Enolase 2/Neuron-specific Enolase Antibody

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of different tissue and cell lysates using rabbit pAb to neuron specific enolase (NSE), NB110-58870, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH-3T3, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. A single band at about 47kDa corresponds to the NSE protein, seen only in extracts containing neurons ro neuronal lineage cells.

Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of mixed cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to neuron specific enolase (NSE), NB110-58870, dilution 1:500 in red, and costained with chicken pAb to GFAP, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. the NSE antibody labels protein expressed in neuronal cells, while the GFAP antibody stains intermediate filaments in astrocytic and certain other glial cells.

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Blot of rat spinal cord probed with rabbit antibody to NSE. The antibody stains a single sharp band corresponing to NSE at about 47kDa.

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Western blot analysis of extracts from mouse brain using NSE antibody (NB110-58870, 1:100).

Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Shows the human embryonic kidney cells line 293, which express many neuronal proteins (1). The red channel shows staining which recognizes all of these 293 cells. The green channels shows staining for another neuronal marker to UCHL1 using NB110-58869. This neuronal gene is apparently activated in a cell density dependent fashion and at this stage only a few cells express this protein. However all cells that express NSE also express UCHL1.

Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Confocal immunofluorescent analysis of 3T3 cells using NSE antibody (NB110-58870, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).

Simple Western: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Simple Western lane view shows a specific band for Enolase 2/ Neuron specific enolase in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Enolase 2/Neuron-specific Enolase Antibody Summary

• Immunogen: Recombinant full length human NSE purified from E. coli. [UniProt# P09104]
• Marker: Neuronal Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ENO2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry 1:500
  • Knockdown Validated
  • Simple Western 1:10000
  • Western Blot 1:2000
• Application Notes: This NSE antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in Human Brain lysate 0.05 mg/mL, separated by Size, antibody dilution of 1:10,000. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Enolase 2/Neuron-specific Enolase Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Supplied as serum
• Preservative: 0.035% Sodium Azide
• Purity: Unpurified

Alternate Names for Enolase 2/Neuron-specific Enolase Antibody

• 2-phospho-D-glycerate hydrolyase
• 2-phospho-D-glycerate hydro-lyase
• EC 4.2.1.11
• ENO2
• enolase 2 (gamma, neuronal)
• Enolase 2
• gamma-Enolase
• Neural enolase
• neuron specific gamma enolase
• neurone-specific enolase
• Neuronspecific Enolase
• Neuron-specific enolase
• NSE

Background

NSE (neuron-specific enolase or enolase 2) is an enzyme which facilitates the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolytic pathway, and also the reverse reaction in gluconeogenic metabolism. It belongs to mammalian enolases ENO1, ENO2, and ENO3 (alternately known as enolase alpha, beta and gamma respectively) and these three enolases have different cell type specific expression patterns. NSE is found in relatively large amounts in neurons, PNS tissues and neuroendocrine cells, and is one of the most commonly employed biochemical markers of nervous tissue damage. Increased NSE levels in serum, CSF, or both have been reported in several neurological pathologies such as status epilepticus, Creutzfeldt-Jakob disease, acute ischemic stroke, head injury and hypoxic brain injury and Tuberculous Meningitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Enolase 2/Neuron-specific Enolase Antibody (NB110-58870)(5)

Publications using NB110-58870

• Niture S, Tricoli L, Qi Q Et al. MicroRNA miR-99b-5p Targets mTOR/AR axis, Induces Autophagy, and Inhibits Prostate Cancer Cell Proliferation Tumour Biol 2022-07-11 [PMID: 35811549]
• Powers RM, Daza R, Koehler AE et al. Growth cone macropinocytosis of neurotrophin receptor and neuritogenesis are regulated by neuron navigator 1 Molecular biology of the cell 2022-06-01 [PMID: 35352947] (ICC/IF, Human)
  
  Details:
  Dilution used for ICC 1:250
• Gong W, Zhang S, Zong Y et al. hiPS Cell-Derived Neurons for High-Throughput Screening Methods Mol. Biol. 2019-05-25 [PMID: 31124122] (ICC/IF, Human)
• Modarai S, Gupta A, Opdenaker L et al. The anti-cancer effect of retinoic acid signaling in CRC occurs via decreased growth of ALDH+ colon cancer stem cells and increased differentiation of stem cells Otolaryngol Head Neck Surg 2018-10-16 [PMID: 30410666] (WB, Human)
• Jung Y, Cackowski FC, Yumoto K, Decker A. CXCL12gamma Promotes Development of Metastatic Castration Resistant Prostate Cancer by Induction of Cancer Stem Cell and Neuroendocrine Phenotypes. Cancer Res. 2018-02-05 [PMID: 29431639] (WB, Human)

Reviews for Enolase 2/Neuron-specific Enolase Antibody (NB110-58870) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Bryan Tinsley ICC Mouse 06/05/2014

Summary

• Application: Immunocytochemistry
• Species: Mouse

reviewed by: Bryan Tinsley WB Mouse 06/05/2014

Summary

• Application: Western Blot
• Species: Mouse

Bioinformatics

• Gene Symbol: ENO2
• Entrez:
  • 2026(Human)
  • 24334(Rat)
• Uniprot:
  • P09104()