CD45 Antibody (30-F11) [DyLight 550]


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Product Details

Reactivity MuSpecies Glossary
Applications Flow, IHC, IHC-Fr, IHC-P, IP
Please see the vial label for concentration. If unlisted please contact technical services.

CD45 Antibody (30-F11) [DyLight 550] Summary

Mouse thymus or spleen
Transmembrane Protein with Extracellular, Helical and Cytoplasmic Domains
CD45 (30-F11)
IgG2b Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C in the dark.
DyLight 550
DyLight 550 (A=562, E=576)
50mM Sodium Borate
0.05% Sodium Azide
Protein G purified


  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.25 microgram per one million cells in 100 microliter volume. Immunohistochemistry, It is recommended that the reagent be titrated for optimal performance for each application. NB100-77417 may also be used for immunoprecipitation, Western blot and immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections).


Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for CD45 Antibody (30-F11) [DyLight 550]

  • B220
  • CD45 antigen
  • CD45
  • CD45R
  • EC
  • LCA
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 Glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide


CD45 is a 180-240 kD glycoprotein, also known as the leukocyte common antigen (LCA), T200 or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell, as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD45 Antibody (NB100-77417R). (Showing 1 - 2 of 2 FAQ).

  1. We are interested in CD45 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • I would recommend one of these CD45 antibodies to you, as these have all been validated for use with dog/canine samples.
  2. We would like to order a CD45 antibody to stain immune cells that were isolated from a ligated sciatic nerve of mice with double immunofluorescence (using PFA-fixed tissue on slides). Which of the following rat monoclonal CD45 antibodies would you recommend: 30-F11, IBL-3/16 or 5C16?
    • I would recommend clone 30-F11. As clone IBL-3/16 has not yet been validated for IHC-P application, we would not be able to guarantee its workability on PFA-fixed tissue. Although both other clones i.e. 30-F11 and 5C16, would be good for your samples, CD45 30-F11 # NB100-77417 is a well known clone that offers more flexibility around protocol because of availability of its conjugated forms. It would be advantageous to use a conjugated primary as you are planning for double-immunostaining procedure.

Secondary Antibodies


Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-77417APC
DyLight 350 Labeled NB100-77417UV
DyLight 405 Labeled NB100-77417V
DyLight 405LS Labeled NB100-77417V3
DyLight 488 Labeled NB100-77417G
DyLight 650 Labeled NB100-77417C
DyLight 680 Labeled NB100-77417FR
DyLight 755 Labeled NB100-77417IR
PE Labeled NB100-77417PE
PerCP Labeled NB100-77417PCP

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Blogs on CD45.

CD45 - Much more than just a housekeeping protein
CD45, also known as T200 or the Leukocyte Common Antigen (LCA), is encoded by the Protein Tyrosine Phosphatase Receptor type C (PTPRC) gene. The protein is expressed exclusively on cells of the haematopoietic system, and is one of the most abundant le...  Read full blog post.

CD45 Isoforms: Hematopoietic Differentiation, Cancer and Alzheimer's
CD45, also known as protein tyrosine phosphatase, receptor type, C (PTPRC), was originally known as common leukocyte antigen and is a signal transducer involved in many physiological processes such as growth and differentiation, cancer transformation,...  Read full blog post.

Vimentin Antibodies in Rheumatoid Arthritis & Cataracts Research
Vimentin is a 57kDa type III intermediate filament (IF) protein that is the major cytoskeletal component of mesenchymal cells and the first to be expressed during cell differentiation. It plays a significant role in supporting and anchoring the positi...  Read full blog post.

Antibodies In The Differential Diagnosis Of Undifferentiated Tumors
Immunohistochemical testing using antibody panels has a valuable role in cancer diagnosis. Some tumors, especially more malignant ones, tend to lose the appearance of the tissue of origin. However, knowing the tissue of origin assists the physician in...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol PTPRC

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