beta Amyloid Antibody (MOAB-2)

Immunohistochemistry: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of beta Amyloid on normal mouse brain (left) and 5xFAD mouse brain (right) using DAB with hematoxylin counterstain. The MOAB-2 antibody was used at 1:20 on normal mouse brain and at 1:400 on 5xFAD mouse brain.

Western Blot: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Analysis of beta Amyloid (MOAB-2) antibody in (1) 100 pmole beta Amyloid 42, (2) 5xFAD mouse brain homogenate Repetition 1 and (3) 5xFAD mouse brain homogenate Repetition 2.

Western Blot: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Western blot analysis in cell lysates from HEK-APP SWE/BACE1 cells probed with an antibody against the C-terminus of APP (Lanes 1 and 2) and beta Amyloid (MOAB-2, Lanes 3 and 4). Beta Amyloid (MOAB-2) does not detect APP (from PMID: 22423893).

Immunohistochemistry-Paraffin: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of a formalin fixed paraffin embedded tissue section of human brain (Alzheimers disease, hippocampus) using 1:200 dilution of anti-beta Amyloid antibody (clone MOAB-2). The staining was developed with HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This beta Amyloid antibody specifically stained the cells with Abeta 42/ Abeta aggregates while the normal cells were negative for abeta peptide.

Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Immunofluorescent detection of beta Amyloid with MOAB-2 in the subiculim of 1-, 2- and 4- month old 5xFAD mice. Scale bar 20 um (from PMID: 22423893).

Immunohistochemistry: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - A plaque load in neocortex and hippocampus in APP/PS1 mice exposed to differential housing. Representative 10x MOAB-2 staining in hippocampus of APP/PS1 mice for each housing group. Environmental novelty exacerbates stress hormones and Aβ pathology in an Alzheimer's model. Sci Rep (2017)

Immunohistochemistry-Paraffin: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of a formalin fixed paraffin embedded tissue section of human brain (Alzheimers disease, hippocampus) using 1:40 dilution of anti-beta Amyloid antibody (clone MOAB-2). The staining was developed with HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This beta Amyloid antibody specifically stained the cells with Abeta 42/ Abeta aggregates while the normal cells were negative for abeta peptide.

Immunohistochemistry-Frozen: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Mouse brain (cerebral cortex). Red: MOAB-2 antibody staining, Blue: DAPI. Zeiss LSM800, 40x. Image from verified customer review.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, DB, ELISA, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
• Clone: MOAB-2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

beta Amyloid Antibody (MOAB-2) Summary

• Immunogen: This beta Amyloid antibody was developed against recombinant human beta Amyloid 42.
• Epitope: Residues 1-4 of beta Amyloid
• Specificity: Beta Amyloid antibody (MOAB-2) recognizes unaggregated, oligomeric and fibrillar forms of beta Amyloid 42 and unaggregated beta Amyloid 40. Does not detect APP.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: APP
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:5000
  • Dot Blot
  • ELISA 1:100-1:1000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:200-1:500
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 1:200-1:1000
  • Immunohistochemistry Free-Floating
• Application Notes: This beta Amyloid Antibody (MOAB-2) is useful for Immunocytochemistry/Immunofluorescence, ELISA, Immunoprecipitation, Western Blot, and Immunohistochemistry on paraffin-embedded sections. In Western blot, a band can be seen at ~4 kDa, representing the beta Amyloid monomer. Larger bands may also be seen representing the unaggregated, oligomeric, and fibrillar forms of beta Amyloid. For higher beta Amyloid yield in WB, please follow the extraction protocol described in Youmans et al, J Neurosci Methods. 2011 March 15; 196(1): 51-59 (PMID: 21219931). Use in Immunohistochemistry-Free floating reported in scientific literature (PMID 25747037). Use in Dot blot reported in scientific literature (PMID: 22423893). Use in immunoblotting reported in scientific literature (PMID: 28314768).
• Theoretical MW: 5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse and rat. Monkey reactivity reported in scientific literature (PMID: 29241829).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Notes

5xFAD mice = transgenic mice expressing 5 familial Alzheimer's disease mutation. See Oakley H et al, J Neurosci. 2006 Oct 4;26(40):10129-40 (PMID: 17021169) for more information on 5xFAD mice.

Alternate Names for beta Amyloid Antibody (MOAB-2)

• Abeta
• Amyloid beta
• beta-Amyloid Peptide

Background

Beta amyloid, also known as Abeta, is a peptide that varies in length from 39 to 43 amino acids and is processed from the transmembrane protein amyloid precursor protein (APP) by beta-secretase (BACE-1) and gamma-secretase. Beta amyloid [1-40], beta amyloid [1-42], and beta amyloid [1-43] peptides result from cleavage of APP after residues 40, 42, and 43, respectively. The aggregation of beta amyloid monomers produces insoluble oligomers and protofibrils. The longer "stickier" forms of beta amyloid, particularly ABeta42, are known as the main component of amyloid plaques, which are deposits (senile plaques or neuritic plaques) found in the brains of patients with Alzheimer's disease (AD) (1,2). Research indicates that intraneuronal beta amyloid accumulation may be an important proximal neurotoxic event in the pathogenesis of Alzheimer's disease. Abeta deposits have also been reported in the hearts of AD patients (3).

Pyroglutamate amyloid beta peptides (pGlu-Abeta) are N-terminal truncations in which the N-terminal glutamate is cyclized to pyroglutamate resulting in pGlu-Abeta (3-40/42 and 11-40/42) (4). This pyrE modification may have a greater propensity to aggregate under physiological conditions and has been implicated as the molecular species responsible for seeding larger oligomers of amyloid beta.

References

1. Chen GF, Xu TH, Yan Y, Zhou YR, Jiang Y, Melcher K, Xu HE. (2017) Amyloid beta: structure, biology and structure-based therapeutic development. Acta Pharmacol Sin. 38(9):1205-1235. PMID: 28713158

2. De-Paula VJ1, Radanovic M, Diniz BS, Forlenza OV. (2012) Alzheimer's disease. Subcell Biochem. 65:329-52. PMID: 23225010

3. Schaich CL, Maurer MS, Nadkarni NK. (2019) Amyloidosis of the Brain and Heart: Two Sides of the Same Coin? JACC Heart Fail. 7(2):129-131. PMID: 30704604

4. He W, Barrow CJ. (1999) The A beta 3-pyroglutamyl and 11-pyroglutamyl peptides found in senile plaque have greater beta-sheet forming and aggregation propensities in vitro than full-length A beta. Biochemistry. 38(33):10871-7. PMID: 10451383

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta Amyloid Antibody (NBP2-13075)(31)

Publications using NBP2-13075

• FernAndez-PErez EJ, Gallegos S, Armijo-Weingart L et al. Changes in neuronal excitability and synaptic transmission in nucleus accumbens in a transgenic Alzheimer's disease mouse model Sci Rep Nov 11 2020 [PMID: 33177601] (IHC-FrFl, Mouse)
• Hernandez-Rodriguez M, Arciniega-Martinez IM, Garcia-Marin ID et al. Chronic Administration of Scopolamine Increased GSK3betaP9, Beta Secretase, Amyloid Beta, and Oxidative Stress in the Hippocampus of Wistar Rats Mol. Neurobiol. Jul 7 2020 [PMID: 32638218]
  
  Details:
  Citation using the HRP format of this antibody.
• Lee CL, Lin PY, Hsu YW, Pan TM. Monascus-fermented monascin and ankaflavin improve the memory and learning ability in amyloid beta-protein intracerebroventricular-infused rat via the suppression of Alzheimer's disease risk factors. Journal of Functional Foods. 2015 Oct 01 (IHC-Fr, Rat)
  
  Details:
  beta Amyloid antibody used for immunohistochemical staining of rat brain frozen tissue at a dilution of 1:100 (Fig. 7B).
• Acharya A, Stockmann J, Beyer L et al. The Effect of (-)-Epigallocatechin-3-Gallate on the Amyloid-beta Secondary Structure Biophys. J. Jun 10 2020 [PMID: 32579965]
• Voth S, Gwin M, Francis CM et al. Virulent Pseudomonas aeruginosa infection converts antimicrobial amyloids into cytotoxic prions FASEB J. May 15 2020 [PMID: 32413239] (WB, Bacteria)
• Liu XQ, Deng YX, Dai Z et al. Sodium tanshinone IIA sulfonate protects against A alpha 1-42-induced cellular toxicity by modulating A beta-degrading enzymes in HT22 cells Int. J. Biol. Macromol. Feb 6 2020 [PMID: 32035959] (WB, Human)
• He Y, Ruganzu JB, Lin C et al. Tanshinone IIA ameliorates cognitive deficits by inhibiting endoplasmic reticulum stress-induced apoptosis in APP/PS1 transgenic mice Neurochem. Int. Nov 25 2019 [PMID: 31778727] (IHC, Mouse)
• Joseph E, Villalobos-Acosta DMA, Torres-Ramos MA et al. Neuroprotective Effects of Apocynin and Galantamine During the Chronic Administration of Scopolamine in an Alzheimer's Disease Model J. Mol. Neurosci. Nov 25 2019 [PMID: 31768942]
• Prokop S, Miller KR, Labra SR et al. Impact of TREM2 risk variants on brain region-specific immune activation and plaque microenvironment in Alzheimer's disease patient brain samples Acta Neuropathol. Jul 26 2019 [PMID: 31350575] (IHC-P, Human)
• Balczon R, Pittet JF, Wagener BM et al. Infection-induced endothelial amyloids impair memory FASEB J. Jun 18 2019 [PMID: 31211919]
• Tian C, Liu G, Gao L Erythrocytic alpha -Synuclein as a potential biomarker for Parkinson's disease Transl Neurodegener May 15 2019 [PMID: 31123587] (Human)
• El Hajjar J, Chatoo W, Hanna R et al. Heterochromatic genome instability and neurodegeneration sharing similarities with Alzheimer's disease in old Bmi1+/- mice. Sci Rep 2019 Jan 24 [PMID: 30679733] (WB, Mouse)
  
  Details:
  This beta Amyloid antibody was used in Western Blot experiments evaluating the use of aging Bmi1+/- as a model to study novel pathogenic AD-related mechanisms.
• Cristiano C, Volpicelli F, Lippiello P et al. Neutralization of interleukin-17 rescues amyloid-b-induced neuroinflammation and memory impairment. Br. J. Pharmacol. Jan 23 2019 [PMID: 30673121] (WB, Mouse)
• Collins JM, King AE, Woodhouse A et al. Age moderates the effects of traumatic brain injury on beta-amyloid plaque load in APP/PS1 mice. J. Neurotrauma Jan 9 2019 [PMID: 30623730] (IHC-FrFl, Mouse)
• Kim DH, Lee D, Lim H et al. Effect of growth differentiation factor-15 secreted by human umbilical cord blood-derived mesenchymal stem cells on amyloid beta levels in invitro and invivo models of Alzheimer's disease. Biochem. Biophys. Res. Commun. Sep 14 2018 [PMID: 30224067] (ICC/IF, Human, Mouse)
• Woodhouse A, Fernandez-Martos CM, Atkinson RAK et al. Repeat propofol anesthesia does not exacerbate plaque deposition or synapse loss in APP/PS1 Alzheimer's disease mice. BMC Anesthesiol 2018 Apr 25 [PMID: 29699479]
• Kim D, Lim H, Lee D et al. Thrombospondin-1 secreted by human umbilical cord blood-derived mesenchymal stem cells rescues neurons from synaptic dysfunction in Alzheimer's disease model. Sci Rep 2018 Jan 10 [PMID: 29321508] (WB)
  
  Details:
  This beta Amyloid antibody was used as a loading control in Western Blot experiments supporting that hUCB-MSCs may protect from hippocampus synaptic density loss in AD models.
• Paspalas CD, Carlyle BC, Leslie S et al. The aged rhesus macaque manifests Braak stage III/IV Alzheimer's-like pathology. Alzheimers Dement 2017 Dec 11 [PMID: 29241829] (ICC/IF, Monkey)
• Liu Y, Hanson K, McCormack G et al. Enhanced anti-amyloid effect of combined leptin and pioglitazone in APP/PS1 transgenic mice bioRxiv Jun 25 2020 (Mouse)
• Muthukumaran K, Kanwar A, Vegh C et al. Ubisol-Q10 (a Nanomicellar Water-Soluble Formulation of CoQ10) Treatment Inhibits Alzheimer-Type Behavioral and Pathological Symptoms in a Double Transgenic Mouse (TgAPEswe, PSEN1dE9) Model of Alzheimers Disease Journal of Alzheimer's Disease 2017 Nov 28 [PMID: 29154270] (IHC, Mouse)
• Lee DY, Hwang CJ, Choi JY et al. KRICT-9 inhibits neuroinflammation, amyloidogenesis and memory loss in Alzheimer's disease models. Oncotarget. 2017 Sep 15 [PMID: 28978145] (ICC/IF, Mouse)
• Stuart KE, King AE, Fernandez-Martos CM et al. Environmental novelty exacerbates stress hormones and AB pathology in an Alzheimer's model. Sci Rep. 2017 Jun 05 [PMID: 28584278] (ICC/IF, Mouse)
• Balczon R, Morrow KA, Zhou C et al. Pseudomonas aeruginosa infection liberates transmissible, cytotoxic prion amyloids. FASEB J. Mar 17 2017 [PMID: 28314768]
• Stuart KE, King AE, Fernandez-Martos CM et al. Mid-life environmental enrichment increases synaptic density in CA1 in a mouse model of Ab-associated pathology and positively influences synaptic and cognitive health in healthy ageing. J. Comp. Neurol. Dec 17 2016 [PMID: 27987205]
• Yu YW, Hsieh TH, Chen KY et al. Glucose-dependent insulinotropic polypeptide ameliorates mild traumatic brain injury-induced cognitive and sensorimotor deficits and neuroinflammation in rats J. Neurotrauma Mar 14 2016 [PMID: 26972789] (WB, Rat)
• Kim JA, Yun HM, Jin P et al. Inhibitory effect of a 2,4-bis(4-hydroxyphenyl)but-2-enal diacetate on neuro-inflammatory reactions via inhibition of STAT1 and STAT3 activation in cultured astrocytes and microglial BV-2 cells. Neuropharmacology 2013 Jul 23 [PMID: 23891616] (ICC/IF, Rat)
• Youmans KL, Tai LM, Nwabuisi-Heath E et al. APOE4-specific Changes in A? Accumulation in a New Transgenic Mouse Model of Alzheimer Disease J Biol Chem 2012 Dec 7 [PMID: 23060451] (ELISA, IHC, Human)
• Tai, LM, Youmans, K et al. Intraneuronal Abeta detection in 5xFAD mice by a new Abeta-specific antibody Molecular Neurodegeneration 2012 [PMID: 22423893] (ICC/IF, WB, IP, DB, Mouse)
• El Hajjar J, Chatoo W, Hanna R, Nkanza P. Heterochromatic genome instability and neurodegeneration sharing similarities with Alzheimer's disease in old Bmi1 +/- mice Scientific reports Jul 11 1905 (WB, Human)
• Collins JM, King AE, Woodhouse A et al. The effect of focal brain injury on beta-amyloid plaque deposition, inflammation and synapses in the APP/PS1 mouse model of Alzheimer's disease Exp. Neurol. 2015 Mar 04 [PMID: 25747037] (IHC-FrFl, Mouse)
  
  Details:
  beta Amyloid antibody (clone MOAB-2) used for IHC immunostaining on free floating brain sections from experiments involving focal brain injury in 3 or 9 months old male APPSWE/PSENdE9 (APP/PS1) transgenic mice (a murine model of Alzheimer's disease, Jankowsky et al. 2001 Biomol Eng. 17: 157-165 ) and C57BL/6 wild-type mice. The assay involved 4% paraformaldehyde-PBS fixation, 8 minutes of antigen retrieval with 88% formic acid at RT, permeablization 3X10 minutes each using 0.25% Triton-X-100-PBS, 15 minutes blocking with Dako serum-free protein block, primary used at 1:2000 dilution with ON 4C incubation, detection using Alexa-fluorophore conjugated secondary antibodies (see full text for detailed protocol and staining images).

Reviews for beta Amyloid Antibody (NBP2-13075) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, 5xFAD mouse.

reviewed by: Anonymous WB Mouse 12/11/2019

Summary

• Application: Western Blot
• Sample Tested: neonatal cardiac myocytes
• Species: Mouse
• Lot: F-3

reviewed by: Lei Yao IHC-Fr 5xFAD mouse 09/22/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Brain (cerebral cortex)
• Species: 5xFAD mouse
• Lot: E-1

FAQs for beta Amyloid Antibody (NBP2-13075). (Showing 1 - 2 of 2 FAQs).

1. Does your antibody beta Amyloid Antibody (MOAB-2) # NBP2-13075 work on frozen sections for histology?
   • This antibody has never been tested on frozen sections. It has been validated in ICC, IP and IHC on paraffin-embedded tissues, so it may work on frozen sections. While we cannot guarantee that it will work on frozen sections, if you would like to test NBP2-13075 in an untested application and share your results with us, I can recommend our Innovator's Reward Program. Our Innovator's Reward program was created to allow researchers the opportunity to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply submit an online review detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Reviews may also be submitted by emailing innovators@novusbio.com.
2. Please provide the epitope region information for NBP2-13075 Beta Amyloid Antibody.
   • The epitope is a.a. 672 - 675 and please use https://www.uniprot.org/uniprot/P05067#sequences to check all important information.

Bioinformatics

• Gene Symbol: APP
• Entrez:
  • 351(Human)
• Uniprot:
  • P05067()