beta Amyloid Antibody (MOAB-2)

Images

 
Immunohistochemistry: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of beta Amyloid on normal mouse brain (left) and 5xFAD mouse brain (right) using DAB with hematoxylin counterstain. The MOAB-2 antibody ...read more
Immunocytochemistry/ Immunofluorescence: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Immunofluorescent detection of beta Amyloid with MOAB-2 in the subiculim of 1-, 2- and 4- month old 5xFAD mice. Scale bar 20 um ...read more
Western Blot: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Analysis of beta Amyloid (MOAB-2) antibody in (1) 100 pmole beta Amyloid 42, (2) 5xFAD mouse brain homogenate Repetition 1 and (3) 5xFAD mouse brain homogenate ...read more
Western Blot: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Western blot analysis in cell lysates from HEK-APP SWE/BACE1 cells probed with an antibody against the C-terminus of APP (Lanes 1 and 2) and beta Amyloid ...read more
Immunohistochemistry-Paraffin: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of a formalin fixed paraffin embedded tissue section of human brain (Alzheimers disease, hippocampus) using 1:200 dilution of ...read more
Immunohistochemistry-Frozen: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Mouse brain (cerebral cortex). Red: MOAB-2 antibody staining, Blue: DAPI. Zeiss LSM800, 40x. Image from verified customer review.
Immunohistochemistry-Paraffin: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of a formalin fixed paraffin embedded tissue section of human brain (Alzheimers disease, hippocampus) using 1:40 dilution of ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, DB, ELISA, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
Clone
MOAB-2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

beta Amyloid Antibody (MOAB-2) Summary

Immunogen
This beta Amyloid antibody was developed against recombinant human beta Amyloid 42.
Epitope
Residues 1-4 of beta Amyloid
Specificity
Beta Amyloid antibody (MOAB-2) recognizes unaggregated, oligomeric and fibrillar forms of beta Amyloid 42 and unaggregated beta Amyloid 40. Does not detect APP.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
APP
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Dot Blot
  • ELISA 1:100-1:1000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:200-1:500
  • Immunohistochemistry 1:40-1:400
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:40-1:400
  • Immunoprecipitation 1:200-1:1000
  • Immunohistochemistry Free-Floating
Application Notes
This beta Amyloid Antibody (MOAB-2) is useful for Immunocytochemistry/Immunofluorescence, ELISA, Immunoprecipitation, Western Blot, and Immunohistochemistry on paraffin-embedded sections. In Western blot, a band can be seen at ~4 kDa, representing the beta Amyloid monomer. Larger bands may also be seen representing the unaggregated, oligomeric, and fibrillar forms of beta Amyloid. For higher beta Amyloid yield in WB, please follow the extraction protocol described in Youmans et al, J Neurosci Methods. 2011 March 15; 196(1): 51-59 (PMID: 21219931). Use in Immunohistochemistry-Free floating reported in scientific literature (PMID 25747037). Use in Dot blot reported in scientific literature (PMID: 22423893). Use in immunoblotting reported in scientific literature (PMID: 28314768).
Theoretical MW
5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP2-13075 in the following applications:

Publications
Read Publications using
NBP2-13075 in the following applications:

Reactivity Notes

Human, mouse and rat. Monkey reactivity reported in scientific literature (PMID: 29241829).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Notes

5xFAD mice = transgenic mice expressing 5 familial Alzheimer's disease mutation. See Oakley H et al, J Neurosci. 2006 Oct 4;26(40):10129-40 (PMID: 17021169) for more information on 5xFAD mice.

Alternate Names for beta Amyloid Antibody (MOAB-2)

  • Abeta
  • Amyloid beta
  • beta-Amyloid Peptide

Background

Beta amyloid, also known as Abeta, is a peptide that varies in length from 39 to 43 amino acids and is processed from the transmembrane protein amyloid precursor protein (APP) by beta-secretase (BACE-1) and gamma-secretase. Beta amyloid [1-40], beta amyloid [1-42], and beta amyloid [1-43] peptides result from cleavage of APP after residues 40, 42, and 43, respectively. The aggregation of beta amyloid monomers produces insoluble oligomers and protofibrils. The longer "stickier" forms of beta amyloid, particularly ABeta42, are known as the main component of amyloid plaques, which are deposits (senile plaques or neuritic plaques) found in the brains of patients with Alzheimer's disease (AD) (1,2). Research indicates that intraneuronal beta amyloid accumulation may be an important proximal neurotoxic event in the pathogenesis of Alzheimer's disease. Abeta deposits have also been reported in the hearts of AD patients (3).

Pyroglutamate amyloid beta peptides (pGlu-Abeta) are N-terminal truncations in which the N-terminal glutamate is cyclized to pyroglutamate resulting in pGlu-Abeta (3-40/42 and 11-40/42) (4). This pyrE modification may have a greater propensity to aggregate under physiological conditions and has been implicated as the molecular species responsible for seeding larger oligomers of amyloid beta.

References

1. Chen GF, Xu TH, Yan Y, Zhou YR, Jiang Y, Melcher K, Xu HE. (2017) Amyloid beta: structure, biology and structure-based therapeutic development. Acta Pharmacol Sin. 38(9):1205-1235. PMID: 28713158

2. De-Paula VJ1, Radanovic M, Diniz BS, Forlenza OV. (2012) Alzheimer's disease. Subcell Biochem. 65:329-52. PMID: 23225010

3. Schaich CL, Maurer MS, Nadkarni NK. (2019) Amyloidosis of the Brain and Heart: Two Sides of the Same Coin? JACC Heart Fail. 7(2):129-131. PMID: 30704604

4. He W, Barrow CJ. (1999) The A beta 3-pyroglutamyl and 11-pyroglutamyl peptides found in senile plaque have greater beta-sheet forming and aggregation propensities in vitro than full-length A beta. Biochemistry. 38(33):10871-7. PMID: 10451383

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta Amyloid Antibody (NBP2-13075)(24)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Monkey.

We have publications tested in 9 applications: DB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-FrFl, IHC-P, IP, WB.


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DB
(1)
ELISA
(1)
ICC/IF
(6)
IHC
(3)
IHC-Fr
(1)
IHC-FrFl
(2)
IHC-P
(1)
IP
(1)
WB
(6)
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Human
(5)
Mouse
(10)
Rat
(3)
Monkey
(1)
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Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NBP2-13075 Applications Species
El Hajjar J, Chatoo W, Hanna R, Nkanza P. Heterochromatic genome instability and neurodegeneration sharing similarities with Alzheimer's disease in old Bmi1 +/- mice Scientific reports Jul 11 1905 (WB, Human) WB Human
He Y, Ruganzu JB, Lin C et al. Tanshinone IIA ameliorates cognitive deficits by inhibiting endoplasmic reticulum stress-induced apoptosis in APP/PS1 transgenic mice Neurochem. Int. Nov 25 2019 [PMID: 31778727] (IHC, Mouse) IHC Mouse
Lee CL, Lin PY, Hsu YW, Pan TM. Monascus-fermented monascin and ankaflavin improve the memory and learning ability in amyloid beta-protein intracerebroventricular-infused rat via the suppression of Alzheimer's disease risk factors. Journal of Functional Foods. 2015 Oct 01 (IHC-Fr, Rat)

Details:
beta Amyloid antibody used for immunohistochemical staining of rat brain frozen tissue at a dilution of 1:100 (Fig. 7B).
IHC-Fr Rat
Joseph E, Villalobos-Acosta DMA, Torres-Ramos MA et al. Neuroprotective Effects of Apocynin and Galantamine During the Chronic Administration of Scopolamine in an Alzheimer's Disease Model J. Mol. Neurosci. Nov 25 2019 [PMID: 31768942]
Prokop S, Miller KR, Labra SR et al. Impact of TREM2 risk variants on brain region-specific immune activation and plaque microenvironment in Alzheimer's disease patient brain samples Acta Neuropathol. Jul 26 2019 [PMID: 31350575] (IHC-P, Human) IHC-P Human
Balczon R, Pittet JF, Wagener BM et al. Infection-induced endothelial amyloids impair memory FASEB J. Jun 18 2019 [PMID: 31211919]
Tian C, Liu G, Gao L Erythrocytic alpha -Synuclein as a potential biomarker for Parkinson's disease Transl Neurodegener May 15 2019 [PMID: 31123587] (Human) Human
El Hajjar J, Chatoo W, Hanna R et al. Heterochromatic genome instability and neurodegeneration sharing similarities with Alzheimer's disease in old Bmi1+/- mice. Sci Rep 2019 Jan 24 [PMID: 30679733] (WB, Mouse)

Details:
This beta Amyloid antibody was used in Western Blot experiments evaluating the use of aging Bmi1+/- as a model to study novel pathogenic AD-related mechanisms.
WB Mouse
Cristiano C, Volpicelli F, Lippiello P et al. Neutralization of interleukin-17 rescues amyloid-b-induced neuroinflammation and memory impairment. Br. J. Pharmacol. Jan 23 2019 [PMID: 30673121] (WB, Mouse) WB Mouse
Collins JM, King AE, Woodhouse A et al. Age moderates the effects of traumatic brain injury on beta-amyloid plaque load in APP/PS1 mice. J. Neurotrauma Jan 9 2019 [PMID: 30623730] (IHC-FrFl, Mouse) IHC-FrFl Mouse
Show All 24 Publications.

Reviews for beta Amyloid Antibody (NBP2-13075) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, 5xFAD mouse.

Reviews using NBP2-13075:
Filter by Applications
WB
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
5xFAD mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot beta Amyloid NBP2-13075
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reviewed by:
Anonymous
WB Mouse 12/11/2019
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Summary

ApplicationWestern Blot
Sample Testedneonatal cardiac myocytes
SpeciesMouse
LotF-3
Immunohistochemistry-Frozen beta Amyloid NBP2-13075
Enlarge
reviewed by:
Lei Yao
IHC-Fr 5xFAD mouse 09/22/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedBrain (cerebral cortex)
Species5xFAD mouse
LotE-1

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta Amyloid Antibody (NBP2-13075). (Showing 1 - 2 of 2 FAQs).

  1. Does your antibody beta Amyloid Antibody (MOAB-2) # NBP2-13075 work on frozen sections for histology?
    • This antibody has never been tested on frozen sections. It has been validated in ICC, IP and IHC on paraffin-embedded tissues, so it may work on frozen sections. While we cannot guarantee that it will work on frozen sections, if you would like to test NBP2-13075 in an untested application and share your results with us, I can recommend our Innovator's Reward Program. Our Innovator's Reward program was created to allow researchers the opportunity to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply submit an online review detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Reviews may also be submitted by emailing innovators@novusbio.com.
  2. Please provide the epitope region information for NBP2-13075 Beta Amyloid Antibody.
    • The epitope is a.a. 672 - 675 and please use https://www.uniprot.org/uniprot/P05067#sequences to check all important information.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-13075AF350
Alexa Fluor 405 NBP2-13075AF405
Alexa Fluor 488 NBP2-13075AF488
Alexa Fluor 532 NBP2-13075AF532
Alexa Fluor 594 NBP2-13075AF594
Alexa Fluor 647 NBP2-13075AF647
Alexa Fluor 700 NBP2-13075AF700
Alexa Fluor 750 NBP2-13075AF750
Biotin NBP2-13075B
DyLight 350 NBP2-13075UV
DyLight 405 NBP2-13075V
DyLight 488 NBP2-13075G
DyLight 550 NBP2-13075R
DyLight 594 NBP2-13075DL594
DyLight 650 NBP2-13075C
DyLight 680 NBP2-13075FR
DyLight 755 NBP2-13075IR
FITC NBP2-13075F
HRP NBP2-13075H
Janelia Fluor 549 NBP2-13075JF549
Janelia Fluor 646 NBP2-13075JF646

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Blogs on beta Amyloid.

Mechanisms of Neurodegeneration: Neuroinflammation and microglial activation
By Michalina Hanzel, PhDIn this final instalment of our three blog-posts series on major cellular mechanisms responsible for neurodegenerative disorders, we will explore the processes of neuroinflammation and microgl...  Read full blog post.

Immunity’s flipside: Microglia promote Alzheimer’s pathology during inflammation
By Jamshed Arslan Pharm.D. Microglia are brain's macrophages. In Alzheimer's disease (AD), microglia clear up protein aggregates called amyloid beta plaques. The connection between immune activation and AD is unclea...  Read full blog post.

Beta Amyloid (MOAB2) and the link between traumatic brain injury and Alzheimer’s disease
An epidemiological association between traumatic brain injury (TBI) and Alzheimer's disease (AD) has long been established.  Interestingly, an increase in beta amyloid  (one hallmark of AD) directly following TBI has been observed.  In fact, it h...  Read full blog post.

Analysis of Total & pSer724 IRE1 alpha, the Sensor of ER Stress
Inositol-requiring protein 1/IRE1 alpha (also called Endoplasmic Reticulum to Nucleus Signaling 1/ERN1; predicted mol wt 110 kDa) is a serine-threonine protein kinase/endoribonuclease which plays a highly critical role in unfolded protein response/U...  Read full blog post.

Beta Amyloid Neurotoxicity and Alzheimer's Disease
A major histopathological hallmark of Alzheimer's disease (AD) is the presence of amyloid deposits in the parenchyma of the amygdala, hippocampus, and neocortex. The principal component of amyloid is beta amyloid (AB). The pathologic accumulation of A...  Read full blog post.

Using Amyloid beta peptides in Alzheimer's Disease Immunization
Amyloid beta (AB) peptide has a central role in the neurodegeneration of Alzheimer's disease (AD). Immunization of AD transgenic mice with AB-42 peptide reduces both the spatial memory impairments and AD-like neuropathologic changes.Therapeutic im...  Read full blog post.

Synaptophysin and Dementing Disorders
 Synaptophysin (a presynaptic vesicle protein) is an integral membrane glycoprotein originally isolated from presynaptic vesicles of bovine neurons. Synaptophysin is found in all nerve terminals and synaptophysin measurements have been used to quantif...  Read full blog post.

Amyloid beta and Methionine Sulfoxide Related to Abeta 42 Antibody and Abeta 40 Antibody
By Eric NeeleyAlzheimer's disease is a devastating neurodegenerative illness characterized by the formation of plaques, tangles, and eventually synaptic loss. Amyloid beta (A beta ) is the processed form of the Amyloid precursor protein (APP), and whose...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Anonymous
12/11/2019
Application: WB
Species: Mouse

Lei Yao
09/22/2018
Application: IHC-Fr
Species: 5xFAD mouse

Bioinformatics

Gene Symbol APP
Entrez
Uniprot
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