Enolase 2/Neuron-specific Enolase Antibody

Images

 
Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of different tissue and cell lysates using rabbit pAb to neuron specific enolase (NSE), NB110-58870, dilution 1:5,000 in green: [1] ...read more
Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of mixed cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to neuron specific enolase ...read more
Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Blot of rat spinal cord probed with rabbit antibody to NSE. The antibody stains a single sharp band corresponing to NSE at about 47kDa.
Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Western blot analysis of extracts from mouse brain using NSE antibody (NB110-58870, 1:100).
Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Shows the human embryonic kidney cells line 293, which express many neuronal proteins (1). The red channel shows ...read more
Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Confocal immunofluorescent analysis of 3T3 cells using NSE antibody (NB110-58870, 1:5). An Alexa Fluor 488-conjugated ...read more
Simple Western: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Simple Western lane view shows a specific band for Enolase 2/ Neuron specific enolase in 0.05 mg/ml of Human Brain lysate. This experiment was ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Enolase 2/Neuron-specific Enolase Antibody Summary

Immunogen
Recombinant full length human NSE purified from E. coli. [UniProt# P09104]
Marker
Neuronal Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ENO2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Simple Western 1:10000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:500
Application Notes
This NSE antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Enolase 2/Neuron-specific Enolase Lysate (NBL1-10268)
Reviewed Applications
Read 2 Reviews rated 5
using
NB110-58870 in the following applications:

Publications
Read Publication using
NB110-58870 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
10mM Sodium Azide
Purity
Unpurified

Alternate Names for Enolase 2/Neuron-specific Enolase Antibody

  • 2-phospho-D-glycerate hydrolyase
  • 2-phospho-D-glycerate hydro-lyase
  • EC 4.2.1.11
  • ENO2
  • enolase 2 (gamma, neuronal)
  • Enolase 2
  • gamma-Enolase
  • Neural enolase
  • neuron specific gamma enolase
  • neurone-specific enolase
  • Neuronspecific Enolase
  • Neuron-specific enolase
  • NSE

Background

NSE (neuron-specific enolase or enolase 2) is an enzyme which facilitates the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolytic pathway, and also the reverse reaction in gluconeogenic metabolism. It belongs to mammalian enolases ENO1, ENO2, and ENO3 (alternately known as enolase alpha, beta and gamma respectively) and these three enolases have different cell type specific expression patterns. NSE is found in relatively large amounts in neurons, PNS tissues and neuroendocrine cells, and is one of the most commonly employed biochemical markers of nervous tissue damage. Increased NSE levels in serum, CSF, or both have been reported in several neurological pathologies such as status epilepticus, Creutzfeldt-Jakob disease, acute ischemic stroke, head injury and hypoxic brain injury and Tuberculous Meningitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC

Publications for Enolase 2/Neuron-specific Enolase Antibody (NB110-58870)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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(1)
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Reviews for Enolase 2/Neuron-specific Enolase Antibody (NB110-58870) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NB110-58870:
Filter by Applications
ICC
(1)
WB
(1)
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Mouse
(2)
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Images Ratings Applications Species Date Details
Immunocytochemistry Enolase 2/Neuron-specific Enolase NB110-58870
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reviewed by:
Anony- mous
ICC Mouse 06/05/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesMouse
Special ApplicationsConfocal immunofluorescent analysis of 3T3 cells using NSE antibody (NB110-58870, 1:5). An Alexa Fluor® 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor® 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).
FileView PDF
Western Blot Enolase 2/Neuron-specific Enolase NB110-58870
Enlarge
reviewed by:
Anony- mous
WB Mouse 06/05/2014
View

Summary

ApplicationWestern Blot
SpeciesMouse
Special ApplicationsWestern blot analysis of extracts from mouse brain using NSE antibody (NB110-58870, 1:100).
FileView PDF

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Enolase 2/Neuron-specific Enolase Antibody (NB110-58870) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

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Recent Reviews

5
5
2
4
0
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0
2
0
1
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Anony- mous
06/05/2014
Application: ICC
Species: Mouse

Anony- mous
06/05/2014
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol ENO2
Entrez
Uniprot