TLR2 Antibody (TL2.3)

Immunohistochemistry-Frozen: TLR2 Antibody (TL2.3) [NBP1-51793] - IHC-F using this antibody

Product Discontinued

TLR2 Antibody (TL2.3) Summary

• Immunogen: Human TLR2-expressing CHO cells
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TLR2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunoassay
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: This product works in Flow Cytometry, Functional, Immunoassay, Immunofluorescence, Immunohistochemistry-Frozen and Western Blot.
• Agonist:
  • Pam3CSK4, TLR1 and TLR2 Ligand NBP2-25297
  • SUCNR1/GPR91 Antibody NBP2-26239
  • MALP-2, TLR6 and TLR2 ligand NBP2-26219
• Antagonist:
  • TIRAP (TLR2 and TLR4) Inhibitor Peptide Set NBP2-29331

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.2 um filtered solution in PBS and 0.1% BSA
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for TLR2 Antibody (TL2.3)

• CD282 antigen
• CD282
• TIL4CD282
• TLR2
• toll/interleukin 1 receptor-like 4
• Toll/interleukin-1 receptor-like protein 4
• toll-like receptor 2

Background

The monoclonal antibody TLR3.7 recognizes the 116 kDa human Toll-like receptor 3 (TLR3, CD283). Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called 'pathogen-associated molecular patterns'. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NFB-dependent and IFN-regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. TLRs are class I receptors, with a single a-helix that spans the cell membrane. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens, such as lipopolysaccharide (LPS) from the outer membrane of Gram negative bacteria, peptidoglycan fragments from bacterial cell walls and single-stranded and double-stranded RNA from viruses. Some forms of RNA and DNA from pathogens exhibit immutable features that distinguish them from nucleic acids of higher organisms. For example, dsRNA, is a common intermediate of viral replication and a potent indicator of infection. Toll-like receptor 3 (TLR3) recognizes viral double-stranded RNA and its synthetic analog polyriboinosinic:polyribocytidylic acid (poly(I:C)). TLR3 is normally located in acidic endosomes where its luminal ectodomain (ECD) encounters dsRNA and induces type I interferon (IFN), inflammatory cytokine/chemokine production and dendritic cell (DC) maturation via the adaptor protein TICAM-1 (also called TRIF). Based on the different subcellular localization of cytosolic RNA receptors and TLR3, these receptors seem to play distinct roles in anti-viral immune responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TLR2