TLR4 Antibody

Images

 
Western Blot: TLR4 Antibody [NBP2-24538] - Analysis of TLR4 in human intestine lysate in the 1) absence and 2) presence of immunizing peptide using TLR4 antibody at 0.1 ug/ml. I goat anti-rabbit Ig HRP secondary ...read more
Immunohistochemistry-Paraffin: TLR4 Antibody [NBP2-24538] - Human prostate stained with TLR4 antibody at 10 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Bv, Eq, Ft, Pm, RMSpecies Glossary
Applications WB, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TLR4 Antibody Summary

Immunogen
A portion of amino acids 650-700 of human TLR4 was used as the immunogen for this antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TLR4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1-0.5 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
Agonist
Antagonist
Publications
Read Publications using
NBP2-24538 in the following applications:

  • WB
    4 publications

Reactivity Notes

Reacts with Bison, Chimpanzee, Gorilla. Predicted reactivity with Rat, Dog

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TLR4 Antibody

  • ARMD10
  • CD284 antigen
  • CD284
  • hTollhomolog of Drosophila toll
  • TLR4
  • TOLL
  • toll-like receptor 4

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for TLR4 Antibody (NBP2-24538)(6)

We have publications tested in 4 confirmed species: Human, Mouse, Bovine, Ferret.

We have publications tested in 1 application: WB.


Filter By Application
WB
(4)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
Bovine
(1)
Ferret
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using NBP2-24538 Applications Species
Snyder JM, Wood TR, Corry K et al. Ontogeny of white matter, toll-like receptor expression, and motor skills in the neonatal ferret Int. J. Dev. Neurosci. May 20 2018 [PMID: 29791868] (Ferret) Ferret
Ellison MA, Gearheart CM, Porter CC, Ambruso DR. IFN-g alters the expression of diverse immunity related genes in a cell culture model designed to represent maturing neutrophils. PLoS ONE. 2017 Oct 05 [PMID: 28982143] (WB, Human) WB Human
Wu T, Wang C, Ding L et al. Arginine Relieves the Inflammatory Response and Enhances the Casein Expression in Bovine Mammary Epithelial Cells Induced by Lipopolysaccharide. Mediators of Inflammation. 2016 Feb 24 [PMID: 27110069] (WB, Bovine) WB Bovine
Wu Y, Lu J, Antony S et al. Activation of TLR4 is required for the synergistic induction of dual oxidase 2 and dual oxidase A2 by IFN-G and lipopolysaccharide in human pancreatic cancer cell lines. J Immunol. 2013 Feb 15 [PMID: 23296709] (WB, Human)

Details:
Products cited: QuikChIP Kit (cat. no. 3010K) immunoprecipiation of NF-kB in BxPC-3 cells, anti-human TLR4 Ab (cat. no. IMG-6307A) WB AsPC-1 and BxPC-3 cells, anti-human TLR4 FITC Ab (cat. no. IMG-5031C) Flow cell surface BxPC-3 cells treated with IFN-y,
WB Human
Torgersen Maria L, Engedal Nikolai, Pedersen Anne-Mari G et al. Toll-like receptor 4 facilitates binding of Shiga toxin to colon carcinoma and primary umbilical vein endothelial cells. FEMS Immunol Med Microbiol. 2011 Feb [PMID: 21054580] (Human) Human
Park HJ, Hong JH, Kwon HJ et al. TLR4-mediated activation of mouse macrophages by Korean mistletoe lectin-C (KML-C). Biochem Biophys Res Commun. 2010 Jun 4 [PMID: 20450885] (WB, Mouse)

Details:
The following antibodies were used: TLR4/CD284 (IMG-428E), TLR4/CD284 (IMG-577), and TLR4 (IMG-6307A). IMG-577 & IMG-6307A were used in Fig 1 (WB): Peritoneal primary mouse (BALB/C) macrophages stimulated with LPS and then treated with KML-C (Korean mistl
WB Mouse

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Blogs on TLR4. Showing 1-10 of 11 blog posts - Show all blog posts.

PAMPs and DAMPs: What is the same and what is different about these molecules?
By Victoria OsinskiWhat are PAMPs and DAMPsInflammation results from stimuli signaling damage or infection. The inflammatory response can be beneficial or harmful depending on the type and duration of stimuli. The s...  Read full blog post.

How to identify B cell subsets using flow cytometry
By Victoria OsinskiUsing flow cytometry to identify B cell subsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified a...  Read full blog post.

Lipopolysaccharide from gut microbiome localizes in human atherosclerotic plaques and promotes TLR4-mediated oxidative stress
By Jamshed Arslan, Pharm. D., PhD. Atherosclerosis is a chronic inflammatory condition in which plaques of fats and other substances slowly buildup on the inner walls of arteries to restrict blood flow. In atheroscle...  Read full blog post.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol TLR4
Uniprot