Fyn Antibody (FYN-01)

Immunocytochemistry/Immunofluorescence: Fyn Antibody (FYN-01) [NB500-517] - Staining of Fyn in murine transformed fibroblasts using anti-Fyn (FYN-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).

Immunocytochemistry/Immunofluorescence: Fyn Antibody (FYN-01) [NB500-517] - Staining of Fyn in human primary fibroblasts using anti-Fyn (FYN-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Immunocytochemistry/Immunofluorescence: Fyn Antibody (FYN-01) [NB500-517] - Staining of Fyn in human HeLa cell line using anti-Fyn (FYN-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Immunoprecipitation: Fyn Antibody (FYN-01) [NB500-517] - Immunoprecipitation of Fyn from the lysate of T cells isolated from fresh buffy coats. Western blot was immunostained with anti-Fyn (FYN-01). Lane 1: original lysate of T cells Lane 2-3: Immunoprecipitated material eluted from affinity sorbent (FYN-01 coupled to Sepharose beads). Lanes differ in amount of T cell lysate loaded on the immunosorbent.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IHC-P, IP
• Clone: FYN-01
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Fyn Antibody (FYN-01) Summary

• Immunogen: Bacterial expressed recombinant fragment, corresponding to amino acids 7-176 of Human Fyn.
• Localization: Cytoplasmic
• Specificity: The reacts with p59fyn (Fyn), a non receptor protein tyrosine kinase (59 kDa),which belongs to the Src family of cytoplasmic tyrosine kinases.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FYN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:50
• Application Notes: Immunoprecipitation - Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer; lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1% laurylmaltosid (w/v), 50 mM NaF) Application note: The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Fyn Knockout 293T Cell Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Fyn Antibody (FYN-01)

• EC 2.7.10
• EC 2.7.10.2
• FYN oncogene related to SRC, FGR, YES
• Fyn
• MGC45350
• OKT3-induced calcium influx regulator
• p59-Fyn
• protein-tyrosine kinase fyn
• Proto-oncogene c-Fyn
• Proto-oncogene Syn
• proto-oncogene tyrosine-protein kinase fyn
• SLK
• SLKc-syn protooncogene
• src/yes-related novel
• Src-like kinase
• SYN
• tyrosine kinase p59fyn(T)
• tyrosine-protein kinase Fyn

Background

Fyn is a ubiquitously expressed Src-family protein tyrosine kinase with important roles e.g. in immune and nervous system. It regulates N-methyl-D-aspartate (NMDA) receptor functions, thus affecting various brain functions, and even many of its other substrates are important for neural migration, synaptic plasticity, oligodendrocyte differentiation, and axon growth and guidance. In immune system Fyn namely regulates the commitment of T cells to activation, is important in T cell anergy induction, promotes mast cell chemotaxis and reorganization of cytoskeleton and participates in mast cell activation. Fyn is also involved in embryonic stem cell growth and differentiation, associates with tubulin and may play roles in mitotic spindle formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fyn Antibody (NB500-517)(4)

Publications using NB500-517

• Grossman H, Har-Paz E, Gindi N et al. Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments. Sci Rep May 22 2017 [PMID: 28533542] (WB)
• Hu H, Takano N, Xiang L et al. Hypoxia-inducible factors enhance glutamate signaling in cancer cells. Oncotarget 2014 Oct 15 [PMID: 25326682] (WB, Human)
• Taniguchi K, Xia L, Goldberg HJ et al. Inhibition of Src Kinase Blocks High Glucose-Induced EGFR Transactivation and Collagen Synthesis in Mesangial Cells and Prevents Diabetic Nephropathy in Mice. Diabetes. 2013 Aug 13 [PMID: 23942551]
• Jia R, Pan Q, Ding S et al. The N-terminal region of IFITM3 modulates its antiviral activity through regulating IFITM3 cellular localization J Virol 2012 Oct 10 [PMID: 23055554] (WB, ICC/IF, Human)

Review for Fyn Antibody (NB500-517) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous WB Human 10/04/2015

Summary

• Application: Western Blot
• Sample Tested: Human cancer cell whole cell lysate
• Species: Human

Blocking

• Blocking Details: Blocking with 5% non-fat milk for 30 min

Primary Anitbody

• Dilution Ratio: 1:1000, overnight

Secondary Antibody

• Secondary Description: Sheep anti-mouse IgG, HRP
• Secondary Manufacturer Cat#: GE Healthcare, NA931V
• Secondary Concentration: 1:2000

Details

• Detection Notes: Regular Western blot Protocol

Bioinformatics

• Gene Symbol: FYN
• Entrez:
  • 2534(Human)
  • 14360(Mouse)