TLR9 Antibody (26C593.2) - Azide Free


Western Blot: TLR9 Antibody (26C593.2) [Azide Free] [NBP2-24863] - Analysis of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine, and D) rat intestine tissue lysates using this antibody at a dilution of 3 more
Immunohistochemistry-Paraffin: TLR9 Antibody (26C593.2) [Azide Free] [NBP2-24863] - Formalin-fixed, paraffin-embedded human spleen probed with TLR9 antibody at 5 ug/ml. Human tissue was used for this test. Staining more
Flow Cytometry: TLR9 Antibody (26C593.2) [Azide Free] [NBP2-24863] - Intracellular flow analysis of TLR9 in Ramos cells using 0.5 ug of this antibody. Shaded histogram represents Ramos cells without antibody; green more

Product Details

Reactivity Hu, Mu, Rt, Ca, Eq, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-P
Please see the vial label for concentration. If unlisted please contact technical services.

TLR9 Antibody (26C593.2) - Azide Free Summary

This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-300 of human TLR9 isoform A (Genbank accession no. AAF78037).
IgG1 Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Antibody in PBS. Filter sterilized.
No Preservative
Please see the vial label for concentration. If unlisted please contact technical services.
Protein G purified


Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. In human PBMC, a ~120 kDa band is observed. A smaller isoform, TLR9 isoform B (Genbank accession no. AAF72190) containing 975 amino acids may also be observed in some cases.
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NBP2-24863 in the following applications:

Reactivity Notes

Rhesus Monkey.

Alternate Names for TLR9 Antibody (26C593.2) - Azide Free

  • CD289 antigen
  • CD289
  • toll-like receptor 9


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TLR9 Antibody (NBP2-24863)(54)

We have publications tested in 2 confirmed species: Human, Primate (Rhesus monkey).

We have publications tested in 6 applications: Flow-IC, IHC, IHC-Fr, IHC-P, In vitro, WB.

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In vitro
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Primate (Rhesus monkey)
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Showing Publications 1 - 10 of 54. Show All 54 Publications.
Publications using NBP2-24863 Applications Species
Guerrier T, Pochard P, Lahiri A et al. TLR9 expressed on plasma membrane acts as a negative regulator of human B cell response. J. Autoimmun. 2014 Feb 25 [PMID:24582318] (In vitro, Human) In vitro Human
Belmont L, Rabbe N, Antoine M et al. Expression of TLR9 in tumor-infiltrating mononuclear cells enhances angiogenesis and is associated with a worse survival in lung cancer. Int J Cancer. 2013 Aug 3 [PMID:23913633]
Figueiredo MM, Amorim IF, Pinto AJ et al. Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum. BMC Immunol. 2013 May 14 [PMID:23668673]
Abdi J, Mutis T, Garssen J, Redegeld F. Characterization of the Toll-like receptor expression profile in human multiple myeloma cells. PLoS One. 2013 Apr 8 [PMID:23593278]
Siponen M, Kauppila JH, Soini Y, Salo T. TLR4 and TLR9 are induced in oral lichen planus. J Oral Pathol Med. 2012 Nov [PMID:22672741]
Karim R, Meyers C, Backendorf C et al. Human papillomavirus deregulates the response of a cellular network comprising of chemotactic and proinflammatory genes. PLoS One. 2011 Mar 14 [PMID:21423754]
Dasgupta G, Chentoufi AA, You S et al. Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses. Invest Ophthalmol Vis Sci. 2011 May 17 [PMID:21273544]
van Gent M, Griffin BD, Berkhoff EG et al. EBV lytic-phase protein BGLF5 contributes to TLR9 downregulation during productive infection. J Immunol. 2011 Feb 1 [PMID:21191071]
Myles A, Aggarwal A. Expression of Toll-like receptors 2 and 4 is increased in peripheral blood and synovial fluid monocytes of patients with enthesitis-related arthritis subtype of juvenile idiopathic arthritis. Rheumatology (Oxford). 2011 Mar [PMID:21097451]
Schneberger D, Lewis D, Caldwell S, Singh B. Expression of toll-like receptor 9 in lungs of pigs, dogs and cattle. Int J Exp Pathol. 2011 Feb [PMID:21044185]
Show All 54 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP2-24729APC
Biotin Labeled NBP2-24890
DyLight 350 Labeled NBP2-24729UV
DyLight 405 Labeled NBP2-24729V
DyLight 405LS Labeled NBP2-24729V3
DyLight 488 Labeled NBP2-24729G
DyLight 550 Labeled NBP2-24729R
DyLight 650 Labeled NBP2-24729C
DyLight 680 Labeled NBP2-24729FR
DyLight 755 Labeled NBP2-24729IR
FITC Labeled NBP2-24908
HRP Labeled NBP2-24729H
PE Labeled NBP2-24907
PerCP Labeled NBP2-24729PCP

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Blogs on TLR9.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR9: For Whom the Cell Tolls
The Toll-like receptor 9 (TLR9) protein, also known as CD289, belongs to the family of Toll-like receptor (TLR) proteins which play a large role in pathogen recognition and the activation of innate immunity. Scientists using TLR9 antibodies have found...  Read full blog post.

TLR9: Tollgate to Immunity
Toll-like receptors (TLRs) play an essential role in the activation of innate immunity, and TLRs are expressed in a large number of immune cells as well as in epithelial cells. TLR9 recognizes synthetic oligodeoxynucleotides (ODN) containing unmethyla...  Read full blog post.

TLR9, Infectious Disease and Cancer
Toll-like receptor 9 (TLR9) is a protein encoded by TLR9 gene in humans. It is also known as cluster of differentiation 289 (CD289) and is a member of TLR family. Proteins from TLR family are transmembrane proteins that expressed in both antigen-resen...  Read full blog post.

TLR9 Antibodies in Immunity Research
Toll-like receptor 9 (TLR9) is a member of the toll-like receptor family that plays a key role in pathogen recognition and activation of innate immunity. Scientists using TLR9 antibodies have found the protein is highly conserved from Drosophila to hu...  Read full blog post.

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Product PDFs


Gene Symbol TLR9

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