Desmin Antibody

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Desmin was detected in immersion fixed frozen sections of mouse skeletal muscle using Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) at 10 µg/mL overnight at 4 °C. Tissue was stained ...read more
Desmin was detected in immersion fixed paraffin-embedded sections of human heart using 15 µg/mL Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Desmin Antibody Summary

Immunogen
E. coli-derived recombinant human Desmin
Val261-Leu470
Accession # P17661
Specificity
Detects human and mouse Desmin.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DES
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF3844 in the following application:

Publications
Read Publications using
AF3844 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Desmin Antibody

  • CMD1I
  • CMD1IFLJ41013
  • CSM1
  • CSM2
  • DES
  • Desmin
  • FLJ12025
  • FLJ39719
  • FLJ41793
  • intermediate filament protein

Background

Desmin is a 53 kDa, muscle-specific molecule that belongs to the intermediate filament superfamily of cytoskeletal proteins. Cytoplasmic Desmin homopolymers form stable filamentous networks that stabilize lateral alignments of myofibrils. Human Desmin is 470 amino acid (aa) peptide. It contains an N-terminal head segment (aa 1-84), multiple coiled regions (aa 109-412), and a tail region (aa 413-470). Single amino acid substitutions and three-amino acid deletions are associated with cardio‑skeletal myopathies. Most mutations affect filament assembly. Desmin is a highly conserved protein. Over the region used as immunogen, human Desmin is 99% identical to the corresponding mouse, canine, and porcine protein sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Desmin Antibody (AF3844)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 9 applications: ICC, IHC, IHC - Paraffin embedded, IHC Fresh, IHC OCT-embedded, IHC Paraffin-embedded, IHC-Fr, IHC-P, WB.


Filter By Application
ICC
(2)
IHC
(1)
IHC - Paraffin embedded
(2)
IHC Fresh
(1)
IHC OCT-embedded
(1)
IHC Paraffin-embedded
(1)
IHC-Fr
(1)
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Human
(7)
Mouse
(4)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF3844 Applications Species
C Szot, S Saha, XM Zhang, Z Zhu, MB Hilton, K Morris, S Seaman, JM Dunleavey, KS Hsu, GJ Yu, H Morris, DA Swing, DC Haines, Y Wang, J Hwang, Y Feng, D Welsch, G DeCrescenz, A Chaudhary, E Zudaire, DS Dimitrov, B St Croix Tumor stroma-targeted antibody-drug conjugate triggers localized anticancer drug release J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29863500] (IHC-P,, Human) IHC-P Human
HS Lee, J Choi, T Son, HJ Wee, SJ Bae, JH Seo, JH Park, SH Ryu, D Lee, MK Jang, E Yu, YH Chung, KW Kim Altered AKAP12 expression in portal fibroblasts and liver sinusoids mediates transition from hepatic fibrogenesis to fibrosis resolution Exp. Mol. Med., 2018;50(4):48. 2018 [PMID: 29700280] (IHC,, Human) IHC Human
CA Lyon, H Williams, R Bianco, SJ Simmonds, BA Brown, KS Wadey, FCT Smith, JL Johnson, SJ George Aneurysm Severity is Increased by Combined Mmp-7 Deletion and N-cadherin Mimetic (EC4-Fc) Over-Expression Sci Rep, 2017;7(1):17342. 2017 [PMID: 29229950] (ICC,, Mouse) ICC Mouse
J Kloepper, L Riedemann, Z Amoozgar, G Seano, K Susek, V Yu, N Dalvie, RL Amelung, M Datta, JW Song, V Askoxylaki, JW Taylor, C Lu-Emerson, A Batista, ND Kirkpatric, K Jung, M Snuderl, A Muzikansky, KG Stubenrauc, O Krieter, H Wakimoto, L Xu, LL Munn Ang-2/VEGF bispecific antibody reprograms macrophages and resident microglia to anti-tumor phenotype and prolongs glioblastoma survival Proc Natl Acad Sci USA, 2016;113(16):4476-81. 2016 [PMID: 27044098] (IHC - Paraffin embedded,, Mouse) IHC - Paraffin embedded Mouse
TE Peterson, ND Kirkpatric, Y Huang, CT Farrar, KA Marijt, J Kloepper, M Datta, Z Amoozgar, G Seano, K Jung, WS Kamoun, T Vardam, M Snuderl, J Goveia, S Chatterjee, A Batista, A Muzikansky, CC Leow, L Xu, TT Batchelor, DG Duda, D Fukumura, RK Jain Dual inhibition of Ang-2 and VEGF receptors normalizes tumor vasculature and prolongs survival in glioblastoma by altering macrophages Proc Natl Acad Sci USA, 2016;113(16):4470-5. 2016 [PMID: 27044097] (IHC - Paraffin embedded,, Mouse) IHC - Paraffin embedded Mouse
Liu Q, Yang R, Huang X, Zhang H, He L, Zhang L, Tian X, Nie Y, Hu S, Yan Y, Zhang L, Qiao Z, Wang Q, Lui K, Zhou B Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes. Cell Res, 0;26(1):119-30. 0 [PMID: 26634606] (IHC-Fr,, Mouse) IHC-Fr Mouse
Riordan D, Varma S, West R, Brown P Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling. PLoS ONE, 2015;10(7):e0128975. 2015 [PMID: 26176839] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Maury Y, Come J, Piskorowski R, Salah-Mohellibi N, Chevaleyre V, Peschanski M, Martinat C, Nedelec S Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes. Nat Biotechnol, 2015;33(1):89-96. 2015 [PMID: 25383599] (IHC OCT-embedded,, Human) IHC OCT-embedded Human
Durruthy-Durruthy, Jens, Briggs, Sharon F, Awe, Jason, Ramathal, Cyril Y, Karumbayaram, Saravana, Lee, Patrick, Heidmann, Julia D, Clark, Amander, Karakikes, Ioannis, Loh, Kyle M, Wu, Joseph C, Hoffman, Andrew R, Byrne, James, Reijo Pera, Renee A, Sebastiano, Vittorio Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions. PLoS ONE, 2014;9(4):e94231. 2014 [PMID: 24718618] (ICC,, Human) ICC Human
Brodehl A, Dieding M, Klauke B, Dec E, Madaan S, Huang T, Gargus J, Fatima A, Saric T, Cakar H, Walhorn V, Tonsing K, Skrzipczyk T, Cebulla R, Gerdes D, Schulz U, Gummert J, Svendsen J, Olesen M, Anselmetti D, Christensen A, Kimonis V, Milting H The novel desmin mutant p.A120D impairs filament formation, prevents intercalated disk localization, and causes sudden cardiac death. Circ Cardiovasc Genet, 2013;6(6):615-23. 2013 [PMID: 24200904] (IHC Fresh,, Human) IHC Fresh Human
Show All 11 Publications.

Reviews for Desmin Antibody (AF3844) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Cat.

Reviews using AF3844:
Filter by Applications
WB
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
Cat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Desmin AF3844
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reviewed by:
Wan-Ching Cheng
WB Cat 10/27/2018
View

Summary

ApplicationWestern Blot
Sample TestedHeart tissue
SpeciesCat
LotYIL0618061

Comments

Comments1:1000 in 1%milk/TBST overnight at 4C; washed 10 min for 3 times and incubate with mouse-anti-goat-HRP monoclonal antibody for 1 hour; washed for another 5 min 3 times. Developed with ECL.
 
reviewed by:
Anonymous
IHC-P Human 01/08/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 24200904
SpeciesHuman

FAQs for Desmin Antibody (AF3844) (0)

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Secondary Antibodies

 

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Vimentin is a fundamental 10 nm type III intermediate filament (IF) protein found in many mesenchymal and epithelia tissues, tissue culture cells, and developing neuronal and astrocytic precursor cells of the central nervous system. It frequently co-p...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Wan-Ching Cheng
10/27/2018
Application: WB
Species: Cat

 
Anonymous
01/08/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol DES
Uniprot