beta Amyloid Antibody (MOAB-2) - BSA Free

Images

 
IHC analysis of beta Amyloid on normal mouse brain (left) and 5xFAD mouse brain (right) using DAB with hematoxylin counterstain. The MOAB-2 antibody was used at 1:20 on normal mouse brain and at 1:400 on 5xFAD mouse ...read more
Analysis of beta Amyloid (MOAB-2) antibody in (1) 100 pmole beta Amyloid 42, (2) 5xFAD mouse brain homogenate Repetition 1 and (3) 5xFAD mouse brain homogenate Repetition 2.
Western blot analysis in cell lysates from HEK-APP SWE/BACE1 cells probed with an antibody against the C-terminus of APP (Lanes 1 and 2) and beta Amyloid (MOAB-2, Lanes 3 and 4). Beta Amyloid (MOAB-2) does not detect ...read more
IHC analysis of a formalin fixed paraffin embedded tissue section of human brain (Alzheimers disease, hippocampus) using 1:200 dilution of anti-beta Amyloid antibody (clone MOAB-2). The staining was developed with HRP ...read more
Immunofluorescent detection of beta Amyloid with MOAB-2 in the subiculim of 1-, 2- and 4- month old 5xFAD mice. Scale bar 20 um (from PMID: 22423893).
A plaque load in neocortex and hippocampus in APP/PS1 mice exposed to differential housing. Representative 10x MOAB-2 staining in hippocampus of APP/PS1 mice for each housing group. Image collected and cropped by ...read more
IHC analysis of a formalin fixed paraffin embedded tissue section of human brain (Alzheimers disease, hippocampus) using 1:40 dilution of anti-beta Amyloid antibody (clone MOAB-2). The staining was developed with HRP ...read more
Mouse brain (cerebral cortex). Red: MOAB-2 antibody staining, Blue: DAPI. Zeiss LSM800, 40x. Image from verified customer review.
No difference in Abeta plaque load between propofol (n = 8) and vehicle (n = 6) treated APP/PS1 mice. a An example image of Abeta plaque immunoreactivity in the cortex of an APP/PS1 mouse following repeated propofol ...read more
Abeta plaque load in neocortex and hippocampus in APP/PS1 mice exposed to differential housing. (F) Representative 10x MOAB-2 staining in hippocampus of APP/PS1 mice for each housing group. Image collected and cropped ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ba, PmSpecies Glossary
Applications WB, DB, ELISA, ICC/IF, IHC, IP, WB, IHC
Clone
MOAB-2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

beta Amyloid Antibody (MOAB-2) - BSA Free Summary

Immunogen
This beta Amyloid antibody was developed against recombinant human beta Amyloid 42.
Epitope
Residues 1-4 of beta Amyloid
Specificity
Beta Amyloid antibody (MOAB-2) recognizes unaggregated, oligomeric and fibrillar forms of beta Amyloid 42 and unaggregated beta Amyloid 40. Does not detect APP.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
APP
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dot Blot reported in scientific literature (PMID 22423893)
  • ELISA 1:100-1:1000
  • Immunoblotting reported in scientific literature (PMID 28314768)
  • Immunocytochemistry/ Immunofluorescence 1:200-1:500
  • Immunohistochemistry Free-Floating reported in scientific literature (PMID 25747037)
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 1:200-1:1000
  • Western Blot 1:1000-1:5000
Application Notes
In Western blot, a band can be seen at ~4 kDa, representing the beta Amyloid monomer. Larger bands may also be seen representing the unaggregated, oligomeric, and fibrillar forms of beta Amyloid. For higher beta Amyloid yield in WB, please follow the extraction protocol described in Youmans et al, J Neurosci Methods. 2011 March 15; 196(1): 51-59 (PMID: 21219931).
Theoretical MW
5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP2-13075 in the following applications:

Publications
Read Publications using
NBP2-13075 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:35401818). Monkey reactivity reported in scientific literature (PMID: 29241829). Use in Bacteria reported in scientific literature (PMID:32413239).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for beta Amyloid Antibody (MOAB-2) - BSA Free

  • Abeta
  • Amyloid beta
  • beta-Amyloid Peptide

Background

Beta amyloid, also known as Abeta, is a peptide that varies in length from 39 to 43 amino acids and is processed from the transmembrane protein amyloid precursor protein (APP) by beta-secretase (BACE-1) and gamma-secretase. Beta amyloid [1-40], beta amyloid [1-42], and beta amyloid [1-43] peptides result from cleavage of APP after residues 40, 42, and 43, respectively. The aggregation of beta amyloid monomers produces insoluble oligomers and protofibrils. The longer "stickier" forms of beta amyloid, particularly ABeta42, are known as the main component of amyloid plaques, which are deposits (senile plaques or neuritic plaques) found in the brains of patients with Alzheimer's disease (AD) (1,2). Research indicates that intraneuronal beta amyloid accumulation may be an important proximal neurotoxic event in the pathogenesis of Alzheimer's disease. Abeta deposits have also been reported in the hearts of AD patients (3).

Pyroglutamate amyloid beta peptides (pGlu-Abeta) are N-terminal truncations in which the N-terminal glutamate is cyclized to pyroglutamate resulting in pGlu-Abeta (3-40/42 and 11-40/42) (4). This pyrE modification may have a greater propensity to aggregate under physiological conditions and has been implicated as the molecular species responsible for seeding larger oligomers of amyloid beta.

References

1. Chen GF, Xu TH, Yan Y, Zhou YR, Jiang Y, Melcher K, Xu HE. (2017) Amyloid beta: structure, biology and structure-based therapeutic development. Acta Pharmacol Sin. 38(9):1205-1235. PMID: 28713158

2. De-Paula VJ1, Radanovic M, Diniz BS, Forlenza OV. (2012) Alzheimer's disease. Subcell Biochem. 65:329-52. PMID: 23225010

3. Schaich CL, Maurer MS, Nadkarni NK. (2019) Amyloidosis of the Brain and Heart: Two Sides of the Same Coin? JACC Heart Fail. 7(2):129-131. PMID: 30704604

4. He W, Barrow CJ. (1999) The A beta 3-pyroglutamyl and 11-pyroglutamyl peptides found in senile plaque have greater beta-sheet forming and aggregation propensities in vitro than full-length A beta. Biochemistry. 38(33):10871-7. PMID: 10451383

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta Amyloid Antibody (NBP2-13075)(63)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bacteria, Monkey.

We have publications tested in 10 applications: Cytometric Bead Assay Standard, ELISA, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-FrFl, IHC-P, IP, WB.


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Cytometric Bead Assay Standard
(2)
ELISA
(1)
ICC/IF
(8)
IF/IHC
(10)
IHC
(3)
IHC-Fr
(6)
IHC-FrFl
(3)
IHC-P
(3)
IP
(1)
WB
(13)
All Applications
Filter By Species
Human
(7)
Mouse
(27)
Rat
(5)
Bacteria
(1)
Monkey
(1)
All Species
Showing Publications 1 - 10 of 63. Show All 63 Publications.
Publications using NBP2-13075 Applications Species
Tiwari V, Singh J, Tiwari P Et al. ACE2/ANG-(1-7)/Mas Receptor Axis Activation Prevents Inflammation, Glial Activation And Cognitive Decline In a Rat Model of STZ Induced Impairment of Learning and Memory Research Square 2021-10-26 (WB, Mouse) WB Mouse
Marinho D, Ferreira IL, Lorenzoni R et al. Reduction of class I histone deacetylases ameliorates ER-mitochondria cross-talk in Alzheimer's disease Aging cell 2023-06-26 [PMID: 37358017] (Mouse) Mouse
Chae CW, Yoon JH, Lim JR et al. TRIM16-mediated lysophagy suppresses high-gluocse-accumulated neuronal A? Autophagy 2023-06-25 [PMID: 37357416]
Kim MY, Kim S, Lee J et al. Lignan-Rich Sesame (Sesamum indicum L.) Cultivar Exhibits In Vitro Anti-Cholinesterase Activity, Anti-Neurotoxicity in Amyloid-beta Induced SH-SY5Y Cells, and Produces an In Vivo Nootropic Effect in Scopolamine-Induced Memory Impaired Mice Antioxidants (Basel, Switzerland) 2023-05-17 [PMID: 37237976] (WB, Mouse) WB Mouse
Li JM, Hu T, Zhou XN et al. The involvement of NLRP3 inflammasome in CUMS-induced AD-like pathological changes and related cognitive decline in mice Journal of neuroinflammation 2023-05-10 [PMID: 37165444] (IHC, Mouse) IHC Mouse
Panes-Fernandez J, Godoy PA, Gavilan J et al. TG2 promotes amyloid beta aggregates: Impact on ER-mitochondria crosstalk, calcium homeostasis and synaptic function in Alzheimer's disease Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023-03-27 [PMID: 36989728] (ICC/IF, IHC-Fr, Mouse) ICC/IF, IHC-Fr Mouse
Show All 63 Publications.

Reviews for beta Amyloid Antibody (NBP2-13075) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, 5xFAD mouse.

Reviews using NBP2-13075:
Filter by Applications
WB
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
5xFAD mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot beta Amyloid NBP2-13075
Enlarge
4
reviewed by:
Verified Customer
WB Mouse 12/11/2019
View

Summary

ApplicationWestern Blot
Sample Testedneonatal cardiac myocytes
SpeciesMouse
LotF-3
Immunohistochemistry-Frozen beta Amyloid NBP2-13075
Enlarge
5
reviewed by:
Lei Yao
IHC-Fr 5xFAD mouse 09/22/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedBrain (cerebral cortex)
Species5xFAD mouse
LotE-1

Product General Protocols

View specific protocols for beta Amyloid Antibody (NBP2-13075): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta Amyloid Antibody (NBP2-13075). (Showing 1 - 2 of 2 FAQs).

  1. Does your antibody beta Amyloid Antibody (MOAB-2) # NBP2-13075 work on frozen sections for histology?
    • This antibody has never been tested on frozen sections. It has been validated in ICC, IP and IHC on paraffin-embedded tissues, so it may work on frozen sections. While we cannot guarantee that it will work on frozen sections, if you would like to test NBP2-13075 in an untested application and share your results with us, I can recommend our Innovator's Reward Program. Our Innovator's Reward program was created to allow researchers the opportunity to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply submit an online review detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Reviews may also be submitted by emailing innovators@novusbio.com.
  2. Please provide the epitope region information for NBP2-13075 Beta Amyloid Antibody.
    • The epitope is a.a. 672 - 675 and please use https://www.uniprot.org/uniprot/P05067#sequences to check all important information.

Secondary Antibodies

 

Isotype Controls

Additional beta Amyloid Products

Research Areas for beta Amyloid Antibody (NBP2-13075)

Find related products by research area.

Blogs on beta Amyloid.

Mechanisms of Neurodegeneration: Neuroinflammation and microglial activation
By Michalina Hanzel, PhDIn this final instalment of our three blog-posts series on major cellular mechanisms responsible for neurodegenerative disorders, we will explore the processes of neuroinflammation and microgl...  Read full blog post.

Immunity’s flipside: Microglia promote Alzheimer’s pathology during inflammation
By Jamshed Arslan Pharm.D. Microglia are brain's macrophages. In Alzheimer's disease (AD), microglia clear up protein aggregates called amyloid beta plaques. The connection between immune activation and AD is unclea...  Read full blog post.

Beta Amyloid (MOAB2) and the link between traumatic brain injury and Alzheimer’s disease
An epidemiological association between traumatic brain injury (TBI) and Alzheimer's disease (AD) has long been established.  Interestingly, an increase in beta amyloid  (one hallmark of AD) directly following TBI has been observed.  In fact, it h...  Read full blog post.

Analysis of Total & pSer724 IRE1 alpha, the Sensor of ER Stress
Inositol-requiring protein 1/IRE1 alpha (also called Endoplasmic Reticulum to Nucleus Signaling 1/ERN1; predicted mol wt 110 kDa) is a serine-threonine protein kinase/endoribonuclease which plays a highly critical role in unfolded protein response/U...  Read full blog post.

Beta Amyloid Neurotoxicity and Alzheimer's Disease
A major histopathological hallmark of Alzheimer's disease (AD) is the presence of amyloid deposits in the parenchyma of the amygdala, hippocampus, and neocortex. The principal component of amyloid is beta amyloid (AB). The pathologic accumulation of A...  Read full blog post.

Using Amyloid beta peptides in Alzheimer's Disease Immunization
Amyloid beta (AB) peptide has a central role in the neurodegeneration of Alzheimer's disease (AD). Immunization of AD transgenic mice with AB-42 peptide reduces both the spatial memory impairments and AD-like neuropathologic changes.Therapeutic im...  Read full blog post.

Synaptophysin and Dementing Disorders
 Synaptophysin (a presynaptic vesicle protein) is an integral membrane glycoprotein originally isolated from presynaptic vesicles of bovine neurons. Synaptophysin is found in all nerve terminals and synaptophysin measurements have been used to quantif...  Read full blog post.

Amyloid beta and Methionine Sulfoxide Related to Abeta 42 Antibody and Abeta 40 Antibody
By Eric NeeleyAlzheimer's disease is a devastating neurodegenerative illness characterized by the formation of plaques, tangles, and eventually synaptic loss. Amyloid beta (A beta ) is the processed form of the Amyloid precursor protein (APP), and whose ...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Verified Customer
12/11/2019
Application: WB
Species: Mouse

Lei Yao
09/22/2018
Application: IHC-Fr
Species: 5xFAD mouse

Bioinformatics

Gene Symbol APP
Entrez
Uniprot