MAP2 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: MAP2 Antibody [NB300-213] - Mouse primary cultures stained with MAP2 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: MAP2 Antibody [NB300-213] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to microtubule associated protein 2 (MAP2), ...read more
Western Blot: MAP2 Antibody [NB300-213] - Western blot analysis of whole brain tissue lysates using chicken pAb to microtubule associated protein 2 (MAP2), NB300-213, dilution 1:50,000 in green: [1] protein standard ...read more
Immunocytochemistry/ Immunofluorescence: MAP2 Antibody [NB300-213] - PEA decreases astrocyte activation in organotypic cultures of rat hippocampi and rescues neuronal CA3 damage caused by Abeta challenge. ...read more
Immunocytochemistry/ Immunofluorescence: MAP2 Antibody [NB300-213] - PEA inhibits astroglial proliferation and reduces neuronal loss in mixed neuroglia co-cultures exposed to Abeta. Abeta-challenged (1 ug/ml) ...read more
Immunohistochemistry-Paraffin: MAP2 Antibody [NB300-213] - Differential and neuron-specific Oxtr expression in mouse hippocampus. Immunohistochemical mapping of Venus expression in coronal sections of the rostral, ...read more
Immunocytochemistry/ Immunofluorescence: MAP2 Antibody [NB300-213] - Human DA neurons 28 DIV stained with MAP2 antibody (blue). Image from verified customer review.
Immunohistochemistry-Paraffin: MAP2 Antibody [NB300-213] - Oxytocin receptors (Oxtr) are expressed in mouse hippocampus. Confocal images of Oxtr-expressing neurons in the CA1 subfields of the medial and caudal ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, KD
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated

Order Details

MAP2 Antibody Summary

Immunogen
This MAP2 antibody was developed against a mix of recombinant human construct of projection domain sequences, amino acids 377-1505: Prot-r-MAP2-P1, Prot-r-MAP2-P2 and Prot-r-MAP2-P3.
Localization
Cytoskeleton
Marker
Neuronal Dendritic Marker
Specificity
This antibody was raised against recombinant constructs of the entire human projection domain, and so recognizes the high molecular MAP2 forms, MAP2A and MAP2B.
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
MAP2
Purity
N/A N/A
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000-1:10000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Frozen 1:1000-1:5000
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
Application Notes
Western blot bands can be seen at approximately 280 kDa, representing MAP2a and MAP2b. Bands may also be seen at approximately 70 kDa, representing MAP2c. Use in Immunohistochemistry-Frozen reported in multiple pieces of scientific literature. IHC-Fr reported in scientific literature (PMID: 30782076). Knockdown data from PMID 32294442.
Theoretical MW
199 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 4 Reviews rated 5
using
NB300-213 in the following applications:

Publications
Read Publications using
NB300-213 in the following applications:

  • 34 publications
  • IF
    1 publication
  • IHC
    7 publications
  • 4 publications
  • KD
    1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS plus 0.02 mM sodium azide.
Preservative
0.02% Sodium Azide
Purity
N/A N/A

Alternate Names for MAP2 Antibody

  • DKFZp686I2148
  • MAP2
  • MAP-2
  • MAP2A
  • MAP2B
  • MAP2C
  • Microtubule Associated Protein 2
  • microtubule-associated protein 2
  • MTAP2

Background

Microtubule-associated protein 2 also known as MAP2 (theoretical molecular weight 280 kDa) belongs to the MAP2/Tau family which in addition to the two neuronal forms MAP2 and Tau also includes MAP4 (1). For MAP2, four different isoforms have been identified which are produced through alternative splicing including MAP2a, MAP2b, MAP2c and MAP2d (1). MAP2 isoforms contain multiple microtubule-binding domains located near the carboxy terminal, a variably sized amino-terminal projection domain, and a conserved protein kinase A binding domain. MAP2c and MAP2d isoforms have a shorter amino-terminal projection domain, lower molecular weight and are identifiable at ~70 kDa in SDS-PAGE analysis (2,3). MAP2's carboxy terminal domain supports interactions with microtubules, actin, and intermediate filaments (2).

MAP2 isoforms are developmentally regulated and differentially expressed in neurons and some glia. MAP2c is predominantly expressed in the developing brain while the other isoforms are expressed in the adult brain. The distribution of MAP2 isoforms also varies, with MAP2a and MAP2b predominantly localized to dendrites, while MAP2c is also found in axons. Lastly, the expression of MAP2d is not limited to neurons and may be found in glia, specifically oligodendrocytes (1, 2). MAP2 isoforms associate with microtubules and mediate their interaction with actin filaments thereby playing a critical role in organizing the microtubule-actin network. In neurons, MAP2 isoforms are implicated in different processes including neurite initiation, elongation and stabilization as well as axon and dendrite formation (2). Knockout of MAP expression in animal models results in a variety of functional and structural brain defects according to the isoform affected (e.g., reduced LTP and LTD, reduced myelination, absence of corpus collosum, motor system malfunction, abnormal hippocampal dendritic morphology, abnormal synaptic plasticity) (4).

References

1. Dehmelt, L., & Halpain, S. (2005). The MAP2/Tau family of microtubule-associated proteins. Genome Biology. https://doi.org/10.1186/gb-2004-6-1-204

2. Mohan, R., & John, A. (2015). Microtubule-associated proteins as direct crosslinkers of actin filaments and microtubules. IUBMB Life. https://doi.org/10.1002/iub.1384

3. Shafit-Zagardo, B., & Kalcheva, N. (1998). Making sense of the multiple MAP-2 transcripts and their role in the neuron. Molecular Neurobiology. https://doi.org/10.1007/BF02740642

4. Tortosa, E., Kapitein, L. C., & Hoogenraad, C. C. (2016). Microtubule organization and microtubule-associated proteins (MAPs). In Dendrites: Development and Disease. https://doi.org/10.1007/978-4-431-56050-0_3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MAP2 Antibody (NB300-213)(51)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ICC/IF, IF, IHC, IHC-Fr, KD, WB.


Filter By Application
ICC/IF
(34)
IF
(1)
IHC
(7)
IHC-Fr
(4)
KD
(1)
WB
(2)
All Applications
Filter By Species
Human
(15)
Mouse
(22)
Rat
(11)
All Species
Showing Publications 1 - 10 of 51. Show All 51 Publications.
Publications using NB300-213 Applications Species
Szczurkowska J, Lee SI, Guo A et al. A Localized Scaffold for cGMP Increase Is Required for Apical Dendrite Development Cell Rep Apr 14 2020 [PMID: 32294442] (ICC/IF, KD, Human) ICC/IF, KD Human
Ripamonti S, Shomroni O, Rhee JS et al. SUMOylation controls the neurodevelopmental function of the transcription factor Zbtb20 J. Neurochem. Mar 31 2020 [PMID: 32233089] (ICC/IF, Human) ICC/IF Human
Maus L, Lee C, Altas B et Al. Ultrastructural Correlates of Presynaptic Functional Heterogeneity in Hippocampal Synapses Cell Rep Mar 17 2020 [PMID: 32187536] (IHC, Mouse) IHC Mouse
Kim BW, Jeong YE, Wong M, Martin LJ DNA damage accumulates and responses are engaged in human ALS brain and spinal motor neurons and DNA repair is activatable in iPSC-derived motor neurons with SOD1 mutations Acta Neuropathol Commun Jan 31 2020 [PMID: 32005289] (WB, Human) WB Human
Toh MF, Brooks JM, Strassmaier T et al. Application of High-Throughput Automated Patch-Clamp Electrophysiology to Study Voltage-Gated Ion Channel Function in Primary Cortical Cultures SLAS Discov Jan 31 2020 [PMID: 32003306] (WB) WB
Bonazzi S, Goold CP, Gray A et al. Discovery of a Brain-Penetrant ATP-Competitive Inhibitor of the Mechanistic Target of Rapamycin (mTOR) for CNS Disorders J. Med. Chem. Feb 13 2020 [PMID: 31955578]
HyvArinen T, Hyysalo A, Kapucu FE et al. Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures Sci Rep Nov 20 2019 [PMID: 31748598] (ICC/IF, Rat) ICC/IF Rat
Sen T, Saha P, Gupta R, et al. Aberrant ER-stress induced neuronal-IFN beta elicits white matter injury due to microglial activation and T-cell infiltration after TBI. J Neurosci. [PMID: 31694961] (IF, Mouse) IF Mouse
Subramanian J, Michel K, Benoit M, Nedivi E CPG15/Neuritin Mimics Experience in Selecting Excitatory Synapses for Stabilization by Facilitating PSD95 Recruitment Cell Rep Aug 6 2019 [PMID: 31390571] (ICC/IF, Mouse, Human) ICC/IF Mouse, Human
Cuesta Torres LF, Zhu W, Ohrling G et al. An Autaptic Culture System for Standardized Analyses of iPSC-Derived Human Neurons Cell Rep May 14 2019 [PMID: 31091457] (ICC/IF, Mouse, Rat) ICC/IF Mouse, Rat
Show All 51 Publications.

Reviews for MAP2 Antibody (NB300-213) (4) 54

Average Rating: 5
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NB300-213:
Filter by Applications
ICC
(2)
IF
(1)
IHC-Fr
(1)
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Filter by Species
Human
(1)
Mouse
(1)
Rat
(2)
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Images Ratings Applications Species Date Details
Immunocytochemistry MAP2 NB300-213
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reviewed by:
Karthik Krishnamurthy
ICC Rat 11/01/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedCultured motor neurons
SpeciesRat
Lot7225-9

Comments

CommentsUsed at a dilution of 1:2500. incubated at 4 degrees overnight. Robust staining
Immunocytochemistry MAP2 NB300-213
Enlarge
reviewed by:
Lingling Yang
ICC Mouse 11/22/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse primary cultures
SpeciesMouse

Comments

Comments1:1000 dilution
Immunofluorescence MAP2 NB300-213
Enlarge
reviewed by:
Anonymous
IF Human 01/22/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedRat and Human.
SpeciesHuman

Blocking

Blocking Details2%BSA, 2% Donekey serum, 0.1% Triton X100

Primary Anitbody

Dilution Ratio1:2000 in block

Secondary Antibody

Secondary DescriptionDonkey anti Chick-Alexa 647
Secondary Manufacturer Cat#JacksonImmuno
Secondary Concentration1:800

Details

Detection NotesImaged on Leica SP5 confocal microscope
Fixation DetailsPFA/MetOH fixed.
Wash DescriptionPBS 3X
 
reviewed by:
Michael Olive
IHC-Fr Rat 12/07/2010
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRat brain tissue
SpeciesRat
CommentsGreat staining patterns!

Blocking

Blocking DetailsBlocking Buffer: 5% donkey serum, Blocking Time: 2 hours, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1:1000, Incubation Dilution Buffer: PBS + 0.1% Tween + 5% donkey serum, Incubation Time: overnight, Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Dylight488 donkey anti-rabbit, Secondary Ab Dilution: 1:500

Details

Detection NotesDetection Method: epifluorescence
Fixation DetailsSample Preparation: OCT embedded Cryosection

Comments

CommentsGreat staining patterns!

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MAP2 Antibody (NB300-213). (Showing 1 - 3 of 3 FAQs).

  1. Can you please tell me the country of origin for antibody NB300-213, chicken anti-MAP2?
    • Our product NB300-213 originates in the United States.
  2. Would you be able to share the concentration of NB300-213 from Lot# 7214, and the current lot of this antibody?
    • This antibody is a chicken polyclonal that has been precipitated therefore a concentration is irrelevant. This is because, unlike an affinity purified antibody, there will be other proteins that absorb at 280 that are not the specific antibody to the protein target. The concentration is usually about 20mg/ml. We currently are on Lot# 7225-3.
  3. I was using your product NB300-213 for MAP2 immunofluorescence, I did many times, which showed a beaded signal consistently unlike the picture presented on the product datasheet,could you give me some advice?
    • I looked at the images, and I think that the MAP2 antibody is working fine. The beading of the dendrites is likely due to some problem with fixation, which resulted in some damage to the neurons. MAP2 is bound to microtubules which are very labile, rapidly breaking down in the cold or if the cells are metabolically or mechanically challenged. When this happens, the dendrites take up a beaded appearance, as in the images. We routinely use a fixation protocol in which cells are fixed briefly in formalin at room temperature, which stabilizes the microtubules, followed by cold methanol, which helps to permeabilize the cells.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for MAP2 Antibody (NB300-213)

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Blogs on MAP2.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.


  Read full blog post.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

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Recent Reviews

5
5
4
4
0
3
0
2
0
1
0

Karthik Krishnamurthy
11/01/2017
Application: ICC
Species: Rat

Lingling Yang
11/22/2016
Application: ICC
Species: Mouse

Anonymous
01/22/2016
Application: IF
Species: Human

Bioinformatics

Gene Symbol MAP2
Entrez
Uniprot