SNAP25 Antibody

Images

 
Western Blot: SNAP25 Antibody [NBP1-33714] - Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-33714] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.
Western Blot: SNAP25 Antibody [NBP1-33714] - Analysis of SNAP25 on Hela cells using NBP1-33714 at a dilution of 1:1000. 30ug whole cell lysate loaded on 12% SDS PAGE gel.
Western Blot: SNAP25 Antibody [NBP1-33714] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.92 mg/ml

Order Details

SNAP25 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SNAP25. The exact sequence is proprietary.
Localization
Cytoplasm, perinuclear region, Cell membrane, Lipid-anchor, Cell junction, synapse, synaptosome
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SNAP25
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.92 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SNAP25 Antibody

  • bA416N4.2
  • dJ1068F16.2
  • FLJ23079
  • resistance to inhibitors of cholinesterase 4 homolog
  • RIC4
  • RIC-4
  • SEC9
  • SNAP25
  • SNAP-25SNAP
  • SUP
  • Super protein
  • Synaptosomal-associated 25 kDa protein
  • synaptosomal-associated protein 25
  • synaptosomal-associated protein, 25kD
  • synaptosomal-associated protein, 25kDa

Background

SNAP25 (Synaptosome-Associated Protein of 25 kDa) is a membrane bound, pre-synaptic nerve terminal protein that plays an essential role in synaptic vesicle fusion and exocytosis. It is part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. SNAP25 is involved in the molecular regulation of neurotransmitter release. It is thought to play an important role in the synaptic function of specific neuronal systems and it associates with proteins involved in vesicle docking and membrane fusion. SNAP25 exists as two alternatively spliced isoforms, SNAP25A and SNAP25B. These isoforms are differentially expressed in neurons and neuroendocrine cells and they differ by 9 amino acids in the central portion of the protein. This antibody is particularly valuable for immunocytochemical studies of the human brain due to its stability in formalin sections.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Blogs on SNAP25.

Characterizing Synaptophysin is "a Snap"
Synaptophysin is an integral membrane glycoprotein found within the small synaptic vesicles in brain and endocrine cells. Studies with synaptophysin antibodies show that it is one of the most abundant small vesicle proteins, constituting approximately...  Read full blog post.

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Bioinformatics

Gene Symbol SNAP25
Entrez