Synaptophysin Antibody

Images

 
Western Blot: Synaptophysin Antibody [NBP2-25170] - Analysis of Synaptophysin in (A) human brain lysate and (B) mouse brain lysate.
Immunocytochemistry/ Immunofluorescence: Synaptophysin Antibody [NBP2-25170] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells ...read more
Immunohistochemistry: Synaptophysin Antibody [NBP2-25170] - Analysis of Synaptophysin in mouse brain.

Product Details

Summary
Reactivity Hu, Mu, Pm, Rt, Bv, SqSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Synaptophysin Antibody Summary

Immunogen
A synthetic peptide made to an C-terminal portion of the human Synaptophysin protein (between residues 230-313) [UniProt# P08247]
Localization
Golgi, cytoplasmic and membrane
Marker
Pre-synaptic marker
Predicted Species
Rat (95%), Bovine (96%), Squirrel (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SYP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:250
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100
Application Notes
Use in Immunohistochemistry-Frozen was reported in scientific literature (PMID: 31117227).
Control
Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publications using
NBP2-25170 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Synaptophysin Antibody

  • Major synaptic vesicle protein p38
  • MRX
  • MRXSYP
  • Synaptophysin
  • SYP

Background

Communication in the nervous system is dependent on the exocytosis of neurotransmitter-containing synaptic vesicles that release their contents at the synapses following membrane depolarization. Synaptophysin (SYP) is a major 38 kDa tetraspan membrane protein of small synaptic vesicles that plays an essential role in targeting synaptic vesicles to the presynaptic membrane. Synaptophysin directly interacts with the essential membrane fusion v-SNARE protein, synaptobrevin/VAMP2, and targets it to the presynaptic membrane (PMID: 14528015). Thus, synaptophysin is required for proper targeting of VAMP2 and synaptic vesicles. Synaptophysin is localized to the trans-Golgi cisternae and then accumulates to the presynaptic membrane in a microtubule dependent manner (PMID: 8227215).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Synaptophysin Antibody (NBP2-25170)(3)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Synaptophysin Antibody (NBP2-25170) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-25170AF350
Alexa Fluor 405 NBP2-25170AF405
Alexa Fluor 488 NBP2-25170AF488
Alexa Fluor 532 NBP2-25170AF532
Alexa Fluor 594 NBP2-25170AF594
Alexa Fluor 647 NBP2-25170AF647
Alexa Fluor 700 NBP2-25170AF700
Alexa Fluor 750 NBP2-25170AF750
Biotin NBP2-25170B
DyLight 350 NBP2-25170UV
DyLight 405 NBP2-25170V
DyLight 488 NBP2-25170G
DyLight 550 NBP2-25170R
DyLight 594 NBP2-25170DL594
DyLight 650 NBP2-25170C
DyLight 680 NBP2-25170FR
DyLight 755 NBP2-25170IR
FITC NBP2-25170F
HRP NBP2-25170H
Janelia Fluor 549 NBP2-25170JF549
Janelia Fluor 646 NBP2-25170JF646

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Blogs on Synaptophysin.

Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
 By Jamshed Arslan Pharm.D.  Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...  Read full blog post.

Synaptophysin a Marker Protein in Neuroendocrine Cells
Synaptophysin a Marker Protein in Neuroendocrine Cells Synaptophysin is a major integral membrane glycoprotein of neuronal synaptic vesicles present in virtually all synapses and shows a high degree of evolutionary conservation across the mammals. Syn...  Read full blog post.

Characterizing Synaptophysin is "a Snap"
Synaptophysin is an integral membrane glycoprotein found within the small synaptic vesicles in brain and endocrine cells. Studies with synaptophysin antibodies show that it is one of the most abundant small vesicle proteins, constituting approximately...  Read full blog post.

Synaptophysin and Dementing Disorders
 Synaptophysin (a presynaptic vesicle protein) is an integral membrane glycoprotein originally isolated from presynaptic vesicles of bovine neurons. Synaptophysin is found in all nerve terminals and synaptophysin measurements have been used to quantif...  Read full blog post.

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Bioinformatics

Gene Symbol SYP
Entrez
Uniprot