld T et al. Deletion of IKK2 in hepatocytes does not sensitize these cells to TNF-induced apoptosis but protects from ischemia/reperfusion injury. J Clin Invest. 2005-04-01 [PMID: 15776110]
  
  Details:
  Antibodies cited: 1. Ikka [IMG-136A] WB (mouse liver IP'd with anti-NEMO), Fig. 4a WB (mouse protein IP'd with Ikka), Fig. 4b WB (mouse hepatocyte infected with LacZAdv or dnIkk2Adv IP'd with anti-NEMO), Fig. 4e 2. Ikkb [IMG-129A] WB (mouse whole liver pr
• Shishodia S, Aggarwal BB. Guggulsterone inhibits NF-kappaB and IkappaBalpha kinase activation, suppresses expression of anti-apoptotic gene products, and enhances apoptosis. J Biol Chem. 2004-11-05 [PMID: 15322087] (WB, Human)
• Shishodia S, Koul D, Aggarwal BB. Cyclooxygenase (COX)-2 inhibitor celecoxib abrogates TNF-induced NF-kappa B activation through inhibition of activation of I kappa B alpha kinase and Akt in human non-small cell lung carcinoma: correlation with suppression of COX-2 synthesis. J Immunol. 2004-08-01 [PMID: 15265936] (WB)
• Takada Y, Fang X, Jamaluddin MS et al. Genetic deletion of glycogen synthase kinase-3beta abrogates activation of IkappaBalpha kinase, JNK, Akt, and p44/p42 MAPK but potentiates apoptosis induced by tumor necrosis factor. J Biol Chem. 2004-09-17 [PMID: 15252041] (WB)
• Takada Y, Khuri FR, Aggarwal BB. Protein farnesyltransferase inhibitor (SCH 66336) abolishes NF-kappaB activation induced by various carcinogens and inflammatory stimuli leading to suppression of NF-kappaB-regulated gene expression and up-regulation of apoptosis. J Biol Chem. 2004-06-18 [PMID: 15090542] (WB)
• Takada Y, Singh S, Aggarwal BB. Identification of a p65 peptide that selectively inhibits NF-kappa B activation induced by various inflammatory stimuli and its role in down-regulation of NF-kappaB-mediated gene expression and up-regulation of apoptosis. J Biol Chem. 2004-04-09 [PMID: 14711835] (WB, Human)
• Takada Y, Aggarwal BB. Flavopiridol inhibits NF-kappaB activation induced by various carcinogens and inflammatory agents through inhibition of IkappaBalpha kinase and p65 phosphorylation: abrogation of cyclin D1, cyclooxygenase-2, and matrix metalloprotease-9. J Biol Chem. 2004-02-06 [PMID: 14630924] (WB)
• Leis H, Page A, Ramirez A et al. Glucocorticoid Receptor Counteracts Tumorigenic Activity of Akt in Skin through Interference with the Phosphatidylinositol 3-Kinase Signaling Pathway. Mol Endocrinol. 2004-02-01 [PMID: 14615607] (WB, Mouse)
  
  Details:
  WB (nontransgenic and K5-GR mouse dorsal skin), Fig. 2a.
• Majumdar S, Aggarwal BB. Adenosine suppresses activation of nuclear factor-kappaB selectively induced by tumor necrosis factor in different cell types. Oncogene. 2003-02-27 [PMID: 12606947] (WB)
• Santos M, Perez P, Segrelles C et al. Impaired NF-kappa B activation and increased production of tumor necrosis factor alpha in transgenic mice expressing keratin K10 in the basal layer of the epidermis. J Biol Chem. 2003-04-11 [PMID: 12566451] (WB, Mouse)
  
  Details:
  WB (bk5hk10 transgenic mice epidermis), Fig. 2A' WB (PB keratinocytes infected with retrovirus coding for DN Akt, wt Akt, or empty retroviral backbone), Fig. 3
• Ashikawa K, Majumdar S, Banerjee S et al. Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation. J Immunol. 2002-12-01 [PMID: 12444159] (WB, IP)
• Anto RJ, Mukhopadhyay A, Shishodia S et al. Cigarette smoke condensate activates nuclear transcription factor-kappaB through phosphorylation and degradation of IkappaB(alpha): correlation with induction of cyclooxygenase-2. Carcinogenesis. 2002-09-01 [PMID: 12189195] (WB)
• Majumdar S, Lamothe B, Aggarwal BB. Thalidomide suppresses NF-kappa B activation induced by TNF and H2O2, but not that activated by ceramide, lipopolysaccharides, or phorbol ester. J Immunol. 2002-03-15 [PMID: 11884428] (IP)
• Majumdar S, Aggarwal BB. Methotrexate suppresses NF-kappaB activation through inhibition of IkappaBalpha phosphorylation and degradation. J Immunol. 2001-09-01 [PMID: 11509639] (WB)
• Miller BS, Zandi E. Complete reconstitution of human IkappaB kinase (IKK) complex in yeast. Assessment of its stoichiometry and the role of IKKgamma on the complex activity in the absence of stimulation. J Biol Chem. 2001-09-28 [PMID: 11470787] (WB)
• Manna SK, Mukhopadhyay A, Aggarwal BB. Leflunomide suppresses TNF-induced cellular responses: effects on NF-kappa B, activator protein-1, c-Jun N-terminal protein kinase, and apoptosis. J Immunol. 2000-11-15 [PMID: 11067959] (WB)

Reviews for IKK beta Antibody (NB100-56509) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Michael Nowak ICC Human 01/26/2022

Summary

• Application: Immunocytochemistry
• Sample Tested: HeLa cells
• Species: Human
• Lot: 10AG2

Comments

• Comments: Dilution 5 ug/ml

reviewed by: Shinobu Miyazaki WB Mouse 05/06/2014

Summary

• Application: Western Blot
• Sample Tested: Cell lysate
• Species: Mouse
• Lot: AB101805A-12

Bioinformatics

• Gene Symbol: IKBKB
• Entrez:
  • 3551(Human)
• Uniprot:
  • O14920()