GAP-43 Antibody

Immunohistochemistry-Paraffin: GAP-43 Antibody [NB300-143] - Review image from confirmed customer on mouse E15.5 paraffin sections.

Western Blot: GAP-43 Antibody [NB300-143] - Different tissue and cell lysates using rabbit pAb to GAP43, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] SH-SY5Y cells, [7] C6 cells. Single band at 43 kDa mark corresponds to GAP43 protein. The GAP43 protein is detected only in the lysates of neuronal origin. C6 cells are a rat glioma cell line and do not express GAP43 protein

Immunohistochemistry: GAP-43 Antibody [NB300-143] - Representative images of immunohistological staining (brown) of OMP-positive (OMP+) cells, GAP43+ immature ORNs. Tissue sections were counterstained with the nuclear dye hematoxylin (blue). Numbers of SOX2+ ORN progenitors and Ki67+ actively proliferating cells per mm of the basal layer and OMP+ mature ORNs, GAP43+ immature ORNs, and Cas3+ apoptotic cells per mm of the OE in saline or rhIGF-1-treated mice. Open circles, rectangles, and triangles represent the values for each mouse in the saline, low-IGF-1, and high-IGF-1 treated groups (each n = 6), respectively. Image collected and cropped by Citeab from the following publication (Dose-Dependent Effects of Insulin-Like Growth Factor 1 in the Aged Olfactory Epithelium. Front Aging Neurosci (2018)) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: GAP-43 Antibody [NB300-143] - Posterior MOE sections at approximately the same region where EOG was recorded were obtained from control and Skn-1a-/- mice exposed to either water or chemicals for 2 wks. The sections were immunoreacted with antibodies against GAP43 and OMP and stained with DAPI. Confocal images were taken from olfactory turbinate II. Similar morphology and marker expression were found in four groups of mice, indicating there was no obvious tissue damage in both control and Skn-1a-/- mice after chemical exposure in the posterior MOE regions where EOG recordings were performed. Scale bar: 50 um. Image collected and cropped by CiteAb from the following publication (//eneuro.sfn.org/lookup/doi/10.1523/ENEURO.0135-17.2017), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB300-143] - Cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to GAP43, dilution 1:2,000 in green, and costained with mouse mAb to vimentin, dilution 1:2,000, in red. Blue: DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of neuronal cells, while vimentin antibody stains intermediate filaments in fibroblasts and other non-neuronal cells.

Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB300-143] - Rat E18 mixed neuron/glia cultures with rabbit GAP43 (red) and 5B10, mouse monoclonal to MAP-tau (green).

Western Blot: GAP-43 Antibody [NB300-143] - Western blots of homogenate of cow cerebellum stained with RPCA-GAP-43 . A prominent band running at ~43kDa represents the full length GAP-43.

Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB300-143] - Immunofluorescence of GAP-43 (green), a molecular marker of neurite outgrowth, demonstrates intense staining in overexpressing wild-type PS-1 (E) PC-12 cells. (Teo, et al, 2005)

Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB300-143] - Mixed neuron-glial cultures stained with RPCA-GAP43 (red), blue is DNA staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Eq, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

GAP-43 Antibody Summary

• Immunogen: C-terminal peptide of rodent GAP43, KEDPEADQEHA coupled to KLH.
• Localization: Neuronal Marker, developing and regenerating axons and growth cone.
• Marker: Neuronal Marker
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GAP43
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Frozen 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1:10000
• Application Notes: This GAP43 antibody is useful for Immunocytochemistry/Immunofluorescence, and Western blot, where it recognizes a band at 43 kDa. Use in IHC-P and IHC-Fr reported in scientific literature (PMID: 29950987 and 27007292 respectively). Use in FLOW reported in scientific literature (PMID: 21614130).
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reactivity to Canine, Chicken, and Primate reported in scientific literature (PMID: 30647968, 30819546, and 24249398 respectively). Drosophila reactivity reported in scientific literature (PMID: 30819546).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 5mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GAP-43 Antibody

• Axonal membrane protein GAP-43
• B-50
• Basp2
• calmodulin-binding protein P-57
• GAP43
• GAP-43
• growth associated protein 43
• Growth-associated protein 43
• nerve growth-related peptide GAP43
• Neural phosphoprotein B-50
• Neuromodulin
• neuron growth-associated protein 43
• PP46
• protein F1

Background

GAP43 (growth associated protein 43, also called B-50, PP46, calmodulin-binding protein P-57, neuromodulin, neuron growth-associated protein 43, protein F1) is a major component of motile "growth cones" that form the tips of elongating axons as well as the synaptic plasma membranes. GAP43 is associated with nerve growth process and plays a key role in axonal as well as dendritic filopodia induction. It has been detected in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN, and binds calmodulin with superior affinity in the absence of Ca2+ than in its presence. Intracellular Ca2+ regulates PKC mediated phosphorylation of GAP43 and affects GAP43's ability to bind calmodulin. GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex. Aberrant expression of GAP43 has been seen in patients diagnosed with schizophrenia and Alzheimer's disease. Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 was independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual SDS-PAGE mobility properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14 kDa. GAP43 is one of many highly negatively charged extended molecules which lack well defined tertiary structure and contain few hydrophobic residues and which run anomalously on SDS-PAGE. Other examples are CAP23, MARCKS, microtubule associated proteins MAP2, tau and the Neurofilament subunits. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GAP-43 Antibody (NB300-143)(94)

Publications using NB300-143

• Shayya H The unfolded protein response couples neuronal identity to circuit formation in the developing mouse olfactory system Thesis 2023-01-01 (IF/IHC, Mouse)
• Cain MD, Klein NR, Jiang X, Klein RS. Post-exposure intranasal IFN? suppresses replication and neuroinvasion of Venezuelan Equine Encephalitis virus within olfactory sensory neurons bioRxiv 2023-07-07 [PMID: 37425867] (ICC/IF, IHC)
• Huang SWJ Neurogenesis in the Olfactory System: The Functional Activity of Immature Olfactory Sensory Neurons and Postnatal-Born Granule Cells Thesis 2022-01-01 (IHC-Fr, Mouse)
• Zhang Y Morphogenetic roles of Epb41l5 and cell heterogeneity in early kidney development Thesis 2022-01-01 (IHC-P, Mouse)
  
  Details:
  Dilution used for IHC 1:200
• Hanell M Optimization of protocol for immunofluorescence stain to observe nerve infiltration and regeneration in cancer tissue Thesis 2022-01-01
• Balakrishnan A Glial cells in health and disease Thesis 2020-01-01 (ICC/IF, Mouse)
• Acton L Investigating Mechanisms Responsible for the Pro-Regenerative Effects of Conditioning Electrical Stimulation Thesis 2022-01-01 (IHC-Fr, Mouse)
  
  Details:
  Adult 129S6/SvEvTac (wildtype) mice, IHC-Fr dilution used 1:500
• Tamaru T, Kobayakawa K, Saiwai H et al. Glial scar survives until the chronic phase by recruiting scar-forming astrocytes after spinal cord injury Experimental neurology 2023-01-01 [PMID: 36336030] (IHC-Fr, Mouse)
  
  Details:
  Dilution used in IHC-Fr 1:500
• Huang JS, Kunkhyen T, Rangel AN et al. Immature olfactory sensory neurons provide behaviourally relevant sensory input to the olfactory bulb Nature communications 2022-10-19 [PMID: 36261441] (IHC-Fr, Mouse)
  
  Details:
  Dilution used in IHC 1:1000
• Efraim Y, Chen FYT, Cheong KN et al. A synthetic tear protein resolves dry eye through promoting corneal nerve regeneration Cell reports 2022-08-30 [PMID: 36044852] (IF/IHC, Mouse)
  
  Details:
  Dilutions: 1:400
• Mohanta SK, Peng L, Li Y et al. Neuroimmune cardiovascular interfaces control atherosclerosis Nature [PMID: 35477759] (IHC-P, Mouse)
• Ching K, Wang JT, Stearns T Long-range migration of centrioles to the apical surface of the olfactory epithelium eLife 2022-04-14 [PMID: 35420544] (ICC/IF, Mouse)
• Mazuelas H, MagallOn-Lorenz M, FernAndez-RodrIguez J et al. Modeling iPSC-derived human neurofibroma-like tumors in mice uncovers the heterogeneity of Schwann cells within plexiform neurofibromas Cell reports 2022-02-15 [PMID: 35172160] (Mouse)
• Pototskiy E, Vinokuroff K, Ojeda A Et al. Downregulation of CD40L-CD40 attenuates seizure susceptibility and severity of seizures Scientific reports 2021-08-26 [PMID: 34446808] (IF/IHC, Mouse)
• Paramos-De-Carvalho D, Martins I, Cristovao Am Et Al. Targeting senescent cells improves functional recovery after spinal cord injury Cell reports 2021-07-06 [PMID: 34233184]
• Li F, Lo TY, Miles L et al. The Atr-Chek1 pathway inhibits axon regeneration in response to Piezo-dependent mechanosensation Nature Communications 2021-06-22 [PMID: 34158506] (IHC)
• Zhu YT, Zhang Q, Xie HY et al. Environmental enrichment combined with fasudil promotes motor function recovery and axonal regeneration after stroke Neural regeneration research 2021-12-01 [PMID: 33907042] (IF/IHC, Mouse)
• Deng WW, Wu GY, Min LX et al. Optogenetic Neuronal Stimulation Promotes Functional Recovery After Spinal Cord Injury Frontiers in neuroscience 2021-04-09 [PMID: 33897353] (WB, Rat)
• Cao W, Zhang C, Chen R et al. A Novel Cerebroprotein Hydrolysate, CH1, Ameliorates Chronic Focal Cerebral Ischemia Injury by Promoting White Matter Integrity via the Shh/Ptch-1/Gli-1 Signaling Pathway Neuropsychiatric disease and treatment 2020-12-23 [PMID: 33380798] (WB, Mouse)
• Zhang C, Cui L, He W et al. Dl-3-n-butylphthalide promotes neurite outgrowth of primary cortical neurons by Sonic Hedgehog signaling via upregulating Gap43 Experimental cell research 2020-12-06 [PMID: 33296663] (ICC/IF, Mouse)
• Niu J, Sanders SS, Jeong HK et al. Coupled Control of Distal Axon Integrity and Somal Responses to Axonal Damage by the Palmitoyl Acyltransferase ZDHHC17 Cell Rep 2020-11-17 [PMID: 33207199]
• Sanders SS, Hernandez LM, Soh H et al. The palmitoyl acyltransferase ZDHHC14 controls Kv1-family potassium channel clustering at the axon initial segment eLife 2020-11-13 [PMID: 33185190]
• Collura KM, Niu J, Sanders SS et al. The palmitoyl acyltransferases ZDHHC5 and ZDHHC8 are uniquely present in DRG axons and control retrograde signaling via the Gp130/JAK/STAT3 pathway J. Biol. Chem. 2020-09-21 [PMID: 32958558] (WB, Rat)
• Pal-Ghosh S, Tadvalkar G, Karpinski BA, Stepp MA Diurnal Control of Sensory Axon Growth and Shedding in the Mouse Cornea Invest. Ophthalmol. Vis. Sci. 2020-09-01 [PMID: 32870244] (ICC/IF, Mouse)
• Liu Y, Hall B, Lwin N et Al. Tissue Responses and Wound Healing following Laser Scleral Microporation for Presbyopia Therapy Transl Vis Sci Technol 2020-08-21 [PMID: 32818094]
• Hasegawa-Ishii S, Imamura F, Nagayama S et Al. Differential effects of nasal inflammation and odor deprivation on layer-specific degeneration of the mouse olfactory bulb eNeuro 2020-03-20 [PMID: 32220858] (Mouse)
• Merino P, Diaz A, Torre ER, Yepes M Urokinase-type plasminogen activator (uPA) regulates the expression and function of growth-associated protein 43 (GAP-43) in the synapse J. Biol. Chem. 2020-01-10 [PMID: 31819012] (ICC/IF, WB, Mouse)
• Bandeira, F, Yam, G H Et al. Three-Dimensional Neurite Characterization of Small Incision Lenticule Extraction Derived Lenticules. Invest Ophthalmol Vis Sci 2019-10-01 [PMID: 31639827] (ICC/IF, Drosophila melanogaster)
  
  Details:
  Citation using the Janiela Fluor 549 format of this antibody.
• Blaszkiewicz M, Willows JW, Dubois AL et al. Neuropathy and neural plasticity in the subcutaneous white adipose depot PLoS ONE 2019-09-11 [PMID: 31509546] (WB, Mouse)
• Azmi S, Jeziorska M, Ferdousi M et al. Early nerve fibre regeneration in individuals with type 1 diabetes after simultaneous pancreas and kidney transplantation Diabetologia 2019-06-07 [PMID: 31175373] (IF/IHC, Human)
• Zhou Y, Miles JR, Tavori H et al. Callosal septa express guidance cues and are paramedian guideposts for human corpus callosum development J. Anat. 2019-05-09 [PMID: 31070791] (WB)
• Sanders, SS;De Simone, FI;Thomas, GM; mTORC1 Signaling Is Palmitoylation-Dependent in Hippocampal Neurons and Non-neuronal Cells and Involves Dynamic Palmitoylation of LAMTOR1 and mTOR Front Cell Neurosci 2019-04-02 [PMID: 31001086] (WB, Human)
• Song Y, Li D, Farrelly O et al. The Mechanosensitive Ion Channel Piezo Inhibits Axon Regeneration Neuron 2019-02-15 [PMID: 30819546] (IF/IHC, Drosophila)
• Wang W, Jiang Z, Lu R et al. Effects of Renal Denervation via Renal Artery Adventitial Cryoablation on Atrial Fibrillation and Cardiac Neural Remodeling Cardiology Research and Practice 2018-12-11 [PMID: 30647968] (IHC-P, Canine)
• Ueha R, Kondo K, Ueha S, Yamasoba T. Dose-Dependent Effects of Insulin-Like Growth Factor 1 in the Aged Olfactory Epithelium Front Aging Neurosci 2018-11-20 [PMID: 30515092] (IF/IHC, Mouse)
• Wu Y, Ma L, Duyck K et al. A Population of Navigator Neurons Is Essential for Olfactory Map Formation during the Critical Period Neuron 2018-10-01 [PMID: 30482691]
• Renziehausen A, Wang H, Rao B et al. The renin angiotensin system (RAS) mediates bifunctional growth regulation in melanoma and is a novel target for therapeutic intervention Oncogene 2018-11-26 [PMID: 30478450] (WB, Human)
• Perry RBT, Hezroni H, Goldrich MJ, Ulitsky I. Regulation of Neuroregeneration by Long Noncoding RNAs. Molecular cell. [PMID: 30401432] (ICC/IF, Mouse)
• Zhang XM, Huang Y, Cong X et al. Parasympathectomy increases resting secretion of the submandibular gland in minipigs in the long term. J. Cell. Physiol. 2018-11-01 [PMID: 30387129] (IF/IHC, Porcine)
• Sahoo PK, Lee SJ, Jaiswal PB et al. Axonal G3BP1 stress granule protein limits axonal mRNA translation and nerve regeneration Nat Commun 2018-08-22 [PMID: 30135423] (ICC/IF, Rat)
• Wei YL, Hua J, Liu XY et al. LncNEN885 inhibits epithelial-mesenchymal transition by partially regulation of Wnt/B-catenin signalling in gastroenteropancreatic neuroendocrine neoplasms. Cancer Sci. 2018-10-01 [PMID: 30033597] (Human)
• Ueha R, Ueha S, Kondo K et al. Cigarette Smoke-Induced Cell Death Causes Persistent Olfactory Dysfunction in Aged Mice Front Aging Neurosci 2018-06-13 [PMID: 29950987] (IHC-P, Mouse)
• Xiong, L, Liu, Y Et al. Targeted ablation of cardiac sympathetic neurons attenuates adverse postinfarction remodelling and left ventricular dysfunction. Exp Physiol 2018-09-01 [PMID: 29928790] (WB, Mouse)
• Boboila S, Lopez G, Yu J et al. Transcription factor activating protein 4 is synthetically lethal and a master regulator of MYCN-amplified neuroblastoma Oncogene 2018-06-07 [PMID: 29880876] (WB, Human)
• Schmitd LB, Beesley LJ, Russo N et al. Redefining Perineural Invasion: Integration of Biology With Clinical Outcome Neoplasia 2018-05-22 [PMID: 29800815] (IF/IHC, Human)
• Zechel S, Fernandez-Suarez D, Ibanez CF. Cell-autonomous role of GFRa1 in the development of olfactory bulb GABAergic interneurons Biol Open 2018-05-18 [PMID: 29716946] (IF/IHC, Mouse)
• Ueha R, Shichino S, Ueha S et al. Reduction of Proliferating Olfactory Cells and Low Expression of Extracellular Matrix Genes Are Hallmarks of the Aged Olfactory Mucosa. Front Aging Neurosci 2018-03-27 [PMID: 29636678] (IF/IHC, Mouse)
• Cui Y, Yao Y, Zhao Y et al. Functional collagen conduits combined with human mesenchymal stem cells promote regeneration after sciatic nerve transection in dogs. J Tissue Eng Regen Med 2018-05-01 [PMID: 29499096]
• Hines TJ, Gao X, Sahu S, Lange MM. An Essential Postdevelopmental Role for Lis1 in Mice. eNeuro. 2018-02-02 [PMID: 29404402] (ICC/IF, Mouse)
• Kosi N, Alic I, Salamon I, Mitrecic D. Stroke promotes survival of nearby transplanted neural stem cells by decreasing their activation of caspase 3 while not affecting their differentiation. Neurosci. Lett. 2017-12-24 [PMID: 29278729] (Mouse)
• Pal-Ghosh S, Tadvalkar G, Stepp MA. Alterations in Corneal Sensory Nerves During Homeostasis, Aging, and After Injury in Mice Lacking the Heparan Sulfate Proteoglycan Syndecan-1. Invest. Ophthalmol. Vis. Sci. 2017-10-01 [PMID: 28973369] (ICC/IF, Mouse)
• Gu Y, Shi Y, Yang Q et al. miR-3074-5p Promotes the Apoptosis but Inhibits the Invasiveness of Human Extravillous Trophoblast-Derived HTR8/SVneo Cells In Vitro Reprod Sci 2017-01-01 [PMID: 28826362] (Human)
• Hara M, Kobayakawa K, Ohkawa Y et al. Interaction of reactive astrocytes with type I collagen induces astrocytic scar formation through the integrin-N-cadherin pathway after spinal cord injury. Nat. Med. 2017-06-19 [PMID: 28628111] (Mouse)
• Lemons K, Fu Z, Aoude I et al. Lack of TRPM5-Expressing Microvillous Cells in Mouse Main Olfactory Epithelium Leads to Impaired Odor-Evoked Responses and Olfactory-Guided Behavior in a Challenging Chemical Environment. eNeuro. 2017-06-14 [PMID: 28612045] (IF/IHC, Mouse)
• Fang Y, Huang X, Wan Y et al. Deficiency of TREK-1 potassium channel exacerbates secondary injury following spinal cord injury in mice. Journal of Neurochemistry 2017-03-15 [PMID: 28192611] (WB, ICC/IF, Mouse)
• Akins MR, Berk-Rauch HE, Kwan KY et al. Axonal ribosomes and mRNAs associate with fragile X granules in adult rodent and human brains Hum. Mol. Genet 2017-01-12 [PMID: 28082376]
• Lopes CD, Goncalves NP, Gomes CP et al. BDNF gene delivery mediated by neuron-targeted nanoparticles is neuroprotective in peripheral nerve injury. Biomaterials. 2016-12-24 [PMID: 28081461] (ICC/IF)
  
  Details:
  This citation used the Alexa Fluor 488 version of this antibody.
• Cheng N, Jiao S, Gumaste A et al. APP Overexpression Causes Abeta-independent Neuronal Death through Intrinsic Apoptosis Pathway eneuro 2016-07-08 [PMID: 27517085] (IF/IHC)
• Ku JM, Park K, Lee JH et al. Discovery, Optimization and Biological Evaluation of Sulfonamidoacetamides as an Inducer of Axon Regeneration J. Med. Chem. 2016-03-23 [PMID: 27007292] (IHC-Fr, Rat)
• Ueha R, Ueha S, Sakamoto T et al. Cigarette Smoke Delays Regeneration of the Olfactory Epithelium in Mice. Neurotox Res 2016-08-01 [PMID: 27003941] (Mouse)
• de Souza CF, Nivison-Smith L, Christie DL et al. Macromolecular markers in normal human retina and applications to human retinal disease. Exp. Eye Res. 2016-01-06 [PMID: 26769220] (IHC-Fr, Human)
• Janmaat CJ, de Rooij KE, Locher H et al. Human Dermal Fibroblasts Demonstrate Positive Immunostaining for Neuron- and Glia- Specific Proteins. PLoS One 2015-12-17 [PMID: 26678612] (IF/IHC)
• Ceber M, Sener U, Mihmanli A et al. The relationship between changes in the expression of growth associated protein-43 and functional recovery of the injured inferior alveolar nerve following transection without repair in adult rats. J Craniomaxillofac Surg 2015-08-28 [PMID: 26421471] (IHC-P, Rat)
• Nakano H, Iida Y, Suzuki M et al. Activating transcription factor 5 (ATF5) is essential for the maturation and survival of mouse basal vomeronasal sensory neurons. Cell Tissue Res. 2015-09-22 [PMID: 26395637] (IHC-Fr, Mouse)
• Smolek T, Madari A, Farbakova J et al. Tau hyperphosphorylation in synaptosomes and neuroinflammation are associated with canine cognitive impairment. J. Comp. Neurol. 2015-08-03 [PMID: 26239295] (WB, Canine)
• Ibrahim D, Taniguchi K, Yamamoto Y. Immunohistochemical and lectin histochemical studies on the developing olfactory organs of fetal camel. Microsc. Res. Tech. 2015-05-06 [PMID: 25950169] (IHC-P)
  
  Details:
  GAP43 antibody was used for IHC-P analysis of camel fetuses of several developmental stages and the staining was performed on vomeronasal organ (VNO) and the olfactory epithelium (OE) tissues. This antibody alongwith two others failed to detect camel's GAP45 in IHC-P assay which is correct in accordance with this antibody's immunogen sequence homology. NB300-143 is generated against AA216-226 of Rat/Mouse's Neuromodulin / Gap43 which does not show any sequence homology with camel's Neuromodulin.
• Rousselle SD, Brants IK, Sakaoka A et al. Neuromatous regeneration as a nerve response after catheter-based renal denervation therapy in a large animal model: immunohistochemical study. Circ Cardiovasc Interv. 2015-05-01 [PMID: 25940523] (IHC-P, Porcine)
• Baldo G, Lorenzini DM, Santos DS et al. Shotgun proteomics reveals possible mechanisms for cognitive impairment in Mucopolysaccharidosis I mice. Mol. Genet. Metab. 2014-12-13 [PMID: 25541102] (WB, Mouse)
• Pelletier SJ, LagaceM, St-Amour I et al. The morphological and molecular changes of brain cells exposed to direct current electric field stimulation. Int. J. Neuropsychopharmacol. 2014-12-07 [PMID: 25522422] (WB, Mouse)
• Zechel Sabrina, Zajac Pawel, Lonnerberg Peter et al. Topographical transcriptome mapping of the mouse medial ganglionic eminence by spatially resolved RNA-seq. Genome Biol. 2014-01-01 [PMID: 25344199] (IF/IHC, Mouse)
• Hong Jy, Lee Sh, Lee Sc et al. Therapeutic Potential of Induced neural Stem Cells for Spinal Cord Injury. J. Biol. Chem. 2014-10-06 [PMID: 25294882] (IF/IHC, Rat)
• Nakamuta S, Nakamuta N, Yamamoto Y et al. Transient Appearance of the Epithelial Invagination in the Olfactory Pit of Chick Embryos. J Vet. Med. Sci. 2014-09-18 [PMID: 25231436]
• Smith TD, Muchlinski MN, Bhatnagar KP et al. The vomeronasal organ of Lemur catta. Am. J Primatol. 2014-09-12 [PMID: 25220179]
• Garrett EC, Dennis JC, Bhatnagar KP et al. The vomeronasal complex of nocturnal strepsirhines and implications for the ancestral condition in primates. Anat Rec (Hoboken) 2013-12-01 [PMID: 24249398] (IHC-P, Primate)
• Ambjorn M, Dubreuil V, Miozzo F et al. A Loss-of-Function Screen for Phosphatases that Regulate Neurite Outgrowth Identifies PTPN12 as a Negative Regulator of TrkB Tyrosine Phosphorylation. PLoS One 2013-06-13 [PMID: 23785422] (WB, Human)
• Marks C, Belluscio L, Ibanez CF. Critical Role of GFRalpha1 in the Development and Function of the Main Olfactory System J Neurosci 2012-11-28 [PMID: 23197722] (IF/IHC, ICC/IF, Mouse)
• Ray A, Treloar HB. IgSF8: A developmentally and functionally regulated cell adhesion molecule in olfactory sensory neuron axons and synapses Mol Cell Neurosci 2012-06-09 [PMID: 22687584] (ICC/IF, IF/IHC, Mouse)
• de Souza CF, Kalloniatis M, Christie DL et al. Creatine transporter immunolocalization in aged human and detached retinas. Invest Ophthalmol Vis Sci 2012-04-01 [PMID: 22410560] (Human)
• Bauder AR, Ferguson TA. Reproducible mouse sciatic nerve crush and subsequent assessment of regeneration by whole mount muscle analysis J Vis Exp 2012-02-01 [PMID: 22395197] (ICC/IF, Mouse)
• Cheng N, Cai H, Belluscio L. In Vivo Olfactory Model of APP-Induced Neurodegeneration Reveals a Reversible Cell-Autonomous Function. J Neurosci;31(39):13699-13704. 2011-09-28 [PMID: 21957232] (ICC/IF, Human)
• Cheng K, Bai L, Belluscio L. Fas-associated factor 1 as a regulator of olfactory axon guidance. J Neurosci 1731(33):11905-11913. 2011-08-01 [PMID: 21849551] (IHC-Fr, Mouse)
• Murakami M, Ito H, Hagiwara K et al. Sphingosine kinase 1/S1P pathway involvement in the GDNF-induced GAP43 transcription J Cell Biochem 2011-11-01 [PMID: 21769916] (WB, Human)
• Grasselli et al. Impaired Sprouting and Axonal Atrophy in Cerebellar Climbing Fibres following In Vivo Silencing of the Growth-Associated Protein GAP-43. PLoS ONE. 2011-06-10 [PMID: 21695168] (WB, IF/IHC, Rat)
• Pluznick JL, Rodriguez-Gil DJ, Hull M et al. Renal cystic disease proteins play critical roles in the organization of the olfactory epithelium PLoS One 2011-01-01 [PMID: 21614130] (IF/IHC, ICC/IF, IHC-Fr, Mouse, Rat)
• Miller AM, Treloar HB, Greer CA. Composition of the migratory mass during development of the olfactory nerve. J Comp Neurol 518(24):4825-41. 2010-12-15 [PMID: 21031554] (WB, IF/IHC, Mouse)
• Rodriguez-Gil DJ, Greer CA. Wnt/Frizzled family members mediate olfactory sensory neuron axon extension. J Comp Neurol 511(3):301-17. 2008-11-20 [PMID: 18803244] (IHC-Fr, Mouse)
• Osterfield M, Egelund R, Young LM, Flanagan JG. Interaction of amyloid precursor protein with contactins and NgCAM in the retinotectal system. Development 135(6):1189-99. 2008-03-01 [PMID: 18272596] (IF/IHC, Chicken)
• Graham JB, Neubauer D, Xue QS et al. Chondroitinase applied to peripheral nerve repair averts retrograde axonal regeneration Exp Neurol. 2007-01-01 [PMID: 16970940] (IHC-Fr, Rat)
• Wu M, Wallace MR, Muir D et al. Tumorigenic properties of neurofibromin-deficient Schwann cells in culture and as syngrafts in Nf1 knockout mice. J Neurosci Res. 2005-11-01 [PMID: 16180234] (ICC/IF, Mouse)
• Teo, J-L et al. Specific inhibition of CBP/beta-catenin interaction rescues defects in neuronal differentiation caused by a presenilin-1 mutation. Proc Natl Acad Sci U S A 102(34):12171-6. 2005-08-23 [PMID: 16093313] (ICC/IF, Rat)
• Krekoski, C et al. Metalloproteinase-dependent predegeneration in vitro enhances axonal regeneration within acellular peripheral nerve grafts. J Neurosci 22(23):10408-15. 2002-12-01 [PMID: 12451140] (ICC/IF, Rat)
• Berwanger, B et al. Loss of a FYN-regulated differentiation and growth arrest pathway in advanced stage neuroblastoma. Cancer Cell 2(5):377-86. 2002-11-01 [PMID: 12450793] (WB, ICC/IF, Human)
• Zuo, J et al. Regeneration of axons after nerve transection repair is enhanced by degradation of chondroitin sulfate proteoglycan. Exp Neurol 176(1):221-8. 2002-07-01 [PMID: 12093099] (WB, ICC/IF, Rat)
• Krekoski, C et al. Axonal regeneration into acellular nerve grafts is enhanced by degradation of chondroitin sulfate proteoglycan. J Neurosci 21(16):6206-13. 2001-08-15 [PMID: 11487643] (ICC/IF, Rat)

Review for GAP-43 Antibody (NB300-143) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Bryan Tinsley IHC-P Mouse 11/07/2013

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: E15.5 Mouse
• Species: Mouse
• Lot: K-1

Bioinformatics

• Gene Symbol: GAP43