Enolase 2/Neuron-specific Enolase Antibody

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Western blot analysis of extracts from mouse brain using NSE antibody (NB110-58870, 1:100).

Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Confocal immunofluorescent analysis of 3T3 cells using NSE antibody (NB110-58870, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Blot of rat spinal cord probed with rabbit antibody to NSE. The antibody stains a single sharp band corresponing to NSE at about 47kDa.

Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Shows the human embryonic kidney cells line 293, which express many neuronal proteins (1). The red channel shows staining which recognizes all of these 293 cells. The green channels shows staining for another neuronal marker to UCHL1 using NB110-58869. This neuronal gene is apparently activated in a cell density dependent fashion and at this stage only a few cells express this protein. However all cells that express NSE also express UCHL1.

Simple Western: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Simple Western lane view shows a specific band for Enolase 2/ Neuron specific enolase in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Enolase 2/Neuron-specific Enolase Antibody Summary

• Immunogen: Recombinant full length human NSE purified from E. coli. [UniProt# P09104]
• Marker: Neuronal Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ENO2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
  • Simple Western 1:10000
  • Immunocytochemistry/Immunofluorescence 1:500
• Application Notes: This NSE antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Positive Control:

  Enolase 2/Neuron-specific Enolase Lysate (NBL1-10268)

Reactivity Notes

Mouse data from customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 10mM Sodium Azide
• Purity: Unpurified

Alternate Names for Enolase 2/Neuron-specific Enolase Antibody

• 2-phospho-D-glycerate hydrolyase
• 2-phospho-D-glycerate hydro-lyase
• EC 4.2.1.11
• ENO2
• enolase 2 (gamma, neuronal)
• Enolase 2
• gamma-Enolase
• Neural enolase
• neuron specific gamma enolase
• neurone-specific enolase
• Neuronspecific Enolase
• Neuron-specific enolase
• NSE

Background

NSE (neuron-specific enolase or enolase 2) is an enzyme which facilitates the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolytic pathway, and also the reverse reaction in gluconeogenic metabolism. It belongs to mammalian enolases ENO1, ENO2, and ENO3 (alternately known as enolase alpha, beta and gamma respectively) and these three enolases have different cell type specific expression patterns. NSE is found in relatively large amounts in neurons, PNS tissues and neuroendocrine cells, and is one of the most commonly employed biochemical markers of nervous tissue damage. Increased NSE levels in serum, CSF, or both have been reported in several neurological pathologies such as status epilepticus, Creutzfeldt-Jakob disease, acute ischemic stroke, head injury and hypoxic brain injury and Tuberculous Meningitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Enolase 2/Neuron-specific Enolase Antibody (NB110-58870)(1)

Publication using NB110-58870

• Jung Y, Cackowski FC, Yumoto K, Decker A. CXCL12gamma Promotes Development of Metastatic Castration Resistant Prostate Cancer by Induction of Cancer Stem Cell and Neuroendocrine Phenotypes. Cancer Res. 2018 Feb 05 [PMID: 29431639] (WB, Human)

Reviews for Enolase 2/Neuron-specific Enolase Antibody (NB110-58870) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Anony- mous ICC Mouse 06/05/2014

Summary

• Application: Immunocytochemistry
• Species: Mouse
• Special Applications: Confocal immunofluorescent analysis of 3T3 cells using NSE antibody (NB110-58870, 1:5). An Alexa Fluor® 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor® 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).
• File: View PDF

reviewed by: Anony- mous WB Mouse 06/05/2014

Summary

• Application: Western Blot
• Species: Mouse
• Special Applications: Western blot analysis of extracts from mouse brain using NSE antibody (NB110-58870, 1:100).
• File: View PDF

Bioinformatics

• Gene Symbol: ENO2
• Entrez:
  • 2026(Human)
  • 13807(Mouse)
  • 24334(Rat)
• Uniprot:
  • P09104()