CD45 Antibody (30-F11)


Immunocytochemistry/ Immunofluorescence: CD45 Antibody (30-F11) [NB100-77417] - IF on mouse liver. Image from verified customer review.
Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - IHC analysis of formalin fixed and paraffin embedded section of mouse spleen using rat monoclonal CD45 antibody (clone 30-F11) at 1:50 dilution. The more
Flow Cytometry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of CD45 (30F11) in freshly isolated mouse (Balb C) splenocytes (1x10^6) were stained with CD45 antibody (NB100-77417) at 1:1000 dilution and detected with more
Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - Mouse liver tissue was stained with anti-CD45 antibody.
Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - IHC analysis of formalin fixed and paraffin embedded section of mouse spleen using rat monoclonal CD45 antibody (clone 30-F11) at 1:50 dilution. The more
Immunofluorescence: CD45 Antibody (30-F11) [NB100-77417] - Mouse spleen cryo section stained with anti-Cd45 antibody. Image from verified customer review.

Product Details

Reactivity MuSpecies Glossary
Applications WB, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, In vivo, IP
1.0 mg/ml

CD45 Antibody (30-F11) Summary

Mouse thymus or spleen
Transmembrane Protein with Extracellular, Helical and Cytoplasmic Domains
CD45 (30-F11)
IgG2b Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Protein G purified


  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • In vivo assay
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.25 microgram per one million cells in 100 microliter volume. Immunohistochemistry, It is recommended that the reagent be titrated for optimal performance for each application. NB100-77417 may also be used for immunoprecipitation, Western blot and immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections). IF data from customer review. Use in functional, and in vivo reported in multiple pieces scientific literature.
Reviewed Applications
Read 3 Reviews rated 4.7
NB100-77417 in the following applications:

Read Publications using
NB100-77417 in the following applications:

Alternate Names for CD45 Antibody (30-F11)

  • B220
  • CD45 antigen
  • CD45
  • CD45R
  • EC
  • LCA
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide


CD45 is a 180-240 kD glycoprotein, also known as the leukocyte common antigen (LCA), T200 or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell, as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD45 Antibody (NB100-77417)(14)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 8 applications: FLOW, Func, ICC/IF, IHC, IHC-P, In vitro, In vivo, WB.

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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB100-77417 Applications Species
Mendes VI, Bartholomeusz GA, Ayres M et al. Synthesis and cytotoxic activity of novel A-ring cleaved ursolic acid derivatives in human non-small cell lung cancer cells Eur J Med Chem 2016 Jul 22 [PMID:27484517] (IHC, Mouse) IHC Mouse
Celik Mo, Labuz D, Henning K et al. Leukocyte opioid receptors mediate analgesia via Ca(2+)-regulated release of opioid peptides. Brain Behav. Immun. Apr 30 2016 12:00AM [PMID:27139929] (ICC/IF, Mouse) ICC/IF Mouse
De Ponti A, Wiechert L, Stojanovic A et al. Chronic liver inflammation and hepatocellular carcinogenesis are independent of S100A9. Int. J. Cancer. 2014 Oct 21 [PMID:25331529] (IHC-P, Mouse) IHC-P Mouse
Tsuboi S, Fukuda M. Overexpression of branched O-linked oligosaccharides on T cell surface glycoproteins impairs humoral immune responses in transgenic mice.. J Biol Chem. 1998 Nov 13 [PMID:9804842] (WB) WB
Keller G, Wall C, Fong AZ, Hawley TS et al. Overexpression of HOX11 leads to the immortalization of embryonic precursors with both primitive and definitive hematopoietic potential.. Blood. 1998 Aug 1 [PMID:9680355] (FLOW) FLOW
Maroun CR, Julius M. Distinct involvement of CD45 in antigen receptor signalling in CD4+ and CD8+ primary T cells.. Eur J Immunol. 1994 Apr 1 [PMID:7908636]
Hanaoka K, Fujita N, Lee SH et al. Involvement of CD45 in adhesion and suppression of apoptosis of mouse malignant T-lymphoma cells.. Cancer Res. 1995 May 15 [PMID:7743522] (Func, Mouse) Func Mouse
Seaman WE, Talal N, Herzenberg LA et al. Surface antigens on mouse natural killer cells: use of monoclonal antibodies to inhibit or to enrich cytotoxic activity.. J Immunol. 1981 Sep 1 [PMID:6790624]
Ralph SJ, Berridge MV et al. Expression of antigens of the 'T200' family of glycoproteins on hemopoietic stem cells: evidence that thymocyte cell lineage antigens are represented on 'T200'.. J Immunol. 1984 May 1 [PMID:6425407] (Func, Mouse) Func Mouse
Seaman WE, Wofsy D, Greenspan JS, Ledbetter JA. Treatment of autoimmune MRL/Ipr mice with monoclonal antibody to Thy-1.2: a single injection has sustained effects on lymphoproliferation and renal disease.. J Immunol. 1983 Apr 1 [PMID:6187819] (In vivo, Mouse) In vivo Mouse
Show All 14 Publications.

Reviews for CD45 Antibody (NB100-77417) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Mouse, Rat.
We have 3 reviews tested in 2 application: IHC, IF.

Reviews using NB100-77417:
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Images Ratings Applications Species Date Details
 CD45 NB100-77417
reviewed by:
Mouse 11/24/2015


Sample TestedMouse Spleen


Blocking Details5% goat serum in PBS for 30 min at RT

Primary Anitbody

Dilution Ratio1:50 in blocking buffer o/n at 4°C

Secondary Antibody

Secondary DescriptionGoat anti-rat Alexa fluor 488
Secondary Concentration1:500


Detection NotesFluorescence microscope. Exposure time: 200 ms. Negative control: secondary antibody only
Fixation DetailsAcetone fixation for 10 min at -20°C, No permeabilization
Wash Description3 x PBS for 10 min at RT
Immunohistochemistry CD45 NB100-77417
reviewed by:
IHC Rat 10/02/2014


Sample Testedmouse liver


Blocking DetailsBlocking with 5% goeat serum in PBS for 1 hour at RT

Primary Anitbody

Dilution Ratio1:100

Secondary Antibody

Secondary Descriptiongoat anti-rat
Secondary Concentration1:500


Detection NotesAEC reagent
Fixation DetailsFixation with formalin and paraffind embedding. Antigen retrieval: boiling for 10 min in citrate buffer
Wash DescriptionPBS
Immunofluorescence CD45 NB100-77417
reviewed by:
IF Mouse 09/05/2014


Sample Testedmouse liver


Blocking DetailsDonkey serum 5% in PBS. 1h at RT

Primary Anitbody

Dilution Ratio1:100

Secondary Antibody

Secondary DescriptionDonkey anti rat 488
Secondary Concentration1:500


Detection NotesMagnification: 40x.
Fixation DetailsParaffin section. Antigen retrieva: Citrate buffer
Wash Description20 min in PBS

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD45 Antibody (NB100-77417). (Showing 1 - 2 of 2 FAQs).

  1. We are interested in CD45 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • I would recommend one of these CD45 antibodies to you, as these have all been validated for use with dog/canine samples.
  2. We would like to order a CD45 antibody to stain immune cells that were isolated from a ligated sciatic nerve of mice with double immunofluorescence (using PFA-fixed tissue on slides). Which of the following rat monoclonal CD45 antibodies would you recommend: 30-F11, IBL-3/16 or 5C16?
    • I would recommend clone 30-F11. As clone IBL-3/16 has not yet been validated for IHC-P application, we would not be able to guarantee its workability on PFA-fixed tissue. Although both other clones i.e. 30-F11 and 5C16, would be good for your samples, CD45 30-F11 # NB100-77417 is a well known clone that offers more flexibility around protocol because of availability of its conjugated forms. It would be advantageous to use a conjugated primary as you are planning for double-immunostaining procedure.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-77417AF405
Alexa Fluor (R) 488 Labeled NB100-77417AF488
Alexa Fluor (R) 647 Labeled NB100-77417AF647
Alexa Fluor (R) 700 Labeled NB100-77417AF700
Allophycocyanin Labeled NB100-77417APC
DyLight 350 Labeled NB100-77417UV
DyLight 405 Labeled NB100-77417V
DyLight 405LS Labeled NB100-77417V3
DyLight 488 Labeled NB100-77417G
DyLight 550 Labeled NB100-77417R
DyLight 650 Labeled NB100-77417C
DyLight 680 Labeled NB100-77417FR
DyLight 755 Labeled NB100-77417IR
PE Labeled NB100-77417PE
PerCP Labeled NB100-77417PCP

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Blogs on CD45.

CD45 - Much more than just a housekeeping protein
CD45, also known as T200 or the Leukocyte Common Antigen (LCA), is encoded by the Protein Tyrosine Phosphatase Receptor type C (PTPRC) gene. The protein is expressed exclusively on cells of the haematopoietic system, and is one of the most abundant le...  Read full blog post.

CD45 Isoforms: Hematopoietic Differentiation, Cancer and Alzheimer's
CD45, also known as protein tyrosine phosphatase, receptor type, C (PTPRC), was originally known as common leukocyte antigen and is a signal transducer involved in many physiological processes such as growth and differentiation, cancer transformation,...  Read full blog post.

Vimentin Antibodies in Rheumatoid Arthritis & Cataracts Research
Vimentin is a 57kDa type III intermediate filament (IF) protein that is the major cytoskeletal component of mesenchymal cells and the first to be expressed during cell differentiation. It plays a significant role in supporting and anchoring the positi...  Read full blog post.

Antibodies In The Differential Diagnosis Of Undifferentiated Tumors
Immunohistochemical testing using antibody panels has a valuable role in cancer diagnosis. Some tumors, especially more malignant ones, tend to lose the appearance of the tissue of origin. However, knowing the tissue of origin assists the physician in...  Read full blog post.

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Product PDFs


Gene Symbol PTPRC

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