IKK beta Antibody (10AG2)

Images

 
Western Blot: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of A) human Daudi, B) HeLa, and C) mouse NIH3T3 lysate probed with IKKbeta antibody at 2 ug/ml.
Immunohistochemistry-Paraffin: IKK beta Antibody (10AG2) [NB100-56509] - Human gall bladder probed with IKKbeta antibody at 5 ug/ml. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is ...read more
Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of PE conjugate of NB100-56509. An intracellular stain was performed on Jurkat cells with IKK Beta antibody (10AG2) NB100-56509PE (blue) and a matched ...read more
Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Intracellular staining of HEK293 cells with 0.5 ug of IKKbeta antibody (red) and 0.5 ug of isotype control antibody (20109, green). Intracellular flow kit was ...read more
Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56509. Staining of IKKbeta in HEK 293 cells using 0.1 ug of Alexa Fluor 488-conjugated antibody. Green ...read more
Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis using Alexa Fluor (R) 647 conjugate of NB100-56509. An intracellular stain was performed on HeLa cells with IKK beta antibody (10AG2) NB100-56509 (blue) ...read more
Simple Western: IKK beta Antibody (10AG2) [NB100-56509] - Lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Clone
10AG2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

IKK beta Antibody (10AG2) Summary

Immunogen
A full-length human IKKb recombinant protein was used to immunize mice (NP_001547).
Specificity
An approx. 87 kDa band should be observed.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IKBKB
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 - 4 ug/ml
  • Simple Western 1:12.5
  • Flow Cytometry 0.25 - 0.5 ug/ 10^6 cells
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • CyTOF-ready
Application Notes
Useful in IHC-P See Page et al. Use in ICC/IF reported in scientific literature (PMID 24825920)

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
Theoretical MW
86.564 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
IKK beta Lysate (NBP2-64726)
Reviewed Applications
Read 1 Review rated 4
using
NB100-56509 in the following applications:

Publications
Read Publications using
NB100-56509 in the following applications:

  • 2 publications
  • 1 publication
  • IP
    5 publications
  • WB
    37 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for IKK beta Antibody (10AG2)

  • EC 2.7.11
  • EC 2.7.11.10
  • I-kappa-B kinase 2
  • I-kappa-B-kinase beta
  • IkBKB
  • IKK beta
  • IKK2
  • IKK2FLJ38368
  • IKK-B
  • IKK-betaFLJ36218
  • IKKBFLJ40509
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
  • inhibitor of nuclear factor kappa-B kinase subunit beta
  • MGC131801
  • NFKBIKB
  • NFKBIKBFLJ33771
  • Nuclear factor NF-kappa-B inhibitor kinase beta

Background

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli. Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IKK beta Antibody (NB100-56509)(53)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC-P, IP, WB.


Filter By Application
ICC/IF
(2)
IHC-P
(1)
IP
(5)
WB
(37)
All Applications
Filter By Species
Human
(13)
Mouse
(13)
All Species
Showing Publications 1 - 10 of 53. Show All 53 Publications.
Publications using NB100-56509 Applications Species
De S, Karim F, Kiessu E et al. Mechanism of dysfunction of human variants of the IRAK4 kinase and a role for its kinase activity in interleukin-1 receptor signaling J. Biol. Chem. Aug 16 2018 [PMID: 30115681] (WB, Human) WB Human
Abdi K, Lai CH, Paez-Gonzalez P et al. Uncovering inherent cellular plasticity of multiciliated ependyma leading to ventricular wall transformation and hydrocephalus. Nat Commun Apr 25 2018 [PMID: 29695808] (Mouse) Mouse
Kirkley KS, Walton KD, Duncan C, Tjalkens RB. Spontaneous Development of Cutaneous Squamous Cell Carcinoma in Mice with Cell-specific Deletion of Inhibitor of kB Kinase 2 Comp. Med. 2017 Oct 01 [PMID: 28935002] (IHC-P, Mouse) IHC-P Mouse
Cleary MM, Mansoor A, Settelmeyer T et al. NFkB signaling in alveolar rhabdomyosarcoma Dis Model Mech 2017 Sep 01 [PMID: 28883017] (WB, Mouse) WB Mouse
Liu J, Ibi D, Taniguchi K et al. Inflammation Improves Glucose Homeostasis through IKKbeta-XBP1s Interaction. Cell. Nov 3 2016 [PMID: 27814504] (Human) Human
Buhrmann C, Busch F, Shayan P, Shakibaei M. Sirtuin-1 (SIRT1) Is Required for Promoting Chondrogenic Differentiation of Mesenchymal Stem Cells. J. Biol. Chem. 2014 Jun 24 [PMID: 24962570] (IP, WB, Human)

Details:
IP & WB: Fig 5C, Primary chondrocytes and mesenchymal stem cells. The IKK beta mAb immunoprecipitated an active immune complex as shown by an immune complex assay (Fig 5C).
IP, WB Human
Harikumar KB, Sung B, Pandey MK et al. Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway Mol Pharmacol

Details:
This publication used the Alexa Fluor (R) 488 conjugated form of this antibody (Cat# NBP2-24962).
Das U, Manna K, Sinha M et al. Role of ferulic Acid in the amelioration of ionizing radiation induced inflammation: a murine model. PLoS ONE. 2014 May 23 [PMID: 24854039] (WB, Mouse)

Details:
Liver, Fig 7.
WB Mouse
Paul S, Traver MK, Kashyap AK et al. T Cell Receptor Signals to NF-kB Are Transmitted by a Cytosolic p62-Bcl10-Malt1-Ikk Signalosome. Sci Signal. 2014 May 14 [PMID: 24825920] (ICC/IF, Mouse)

Details:
D10 mouse T-helper cells, Fig S1B
ICC/IF Mouse
Breuer R, Becker MS, Brechmann M et al. The PP2A phosphatase regulatory subunit B56-gamma mediates suppression of T-cell receptor (TCR)-induced nuclear factor-kappa B (NF-kB) activity. J. Biol. Chem. 2014 Apr 09 [PMID: 24719332] (WB, Human)

Details:
Jurkat, Fig 3.
WB Human
Show All 53 Publications.

Review for IKK beta Antibody (NB100-56509) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-56509:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot IKK beta NB100-56509
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reviewed by:
Anonymous
WB Mouse 05/06/2014
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Summary

ApplicationWestern Blot
Sample TestedCell lysate
SpeciesMouse
LotAB101805A-12

Blocking

Blocking Details5% Milk / 1x TBST 2 hours @ RT

Primary Anitbody

Dilution Ratio1:1000 1 hour @ RT 5% Milk / 1x TBST

Secondary Antibody

Secondary DescriptionGoat anti-Mouse Ig G HRP
Secondary Manufacturer Cat#Thermo 31430
Secondary Concentration1:10000

Details

Detection NotesECL, film, less than 5 min

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IKK beta Antibody (NB100-56509) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56509AF405
Alexa Fluor 488 Labeled NB100-56509AF488
Alexa Fluor 647 Labeled NB100-56509AF647
Alexa Fluor 700 Labeled NB100-56509AF700
Allophycocyanin Labeled NB100-56509APC
DyLight 350 Labeled NB100-56509UV
DyLight 405 Labeled NB100-56509V
DyLight 488 Labeled NB100-56509G
DyLight 550 Labeled NB100-56509R
DyLight 650 Labeled NB100-56509C
DyLight 680 Labeled NB100-56509FR
DyLight 755 Labeled NB100-56509IR
HRP Labeled NB100-56509H
PE Labeled NB100-56509PE
PerCP Labeled NB100-56509PCP

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Bioinformatics Tool for IKK beta Antibody (NB100-56509)

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Blogs on IKK beta.

Regulating Immune Response Pathways with IKK beta
IKK beta, also known as IKK2, activates the NFkB complex by phosphorylating the NFkB inhibitor, IkBa. Several transcript variants, some protein-coding and some not, have been found for IKKB. The Nuclear Factor-kappa B (NF-kB) family of transcription f...  Read full blog post.

IKK alpha: Roles in Development, B-cell Survival and ESC Differentiation
Inhibitor of nuclear factor kappa-B kinase subunit alpha (IKK1 alpha) is a serine/threonine kinase that forms a complex with IKK beta and NEMO. It plays an essential role in embryonic skin development. Mice with low levels of IKKa show an increase in...  Read full blog post.

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
05/06/2014
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol IKBKB
Entrez
Uniprot