Chromogranin A Antibody - BSA Free

Images

 
Immunohistochemistry-Paraffin: Chromogranin A Antibody [NB120-15160] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse pancreas using Chromogranin A antibody at a dilution of 1:500. The signal ...read more
Immunohistochemistry: Chromogranin A Antibody [NB120-15160] - IHC-P analysis fo human pancreas tissue showing specific staining of Chromogranin A in the Islets of Langerhans (dark brown staining).
Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody [NB120-15160] - The frequency of chromogranin A positive hormone-negative (CPHN) cells decreases with age. Representative pancreatic sections from fetal ...read more
Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody [NB120-15160] - SH-SY5Y cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more
Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody [NB120-15160] - Mouse pancreas cryosections stained with insulin (DAKO, Green) and Chromogranin A (NB120-15160; 1:500; White)
Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody [NB120-15160] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Chromogranin A Antibody - BSA Free Summary

Immunogen
Recombinant full length protein (Human).
Localization
Cytoplasmic, Vesicles, Secreted
Specificity
Chromogranin A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHGA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot
Application Notes
Use in Western Blot reported in scientific literature (PMID:32818433)IHC-P: Use at a dilution range of 1:500-1:1000. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. IF and ICC use reported in the literature (PMID: 22114116). Note:- Optimal dilutions/concentrations should be determined by the end user.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Chromogranin A Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 5
using
NB120-15160 in the following applications:

Publications
Read Publications using
NB120-15160 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27472443).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Chromogranin A Antibody - BSA Free

  • betagranin (N-terminal fragment of chromogranin A)
  • Betagranin
  • Catestatin
  • CGA
  • CHGA
  • Chromofungin
  • chromogranin A (parathyroid secretory protein 1)
  • Chromogranin A
  • chromogranin-A
  • Pancreastatin
  • Parastatin
  • Parathyroid Secretory Protein 1
  • Pituitary Secretory Protein I
  • SP-I

Background

Chromogranin A (a protein of 439 amino acids which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Chromogranin A Antibody (NB120-15160)(23)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(3)
IHC
(6)
IHC-Fr
(2)
IHC-P
(8)
WB
(1)
All Applications
Filter By Species
Human
(9)
Mouse
(9)
Rat
(1)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NB120-15160 Applications Species
Kopacz A, Kloska D, Klimczyk D et al. Nrf2 Transcriptional Activity Governs Intestine Development International journal of molecular sciences May 31 2022 [PMID: 35682851] (IHC-Fr, Mouse) IHC-Fr Mouse
Sriha J, Louis-Brennetot C, Pierre-Eugene C et al. BET and CDK Inhibition Reveal Differences in the Proliferation Control of Sympathetic Ganglion Neuroblasts and Adrenal Chromaffin Cells Cancers Jun 1 2022 [PMID: 35681734] (ICC/IF, Mouse) ICC/IF Mouse
Pandey U, Aich P Postnatal intestinal mucosa and gut microbial composition develop hand in hand: A mouse study Biomedical journal Mar 16 2022 [PMID: 35306225] (IHC-Fr, Mouse) IHC-Fr Mouse
Kuo W, Zuo L, Odenwald M Et al. The tight junction protein ZO-1 is dispensable for barrier function but critical for effective mucosal repair Gastroenterology Aug 1 2021 [PMID: 34478742]
Wilson S, Mayo M, Melim T et al. Optimized Culture Conditions for Improved Growth and Functional Differentiation of Mouse and Human Colon Organoids Frontiers in Immunology Feb 12 2021 [PMID: 33643277] (IHC, Mouse) IHC Mouse
Lin L, Petralia RS, Lake R et al. A novel structure associated with aging is augmented in the DPP6-KO mouse brain Acta Neuropathol Commun Nov 23 2020 [PMID: 33225987] (IHC, Mouse)

Details:
DPP6-KO and wild type mice hippocampi were analyzed via immunohistochemistry
IHC Mouse
Miao Y, Ha A, de Lau W et al. Next-Generation Surrogate Wnts Support Organoid Growth and Deconvolute Frizzled Pleiotropy In Vivo Cell Stem Cell Aug 14 2020 [PMID: 32818433] (WB, Human) WB Human
Cohen MA, Zhang S, Sengupta S et al. Formation of Human Neuroblastoma in Mouse-Human Neural Crest Chimeras Cell Stem Cell Feb 27 2020 [PMID: 32142683] (IHC, Mouse) IHC Mouse
Muthupalani S, Ge Z, Joy J et al. Muc5ac null mice are predisposed to spontaneous gastric antro-pyloric hyperplasia and adenomas coupled with attenuated H. pylori-induced corpus mucous metaplasia Lab. Invest. Aug 9 2019 [PMID: 31399638] (IHC-P, Mouse) IHC-P Mouse
Rosado-Olivieri E In Vitro Modeling of Human beta Cell Differentiation, Regeneration and Function Thesis (IHC, Human) IHC Human
Show All 23 Publications.

Reviews for Chromogranin A Antibody (NB120-15160) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NB120-15160:
Filter by Applications
IHC-Fr
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Chromogranin A NB120-15160
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Mouse 11/29/2016
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedPancreas tissue (cryosection)
SpeciesMouse
  5
reviewed by:
Steve Polyak
IF Mouse 05/07/2012
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse small intestine and colon
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Chromogranin A Antibody (NB120-15160). (Showing 1 - 1 of 1 FAQs).

  1. I have following question about antibodies against Chromogranin A. Your products list the species but canine is not included. Does it mean that the products cannot be used on canine tissues?
    • When we do not list a species, it means we have not tested an antibody to work with those samples. If we test an antibody and find that it does not work, we will list the species with a minus sign. Unfortunately, we haven't tried these abs on canine tissues and they may work, but we cannot guarantee it. If you would like to purchase one to try, you will be eligible for the Innovators Reward Program.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Verified Customer
11/29/2016
Application: IHC-Fr
Species: Mouse

 
Steve Polyak
05/07/2012
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol CHGA
Entrez
Uniprot