CD45 Antibody (30-F11)

Images

 
Flow Cytometry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of CD45 (30F11) in freshly isolated mouse (Balb C) splenocytes (1x10^6) were stained with [NB100-77417] at 1:1000 dilution and detected with FITC ...read more
Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Analysis of FFPE section of mouse spleen using rat monoclonal CD45 antibody (30-F11) [NB100-77417] at 1:50 dilution. The antibody generated an ...read more
Immunohistochemistry-Frozen: CD45 Antibody (30-F11) [NB100-77417] - Analysis of a mouse spleen cryosection using CD45 antibody (30-F11) [NB100-77417] at 1:50 dilution. The signal was detected using goat anti-rat Alexa ...read more
Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Staining of a FFPE mouse liver tissue section using 1:100 dilution of CD45 antibody (30-F11) [NB100-77417]. Signal was detected using AF488 ...read more
Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of a FFPE embedded tissue section of mouse liver using 1:100 dilution of CD45 antibody (30-F11) [NB100-77417]. The signal was developed using AEC ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, In vivo, IP, CyTOF-ready
Clone
30-F11
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CD45 Antibody (30-F11) Summary

Immunogen
This CD45 Antibody (30-F11) was prepared from Mouse thymus or spleen.
Localization
Transmembrane Protein with Extracellular, Helical and Cytoplasmic Domains
Specificity
CD45 Antibody (YKIX716.13) detects mouse CD45. Recognizes all CD45 isoforms as well as the known mouse CD45 alloantigens (CD45-1/Ly 5a and CD45-2/Ly 5b)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
PTPRC
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • In vitro assay
  • In vivo assay
  • CyTOF-ready
Application Notes
Each lot of this CD45 antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.25 microgram per one million cells in 100 microliter volume. Immunohistochemistry, it is recommended that the reagent be titrated for optimal performance for each application. NB100-77417 may also be used for IP, Western blot and IHC (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections). IF data from customer review. Use in vivo reported in multiple pieces scientific literature. Use in functional reported in scientific literature (PMID: 7743522). Use in In vitro reported in scientific literature (PMID: 1974916). This antibody is CyTOF ready.
Theoretical MW
145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 4 Reviews rated 4.8
using
NB100-77417 in the following applications:

Publications
Read Publications using
NB100-77417 in the following applications:

Reactivity Notes

CD45 Antibody (30-F11) shows reactivity with Human as reported in scientific literature (PMID: 29367423).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD45 Antibody (30-F11)

  • B220
  • CD_antigen: CD45
  • CD45 antigen
  • CD45
  • CD45R
  • EC 3.1.3.48
  • EC:3.1.3.48
  • GP180
  • LCA
  • L-CA
  • Leukocyte common antigen
  • LY5
  • protein tyrosine phosphatase, receptor type, c polypeptide
  • PTPRC
  • receptor-type tyrosine-protein phosphatase C
  • T200 Glycoprotein
  • T200 leukocyte common antigen
  • T200

Background

CD45, also known as leukocyte common antigen (LCA), T200, or Ly5, is a member C of the class 1 receptor-like protein tyrosine phosphatase (PTPRC) family (1, 2). It is a transmembrane glycoprotein which, due to alternative splicing, has a multiple isoforms with a theoretical molecular weight ranging from 180 - 220 kDa (1, 3-5). Human CD45 is synthesized as a 1281 amino acid sequence consisting of an alternatively spliced extracellular receptor-like region, a cysteine-rich domain, fibronectin-like III repeats, a transmembrane segment, and a cytoplasmic region with tandem protein tyrosine phosphatase (PTP) domains: the membrane proximal domain (D1) and the membrane distal domain (D2) (3, 5). CD45 is expressed on all nucleated hematopoietic cells and their precursors, except mature red blood cells, and is one of the most abundantly-expressed cell-surface glycoproteins, comprising approximately 10% of surface proteins in lymphocytes (3). Functionally, CD45 is essential for development and activation of T-cells and B-cells (1-5). More specifically, CD45 positively regulates antigen receptor signaling and Src-family member kinase activity (1, 3). There are many ways to regulate CD45 phosphatase activity including ligand binding, dimerization, protein interactions, cellular localization, and covalent modifications (3, 6). Ligands for CD45 include pUL11, a transmembrane protein of the cytomegalovirus RL11 (CMV RL11) family, and placental protein 14 (PP14), both of which exclusively bind CD45, and various lectins including CD22, galectin-1, galectin-3, macrophage mannose receptor (MR), and macrophage galactose-type lectin (MGL) (6).

Given its role in immune cell development and activation, CD45 has also been linked to a variety of diseases. The importance of CD45 in immunity has been revealed in human and mouse studies where CD45-deficiency leads to a severe-combined immunodeficiency (SCID) phenotype (2, 3, 6). A CD45-knockout mice study revealed inhibited thymocyte production and poor B-cell response, whereas CD45 activation in mice causes lymphoproliferation and autoantibody production (3). CD45 variants have been associated with altered immune function and autoimmune disorders including multiple sclerosis, systemic lupus erythematosus (SLE), and rheumatoid arthritis (6). Furthermore, altered CD45 expression has been implicated in oncological conditions including chronic lymphatic leukemia, acute lymphatic leukemia, Hodgkin lymphoma, multiple myeloma, and diffuse large B-cell lymphoma (6). Considering its role in autoimmune disorders, immunodeficiency and cancer, CD45 is an ideal therapeutic target (3, 6). The main approaches to control CD45 function is through either selective inhibitors or anti-CD45 antibodies (3).

Alternative names for CD45 includes B220, CD antigen: CD45, CD45 antigen, CD45R, EC 3.1.3.48, GP180, LCA, Leukocyte common antigen, LY5, protein tyrosine phosphatase receptor type c polypeptide, PTPRC, receptor-type tyrosine-protein phosphatase C, T200 Glycoprotein, and T200.

References

1. Trowbridge, I. S., & Thomas, M. L. (1994). CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development. Annual review of immunology. https://doi.org/10.1146/annurev.iy.12.040194.000505

2. Andersen, J. N., Jansen, P. G., Echwald, S. M., Mortensen, O. H., Fukada, T., Del Vecchio, R., Tonks, N. K., & Moller, N. P. (2004). A genomic perspective on protein tyrosine phosphatases: gene structure, pseudogenes, and genetic disease linkage. FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

3. Hermiston, M. L., Xu, Z., & Weiss, A. (2003). CD45: a critical regulator of signaling thresholds in immune cells. Annual review of immunology. https://doi.org/10.1146/annurev.immunol.21.120601.140946

4. Tonks, N. K., Diltz, C. D., & Fischer, E. H. (1990). CD45, an integral membrane protein tyrosine phosphatase. Characterization of enzyme activity. The Journal of biological chemistry.

5. Nam, H. J., Poy, F., Saito, H., & Frederick, C. A. (2005). Structural basis for the function and regulation of the receptor protein tyrosine phosphatase CD45. The Journal of experimental medicine. https://doi.org/10.1084/jem.20041890

6. Rheinlander, A., Schraven, B., & Bommhardt, U. (2018). CD45 in human physiology and clinical medicine. Immunology letters. https://doi.org/10.1016/j.imlet.2018.01.009

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD45 Antibody (NB100-77417)(24)

We have publications tested in 2 confirmed species: Mouse, Mouse .

We have publications tested in 9 applications: FLOW, Flow, Func, ICC/IF, IHC, IHC-P, In vitro, In vivo, WB.


Filter By Application
FLOW
(3)
Flow
(1)
Func
(2)
ICC/IF
(1)
IHC
(7)
IHC-P
(1)
In vitro
(1)
In vivo
(3)
WB
(2)
All Applications
Filter By Species
Mouse
(17)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NB100-77417 Applications Species
Jakubowska MA, Pyka J, Michalczyk-Wetula D et al. Electron paramagnetic resonance spectroscopy reveals alterations in the redox state of endogenous copper and iron complexes in photodynamic stress-induced ischemic mouse liver Redox Biol May 12 2020 [PMID: 32464500] (IHC, Mouse) IHC Mouse
Simanjuntak Y, Ko HY, Lee YL et al. Preventive effects of folic acid on Zika virus-associated poor pregnancy outcomes in immunocompromised mice PLoS Pathog. May 1 2020 [PMID: 32392268] (IHC, Mouse) IHC Mouse
Chen BY, Ghezzi C, Villegas B et al. 18F-FAC PET visualizes brain-infiltrating leukocytes in a mouse model of multiple sclerosis J. Nucl. Med. Oct 25 2019 [PMID: 31653711] (IHC) IHC
Chang CS, Liao CC, Liou AT et al. Enterovirus 71 targets the cardiopulmonary system in a robust oral infection mouse model Sci Rep Jul 31 2019 [PMID: 31366973] (IHC, Mouse) IHC Mouse
Sharifpanah F, Ali EH, Wartenberg M, Sauer H. The milk thistle (Silybum marianum) compound Silibinin stimulates leukopoiesis from mouse embryonic stem cells Phytother Res Dec 11 2018 [PMID: 30548344] (WB, Mouse) WB Mouse
Kramann R, Machado F, Wu H et al. Parabiosis and single-cell RNA sequencing reveal a limited contribution of monocytes to myofibroblasts in kidney fibrosis. JCI Insight 2018 May 3 [PMID: 29720573]
Samanta D, Park Y, Ni X et al. Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells Proc. Natl. Acad. Sci. U.S.A. 2018 Jan 24 [PMID: 29367423] (FLOW, Mouse)

Details:
This citation used the Alexa Fluor 488 form of this antbody
FLOW Mouse
Caballero S, Kent DL, Sengupta N et al. Bone Marrow-Derived Cell Recruitment to the Neurosensory Retina and Retinal Pigment Epithelial Cell Layer Following Subthreshold Retinal Phototherapy. Invest. Ophthalmol. Vis. Sci. 2017 Oct 01 [PMID: 29049716] (IHC, Mouse) IHC Mouse
Khoriaty R, Vogel N, Hoenerhoff MJ et al. SEC23B is required for pancreatic acinar cell function in adult mice. Mol. Biol. Cell May 24 2017 [PMID: 28539403] (IHC, Mouse ) IHC Mouse
Ambrosi TH, Scialdone A, Graja A et al. Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration. Cell Stem Cell. Mar 13 2017 [PMID: 28330582] (Flow, Mouse) Flow Mouse
Show All 24 Publications.

Reviews for CD45 Antibody (NB100-77417) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Mouse, Rat.

Reviews using NB100-77417:
Filter by Applications
IHC-P
(1)
IF
(2)
IHC
(1)
All Applications
Filter by Species
Mouse
(3)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CD45 NB100-77417
Enlarge
5
reviewed by:
Anonymous
IHC-P Mouse 09/15/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse spleen cells
SpeciesMouse
LotB-13

Comments

CommentsNo permeabilization
Antigen retrieval: Citrate buffer, 20 minutes, 90C
1st ab dilution: 1:100

  5
reviewed by:
Anonymous
IF Rat 11/24/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse Spleen
SpeciesRat

Blocking

Blocking Details5% goat serum in PBS for 30 min at RT

Primary Anitbody

Dilution Ratio1:50 in blocking buffer o/n at 4°C

Secondary Antibody

Secondary DescriptionGoat anti-rat Alexa fluor 488
Secondary Concentration1:500

Details

Detection NotesFluorescence microscope. Exposure time: 200 ms. Negative control: secondary antibody only
Fixation DetailsAcetone fixation for 10 min at -20°C, No permeabilization
Wash Description3 x PBS for 10 min at RT
  5
reviewed by:
Anonymous
IHC Mouse 10/02/2014
View

Summary

ApplicationImmunohistochemistry
Sample Testedmouse liver
SpeciesMouse

Blocking

Blocking DetailsBlocking with 5% goeat serum in PBS for 1 hour at RT

Primary Anitbody

Dilution Ratio1:100

Secondary Antibody

Secondary Descriptiongoat anti-rat
Secondary Concentration1:500

Details

Detection NotesAEC reagent
Fixation DetailsFixation with formalin and paraffind embedding. Antigen retrieval: boiling for 10 min in citrate buffer
Wash DescriptionPBS
Immunofluorescence CD45 NB100-77417
Enlarge
4
reviewed by:
Aurora De Ponti
IF Mouse 09/05/2014
View

Summary

ApplicationImmunofluorescence
Sample Testedmouse liver
SpeciesMouse
LotB144981

Blocking

Blocking DetailsDonkey serum 5% in PBS. 1h at RT

Primary Anitbody

Dilution Ratio1:100

Secondary Antibody

Secondary DescriptionDonkey anti rat 488
Secondary Concentration1:500

Details

Detection NotesMagnification: 40x.
Fixation DetailsParaffin section. Antigen retrieva: Citrate buffer
Wash Description20 min in PBS

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD45 Antibody (NB100-77417). (Showing 1 - 4 of 4 FAQs).

  1. We would like to order a CD45 antibody to stain immune cells that were isolated from a ligated sciatic nerve of mice with double immunofluorescence (using PFA-fixed tissue on slides). Which of the following rat monoclonal CD45 antibodies would you recommend: 30-F11, IBL-3/16 or 5C16?
    • I would recommend clone 30-F11. As clone IBL-3/16 has not yet been validated for IHC-P application, we would not be able to guarantee its workability on PFA-fixed tissue. Although both other clones i.e. 30-F11 and 5C16, would be good for your samples, CD45 30-F11 # NB100-77417 is a well known clone that offers more flexibility around protocol because of availability of its conjugated forms. It would be advantageous to use a conjugated primary as you are planning for double-immunostaining procedure.
  2. For use in Western Blot with CD45 antibodies, what molecular weight of the band should I expect to see?
    • The theoretical molecular weight for most of our CD45 antibodies is 147 kDa based off the first isoform. Any variation on 147 is due to the immunogen being from a different species and the protein being a slightly different size. CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated.
  3. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
    • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product, allowing the tested application/species to fall under our 100% guarantee. Please check out our Innovator's Reward Program if you decide to test a primary antibody with a species or application that is not currently listed. Please note that the Innovator's Reward Program only applies to our primary antibodies.
  4. Is CD45 a good target for use in Neurodegeneration studies?
    • Yes, here are the approved research areas we have listed for our CD45 products: Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Glia Markers, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, Mast Cell Markers, Mesenchymal Stem Cell Markers, Microglia Markers, Myeloid Cell Markers, Myeloid-derived Suppressor, Neurodegeneration, Neuroscience, Signal Transduction, Stem Cell Markers, Growth and Development.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-77417AF350
Alexa Fluor 405 NB100-77417AF405
Alexa Fluor 488 NB100-77417AF488
Alexa Fluor 532 NB100-77417AF532
Alexa Fluor 594 NB100-77417AF594
Alexa Fluor 647 NB100-77417AF647
Alexa Fluor 700 NB100-77417AF700
Alexa Fluor 750 NB100-77417AF750
Allophycocyanin NB100-77417APC
DyLight 350 NB100-77417UV
DyLight 405 NB100-77417V
DyLight 488 NB100-77417G
DyLight 550 NB100-77417R
DyLight 594 NB100-77417DL594
DyLight 650 NB100-77417C
DyLight 680 NB100-77417FR
DyLight 755 NB100-77417IR
FITC NB100-77417F
HRP NB100-77417H
Janelia Fluor 549 NB100-77417JF549
Janelia Fluor 646 NB100-77417JF646
PE NB100-77417PE
PerCP NB100-77417PCP

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Bioinformatics Tool for CD45 Antibody (NB100-77417)

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Blogs on CD45.

Gut-brain axis: microbiota influence behavior and mental well-being
By Jennifer Sokolowski, MD, PhD.Gut-microbiome interactionsOn and in our bodies, microbes outnumber our cells by about ten-to-one. Studies have revealed that the microbiome influences neurogenesis, cognition, and...  Read full blog post.

How to identify B cell subsets using flow cytometry
By Victoria OsinskiUsing flow cytometry to identify B cell subsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified a...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

CD45 - Much more than just a housekeeping protein
CD45, also known as T200 or the Leukocyte Common Antigen (LCA), is encoded by the Protein Tyrosine Phosphatase Receptor type C (PTPRC) gene. The protein is expressed exclusively on cells of the haematopoietic system, and is one of the most abundant le...  Read full blog post.

CD45 Isoforms: Hematopoietic Differentiation, Cancer and Alzheimer's
CD45, also known as protein tyrosine phosphatase, receptor type, C (PTPRC), was originally known as common leukocyte antigen and is a signal transducer involved in many physiological processes such as growth and differentiation, cancer transformation,...  Read full blog post.

Vimentin Antibodies in Rheumatoid Arthritis & Cataracts Research
Vimentin is a 57kDa type III intermediate filament (IF) protein that is the major cytoskeletal component of mesenchymal cells and the first to be expressed during cell differentiation. It plays a significant role in supporting and anchoring the positi...  Read full blog post.

Antibodies In The Differential Diagnosis Of Undifferentiated Tumors
Immunohistochemical testing using antibody panels has a valuable role in cancer diagnosis. Some tumors, especially more malignant ones, tend to lose the appearance of the tissue of origin. However, knowing the tissue of origin assists the physician in...  Read full blog post.

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Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

Anonymous
09/15/2017
Application: IHC-P
Species: Mouse

 
Anonymous
11/24/2015
Application: IF
Species: Rat

 
Anonymous
10/02/2014
Application: IHC
Species: Mouse

Bioinformatics

Gene Symbol PTPRC
Entrez
Uniprot