Synaptophysin Antibody - BSA Free

Western Blot: Synaptophysin Antibody [NBP2-25170] - Analysis of Synaptophysin in (A) human brain lysate and (B) mouse brain lysate.

Immunocytochemistry/Immunofluorescence: Synaptophysin Antibody [NBP2-25170] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Synaptophysin at a 1:50 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: Synaptophysin Antibody [NBP2-25170] - Analysis of Synaptophysin in mouse brain.

• Reactivity: Hu, Mu, Pm, Rt, Bv, Sq
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Synaptophysin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Synaptophysin Antibody - BSA Free (NBP2-25170) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-Synaptophysin Antibody: Cited in 10 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to an C-terminal portion of the human Synaptophysin protein (between residues 230-313) [UniProt# P08247]
• Localization: Golgi, cytoplasmic and membrane
• Marker: Pre-synaptic marker
• Predicted Species: Rat (95%), Bovine (96%), Squirrel (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SYP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:250
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 31117227)
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1.0 ug/ml
• Control:

  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Synaptophysin Antibody - BSA Free

• Major synaptic vesicle protein p38
• MRX
• MRXSYP
• Synaptophysin
• SYP

Background

Communication in the nervous system is dependent on the exocytosis of neurotransmitter-containing synaptic vesicles that release their contents at the synapses following membrane depolarization. Synaptophysin (SYP) is a major 38 kDa tetraspan membrane protein of small synaptic vesicles that plays an essential role in targeting synaptic vesicles to the presynaptic membrane. Synaptophysin directly interacts with the essential membrane fusion v-SNARE protein, synaptobrevin/VAMP2, and targets it to the presynaptic membrane (PMID: 14528015). Thus, synaptophysin is required for proper targeting of VAMP2 and synaptic vesicles. Synaptophysin is localized to the trans-Golgi cisternae and then accumulates to the presynaptic membrane in a microtubule dependent manner (PMID: 8227215).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Synaptophysin Antibody (NBP2-25170)(11)

Publications using NBP2-25170

• Huynh B Brainstem Neurotransmitter System Deterioration in Parkinson's Disease and Parkinson's Disease with Dementia Thesis 2023-01-01 (WB, Human)
• He B, Gao R, Lv S et al. Cancer cell employs a microenvironmental neural signal trans-activating nucleus-mitochondria coordination to acquire stemness Signal transduction and targeted therapy 2023-07-19 [PMID: 37463926] (ICC/IF, Human)
• Lima BHM, Bombeiro AL, Cartarozzi LP, Oliveira ALR. The Time Course of MHC-I Expression in C57BL/6J and A/J Mice Correlates with the Degree of Retrograde Gliosis in the Spinal Cord following Sciatic Nerve Crush Cells 2022-11-22 [PMID: 36496969] (Immunohistochemistry-Paraffin, Mouse)
• Ignarro RS, Bombeiro AL, Chiarotto GB et al. Interferon-beta induces major histocompatibility complex of class I (MHC-I) expression and a proinflammatory phenotype in cultivated human astrocytes Differentiation; research in biological diversity 2022-10-15 [PMID: 36306693] (FLOW, Rat)
  
  Details:
  Dilution used in Flow 1:100
• Ramasubramanian B, Griffith C, Hanson M et al. Protective Effects of Chaya against Mitochondrial and Synaptic Toxicities in the Type 2 Diabetes Mouse Model TallyHO Cells 2022-02-21 [PMID: 35203393] (WB, IF/IHC, Mouse)
• Cartarozzi LP, Perez M, Fernandes GG et al. Neuroprotection and gliosis attenuation by intravenous application of human mesenchymal stem cells (hMSC) following ventral root crush in mice Molecular and cellular neurosciences 2021-12-24 [PMID: 34954382] (IF/IHC)
• Awasthi S, Hindle A, Sawant NA Et al. RALBP1 in Oxidative Stress and Mitochondrial Dysfunction in Alzheimer's Disease Cells 2021-11-10 [PMID: 34831336]
• Toro CA, Hansen J, Siddiq MM et al. The Human ApoE4 Variant Reduces Functional Recovery and Neuronal Sprouting After Incomplete Spinal Cord Injury in Male Mice Frontiers in Cellular Neuroscience 2021-02-18 [PMID: 33679326] (Immunohistochemistry-Paraffin, Mouse)
• Chavez-Valdez R, Lechner C, Emerson P et al. Accumulation of PSA-NCAM marks nascent neurodegeneration in the dorsal hippocampus after neonatal hypoxic-ischemic brain injury in mice J. Cereb. Blood Flow Metab. 2020-07-23 [PMID: 32703109]
• Cartarozzi LP, Perez M, Kirchhoff F, Oliveira ALR Role of MHC-I Expression on Spinal Motoneuron Survival and Glial Reactions Following Ventral Root Crush in Mice Cells 2019-05-21 [PMID: 31117227] (IHC-Fr, Mouse)
• Bernardes D, Rodrigues de Oliveira AL Regular Exercise Modifies Histopathological Outcomes of Pharmacological Treatment in Experimental Autoimmune Encephalomyelitis Frontiers 2019-11-20 [PMID: 30524355] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: SYP
• Entrez:
  • 6855(Human)
  • 20977(Mouse)
• Uniprot:
  • P08247()
  • Q62277(Mouse)