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Enolase 2/Neuron-specific Enolase Antibody

 (5 Publications)
 (2 Reviews)
 
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Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of different tissue and cell lysates using rabbit pAb to neuron specific enolase (NSE), NB110-58870, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH-3T3, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. A single band at about 47kDa corresponds to the NSE protein, seen only in extracts containing neurons ro neuronal lineage cells.
Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of different tissue and cell lysates using rabbit pAb to neuron specific enolase (NSE), NB110-58870, dilution 1:5,000 in green: [1] ...read more
Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of mixed cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to neuron specific enolase ...read more
Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Blot of rat spinal cord probed with rabbit antibody to NSE. The antibody stains a single sharp band corresponing to NSE at about 47kDa.
Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Western blot analysis of extracts from mouse brain using NSE antibody (NB110-58870, 1:100).
Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Shows the human embryonic kidney cells line 293, which express many neuronal proteins (1). The red channel shows ...read more
Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Confocal immunofluorescent analysis of 3T3 cells using NSE antibody (NB110-58870, 1:5). An Alexa Fluor 488-conjugated ...read more
Simple Western: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Simple Western lane view shows a specific band for Enolase 2/ Neuron specific enolase in 0.05 mg/ml of Human Brain lysate. This experiment was ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

100% Guarantee on every product

Enolase 2/Neuron-specific Enolase Antibody Summary

Immunogen
Recombinant full length human NSE purified from E. coli. [UniProt# P09104]
Marker
Neuronal Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ENO2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:500
  • Knockdown Validated
  • Simple Western 1:10000
  • Western Blot 1:2000
Application Notes
This NSE antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Enolase 2/Neuron-specific Enolase Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 5
using
NB110-58870 in the following applications:
Publications
Read Publications using
NB110-58870 in the following applications:

  • ICC/IF
    2 publications
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Supplied as serum
Preservative
5mM Sodium Azide
Purity
Unpurified

Alternate Names for Enolase 2/Neuron-specific Enolase Antibody

  • 2-phospho-D-glycerate hydrolyase
  • 2-phospho-D-glycerate hydro-lyase
  • EC 4.2.1.11
  • ENO2
  • enolase 2 (gamma, neuronal)
  • Enolase 2
  • gamma-Enolase
  • Neural enolase
  • neuron specific gamma enolase
  • neurone-specific enolase
  • Neuronspecific Enolase
  • Neuron-specific enolase
  • NSE

Background

NSE (neuron-specific enolase or enolase 2) is an enzyme which facilitates the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolytic pathway, and also the reverse reaction in gluconeogenic metabolism. It belongs to mammalian enolases ENO1, ENO2, and ENO3 (alternately known as enolase alpha, beta and gamma respectively) and these three enolases have different cell type specific expression patterns. NSE is found in relatively large amounts in neurons, PNS tissues and neuroendocrine cells, and is one of the most commonly employed biochemical markers of nervous tissue damage. Increased NSE levels in serum, CSF, or both have been reported in several neurological pathologies such as status epilepticus, Creutzfeldt-Jakob disease, acute ischemic stroke, head injury and hypoxic brain injury and Tuberculous Meningitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: GFAP Antibody [NB300-141] - Analysis of different tissue lysates using rabbit polyclonal antibody to GFAP, NB300-141, dilution 1:5000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Strong band at about 50 kDa corresponds to the major isotype of the GFAP protein. Smaller isotypes and proteolytic fragments of GFAP are also detected on the blot.
Western Blot: GFAP Antibody [NB300-141] - Analysis of Rat brain lysate. Antibody at 1:5000. Specific band running with an apparent SDS-PAGE molecular weight of ~50 kDa corresponds to rodent GFAP was observed.
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Western Blot: Synaptophysin Antibody [NB300-653] - Approximate MW of Synaptophysin is 39kDa.
Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - Rat brain, 1:100 dilution.
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Immunohistochemistry-Frozen: Vimentin Antibody [NB300-223] - 10 um Cryosections from PFA fixed murine brain acute slices stained with Vimentin antibody (1:600) and Alexa Fluor 488 anti chicken IgG. Antibody showed specific staining, also of blood vessels. IHC-Fr image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Human A7 cells. Cells were fixed with 10% formalin and permeabilized with 0.1% TritonX-100. After blocking, cells were incubated with primary antibody (1:600) at 4C O/N followed with Alexa Fluor 488 labeled secondary antibody and counterstained with DAPI and Phalloidin for nuclei and F-actin respectively. Imaged with Leica TCS SP5 confocal microscope. ICC/IF image submitted by a verified customer review.
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Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Chromogranin A Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - The frequency of chromogranin A positive hormone-negative (CPHN) cells decreases with age. Representative pancreatic sections from fetal (A) and infant-child (B-D) cases immunostained for Endocrine cocktail (insulin, glucagon, somatostatin, pancreatic polypeptide, and ghrelin) (white), chromogranin A (green), and DAPI (blue). Yellow arrows show CPHN cells. Scale bars, 50 um. Image collected and cropped by CiteAb from the following publication (https://www.frontiersin.org/article/10.3389/fendo.2018.00791/full), licensed under a CC-BY licence.
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Immunohistochemistry: VIP Antibody (1E8) [NBP2-46349] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: VIP Antibody (1E8) [NBP2-46349] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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MBP Antibody (12)
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Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717] - Staining of MBP in mouse spinal cord ventral white matter. Image from verified customer review.
Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717] - Staining of MBP in mouse brain corpus callosum. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: MBP Antibody (12) [NB600-717] - Histological and mRNA analysis of the inflammatory cytokines in the vehicle- or etifoxine-treated mice at onset of clinical symptoms. At day 10 p.i., drug treated animals showed significant differences in MBP staining. Animals treated with etifoxine showed an increase in retention of percentage of MBP coverage (*p = 0.001). Image collected and cropped by CiteAb from the following publication (http://embomolmed.embopress.org/cgi/doi/10.1002/emmm.201202124), licensed under a CC-BY licence.
(3 Reviews)
(29 Publications)
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Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, RI, WB
Enolase
2/Neuron-specific Eno ...
NB110-58870
Enolase 2/Neuron-specific Enolase Antibody
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, KD

Publications for Enolase 2/Neuron-specific Enolase Antibody (NB110-58870)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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(2)
WB
(2)
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(4)
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Reviews for Enolase 2/Neuron-specific Enolase Antibody (NB110-58870) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NB110-58870:
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(1)
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(1)
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(2)
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Images Ratings Applications Species Date Details
Immunocytochemistry Enolase 2/Neuron-specific Enolase NB110-58870
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reviewed by:
Verified Customer
 ICC Mouse 06/05/2014
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Summary

ApplicationImmunocytochemistry
SpeciesMouse
Western Blot Enolase 2/Neuron-specific Enolase NB110-58870
Enlarge 		5
reviewed by:
Verified Customer
 WB Mouse 06/05/2014
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Summary

ApplicationWestern Blot
SpeciesMouse

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FAQs for Enolase 2/Neuron-specific Enolase Antibody (NB110-58870) (0)

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Control Lysate(s)

Enolase 2/Neuron-specific Enolase Overexpression Lysate

Secondary Antibodies

 

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Immunocytochemistry Enolase 2/Neuron-specific Enolase NB110-58870
Enlarge
 Verified Customer
06/05/2014
Application: ICC
Species: Mouse
read this review
Western Blot Enolase 2/Neuron-specific Enolase NB110-58870
Enlarge
 Verified Customer
06/05/2014
Application: WB
Species: Mouse
read this review

Bioinformatics

Gene Symbol ENO2
Entrez
Uniprot