GAP-43 Antibody

Images

 
Western Blot: GAP-43 Antibody [NB300-143] - Western blots of homogenate of cow cerebellum stained with RPCA-GAP-43. A prominent band running at ~43kDa represents the full length GAP-43.
Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NB300-143] - Rat E18 mixed neuron/glia cultures with rabbit GAP43 (red) and 5B10, mouse monoclonal to MAP-tau (green).
Immunohistochemistry-Paraffin: GAP-43 Antibody [NB300-143] - Review image from confirmed customer on mouse E15.5 paraffin sections.
Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NB300-143] - Immunofluorescence of GAP-43 (green), a molecular marker of neurite outgrowth, demonstrates intense staining in overexpressing wild-type PS-1 (E) ...read more
Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NB300-143] - Mixed neuron-glial cultures stained with RPCA-GAP43 (red), blue is DNA staining.
Immunohistochemistry: GAP-43 Antibody [NB300-143] - Region CA1-CA4 of the hippocampus showing strong GAP-43 staining in cell processes and neutropil using NB300-143.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ca, Ch, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

GAP-43 Antibody Summary

Immunogen
C-terminal peptide of rat and mouse GAP43, which is KEDPEADQEHA, with an N-terminal Cys added to allow chemical coupling to KLH carrier protein.
Localization
Neuronal Marker, developing and regenerating axons and growth cone.
Marker
Neuronal Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GAP43
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Frozen 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
This GAP43 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded and frozen sections and Western blot, where it recognizes a band at 43 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB300-143 in the following applications:

Publications
Read Publications using
NB300-143 in the following applications:

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 25940523). Canine reactivity reported in scientific literature (PMID: 26239295)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GAP-43 Antibody

  • Axonal membrane protein GAP-43
  • B-50
  • Basp2
  • calmodulin-binding protein P-57
  • GAP43
  • GAP-43
  • growth associated protein 43
  • Growth-associated protein 43
  • nerve growth-related peptide GAP43
  • Neural phosphoprotein B-50
  • Neuromodulin
  • neuron growth-associated protein 43
  • PP46
  • protein F1

Background

GAP43 (growth associated protein 43, also called B-50, PP46, calmodulin-binding protein P-57, neuromodulin, neuron growth-associated protein 43, protein F1) is a major component of motile "growth cones" that form the tips of elongating axons as well as the synaptic plasma membranes. GAP43 is associated with nerve growth process and plays a key role in axonal as well as dendritic filopodia induction. It has been detected in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN, and binds calmodulin with superior affinity in the absence of Ca2+ than in its presence. Intracellular Ca2+ regulates PKC mediated phosphorylation of GAP43 and affects GAP43's ability to bind calmodulin. GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex. Aberrant expression of GAP43 has been seen in patients diagnosed with schizophrenia and Alzheimer's disease. Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 was independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual SDS-PAGE mobility properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14 kDa (to perform such calculations yourself see this link). GAP43 is one of many highly negatively charged extended molecules which lack well defined tertiary structure and contain few hydrophobic residues and which run anomalously on SDS-PAGE. Other examples are CAP23, MARCKS, microtubule associated proteins MAP2, tau and the Neurofilament subunits. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GAP-43 Antibody (NB300-143)(44)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Canine, Chicken, Porcine, Primate.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(14)
IHC
(11)
IHC-Fr
(7)
IHC-P
(4)
WB
(10)
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Human
(7)
Mouse
(17)
Rat
(10)
Canine
(1)
Chicken
(1)
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(1)
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Showing Publications 1 - 10 of 44. Show All 44 Publications.
Publications using NB300-143 Applications Species
Pal-Ghosh S, Tadvalkar G, Stepp MA. Alterations in Corneal Sensory Nerves During Homeostasis, Aging, and After Injury in Mice Lacking the Heparan Sulfate Proteoglycan Syndecan-1. Invest. Ophthalmol. Vis. Sci. 2017 Oct 01 [PMID: 28973369] (ICC/IF, Mouse) ICC/IF Mouse
Gu Y, Shi Y, Yang Q et al. miR-3074-5p Promotes the Apoptosis but Inhibits the Invasiveness of Human Extravillous Trophoblast-Derived HTR8/SVneo Cells In Vitro Reprod Sci 2017 Jan 01 [PMID: 28826362] (Human) Human
Hara M, Kobayakawa K, Ohkawa Y et al. Interaction of reactive astrocytes with type I collagen induces astrocytic scar formation through the integrin-N-cadherin pathway after spinal cord injury. Nat. Med. 2017 Jun 19 [PMID: 28628111] (Mouse) Mouse
Lemons K, Fu Z, Aoude I et al. Lack of TRPM5-Expressing Microvillous Cells in Mouse Main Olfactory Epithelium Leads to Impaired Odor-Evoked Responses and Olfactory-Guided Behavior in a Challenging Chemical Environment. eNeuro. 2017 Jun 14 [PMID: 28612045] (IHC, Mouse) IHC Mouse
Fang Y, Huang X, Wan Y et al. Deficiency of TREK-1 potassium channel exacerbates secondary injury following spinal cord injury in mice. Journal of Neurochemistry 2017 Mar 15 [PMID: 28192611] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Akins MR, Berk-Rauch HE, Kwan KY et al. Axonal ribosomes and mRNAs associate with fragile X granules in adult rodent and human brains Hum. Mol. Genet Jan 12 2017 [PMID: 28082376]
Lopes CD, Goncalves NP, Gomes CP et al. BDNF gene delivery mediated by neuron-targeted nanoparticles is neuroprotective in peripheral nerve injury. Biomaterials. 2016 Dec 24 [PMID: 28081461] (ICC/IF)

Details:
This citation used the Alexa Fluor 488 version of this antibody.
ICC/IF
Cheng N, Jiao S, Gumaste A et al. APP Overexpression Causes Abeta-independent Neuronal Death through Intrinsic Apoptosis Pathway eneuro 2016 Jul 08 [PMID: 27517085] (IHC) IHC
Ku JM, Park K, Lee JH et al. Discovery, Optimization and Biological Evaluation of Sulfonamidoacetamides as an Inducer of Axon Regeneration J. Med. Chem. Mar 23 2016 [PMID: 27007292] (IHC-Fr, Rat) IHC-Fr Rat
Ueha R, Ueha S, Sakamoto T et al. Cigarette Smoke Delays Regeneration of the Olfactory Epithelium in Mice. Neurotox Res. 2016 Mar 22 [PMID: 27003941] (Mouse) Mouse
Show All 44 Publications.

Review for GAP-43 Antibody (NB300-143) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB300-143:
Filter by Applications
IHC-P
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin GAP-43 NB300-143
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reviewed by:
Anony- mous
IHC-P Mouse 11/07/2013
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedE15.5 Mouse
SpeciesMouse
LotK-1
CommentsStrong signal even with this dilution. Almost no background. Excellent antibody.

Blocking

Blocking Details1% BSA in PBS-0.05% Tween20, 30 minutes

Primary Anitbody

Dilution Ratio1:1,000 in PBS-T +1% BSA, overnight at RT

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit 488, 2 hours at RT.
Secondary Manufacturer Cat#Alexa Fluor
Secondary Concentration1:500

Details

Detection NotesConfocal, Leica SP8. Neg control: omission of primary antibody.
Fixation Details4% paraformaldehyde overnight. Paraffin embedding. 5um sections.
Wash Description3X5min PBS-T

Comments

CommentsStrong signal even with this dilution. Almost no background. Excellent antibody.

Product General Protocols

View specific protocols for GAP-43 Antibody (NB300-143): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GAP-43 Antibody (NB300-143) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB300-143AF405
Alexa Fluor 488 Labeled NB300-143AF488
Alexa Fluor 647 Labeled NB300-143AF647
Alexa Fluor 700 Labeled NB300-143AF700
Biotin Labeled NB300-143B
DyLight 405 Labeled NB300-143V
DyLight 488 Labeled NB300-143G
DyLight 550 Labeled NB300-143R
DyLight 650 Labeled NB300-143C
FITC Labeled NB300-143F
HRP Labeled NB300-143H

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Blogs on GAP-43.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

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Recent Reviews

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Anony- mous
11/07/2013
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol GAP43
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