Chromogranin A Antibody

Images

 
Immunohistochemistry-Paraffin: Chromogranin A Antibody [NB120-15160] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse pancreas using Chromogranin A antibody at a dilution of 1:500. The signal ...read more
Immunohistochemistry: Chromogranin A Antibody [NB120-15160] - IHC-P analysis fo human pancreas tissue showing specific staining of Chromogranin A in the Islets of Langerhans (dark brown staining).
Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody [NB120-15160] - SH-SY5Y cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Chromogranin A Antibody Summary

Immunogen
Recombinant full length protein (Human).
Localization
Cytoplasmic, Vesicles, Secreted
Specificity
Chromogranin A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHGA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
IHC-P: Use at a dilution range of 1:500-1:1000. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. IF and ICC use reported in the literature (PMID: 22114116). Note:- Optimal dilutions/concentrations should be determined by the end user.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Chromogranin A Lysate (NBL1-09149)
Reviewed Applications
Read 2 Reviews rated 5
using
NB120-15160 in the following applications:

Publications
Read Publications using
NB120-15160 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27472443).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.6) and 1.0% BSA
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Chromogranin A Antibody

  • betagranin (N-terminal fragment of chromogranin A)
  • Betagranin
  • Catestatin
  • CGA
  • CHGA
  • Chromofungin
  • chromogranin A (parathyroid secretory protein 1)
  • Chromogranin A
  • chromogranin-A
  • Pancreastatin
  • Parastatin
  • Parathyroid Secretory Protein 1
  • Pituitary Secretory Protein I
  • SP-I

Background

Chromogranin A (a protein of 439 amino acids which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P

Publications for Chromogranin A Antibody (NB120-15160)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: IHC, IHC-P.


Filter By Application
IHC
(1)
IHC-P
(6)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
Rat
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB120-15160 Applications Species
Md Moin AS, Cory M, Ong A et al. Pancreatic Nonhormone Expressing Endocrine Cells in Children With Type 1 Diabetes J Endocr Soc 2017 May 01 [PMID: 28782056] (IHC-P, Human) IHC-P Human
Tuysuz N, van Bloois L, van den Brink S et al. Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells. Nat Commun. Mar 6 2017 [PMID: 28262686] (IHC-P, Human) IHC-P Human
Saleh Md Moin A, Dhawan S, Cory M et al. Increased frequency of hormone negative and polyhormonal endocrine cells in lean individuals with type 2 diabetes. J. Clin. Endocrinol. Metab. Jul 29 2016 [PMID: 27472443] (IHC, Rat) IHC Rat
Md Moin AS, Dhawan S, Shieh C et al. Increased hormone-negative endocrine cells in the pancreas in type 1 diabetes. J. Clin. Endocrinol. Metab. Jun 14 2016 [PMID: 27300574] (IHC-P) IHC-P
Helman A, Klochendler A, Azazmeh N, Gabai Y. p16Ink4a-induced senescence of pancreatic beta cells enhances insulin secretion. Nature medicine. 2016 Mar 07 [PMID: 26950362] (IHC-P, Mouse)

Details:
Polarity Sensitive Indicator of Viability Apoptosis Microscopy Kit /CytoGLO pSIVA IANBD kit (IMGENEX IMG-6701K) was used for the analysis of apoptosis in murine ovarian carcinoma (MOVCAR) cells which were subjected or not to 100?nM all trans retinoic acid, C3, and C4 treatment for 24?hrs.
IHC-P Mouse
Hubbard GK, Mutton LN, Khalili M et al. Combined MYC Activation and Pten Loss Are Sufficient to Create Genomic Instability and Lethal Metastatic Prostate Cancer. Cancer Res 2016 Jan 15 [PMID: 26554830]
Kwon SY, Bae YK, Gu MJ et al. Neuroendocrine differentiation correlates with hormone receptor expression and decreased survival in patients with invasive breast carcinoma. Histopathology. 2013 Oct 9 [PMID: 24117859] (IHC-P, Human) IHC-P Human
Park JK, Cho EJ, Ryu JK et al. Natural History and Malignant Risk Factors of Solid Pseudopapillary Tumors of the Pancreas Postgrad Med. 2013 Mar [PMID: 23816775] (IHC-P, Human) IHC-P Human
Polyak S, Mach A, Porvasnik S et al. Identification of Adeno-Associated Viral Vectors Suitable for Intestinal Gene Delivery and Modulation of Experimental Colitis. American Journal of Physiology. Gastrointestinal and Liver Physiology. [PMID: 22114116]

Reviews for Chromogranin A Antibody (NB120-15160) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.
We have 2 reviews tested in 2 application: IHC-Fr, IF.

Reviews using NB120-15160:
Filter by Applications
IHC-Fr
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Chromogranin A NB120-15160
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reviewed by:
Jason Spaeth
IHC-Fr Mouse 11/29/2016
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedPancreas tissue (cryosection)
SpeciesMouse
 
reviewed by:
Anonymous
IF Mouse 05/07/2012
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse small intestine and colon
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Chromogranin A Antibody (NB120-15160). (Showing 1 - 1 of 1 FAQs).

  1. I have following question about antibodies against Chromogranin A. Your products list the species but canine is not included. Does it mean that the products cannot be used on canine tissues?
    • When we do not list a species, it means we have not tested an antibody to work with those samples. If we test an antibody and find that it does not work, we will list the species with a minus sign. Unfortunately, we haven't tried these abs on canine tissues and they may work, but we cannot guarantee it. If you would like to purchase one to try, you will be eligible for the Innovators Reward Program.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Chromogranin A Antibody (NB120-15160)

Discover related pathways, diseases and genes to Chromogranin A Antibody (NB120-15160). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Chromogranin A.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Jason Spaeth
11/29/2016
Application: IHC-Fr
Species: Mouse

 
Anonymous
05/07/2012
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol CHGA
Entrez
Uniprot