TLR4 Antibody

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Western Blot: TLR4 Antibody [NB100-56579] - Analysis of TLR4 using this antibody on (A) 1 ug/lane recombinant mouse TLR4 protein (tested at 2 ug/ml), (B) 20 ug/lane human intestine, and (C) 20 ug/lane mouse intestine ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TLR4 Antibody Summary

Immunogen
A portion of amino acids 100-150 of human TLR4 was used as the immunogen.
Specificity
Observed size: approx. 90 kD (native) or 80 kD (partial recombinant mouse TLR4--extracellular portion plus His-tag)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TLR4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-5 ug/ml
  • Immunoprecipitation 1:10-1:500
Application Notes
Immunoprecipitation: Please see Ueta et (2004) for details.
Theoretical MW
96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Agonist
Antagonist
Control
Mouse Small Intestine Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 1 Review rated 5
using
NB100-56579 in the following applications:

Publications
Read Publications using
NB100-56579 in the following applications:

  • IP
    2 publications
  • WB
    5 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TLR4 Antibody

  • ARMD10
  • CD284 antigen
  • CD284
  • hTollhomolog of Drosophila toll
  • TLR4
  • TOLL
  • toll-like receptor 4

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling. The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TLR4 Antibody (NB100-56579)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IP, WB.


Filter By Application
IP
(2)
WB
(5)
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(1)
Mouse
(4)
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Showing Publications 1 - 9 of 9.
Publications using NB100-56579 Applications Species
Moreth K, Frey H, Hubo M et al. Biglycan-triggered TLR-2- and TLR-4-signaling exacerbates the pathophysiology of ischemic acute kidney injury. Matrix Biol. 2014 Jan 28 [PMID: 24480070] (WB, Mouse)

Details:
Murine peritoneal macrophages, Fig 1C. Cells were IPd with biglycan, followed by WB with the TLR4 pAb. The IP/WB results showed that that TLR4 co-IPd with biglycan, indicating protein-protein interactions between biglycan and TLR4. TLR4 was detected at 95
WB Mouse
Jin Yingji, Tachibana Isao, Takeda Yoshito et al. Statins decrease lung inflammation in mice by upregulating tetraspanin CD9 in macrophages. PLoS One. 2013 [PMID: 24040034] (WB, Mouse) WB Mouse
Krishnan S, Chen S, Turcatel G et al. Regulation of Toll-like receptor 2 interaction with Ecgp96 controls Escherichia coli K1 invasion of brain endothelial cells Cell Microbiol. 2013 Jan [PMID: 22963587] (WB, Human) WB Human
Merline Rosetta, Moreth Kristin, Beckmann Janet et al. Signaling by the matrix proteoglycan decorin controls inflammation and cancer through PDCD4 and MicroRNA-21. Sci Signal. 2011 [PMID: 22087031] (IP) IP
Park HJ, Hong JH, Kwon HJ et al. TLR4-mediated activation of mouse macrophages by Korean mistletoe lectin-C (KML-C). Biochem Biophys Res Commun. 2010 Jun 4 [PMID: 20450885] (WB, Mouse)

Details:
The following antibodies were used: TLR4/CD284 (IMG-428E), TLR4/CD284 (IMG-577), and TLR4 (IMG-6307A). IMG-577 & IMG-6307A were used in Fig 1 (WB): Peritoneal primary mouse (BALB/C) macrophages stimulated with LPS and then treated with KML-C (Korean mistl
WB Mouse
Bansal K, Elluru SR, Narayana Y et al. PE_PGRS antigens of Mycobacterium tuberculosis induce maturation and activation of human dendritic cells. J Immunol. 2010 Apr 1 [PMID: 20176745] (WB)

Details:
Products cited: The following antibodies were used for WB in Fig 4B, 4C using HEK-293 cells transiently transfected with TLR2 or TLR2 dominant negative constructs: 1. TLR1 (IMG-5012), 2. TLR2 IMG-(6320A), 3. TLR4 (IMG-577), 4.TLR6 (IMG-527). Note: TLR2 wa
WB
Suzuki Mayumi, Tachibana Isao, Takeda Yoshito et al. Tetraspanin CD9 negatively regulates lipopolysaccharide-induced macrophage activation and lung inflammation. J Immunol. 2009 May 15 [PMID: 19414803] (Mouse) Mouse
Gandhi Shiv K, Siliciano Janet D, Bailey Justin R et al. Role of APOBEC3G/F-mediated hypermutation in the control of human immunodeficiency virus type 1 in elite suppressors. J Virol. 2008 Mar [PMID: 18077705]
Ueta M, Nochi T, Jang MH et al. Intracellularly expressed TLR2s and TLR4s contribution to an immunosilent environment at the ocular mucosal epithelium. J Immunol. 2004 Sep 1 [PMID: 15322197] (IP)

Details:
1. TLR4 (IMG-577) [IP, see results section].
IP

Review for TLR4 Antibody (NB100-56579) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56579:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 12/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22963587
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22963587

Details

Detection NotesSee PMID 22963587

Comments

CommentsPublished in PMID: 22963587

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TLR4 Antibody (NB100-56579) (0)

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Control Lysate(s)

Secondary Antibodies

 

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Blogs on TLR4. Showing 1-10 of 11 blog posts - Show all blog posts.

PAMPs and DAMPs: What is the same and what is different about these molecules?
By Victoria OsinskiWhat are PAMPs and DAMPsInflammation results from stimuli signaling damage or infection. The inflammatory response can be beneficial or harmful depending on the type and duration of stimuli. The s...  Read full blog post.

How to identify B cell subsets using flow cytometry
By Victoria OsinskiUsing flow cytometry to identify B cell subsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified a...  Read full blog post.

Lipopolysaccharide from gut microbiome localizes in human atherosclerotic plaques and promotes TLR4-mediated oxidative stress
By Jamshed Arslan, Pharm. D., PhD. Atherosclerosis is a chronic inflammatory condition in which plaques of fats and other substances slowly buildup on the inner walls of arteries to restrict blood flow. In atheroscle...  Read full blog post.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.

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Recent Reviews

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Anonymous
12/12/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol TLR4
Entrez
Uniprot