Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of different tissue and cell lysates using rabbit pAb to neuron specific enolase (NSE), NB110-58870, dilution 1:5,000 in green:  ...read more
Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of mixed cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to neuron specific enolase ...read more
Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Blot of rat spinal cord probed with rabbit antibody to NSE. The antibody stains a single sharp band corresponing to NSE at about 47kDa.
Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Western blot analysis of extracts from mouse brain using NSE antibody (NB110-58870, 1:100).
Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Shows the human embryonic kidney cells line 293, which express many neuronal proteins (1). The red channel shows ...read more
Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Confocal immunofluorescent analysis of 3T3 cells using NSE antibody (NB110-58870, 1:5). An Alexa Fluor 488-conjugated ...read more
Simple Western: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Simple Western lane view shows a specific band for Enolase 2/ Neuron specific enolase in 0.05 mg/ml of Human Brain lysate. This experiment was ...read more
This NSE antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
47 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Supplied as serum
5mM Sodium Azide
Alternate Names for Enolase 2/Neuron-specific Enolase Antibody
enolase 2 (gamma, neuronal)
neuron specific gamma enolase
NSE (neuron-specific enolase or enolase 2) is an enzyme which facilitates the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolytic pathway, and also the reverse reaction in gluconeogenic metabolism. It belongs to mammalian enolases ENO1, ENO2, and ENO3 (alternately known as enolase alpha, beta and gamma respectively) and these three enolases have different cell type specific expression patterns. NSE is found in relatively large amounts in neurons, PNS tissues and neuroendocrine cells, and is one of the most commonly employed biochemical markers of nervous tissue damage. Increased NSE levels in serum, CSF, or both have been reported in several neurological pathologies such as status epilepticus, Creutzfeldt-Jakob disease, acute ischemic stroke, head injury and hypoxic brain injury and Tuberculous Meningitis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Modarai S, Gupta A, Opdenaker L et al. The anti-cancer effect of retinoic acid signaling in CRC occurs via decreased growth of ALDH+ colon cancer stem cells and increased differentiation of stem cells Oncotarget Oct 5 2018 (WB, Human)
Confocal immunofluorescent analysis of 3T3 cells using NSE antibody (NB110-58870, 1:5). An Alexa FluorÂ® 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa FluorÂ® 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).
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