MAP2 Antibody Summary
This MAP2 antibody was developed against a mix of recombinant human construct of projection domain sequences, amino acids 377-1505: Prot-r-MAP2-P1, Prot-r-MAP2-P2 and Prot-r-MAP2-P3.
Neuronal Dendritic Marker
This antibody was raised against recombinant constructs of the entire human projection domain, and so recognizes the high molecular MAP2 forms, MAP2A and MAP2B.
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- Western Blot 1:10000-1:20000
- Immunocytochemistry/Immunofluorescence 1:5000-1:10000
- Immunohistochemistry 1:1000-1:5000
- Immunohistochemistry-Frozen 1:1000-1:5000
- Knockdown Validated
Western blot bands can be seen at approximately 280 kDa, representing MAP2a and MAP2b. Bands may also be seen at approximately 70 kDa, representing MAP2c. Use in Immunohistochemistry-Frozen reported in multiple pieces of scientific literature. IHC-Fr reported in scientific literature (PMID: 30782076). Knockdown data from PMID 32294442.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
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NB300-213 in the following applications:
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS plus 0.02 mM sodium azide.
0.02% Sodium Azide
Alternate Names for MAP2 Antibody
- Microtubule Associated Protein 2
- microtubule-associated protein 2
Microtubule-associated protein 2 also known as MAP2 (theoretical molecular weight 280 kDa) belongs to the MAP2/Tau family which in addition to the two neuronal forms MAP2 and Tau also includes MAP4 (1). For MAP2, four different isoforms have been identified which are produced through alternative splicing including MAP2a, MAP2b, MAP2c and MAP2d (1). MAP2 isoforms contain multiple microtubule-binding domains located near the carboxy terminal, a variably sized amino-terminal projection domain, and a conserved protein kinase A binding domain. MAP2c and MAP2d isoforms have a shorter amino-terminal projection domain, lower molecular weight and are identifiable at ~70 kDa in SDS-PAGE analysis (2,3). MAP2's carboxy terminal domain supports interactions with microtubules, actin, and intermediate filaments (2).
MAP2 isoforms are developmentally regulated and differentially expressed in neurons and some glia. MAP2c is predominantly expressed in the developing brain while the other isoforms are expressed in the adult brain. The distribution of MAP2 isoforms also varies, with MAP2a and MAP2b predominantly localized to dendrites, while MAP2c is also found in axons. Lastly, the expression of MAP2d is not limited to neurons and may be found in glia, specifically oligodendrocytes (1, 2). MAP2 isoforms associate with microtubules and mediate their interaction with actin filaments thereby playing a critical role in organizing the microtubule-actin network. In neurons, MAP2 isoforms are implicated in different processes including neurite initiation, elongation and stabilization as well as axon and dendrite formation (2). Knockout of MAP expression in animal models results in a variety of functional and structural brain defects according to the isoform affected (e.g., reduced LTP and LTD, reduced myelination, absence of corpus collosum, motor system malfunction, abnormal hippocampal dendritic morphology, abnormal synaptic plasticity) (4).
1. Dehmelt, L., & Halpain, S. (2005). The MAP2/Tau family of microtubule-associated proteins. Genome Biology. https://doi.org/10.1186/gb-2004-6-1-204
2. Mohan, R., & John, A. (2015). Microtubule-associated proteins as direct crosslinkers of actin filaments and microtubules. IUBMB Life. https://doi.org/10.1002/iub.1384
3. Shafit-Zagardo, B., & Kalcheva, N. (1998). Making sense of the multiple MAP-2 transcripts and their role in the neuron. Molecular Neurobiology. https://doi.org/10.1007/BF02740642
4. Tortosa, E., Kapitein, L. C., & Hoogenraad, C. C. (2016). Microtubule organization and microtubule-associated proteins (MAPs). In Dendrites: Development and Disease. https://doi.org/10.1007/978-4-431-56050-0_3
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Product General Protocols
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FAQs for MAP2 Antibody (NB300-213). (Showing 1 - 3 of 3 FAQs).
Can you please tell me the country of origin for antibody NB300-213, chicken anti-MAP2?
- Our product NB300-213 originates in the United States.
Would you be able to share the concentration of NB300-213 from Lot# 7214, and the current lot of this antibody?
- This antibody is a chicken polyclonal that has been precipitated therefore a concentration is irrelevant. This is because, unlike an affinity purified antibody, there will be other proteins that absorb at 280 that are not the specific antibody to the protein target. The concentration is usually about 20mg/ml. We currently are on Lot# 7225-3.
I was using your product NB300-213 for MAP2 immunofluorescence, I did many times, which showed a beaded signal consistently unlike the picture presented on the product datasheet,could you give me some advice?
- I looked at the images, and I think that the MAP2 antibody is working fine. The beading of the dendrites is likely due to some problem with fixation, which resulted in some damage to the neurons. MAP2 is bound to microtubules which are very labile, rapidly breaking down in the cold or if the cells are metabolically or mechanically challenged. When this happens, the dendrites take up a beaded appearance, as in the images. We routinely use a fixation protocol in which cells are fixed briefly in formalin at room temperature, which stabilizes the microtubules, followed by cold methanol, which helps to permeabilize the cells.
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