NCAM-1/CD56 Antibody [Unconjugated]

Images

 
Western blot shows lysates of human brain (cerebellum and motor cortex) tissue and mouse brain (cerebellum) tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse NCAM‑1/CD56 Antigen ...read more
Simple Western lane view shows lysates of human and mouse brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for NCAM‑1/CD56 at approximately 143 kDa (as indicated) using 5 µg/mL for ...read more
NCAM‑1/CD56 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neural progenitor cells using Goat Anti-Human/Mouse NCAM‑1/CD56 Antigen Affinity-purified Polyclonal Antibody (Catalog ...read more
SH-SY5Y human neuroblastoma cells were cultured overnight in the presence of 1 mM Retinoic Acid (Catalog # 0695/50) prior to immersion fixation. Neural Cell Adhesion Molecule 1 (NCAM-1)/CD56 was detected using a Goat ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, CyTOF-ready, ICC/IF, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

NCAM-1/CD56 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human NCAM1/CD56
Leu20-Pro603
Accession # NP_001070150
Specificity
Detects human NCAM-1/CD56 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) ALCAM, rhBCAM and rhEpCAM is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NCAM1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Knockout Validated 0.25 ug/mL
  • Simple Western 5-25 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF2408 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NCAM-1/CD56 Antibody [Unconjugated]

  • CD56 / NCAM-1
  • CD56 antigen
  • CD56
  • MSK39
  • NCAM1
  • N-CAM-1
  • NCAM-1
  • NCAMantigen recognized by monoclonal 5.1H11
  • neural cell adhesion molecule 1
  • neural cell adhesion molecule, NCAM

Background

Neural cell adhesion molecule 1 (NCAM-1) is a multifunctional member of the Ig superfamily. It belongs to a family of membrane-bound glycoproteins that are involved in Ca++ independent cell matrix and homophilic or heterophilic cell-cell interactions. NCAM-1 specifically binds to heparan sulfate proteoglycans (1), the extracellular matrix protein agrin (2), and several chondroitin sulfate proteoglycans that include neurocan and phosphocan (3). There are three main forms of human NCAM-1 that arise by alternate splicing. These are designated NCAM-120/NCAM-1 (761 amino acids [aa]), NCAM-140 (848 aa), and NCAM-180 (1120 aa). NCAM-120 is GPI-linked, while NCAM-140 and NCAM-180 are type I transmembrane glycoproteins (4‑6). Additional alternate splicing adds considerable diversity to all three forms, and extracellular proteolytic processing is possible for NCAM-180 (7‑8). NCAM-1 is synthesized as a 761 aa preproprecursor that contains a 19 aa signal sequence, a 722 aa GPI-linked mature region, and a 20 aa C-terminal prosegment (4). The molecule contains five C-2 type Ig-like domains and two fibronectin type-III domains. Human to mouse, NCAM-1 is 93% aa identical. NCAM-1 appears to be highly sialylated. The polysialyation of NCAM-1 reduces its adhesive property and increases its neurite outgrowth promoting features (9). NCAM-1 in the adult brain shows a decline of sialylation relative to earlier developmental periods. In regions that retain a high degree of neuronal plasticity, however, the adult brain continues to express polysialylation-NCAM-1, suggesting sialylation of NCAM-1 is involved in regenerative processes and synaptic plasticity (10‑13).

  1. Burg, M.A. et al. (1995) J. Neurosci. Res. 41:49.
  2. Storms, S.D. and U. Rutishauser (1998) J Biol. Chem. 273:27124.
  3. Margolis, R.K. et al. (1996) Perspect. Dev. Neurobiol. 3:273.
  4. Dickson, G. et al. (1987) Cell 50:1119.
  5. Lanier, L.L. et al. (1991) J. Immunol. 146:4421.
  6. Hemperly, J.J. et al. (1990) J. Mol. Neurosci. 2:71.
  7. Rutishauser, U.and C. Goridis (1986) Trends Genet. 2:72.
  8. Vawter, M.P. et al. (2001) Exp. Neurol. 172:29.
  9. Rutihauser, U. (1990) Adv. Exp. Med. Biol. 265:179.
  10. Becker, C.G. et al. (1996) J. Neurosci. Res. 45:143.
  11. Doherty, P. et al. (1995) J. Neurobiol. 26:437.
  12. Eckardt, M. et al. (2000) J. Neurosci. 20:5234.
  13. Muller, D. et al. (1996) Neuron 17:413.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NCAM-1/CD56 Antibody (AF2408)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-P, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(3)
IHC
(3)
IHC-P
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(6)
Mouse
(4)
All Species
Showing Publications 1 - 10 of 10.
Publications using AF2408 Applications Species
HW Cheng, U Mörbe, M Lütge, C Engetschwi, L Onder, M Novkovic, C Gil-Cruz, C Perez-Shib, T Hehlgans, E Scandella, B Ludewig Intestinal fibroblastic reticular cell niches control innate lymphoid cell homeostasis and function Nature Communications, 2022;13(1):2027. 2022 [PMID: 35440118] (IHC, Mouse) IHC Mouse
C Merckx, G Cosemans, J Zschüntzsc, R Raedt, J Schmidt, B De Paepe, JL De Bleecke Description of Osmolyte Pathways in Maturing Mdx Mice Reveals Altered Levels of Taurine and Sodium/Myo-Inositol Co-Transporters International Journal of Molecular Sciences, 2022;23(6):. 2022 [PMID: 35328671] (ICC, Mouse) ICC Mouse
E Hassan, R Kojima, F Ozawa, H Yoshihara, S Goto, T Kitaori, H Inagaki, Y Kato, M Sugiura-Og Abnormal ciliogenesis in decidual stromal cells in recurrent miscarriage Journal of reproductive immunology, 2022;150(0):103486. 2022 [PMID: 35085989] (IHC, Human) IHC Human
DA Clark, A Reihani, JL Arredondo, K Ask, WG Foster CD200S-positive granulated lymphoid cells in endometrium appear to be CD56-positive uterine NK cells Journal of reproductive immunology, 2022;150(0):103477. 2022 [PMID: 35051828] (IHC, Human) IHC Human
W Kim, H Watanabe, S Lomoio, G Tesco Spatiotemporal processing of neural cell adhesion molecules 1 and 2 by BACE1 in vivo The Journal of Biological Chemistry, 2021;0(0):100372. 2021 [PMID: 33548223] (Western Blot, Mouse) Western Blot Mouse
A Yilmaz, C Braverman-, A Bialer-Tsy, M Peretz, N Benvenisty Mapping Gene Circuits Essential for Germ Layer Differentiation via Loss-of-Function Screens in Haploid Human Embryonic Stem Cells Cell Stem Cell, 2020;0(0):. 2020 [PMID: 32735778] (Flow Cytometry, Human) Flow Cytometry Human
E Slot, A de Klein, RJ Rottier Generation of three iPSC lines from two patients with heterozygous FOXF1 mutations associated to Alveolar Capillary Dysplasia with Misalignment of the Pulmonary Veins Stem Cell Res, 2020;44(0):101745. 2020 [PMID: 32169823] (ICC, Human) ICC Human
YP Xu, L Lv, Y Liu, MD Smith, WC Li, XM Tan, M Cheng, Z Li, M Bovino, J Aubé, Y Xiong Tumor suppressor TET2 promotes cancer immunity and immunotherapy efficacy J. Clin. Invest., 2019;130(0):. 2019 [PMID: 31310587] (IHC-P, Mouse) IHC-P Mouse
Y Avior, E Lezmi, D Yanuka, N Benvenisty Modeling Developmental and Tumorigenic Aspects of Trilateral Retinoblastoma via Human Embryonic Stem Cells Stem Cell Reports, 2017;0(0):. 2017 [PMID: 28392220] (ICC, Human) ICC Human
Pistritto G, Papaleo V, Sanchez P, Ceci C, Barbaccia ML Divergent modulation of neuronal differentiation by caspase-2 and -9. PLoS ONE, 2012;7(5):e36002. 2012 [PMID: 22629307] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol NCAM1
Uniprot