beta Amyloid Antibody (MOAB-2)

Images

 
Immunohistochemistry: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of beta Amyloid on normal mouse brain (left) and 5xFAD mouse brain (right) using DAB with hematoxylin counterstain. The MOAB-2 antibody ...read more
Immunohistochemistry-Paraffin: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of a formalin fixed paraffin embedded tissue section of human brain (Alzheimers disease, hippocampus) using 1:200 dilution of ...read more
Western Blot: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Western blot analysis in cell lysates from HEK-APP SWE/BACE1 cells probed with an antibody against the C-terminus of APP (Lanes 1 and 2) and beta Amyloid ...read more
Western Blot: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Analysis of beta Amyloid (MOAB-2) antibody in 1) 100 pmole beta Amyloid 42, 2) 5xFAD mouse brain homogenate Repetition 1 and 3) 5xFAD mouse brain homogenate ...read more
Immunocytochemistry/ Immunofluorescence: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Immunofluorescent detection of beta Amyloid with MOAB-2 in the subiculim of 1-, 2- and 4- month old 5xFAD mice. Scale bar 20 um ...read more
Immunohistochemistry-Paraffin: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of a formalin fixed paraffin embedded tissue section of human brain (Alzheimers disease, hippocampus) using 1:40 dilution of ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP, IHC-FrFl
Clone
MOAB-2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
     

Genetic Strategies

   

beta Amyloid Antibody (MOAB-2) Summary

Immunogen
Recombinant human beta Amyloid 42
Epitope
Residues 1-4 of beta Amyloid
Specificity
Unaggregated, oligomeric and fibrillar forms of beta Amyloid 42 and unaggregated beta Amyloid 40. Does not detect APP.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
APP
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • ELISA 1:100-1:1000
  • Immunocytochemistry/Immunofluorescence 1:200-1:500
  • Immunohistochemistry 1:40-1:400
  • Immunohistochemistry-Paraffin 1:40-1:400
  • Immunoprecipitation 1:200-1:1000
  • Immunohistochemistry Free-Floating
Application Notes
This beta Amyloid Antibody (MOAB-2) is useful for Immunocytochemistry/Immunofluorescence, ELISA, Immunoprecipitation, Western Blot, and Immunohistochemistry on paraffin-embedded sections. In Western blot, a band can be seen at ~4 kDa, representing the beta Amyloid monomer. Larger bands may also be seen representing the unaggregated, oligomeric, and fibrillar forms of beta Amyloid. For higher beta Amyloid yield in WB, please follow the extraction protocol described in Youmans et al, J Neurosci Methods. 2011 March 15; 196(1): 51-59 (PMID: 21219931). Use in Immunohistochemistry-Free floating reported in scientific literature (PMID 25747037)
Publications
Read Publications using
NBP2-13075 in the following applications:

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Notes

5xFAD mice = transgenic mice expressing 5 familial Alzheimer's disease mutation. See Oakley H et al, J Neurosci. 2006 Oct 4;26(40):10129-40 (PMID: 17021169) for more information on 5xFAD mice.

Alternate Names for beta Amyloid Antibody (MOAB-2)

  • Abeta
  • Amyloid beta
  • beta-Amyloid Peptide

Background

Beta Amyloid, also known as Abeta, is a peptide that varies in length from 39 to 43 amino acids and is processed from the Amyloid precursor protein (APP). Beta Amyloid is known as the main component of amyloid plaques, which are deposits found in the brains of patients with Alzheimer's disease. The form(s) of beta Amyloid associated with the pathology characteristic of Alzheimer's disease still remains unclear. Currently, controversy exists as to the existence of intraneuronal beta Amyloid. Research indicates that intraneuronal beta Amyloid accumulation may be an important proximal neurotoxic event in the pathogenesis of Alzheimer's disease. Many commercially available beta Amyloid antibodies cross react with APP or APP C-terminal fragments. This MOAB-2 antibody is specific for beta Amyloid but not APP. MOAB-2 is a pan-specific beta Amyloid antibody that detects unaggregated, oligomeric and fibrillar forms of beta Amyloid. This is important because beta Amyloid is known to aggregate and form a variety of potentially toxic conformations. MOAB-2 displays strong extracellular beta Amyloid immunoreactivity, and so detects classic plaque pathology. MOAB-2 is selective for Abeta 42 over Abeta 40. This is also an advantage as the 42 amino acid form of beta Amyloid is likely the toxic form. MOAB-2 demonstrates strong intraneuronal immunoreactivity in both human and beta Amyloid-Tg mouse tissue and has the potential to aid future research into the functional importance of intraneuronal beta Amyloid.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-13075
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, IHC-FrFl

Publications for beta Amyloid Antibody (NBP2-13075)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: DB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-FrFl, IP, WB.


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DB
(1)
ELISA
(1)
ICC/IF
(4)
IHC
(1)
IHC-Fr
(1)
IHC-FrFl
(1)
IP
(1)
WB
(2)
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Filter By Species
Human
(1)
Mouse
(4)
Rat
(3)
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Showing Publications 1 - 10 of 10.
Publications using NBP2-13075 Applications Species
Lee DY, Hwang CJ, Choi JY et al. KRICT-9 inhibits neuroinflammation, amyloidogenesis and memory loss in Alzheimer's disease models. Oncotarget. 2017 Sep 15 [PMID: 28978145] (ICC/IF, Mouse) ICC/IF Mouse
Stuart KE, King AE, Fernandez-Martos CM et al. Environmental novelty exacerbates stress hormones and AB pathology in an Alzheimer's model. Sci Rep. 2017 Jun 05 [PMID: 28584278] (ICC/IF, Mouse) ICC/IF Mouse
Balczon R, Morrow KA, Zhou C et al. Pseudomonas aeruginosa infection liberates transmissible, cytotoxic prion amyloids. FASEB J. Mar 17 2017 [PMID: 28314768]
Stuart KE, King AE, Fernandez-Martos CM et al. Mid-life environmental enrichment increases synaptic density in CA1 in a mouse model of Ab-associated pathology and positively influences synaptic and cognitive health in healthy ageing. J. Comp. Neurol. Dec 17 2016 [PMID: 27987205]
Yu YW, Hsieh TH, Chen KY et al. Glucose-dependent insulinotropic polypeptide ameliorates mild traumatic brain injury-induced cognitive and sensorimotor deficits and neuroinflammation in rats J. Neurotrauma Mar 14 2016 [PMID: 26972789] (WB, Rat) WB Rat
Collins JM, King AE, Woodhouse A et al. The effect of focal brain injury on beta-amyloid plaque deposition, inflammation and synapses in the APP/PS1 mouse model of Alzheimer's disease Exp. Neurol. 2015 Mar 04 [PMID: 25747037] (IHC-FrFl, Mouse)

Details:
beta Amyloid antibody (clone MOAB-2) used for IHC immunostaining on free floating brain sections from experiments involving focal brain injury in 3 or 9 months old male APPSWE/PSENdE9 (APP/PS1) transgenic mice (a murine model of Alzheimer's disease, Jankowsky et al. 2001 Biomol Eng. 17: 157-165 ) and C57BL/6 wild-type mice. The assay involved 4% paraformaldehyde-PBS fixation, 8 minutes of antigen retrieval with 88% formic acid at RT, permeablization 3X10 minutes each using 0.25% Triton-X-100-PBS, 15 minutes blocking with Dako serum-free protein block, primary used at 1:2000 dilution with ON 4C incubation, detection using Alexa-fluorophore conjugated secondary antibodies (see full text for detailed protocol and staining images).
IHC-FrFl Mouse
Kim JA, Yun HM, Jin P et al. Inhibitory effect of a 2,4-bis(4-hydroxyphenyl)but-2-enal diacetate on neuro-inflammatory reactions via inhibition of STAT1 and STAT3 activation in cultured astrocytes and microglial BV-2 cells. Neuropharmacology 2013 Jul 23 [PMID: 23891616] (ICC/IF, Rat) ICC/IF Rat
Youmans KL, Tai LM, Nwabuisi-Heath E et al. APOE4-specific Changes in A? Accumulation in a New Transgenic Mouse Model of Alzheimer Disease J Biol Chem 2012 Dec 7 [PMID: 23060451] (ELISA, IHC, Human) ELISA, IHC Human
Tai, LM, Youmans, K et al. Intraneuronal Abeta detection in 5xFAD mice by a new Abeta-specific antibody Molecular Neurodegeneration 2012 [PMID: 22423893] (ICC/IF, WB, IP, DB, Mouse) ICC/IF, WB, IP, DB Mouse
Lee CL, Lin PY, Hsu YW, Pan TM. Monascus-fermented monascin and ankaflavin improve the memory and learning ability in amyloid beta-protein intracerebroventricular-infused rat via the suppression of Alzheimer's disease risk factors. Journal of Functional Foods. 2015 Oct 01 (IHC-Fr, Rat)

Details:
beta Amyloid antibody used for immunohistochemical staining of rat brain frozen tissue at a dilution of 1:100 (Fig. 7B).
IHC-Fr Rat

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta Amyloid Antibody (NBP2-13075). (Showing 1 - 1 of 1 FAQs).

  1. Does your antibody beta Amyloid Antibody (MOAB-2) # NBP2-13075 work on frozen sections for histology?
    • This antibody has never been tested on frozen sections. It has been validated in ICC, IP and IHC on paraffin-embedded tissues, so it may work on frozen sections. While we cannot guarantee that it will work on frozen sections, if you would like to test NBP2-13075 in an untested application and share your results with us, I can recommend our Innovator's Reward Program. Our Innovator's Reward program was created to allow researchers the opportunity to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply submit an online review detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Reviews may also be submitted by emailing innovators@novusbio.com.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-13075AF405
Alexa Fluor 488 Labeled NBP2-13075AF488
Alexa Fluor 647 Labeled NBP2-13075AF647
Alexa Fluor 700 Labeled NBP2-13075AF700
Biotin Labeled NBP2-13075B
DyLight 405 Labeled NBP2-13075V
DyLight 488 Labeled NBP2-13075G
DyLight 550 Labeled NBP2-13075R
DyLight 650 Labeled NBP2-13075C
FITC Labeled NBP2-13075F
HRP Labeled NBP2-13075H

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Blogs on beta Amyloid.

Beta Amyloid (MOAB2) and the link between traumatic brain injury and Alzheimer’s disease
An epidemiological association between traumatic brain injury (TBI) and Alzheimer's disease (AD) has long been established.  Interestingly, an increase in beta amyloid  (one hallmark of AD) directly following TBI has been observed.  In fact, it h...  Read full blog post.

Analysis of Total & pSer724 IRE1 alpha, the Sensor of ER Stress
Inositol-requiring protein 1/IRE1 alpha (also called Endoplasmic Reticulum to Nucleus Signaling 1/ERN1; predicted mol wt 110 kDa) is a serine-threonine protein kinase/endoribonuclease which plays a highly critical role in unfolded protein response/U...  Read full blog post.

Beta Amyloid Neurotoxicity and Alzheimer's Disease
A major histopathological hallmark of Alzheimer's disease (AD) is the presence of amyloid deposits in the parenchyma of the amygdala, hippocampus, and neocortex. The principal component of amyloid is beta amyloid (AB). The pathologic accumulation of A...  Read full blog post.

Using Amyloid beta peptides in Alzheimer's Disease Immunization
Amyloid beta (AB) peptide has a central role in the neurodegeneration of Alzheimer's disease (AD). Immunization of AD transgenic mice with AB-42 peptide reduces both the spatial memory impairments and AD-like neuropathologic changes.Therapeutic im...  Read full blog post.

Synaptophysin and Dementing Disorders
 Synaptophysin (a presynaptic vesicle protein) is an integral membrane glycoprotein originally isolated from presynaptic vesicles of bovine neurons. Synaptophysin is found in all nerve terminals and synaptophysin measurements have been used to quantif...  Read full blog post.

Amyloid beta and Methionine Sulfoxide Related to Abeta 42 Antibody and Abeta 40 Antibody
By Eric NeeleyAlzheimer's disease is a devastating neurodegenerative illness characterized by the formation of plaques, tangles, and eventually synaptic loss. Amyloid beta (A beta ) is the processed form of the Amyloid precursor protein (APP), and whose...  Read full blog post.

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Bioinformatics

Gene Symbol APP
Entrez
Uniprot