TLR4 Antibody (76B357.1) [Allophycocyanin]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Baboon, Bovine, Porcine, Chinese HamsterSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-P
Clone
76B357.1
Clonality
Monoclonal
Host
Mouse

TLR4 Antibody (76B357.1) [Allophycocyanin] Summary

Immunogen
This antibody was developed against a portion of amino acids 100-200 of human TLR4 (NP_612564).
Specificity
The amino acid sequence used has 100% identity in human, chimp, baboon, chinese hamster, and rat; 93% identity in bovine and pig; 85% identity in sheep and 78% identity in cat, horse and mouse.
Predicted Species
Porcine (93%), Chinese Hamster (100%), Bovine (93%), Baboon (100%). Backed by our 100% Guarantee.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TLR4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
Allophycocyanin
Format
Allophycocyanin (A=620-650, E=660-670)
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry 1ul/1 million cells
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin
Application Notes
Flow (Intracellular) use reported in literature (See Cohen et al, 2008); Immunofluorescence (See Nowicki et al, 2009); IHC-F (Nowicki et al, 2012); Flow (cell surface) use reported in scientific literature (PMID 23796194)

Reactivity Notes

Ground squirrel reactivity reported by a customer review. Predicted cross-reactivity based on sequence identity: Chimp (100%), Baboon (100%), Chinese Hamster (100%), Bovine (93%), Pig (93%). Mammal reactivity reported in scientific literature (PMID: 25130694)

Alternate Names for TLR4 Antibody (76B357.1) [Allophycocyanin]

  • ARMD10
  • CD284 antigen
  • CD284
  • hTollhomolog of Drosophila toll
  • TLR4
  • TOLL
  • toll-like receptor 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR4 Antibody (NBP2-27149APC) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 647 Labeled NBP2-24773
Biotin Labeled NBP2-27149B
DyLight 350 Labeled NBP2-27149UV
DyLight 405 Labeled NBP2-27149V
DyLight 405LS Labeled NBP2-27149V3
DyLight 488 Labeled NBP2-27149G
DyLight 550 Labeled NBP2-27149R
DyLight 650 Labeled NBP2-27149C
DyLight 680 Labeled NBP2-27149FR
DyLight 755 Labeled NBP2-27149IR
FITC Labeled NB100-55951
HRP Labeled NBP2-27149H
PE Labeled NBP2-27149PE
PerCP Labeled NBP2-27149PCP

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Blogs on TLR4.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR4: Playing Roles in Apoptosis and Autoimmunity
TLR4 (Toll-like receptor 4) is a member of the Toll-like receptor (TLR) family that plays a key role in pathogen recognition and activation of innate immunity. Scientists have found that TLRs are highly conserved from Drosophila to humans, with a high...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol TLR4
Entrez

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