GAP-43 Antibody

Images

 
Immunohistochemistry-Paraffin: GAP-43 Antibody [NB300-143] - Review image from confirmed customer on mouse E15.5 paraffin sections.
Western Blot: GAP-43 Antibody [NB300-143] - Different tissue and cell lysates using rabbit pAb to GAP43, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] ...read more
Immunohistochemistry: GAP-43 Antibody [NB300-143] - Representative images of immunohistological staining (brown) of OMP-positive (OMP+) cells, GAP43+ immature ORNs. Tissue sections were counterstained with the nuclear ...read more
Immunohistochemistry-Paraffin: GAP-43 Antibody [NB300-143] - Posterior MOE sections at approximately the same region where EOG was recorded were obtained from control and Skn-1a-/- mice exposed to either water or ...read more
Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NB300-143] - Cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to GAP43, dilution 1:2,000 in green, and costained with mouse mAb to ...read more
Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NB300-143] - Rat E18 mixed neuron/glia cultures with rabbit GAP43 (red) and 5B10, mouse monoclonal to MAP-tau (green).
Western Blot: GAP-43 Antibody [NB300-143] - Western blots of homogenate of cow cerebellum stained with RPCA-GAP-43. A prominent band running at ~43kDa represents the full length GAP-43.
Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NB300-143] - Immunofluorescence of GAP-43 (green), a molecular marker of neurite outgrowth, demonstrates intense staining in overexpressing wild-type PS-1 (E) ...read more
Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NB300-143] - Mixed neuron-glial cultures stained with RPCA-GAP43 (red), blue is DNA staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Eq, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

GAP-43 Antibody Summary

Immunogen
C-terminal peptide of rodent GAP43, KEDPEADQEHA coupled to KLH.
Localization
Neuronal Marker, developing and regenerating axons and growth cone.
Marker
Neuronal Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GAP43
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Frozen 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
This GAP43 antibody is useful for Immunocytochemistry/Immunofluorescence, and Western blot, where it recognizes a band at 43 kDa. Use in IHC-P and IHC-Fr reported in scientific literature (PMID: 29950987 and 27007292 respectively). Use in FLOW reported in scientific literature (PMID: 21614130).
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB300-143 in the following applications:

Publications
Read Publications using
NB300-143 in the following applications:

Reactivity Notes

Reactivity to Canine, Chicken, and Primate reported in scientific literature (PMID: 30647968, 30819546, and 24249398 respectively). Drosophila reactivity reported in scientific literature (PMID: 30819546).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 50% glycerol (1:1)
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GAP-43 Antibody

  • Axonal membrane protein GAP-43
  • B-50
  • Basp2
  • calmodulin-binding protein P-57
  • GAP43
  • GAP-43
  • growth associated protein 43
  • Growth-associated protein 43
  • nerve growth-related peptide GAP43
  • Neural phosphoprotein B-50
  • Neuromodulin
  • neuron growth-associated protein 43
  • PP46
  • protein F1

Background

GAP43 (growth associated protein 43, also called B-50, PP46, calmodulin-binding protein P-57, neuromodulin, neuron growth-associated protein 43, protein F1) is a major component of motile "growth cones" that form the tips of elongating axons as well as the synaptic plasma membranes. GAP43 is associated with nerve growth process and plays a key role in axonal as well as dendritic filopodia induction. It has been detected in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN, and binds calmodulin with superior affinity in the absence of Ca2+ than in its presence. Intracellular Ca2+ regulates PKC mediated phosphorylation of GAP43 and affects GAP43's ability to bind calmodulin. GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex. Aberrant expression of GAP43 has been seen in patients diagnosed with schizophrenia and Alzheimer's disease. Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 was independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual SDS-PAGE mobility properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14 kDa. GAP43 is one of many highly negatively charged extended molecules which lack well defined tertiary structure and contain few hydrophobic residues and which run anomalously on SDS-PAGE. Other examples are CAP23, MARCKS, microtubule associated proteins MAP2, tau and the Neurofilament subunits. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-143
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Eq, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for GAP-43 Antibody (NB300-143)(74)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Canine, Chicken, Drosophila, Porcine, Primate.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-F, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(21)
IHC
(19)
IHC-F
(1)
IHC-Fr
(7)
IHC-P
(6)
WB
(18)
All Applications
Filter By Species
Human
(13)
Mouse
(31)
Rat
(12)
Canine
(2)
Chicken
(1)
Drosophila
(1)
Porcine
(3)
Primate
(1)
All Species
Showing Publications 1 - 10 of 74. Show All 74 Publications.
Publications using NB300-143 Applications Species
Cao W, Zhang C, Chen R et al. A Novel Cerebroprotein Hydrolysate, CH1, Ameliorates Chronic Focal Cerebral Ischemia Injury by Promoting White Matter Integrity via the Shh/Ptch-1/Gli-1 Signaling Pathway Neuropsychiatric disease and treatment Dec 23 2020 [PMID: 33380798] (WB, Mouse) WB Mouse
Zhang C, Cui L, He W et al. Dl-3-n-butylphthalide promotes neurite outgrowth of primary cortical neurons by Sonic Hedgehog signaling via upregulating Gap43 Experimental cell research Dec 6 2020 [PMID: 33296663] (ICC/IF, Mouse) ICC/IF Mouse
Collura KM, Niu J, Sanders SS et al. The palmitoyl acyltransferases ZDHHC5 and ZDHHC8 are uniquely present in DRG axons and control retrograde signaling via the Gp130/JAK/STAT3 pathway J. Biol. Chem. Sep 21 2020 [PMID: 32958558] (WB, Rat) WB Rat
Pal-Ghosh S, Tadvalkar G, Karpinski BA, Stepp MA Diurnal Control of Sensory Axon Growth and Shedding in the Mouse Cornea Invest. Ophthalmol. Vis. Sci. Sep 1 2020 [PMID: 32870244] (ICC/IF, Mouse) ICC/IF Mouse
Hasegawa-Ishii S, Imamura F, Nagayama S et Al. Differential effects of nasal inflammation and odor deprivation on layer-specific degeneration of the mouse olfactory bulb eNeuro Mar 20 2020 [PMID: 32220858] (Mouse) Mouse
Merino P, Diaz A, Torre ER, Yepes M Urokinase-type plasminogen activator (uPA) regulates the expression and function of growth-associated protein 43 (GAP-43) in the synapse J. Biol. Chem. Jan 10 2020 [PMID: 31819012] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Blaszkiewicz M, Willows JW, Dubois AL et al. Neuropathy and neural plasticity in the subcutaneous white adipose depot PLoS ONE Sep 11 2019 [PMID: 31509546] (WB, Mouse) WB Mouse
Zhou Y, Miles JR, Tavori H et al. Callosal septa express guidance cues and are paramedian guideposts for human corpus callosum development J. Anat. May 9 2019 [PMID: 31070791] (WB) WB
Liu Y, Hall B, Lwin N et Al. Tissue Responses and Wound Healing following Laser Scleral Microporation for Presbyopia Therapy Trans. Vis. Sci. Tech. Mar 9 2020 (IHC-F, IHC, Porcine) IHC-F, IHC Porcine
Niu J, Sanders SS, Jeong HK et al. Coupled Control of Distal Axon Integrity and Somal Responses to Axonal Damage by the Palmitoyl Acyltransferase ZDHHC17 Cell Rep Nov 17 2020 [PMID: 33207199]
Show All 74 Publications.

Review for GAP-43 Antibody (NB300-143) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB300-143:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin GAP-43 NB300-143
Enlarge
5
reviewed by:
Anony- mous
IHC-P Mouse 11/07/2013
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedE15.5 Mouse
SpeciesMouse
LotK-1
CommentsStrong signal even with this dilution. Almost no background. Excellent antibody.

Blocking

Blocking Details1% BSA in PBS-0.05% Tween20, 30 minutes

Primary Anitbody

Dilution Ratio1:1,000 in PBS-T +1% BSA, overnight at RT

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit 488, 2 hours at RT.
Secondary Manufacturer Cat#Alexa Fluor
Secondary Concentration1:500

Details

Detection NotesConfocal, Leica SP8. Neg control: omission of primary antibody.
Fixation Details4% paraformaldehyde overnight. Paraffin embedding. 5um sections.
Wash Description3X5min PBS-T

Comments

CommentsStrong signal even with this dilution. Almost no background. Excellent antibody.

Product General Protocols

View specific protocols for GAP-43 Antibody (NB300-143): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GAP-43 Antibody (NB300-143) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NB300-143AF405
Alexa Fluor 488 NB300-143AF488
Alexa Fluor 647 NB300-143AF647
Alexa Fluor 700 NB300-143AF700
Biotin NB300-143B
DyLight 405 NB300-143V
DyLight 488 NB300-143G
DyLight 550 NB300-143R
DyLight 650 NB300-143C
FITC NB300-143F
HRP NB300-143H
Janelia Fluor 549 NB300-143JF549
Janelia Fluor 646 NB300-143JF646

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Blogs on GAP-43.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

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Recent Reviews

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Anony- mous
11/07/2013
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol GAP43