beta Amyloid Antibody (MOAB-2)

Immunohistochemistry: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of beta Amyloid on normal mouse brain (left) and 5xFAD mouse brain (right) using DAB with hematoxylin counterstain. The MOAB-2 antibody was used at 1:20 on normal mouse brain and at 1:400 on 5xFAD mouse brain.

Immunohistochemistry-Paraffin: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of a formalin fixed paraffin embedded tissue section of human brain (Alzheimers disease, hippocampus) using 1:200 dilution of anti-beta Amyloid antibody (clone MOAB-2). The staining was developed with HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This beta Amyloid antibody specifically stained the cells with Abeta 42/ Abeta aggregates while the normal cells were negative for abeta peptide.

Western Blot: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Western blot analysis in cell lysates from HEK-APP SWE/BACE1 cells probed with an antibody against the C-terminus of APP (Lanes 1 and 2) and beta Amyloid (MOAB-2, Lanes 3 and 4). Beta Amyloid (MOAB-2) does not detect APP (from PMID: 22423893).

Western Blot: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Analysis of beta Amyloid (MOAB-2) antibody in 1) 100 pmole beta Amyloid 42, 2) 5xFAD mouse brain homogenate Repetition 1 and 3) 5xFAD mouse brain homogenate Repetition 2.

Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Immunofluorescent detection of beta Amyloid with MOAB-2 in the subiculim of 1-, 2- and 4- month old 5xFAD mice. Scale bar 20 um (from PMID: 22423893).

Immunohistochemistry-Paraffin: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of a formalin fixed paraffin embedded tissue section of human brain (Alzheimers disease, hippocampus) using 1:40 dilution of anti-beta Amyloid antibody (clone MOAB-2). The staining was developed with HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This beta Amyloid antibody specifically stained the cells with Abeta 42/ Abeta aggregates while the normal cells were negative for abeta peptide.

• Reactivity: Hu, Mu, Rt, Mk
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, IHC-FrFl
• Clone: MOAB-2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

beta Amyloid Antibody (MOAB-2) Summary

• Immunogen: Recombinant human beta Amyloid 42
• Epitope: Residues 1-4 of beta Amyloid
• Specificity: Unaggregated, oligomeric and fibrillar forms of beta Amyloid 42 and unaggregated beta Amyloid 40. Does not detect APP.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: APP
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:5000
  • ELISA 1:100-1:1000
  • Immunocytochemistry/Immunofluorescence 1:200-1:500
  • Immunohistochemistry 1:40-1:400
  • Immunohistochemistry-Paraffin 1:40-1:400
  • Immunoprecipitation 1:200-1:1000
  • Immunohistochemistry Free-Floating
• Application Notes: This beta Amyloid Antibody (MOAB-2) is useful for Immunocytochemistry/Immunofluorescence, ELISA, Immunoprecipitation, Western Blot, and Immunohistochemistry on paraffin-embedded sections. In Western blot, a band can be seen at ~4 kDa, representing the beta Amyloid monomer. Larger bands may also be seen representing the unaggregated, oligomeric, and fibrillar forms of beta Amyloid. For higher beta Amyloid yield in WB, please follow the extraction protocol described in Youmans et al, J Neurosci Methods. 2011 March 15; 196(1): 51-59 (PMID: 21219931). Use in Immunohistochemistry-Free floating reported in scientific literature (PMID 25747037)

Reactivity Notes

Human, mouse and rat. Monkey reactivity reported in scientific literature (PMID: 29241829).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Notes

5xFAD mice = transgenic mice expressing 5 familial Alzheimer's disease mutation. See Oakley H et al, J Neurosci. 2006 Oct 4;26(40):10129-40 (PMID: 17021169) for more information on 5xFAD mice.

Alternate Names for beta Amyloid Antibody (MOAB-2)

• Abeta
• Amyloid beta
• beta-Amyloid Peptide

Background

Beta Amyloid, also known as Abeta, is a peptide that varies in length from 39 to 43 amino acids and is processed from the Amyloid precursor protein (APP). Beta Amyloid is known as the main component of amyloid plaques, which are deposits found in the brains of patients with Alzheimer's disease. The form(s) of beta Amyloid associated with the pathology characteristic of Alzheimer's disease still remains unclear. Currently, controversy exists as to the existence of intraneuronal beta Amyloid. Research indicates that intraneuronal beta Amyloid accumulation may be an important proximal neurotoxic event in the pathogenesis of Alzheimer's disease. Many commercially available beta Amyloid antibodies cross react with APP or APP C-terminal fragments. This MOAB-2 antibody is specific for beta Amyloid but not APP. MOAB-2 is a pan-specific beta Amyloid antibody that detects unaggregated, oligomeric and fibrillar forms of beta Amyloid. This is important because beta Amyloid is known to aggregate and form a variety of potentially toxic conformations. MOAB-2 displays strong extracellular beta Amyloid immunoreactivity, and so detects classic plaque pathology. MOAB-2 is selective for Abeta 42 over Abeta 40. This is also an advantage as the 42 amino acid form of beta Amyloid is likely the toxic form. MOAB-2 demonstrates strong intraneuronal immunoreactivity in both human and beta Amyloid-Tg mouse tissue and has the potential to aid future research into the functional importance of intraneuronal beta Amyloid.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta Amyloid Antibody (NBP2-13075)(12)

Publications using NBP2-13075

• Stuart KE, King AE, Fernandez-Martos CM et al. Mid-life environmental enrichment increases synaptic density in CA1 in a mouse model of Ab-associated pathology and positively influences synaptic and cognitive health in healthy ageing. J. Comp. Neurol. Dec 17 2016 [PMID: 27987205]
• Yu YW, Hsieh TH, Chen KY et al. Glucose-dependent insulinotropic polypeptide ameliorates mild traumatic brain injury-induced cognitive and sensorimotor deficits and neuroinflammation in rats J. Neurotrauma Mar 14 2016 [PMID: 26972789] (WB, Rat)
• Collins JM, King AE, Woodhouse A et al. The effect of focal brain injury on beta-amyloid plaque deposition, inflammation and synapses in the APP/PS1 mouse model of Alzheimer's disease Exp. Neurol. 2015 Mar 04 [PMID: 25747037] (IHC-FrFl, Mouse)
  
  Details:
  beta Amyloid antibody (clone MOAB-2) used for IHC immunostaining on free floating brain sections from experiments involving focal brain injury in 3 or 9 months old male APPSWE/PSENdE9 (APP/PS1) transgenic mice (a murine model of Alzheimer's disease, Jankowsky et al. 2001 Biomol Eng. 17: 157-165 ) and C57BL/6 wild-type mice. The assay involved 4% paraformaldehyde-PBS fixation, 8 minutes of antigen retrieval with 88% formic acid at RT, permeablization 3X10 minutes each using 0.25% Triton-X-100-PBS, 15 minutes blocking with Dako serum-free protein block, primary used at 1:2000 dilution with ON 4C incubation, detection using Alexa-fluorophore conjugated secondary antibodies (see full text for detailed protocol and staining images).
• Kim JA, Yun HM, Jin P et al. Inhibitory effect of a 2,4-bis(4-hydroxyphenyl)but-2-enal diacetate on neuro-inflammatory reactions via inhibition of STAT1 and STAT3 activation in cultured astrocytes and microglial BV-2 cells. Neuropharmacology 2013 Jul 23 [PMID: 23891616] (ICC/IF, Rat)
• Youmans KL, Tai LM, Nwabuisi-Heath E et al. APOE4-specific Changes in A? Accumulation in a New Transgenic Mouse Model of Alzheimer Disease J Biol Chem 2012 Dec 7 [PMID: 23060451] (ELISA, IHC, Human)
• Tai, LM, Youmans, K et al. Intraneuronal Abeta detection in 5xFAD mice by a new Abeta-specific antibody Molecular Neurodegeneration 2012 [PMID: 22423893] (ICC/IF, WB, IP, DB, Mouse)
• Lee CL, Lin PY, Hsu YW, Pan TM. Monascus-fermented monascin and ankaflavin improve the memory and learning ability in amyloid beta-protein intracerebroventricular-infused rat via the suppression of Alzheimer's disease risk factors. Journal of Functional Foods. 2015 Oct 01 (IHC-Fr, Rat)
  
  Details:
  beta Amyloid antibody used for immunohistochemical staining of rat brain frozen tissue at a dilution of 1:100 (Fig. 7B).
• Kim DH, Lim H, Lee D et al. Thrombospondin-1 secreted by human umbilical cord blood-derived mesenchymal stem cells rescues neurons from synaptic dysfunction in Alzheimer's disease model Sci Rep 2018 Jan 10 [PMID: 29321508] (WB)
• Paspalas CD, Carlyle BC, Leslie S et al. The aged rhesus macaque manifests Braak stage III/IV Alzheimer's-like pathology. Alzheimers Dement 2017 Dec 11 [PMID: 29241829] (ICC/IF, Monkey)
• Lee DY, Hwang CJ, Choi JY et al. KRICT-9 inhibits neuroinflammation, amyloidogenesis and memory loss in Alzheimer's disease models. Oncotarget. 2017 Sep 15 [PMID: 28978145] (ICC/IF, Mouse)
• Stuart KE, King AE, Fernandez-Martos CM et al. Environmental novelty exacerbates stress hormones and AB pathology in an Alzheimer's model. Sci Rep. 2017 Jun 05 [PMID: 28584278] (ICC/IF, Mouse)
• Balczon R, Morrow KA, Zhou C et al. Pseudomonas aeruginosa infection liberates transmissible, cytotoxic prion amyloids. FASEB J. Mar 17 2017 [PMID: 28314768]

FAQs for beta Amyloid Antibody (NBP2-13075). (Showing 1 - 1 of 1 FAQs).

1. Does your antibody beta Amyloid Antibody (MOAB-2) # NBP2-13075 work on frozen sections for histology?
   • This antibody has never been tested on frozen sections. It has been validated in ICC, IP and IHC on paraffin-embedded tissues, so it may work on frozen sections. While we cannot guarantee that it will work on frozen sections, if you would like to test NBP2-13075 in an untested application and share your results with us, I can recommend our Innovator's Reward Program. Our Innovator's Reward program was created to allow researchers the opportunity to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply submit an online review detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Reviews may also be submitted by emailing innovators@novusbio.com.

Bioinformatics

• Gene Symbol: APP
• Entrez:
  • 351(Human)
  • 11820(Mouse)
• Uniprot:
  • P05067()