MAP2 Antibody

Immunocytochemistry/Immunofluorescence: MAP2 Antibody [NB300-213] - Mouse primary cultures stained with MAP2 antibody. ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: MAP2 Antibody [NB300-213] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to microtubule associated protein 2 (MAP2), NB300-213, dilution 1:10,000 in red, and mouse mAb to MAP-tau, NPB2-25162, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. NB300-213 antibody stains dendrites and perikarya of neurons, while NBP2-25162 antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.

Western Blot: MAP2 Antibody [NB300-213] - Western blot analysis of whole brain tissue lysates using chicken pAb to microtubule associated protein 2 (MAP2), NB300-213, dilution 1:50,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain, [4] adult mouse brain. Strong band observed at ~280 kDa mark corresponds to two major isoforms of MAP2 protein referred to as MAP2A and MAP2B. Smaller fragments of these isoforms are also detected if the antibody is used at higher concentrations.

Immunocytochemistry/Immunofluorescence: MAP2 Antibody [NB300-213] - PEA decreases astrocyte activation in organotypic cultures of rat hippocampi and rescues neuronal CA3 damage caused by Abeta challenge. Abeta-challenged (1 ug/ml) slices of rat hippocampi were treated for 24 hours with PEA (0.1 uM) in the presence of the selective PPAR? antagonist (GW9662, 9 nM) or the selective PPARalpha antagonist (MK886, 3 uM). Representative photomicrographs of the CA3 region showing the results of immunofluorescence experiments aimed at investigating the effect of treatments on astrocyte activation and neuronal loss, as determined by immunostaining for GFAP (green) and MAP2 (red) alone or merged, respectively. Nuclei were stained with Hoechst (blue). Scale bar: 10 um. Arrows in the photomicrographs indicate astrocyte infiltration events and apoptotic condensation in the nuclei of adjacent neurons.

Immunocytochemistry/Immunofluorescence: MAP2 Antibody [NB300-213] - Human iPS derived neurons labeled with MAP2 (in green) at 1:5000 dilution. Image from verified customer review.

Immunohistochemistry-Frozen: MAP2 Antibody [NB300-213] - MAP2 in the retina detected by NB300-213 antibody (magenta). DAPI (blue). Image from verified customer review.

Immunocytochemistry/Immunofluorescence: MAP2 Antibody [NB300-213] - Human DA neurons 28 DIV stained with MAP2 antibody (blue). ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: MAP2 Antibody [NB300-213] - PEA inhibits astroglial proliferation and reduces neuronal loss in mixed neuroglia co-cultures exposed to Abeta. Abeta-challenged (1 ug/ml) astrocyte/neuron mixed cultures were treated with PEA (0.1 uM) in the presence of the selective PPAR-gamma antagonist (GW9662, 9 nM) or the selective PPARalpha antagonist (MK886, 3 uM). After 24 hours of treatment, cells were processed for analyses. Immunofluorescence photomicrographs showing the effect of the treatments on astrocyte proliferation and neuronal loss, as determined by immunostaining for GFAP (green) and MAP2 (red), respectively. Arrows indicate chromatin condensation in nuclei stained with Hoechst dye (blue) as markers of apoptotic events. Scale bar: 10 um. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-9-49), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: MAP2 Antibody [NB300-213] - Oxytocin receptors (Oxtr) are expressed in mouse hippocampus. Confocal images of Oxtr-expressing neurons in the CA1 subfields of the medial and caudal hippocampus from OxtrVn/+ sections, triple stained with antibodies directed against NeuN (red), MAP2 (magenta), and Venus (green). Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/22466), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: MAP2 Antibody [NB300-213] - Differential and neuron-specific Oxtr expression in mouse hippocampus. Immunohistochemical mapping of Venus expression in coronal sections of the rostral, medial, and caudal hippocampus from three weeks old OxtrVn/+ mice triple-labeled with antibodies against NeuN (red), Venus (green), and Map2 (magenta). Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/22466), licensed under a CC-BY licence.

• Reactivity: Hu, Mu, Rt, Fe
• Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, KD
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated

MAP2 Antibody Summary

• Immunogen: This MAP2 antibody was developed against a mix of recombinant human construct of projection domain sequences, amino acids 377-1505: Prot-r-MAP2-P1, Prot-r-MAP2-P2 and Prot-r-MAP2-P3.
• Localization: Cytoskeleton
• Marker: Neuronal Dendritic Marker
• Specificity: This antibody was raised against recombinant constructs of the entire human projection domain, and so recognizes the high molecular MAP2 forms, MAP2A and MAP2B.
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: MAP2
• Purity: IgY purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:5000-1:10000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Frozen 1:1000-1:5000
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot 1:10000-1:20000
• Theoretical MW: 199 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 32848625). Mouse reactivity reported in scientific literature (PMID: 33086056). Feline reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Supplied as concentrated IgY in PBS
• Preservative: 5mM Sodium Azide
• Purity: IgY purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for MAP2 Antibody

• DKFZp686I2148
• MAP2
• MAP-2
• MAP2A
• MAP2B
• MAP2C
• Microtubule Associated Protein 2
• microtubule-associated protein 2
• MTAP2

Background

Microtubule-associated protein 2 also known as MAP2 (theoretical molecular weight 280 kDa) belongs to the MAP2/Tau family which in addition to the two neuronal forms MAP2 and Tau also includes MAP4 (1). For MAP2, four different isoforms have been identified which are produced through alternative splicing including MAP2a, MAP2b, MAP2c and MAP2d (1). MAP2 isoforms contain multiple microtubule-binding domains located near the carboxy terminal, a variably sized amino-terminal projection domain, and a conserved protein kinase A binding domain. MAP2c and MAP2d isoforms have a shorter amino-terminal projection domain, lower molecular weight and are identifiable at ~70 kDa in SDS-PAGE analysis (2,3). MAP2's carboxy terminal domain supports interactions with microtubules, actin, and intermediate filaments (2).

MAP2 isoforms are developmentally regulated and differentially expressed in neurons and some glia. MAP2c is predominantly expressed in the developing brain while the other isoforms are expressed in the adult brain. The distribution of MAP2 isoforms also varies, with MAP2a and MAP2b predominantly localized to dendrites, while MAP2c is also found in axons. Lastly, the expression of MAP2d is not limited to neurons and may be found in glia, specifically oligodendrocytes (1, 2). MAP2 isoforms associate with microtubules and mediate their interaction with actin filaments thereby playing a critical role in organizing the microtubule-actin network. In neurons, MAP2 isoforms are implicated in different processes including neurite initiation, elongation and stabilization as well as axon and dendrite formation (2). Knockout of MAP expression in animal models results in a variety of functional and structural brain defects according to the isoform affected (e.g., reduced LTP and LTD, reduced myelination, absence of corpus collosum, motor system malfunction, abnormal hippocampal dendritic morphology, abnormal synaptic plasticity) (4).

References

1. Dehmelt, L., & Halpain, S. (2005). The MAP2/Tau family of microtubule-associated proteins. Genome Biology. https://doi.org/10.1186/gb-2004-6-1-204

2. Mohan, R., & John, A. (2015). Microtubule-associated proteins as direct crosslinkers of actin filaments and microtubules. IUBMB Life. https://doi.org/10.1002/iub.1384

3. Shafit-Zagardo, B., & Kalcheva, N. (1998). Making sense of the multiple MAP-2 transcripts and their role in the neuron. Molecular Neurobiology. https://doi.org/10.1007/BF02740642

4. Tortosa, E., Kapitein, L. C., & Hoogenraad, C. C. (2016). Microtubule organization and microtubule-associated proteins (MAPs). In Dendrites: Development and Disease. https://doi.org/10.1007/978-4-431-56050-0_3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MAP2 Antibody (NB300-213)(83)

Publications using NB300-213

• Sinha Ray S, Dutta D, Dennys C et al. Mechanisms of IRF2BPL-related disorders and identification of a potential therapeutic strategy Cell reports 2022-12-06 [PMID: 36476864] (ICC/IF, Human)
• Amar F. Cellular And Molecular Mechanism Of Action Of The Amyloid-Beta Oligomer Abeta Star 56. Thesis. 2016-01-01
• Cicardi ME, Hallgren JH, Mawrie D et al. C9orf72 poly(PR) mediated neurodegeneration is associated with nucleolar stress bioRxiv 2023-02-16 [PMID: 36824930] (ICC/IF)
• Ren Y, Huang Z, Zhou L et al. Spatial transcriptomics reveals niche-specific enrichment and vulnerabilities of radial glial stem-like cells in malignant gliomas Nature communications 2023-02-23 [PMID: 36823172] (ICC/IF, Human)
• Griffin H, Sullivan S, Barger S et al. Liraglutide Counteracts Endoplasmic Reticulum Stress in Palmitate-Treated Hypothalamic Neurons without Restoring Mitochondrial Homeostasis International Journal of Molecular Sciences 2022-12-30 [PMID: 36614074] (ICC/IF, Mouse)
• Juntunen M Development of Adipose-Derived Stromal/stem Cell-Based Therapies : Effects of donor weight and culture conditions on cell characteristics and testing the efficacy in stroke models Thesis 2022-01-01 (ICC/IF, Human)
• Buscaglia GC Tuba1a microtubules establish the foundations for neuronal function Thesis 2020-01-01
• Yu H, Lu S, Gasior K et al. TDP-43 and HSP70 phase separate into anisotropic, intranuclear liquid spherical annuli bioRxiv 2020-01-01
• Singh B Investigating the mechanisms underlying synaptic and cognitive deficits in alpha-synucleinopathies Thesis 2019-01-01 (ICC/IF, Mouse)
• Borisova E Studies on the formation of cortical circuits Thesis 2022-01-01 (ICC/IF, Mouse)
• RAsAnen N, Harju V, Joki T, Narkilahti S Practical guide for preparation, computational reconstruction and analysis of 3D human neuronal networks in control and ischaemic conditions Development (Cambridge, England) 2022-10-15 [PMID: 35929583] (ICC/IF, Human)
  
  Details:
  Neurons in hydrogels, dilution used 1:2000
• Shi HJ, Wu DL, Chen R et al. Requirement of hippocampal DG nNOS-CAPON dissociation for the anxiolytic and antidepressant effects of fluoxetine Theranostics 2022-05-01 [PMID: 35664081] (ICC/IF, Mouse)
• Zhao Y, Hu D, Wang R et al. ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimer's disease models Nature communications 2022-03-02 [PMID: 35236834] (ICC/IF, Mouse)
• Harbauer AB, Hees JT, Wanderoy S et al. Neuronal mitochondria transport Pink1 mRNA via synaptojanin 2 to support local mitophagy Neuron 2022-02-19 [PMID: 35216662]
• Mzezewa R, Lotila J, Kiiski H et al. A kainic acid-induced seizure model in human pluripotent stem cell-derived cortical neurons for studying the role of IL-6 in the functional activity Stem cell research 2022-01-17 [PMID: 35091307] (ICC/IF, Human)
• Dickerson MR, Murphy SF, Urban MJ et al. Chronic Anxiety- and Depression-Like Behaviors Are Associated With Glial-Driven Pathology Following Repeated Blast Induced Neurotrauma Frontiers in behavioral neuroscience 2021-12-10 [PMID: 34955781] (IF/IHC, Rat)
• Samanta S, YlA-Outinen L, Rangasami VK et al. Bidirectional cell-matrix interaction dictates neuronal network formation in a brain-mimetic 3D scaffold Acta biomaterialia 2021-12-10 [PMID: 34902615] (ICC/IF, Human)
• Tian Y, Wang Y, Jablonski AM Et al. Tau-tubulin kinase 1 phosphorylates TDP-43 at disease-relevant sites and exacerbates TDP-43 pathology Neurobiology of disease 2021-11-06 [PMID: 34752923] (ICC/IF, Human)
• Lipstein N, Chang S, Lin KH Et al. Munc13-1 is a Ca2+-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission Neuron 2021-10-21 [PMID: 34706220] (ICC/IF, Mouse)
• Pollard KJ, Bowser DA, Anderson WA Et al. Morphine-sensitive synaptic transmission emerges in embryonic rat microphysiological model of lower afferent nociceptive signaling Science advances 2021-08-01 [PMID: 34452921] (IF/IHC, Rat)
• Kang Y, Zhou Y, Li Y Et al. A human forebrain organoid model of fragile X syndrome exhibits altered neurogenesis and highlights new treatment strategies Nature neuroscience 2021-08-19 [PMID: 34413513] (ICC/IF, Human)
• Basu H, Pekkurnaz G, Falk J Et al. FHL2 anchors mitochondria to actin and adapts mitochondrial dynamics to glucose supply The Journal of cell biology 2021-10-04 [PMID: 34342639] (ICC/IF)
• Trinkaus VA, Riera-Tur I, MartInez-SAnchez A et al. In situ architecture of neuronal alpha-Synuclein inclusions Nature communications 2021-04-14 [PMID: 33854052]
• Srikanth MP, Jones JW, Kane M et al. Elevated glucosylsphingosine in Gaucher disease induced pluripotent stem cell neurons deregulates lysosomal compartment through mammalian target of rapamycin complex 1 Stem cells translational medicine 2021-03-03 [PMID: 33656802] (ICC/IF, Human)
• Jablonski AM, Warren L, Usenovic M et al. Astrocytic expression of the Alzheimer's disease risk allele, ApoE epsilon 4, potentiates neuronal tau pathology in multiple preclinical models Scientific reports 2021-02-09 [PMID: 33564035] (ICC/IF, Rat)
• Chiola S, Napan KL, Wang Y et al. Defective AMPA-mediated synaptic transmission and morphology in human neurons with hemizygous SHANK3 deletion engrafted in mouse prefrontal cortex Molecular psychiatry 2021-02-08 [PMID: 33558651] (IF/IHC, Mouse)
• Juntunen M, Hagman S, Moisan A et al. In Vitro Oxygen-Glucose Deprivation-Induced Stroke Models with Human Neuroblastoma Cell- and Induced Pluripotent Stem Cell-Derived Neurons Stem Cells International 2020-10-29 [PMID: 33178286] (ICC/IF, Human)
• Lee YL, Kung FC, Lin CH, Huang YS CMTR1-Catalyzed 2'-O-Ribose Methylation Controls Neuronal Development by Regulating Camk2 alpha Expression Independent of RIG-I Signaling Cell Rep 2020-10-20 [PMID: 33086056] (Mouse)
• Pelkonen A, Mzezewa R, Sukki L et al. A modular brain-on-a-chip for modelling epileptic seizures with functionally connected human neuronal networks Biosens Bioelectron 2020-01-01 [PMID: 32877779] (ICC/IF)
• Sun H, Zhang H, Ross A et al. Developmentally Regulated Rebound Depolarization Enhances Spike Timing Precision in Auditory Midbrain Neurons Front Cell Neurosci 2020-08-06 [PMID: 32848625] (IF/IHC, Rat)
• Teodorof-Diedrich C, Spector SA Human Immunodeficiency Virus Type-1 and Methamphetamine Mediated Mitochondrial Damage and Neuronal Degeneration in Human Neurons J. Virol. 2020-08-12 [PMID: 32796068]
• Inglis G, Zhou Y, Patterson D et al. Transcriptomic and epigenomic dynamics associated with development of human iPSC-derived GABAergic interneurons Human Molecular Genetics 2020-07-16 [PMID: 32794569] (ICC/IF, WB, Human)
• Szczurkowska J, Lee SI, Guo A et al. A Localized Scaffold for cGMP Increase Is Required for Apical Dendrite Development Cell Rep 2020-04-14 [PMID: 32294442] (ICC/IF, KD, Human)
• Ripamonti S, Shomroni O, Rhee JS et al. SUMOylation controls the neurodevelopmental function of the transcription factor Zbtb20 J. Neurochem. 2020-03-31 [PMID: 32233089] (ICC/IF, Human)
• Maus L, Lee C, Altas B et Al. Ultrastructural Correlates of Presynaptic Functional Heterogeneity in Hippocampal Synapses Cell Rep 2020-03-17 [PMID: 32187536] (IF/IHC, Mouse)
• Kim BW, Jeong YE, Wong M, Martin LJ DNA damage accumulates and responses are engaged in human ALS brain and spinal motor neurons and DNA repair is activatable in iPSC-derived motor neurons with SOD1 mutations Acta Neuropathol Commun 2020-01-31 [PMID: 32005289] (WB, Human)
• Toh MF, Brooks JM, Strassmaier T et al. Application of High-Throughput Automated Patch-Clamp Electrophysiology to Study Voltage-Gated Ion Channel Function in Primary Cortical Cultures SLAS Discov 2020-01-31 [PMID: 32003306] (WB)
• Bonazzi S, Goold CP, Gray A et al. Discovery of a Brain-Penetrant ATP-Competitive Inhibitor of the Mechanistic Target of Rapamycin (mTOR) for CNS Disorders J. Med. Chem. 2020-02-13 [PMID: 31955578]
• HyvArinen T, Hyysalo A, Kapucu FE et al. Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures Sci Rep 2019-11-20 [PMID: 31748598] (ICC/IF, Rat)
• Sen T, Saha P, Gupta R, et al. Aberrant ER-stress induced neuronal-IFN beta elicits white matter injury due to microglial activation and T-cell infiltration after TBI. J Neurosci. [PMID: 31694961] (IF, Mouse)
• Subramanian J, Michel K, Benoit M, Nedivi E CPG15/Neuritin Mimics Experience in Selecting Excitatory Synapses for Stabilization by Facilitating PSD95 Recruitment Cell Rep 2019-08-06 [PMID: 31390571] (ICC/IF, Mouse, Human)
• Cuesta Torres LF, Zhu W, Ohrling G et al. An Autaptic Culture System for Standardized Analyses of iPSC-Derived Human Neurons Cell Rep 2019-05-14 [PMID: 31091457] (ICC/IF, Mouse, Rat)
• Gasset-Rosa F, Lu S, Yu H et al. Cytoplasmic TDP-43 De-mixing Independent of Stress Granules Drives Inhibition of Nuclear Import, Loss of Nuclear TDP-43, and Cell Death Neuron 2019-03-05 [PMID: 30853299] (ICC/IF, Human)
• Kobayakawa K, DePetro KA, Zhong H et al. Locomotor Training Increases Synaptic Structure With High NGL-2 Expression After Spinal Cord Hemisection Neurorehabil Neural Repair 2019-02-19 [PMID: 30782076] (IHC-Fr, Rat)
• Bathini P, Mottas A, Jaquet M et al. Progressive signaling changes in the olfactory nerve of patients with Alzheimer's disease Neurobiol. Aging 2019-04-01 [PMID: 30708185] (ICC/IF, Human)
• Aiken J, Moore J, Bates E. TUBA1A mutations identified in lissencephaly patients dominantly disrupt neuronal migration and impair dynein activity. bioRxiv 2018-07-25 [PMID: 30517687] (ICC/IF, Rat)
• Ambrozkiewicz MC, Schwark M, Kishimoto-Suga M et al. Polarity Acquisition in Cortical Neurons Is Driven by Synergistic Action of Sox9-Regulated Wwp1 and Wwp2 E3 Ubiquitin Ligases and Intronic miR-140. Neuron. 2018-10-30 [PMID: 30392800] (ICC/IF, Mouse)
• Lopez-Erauskin J, Tadokoro T, Baughn MW et al. ALS/FTD-Linked Mutation in FUS Suppresses Intra-axonal Protein Synthesis and Drives Disease Without Nuclear Loss-of-Function of FUS. Neuron. 2018-10-17 [PMID: 30344044] (Mouse)
• khan SS, LaCroix M, BoYle G et al. Bidirectional modulation of Alzheimer phenotype by alpha-synuclein in mice and primary neurons. Acta Neuropathol. 2018-07-11 [PMID: 29995210] (ICC/IF, Mouse)
• Zhu LJ, Ni HY, Chen R et al. Hippocampal NF-kB accounts for stress-induced anxiety behaviors via enhancing nNOS-CAPON-Dexras1 coupling J. Neurochem. 2018-06-02 [PMID: 29858554] (Mouse)
• Stankova T, Piepkorn L, Bayer TA et al. SUMO1-conjugation is altered during normal aging but not by increased amyloid burden. Aging Cell 2018-04-06 [PMID: 29633471] (IF/IHC, Mouse)
• Hsu F, Spannl S, Ferguson C et al. Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria. bioRxiv. [PMID: 29469808] (ICC/IF, Human)
• Paavilainen T, Pelkonen A, Makinen ME, Peltola M. Effect of prolonged differentiation on functional maturation of human pluripotent stem cell-derived neuronal cultures. Stem Cell Res. 2018-01-29 [PMID: 29414606] (Human)
• Daniel JA, Cooper BH, Palvimo JJ et al. Analysis of SUMO1-conjugation at synapses Elife 2017-06-09 [PMID: 28598330] (ICC/IF, Mouse)
• Amar F, Sherman MA, Rush T et al. The amyloid-B oligomer AB*56 induces specific alterations in neuronal signaling that lead to tau phosphorylation and aggregation. Sci Signal. 2017-05-09 [PMID: 28487416] (Human)
• Zhao J, Davis MD, Martens YA et al. APOE E4/E4 diminishes neurotrophic function of human iPSC-derived astrocytes. Hum. Mol. Genet. 2017-07-15 [PMID: 28444230] (ICC/IF, Human)
• Ripamonti S, Ambrozkiewicz MC, Guzzi F et al. Transient oxytocin signaling primes the development and function of excitatory hippocampal neurons. Elife. 2017-02-23 [PMID: 28231043] (ICC/IF, Mouse)
• Lagerlof O, Hart GW, Huganir RL. O-GlcNAc transferase regulates excitatory synapse maturity Proc. Natl. Acad. Sci. U.S.A 2017-01-31 [PMID: 28143929] (IF/IHC, Mouse)
• Sherman M A, LaCroix M et al. Soluble Conformers of A beta and Tau Alter Selective Proteins Governing Axonal Transport. J Neurosci 2016-09-14 [PMID: 27629715] (WB, Mouse)
• Ciaraldi TP, Ryan AJ, Mudaliar SR, Henry RR Altered Myokine Secretion Is an Intrinsic Property of Skeletal Muscle in Type 2 Diabetes. PLoS One 2016-07-25 [PMID: 27453994] (ICC/IF, Human)
• Verma N, Ly H, Liu M et al. Intraneuronal Amylin Deposition Peroxidative Membrane Injury and Increased IL-1beta Synthesis in Brains of Alzheimer's Disease Patients with Type-2 Diabetes and in Diabetic HIP Rats. J. Alzheimers Dis. 2016-05-05 [PMID: 27163815] (ICC/IF, Rat)
• Haas LT, Salazar SV, Kostylev MA et al. Metabotropic glutamate receptor 5 couples cellular prion protein to intracellular signalling in Alzheimer's disease. Brain. 2015-12-14 [PMID: 26667279] (ICC/IF, Mouse)
• Zhou H, Liu G. Regulation of density of functional presynaptic terminals by local energy supply. Mol Brain 2015-01-01 [PMID: 26184109] (ICC/IF)
• Usui Y, Westenskow PD, Kurihara T et al. Neurovascular crosstalk between interneurons and capillaries is required for vision. J. Clin. Invest. 2015-06-01 [PMID: 25915585] (Mouse)
  
  Details:
  MAP2 antibody was used in IHC application for immunostaining of microtubule-associated protein 2 (a marker for neuronal dendrites) on whole-mount retinas at P23 of mice ( P23 Ptf1a-Cre R26^tdTomato/+ retinas). This staining depicted the colocalization of amacrine and horizontal cell neurites with the intraretinal vasculature (Figure 1 - H and -I).
• Schwerk A, Altschuler J, Roch M et al. Human adipose-derived mesenchymal stromal cells increase endogenous neurogenesis in the rat subventricular zone acutely after 6-hydroxydopamine lesioning. Cytotherapy. 2014-10-24 [PMID: 25457280] (IHC-Fr, Human)
  
  Details:
  MAP2/MAP2b antibody used as neuronal marker in IHC-Fr immunostaining the human adipose-derived mesenchymal stromal cells transplanted into the rat substantia nigra in the 6-hydroxydopamine model of Parkinson's disease
• Oenarto J, Gorg B, Moos M et al. Expression of organic osmolyte transporters in cultured rat astrocytes and rat and human cerebral cortex. Arch. Biochem. Biophys. 2014-07-05 [PMID: 25004465] (IHC-Fr, ICC/IF, Rat, Human)
  
  Details:
  MAP2 antibody used at 1:200 dilution for ICC-IF on cultured rat brain cells (Fig 2B) and for IHC-Fr on Rat / Human brain sections (Fig 4).
• Khacho M, Tarabay M, Patten D et al. Acidosis overrides oxygen deprivation to maintain mitochondrial function and cell survival. Nat Commun 2014-04-01 [PMID: 24686499] (ICC/IF, Mouse)
• Nieland Thomas J F, Logan David J, Saulnier Jessica et al. High content image analysis identifies novel regulators of synaptogenesis in a high-throughput RNAi screen of primary neurons. PLoS One. 2014-03-14 [PMID: 24633176] (ICC/IF, Mouse)
• Sharma K, Choi SY, Zhang Y et al. High-Throughput Genetic Screen for Synaptogenic Factors: Identification of LRP6 as Critical for Excitatory Synapse Development. Cell Rep 2013-12-12 [PMID: 24316074] (ICC/IF, Mouse)
• Liang CL, Marks GA, et al. GABAA receptors are located in cholinergic terminals in the nucleus pontis oralis of the rat: implications for REM sleep control. Brain Res. 2013-10-17 [PMID: 24141149] (ICC/IF, IF/IHC, Rat)
• Abdullah H, Heaney LG, Cosby SL, McGarvey LP. Rhinovirus upregulates transient receptor potential channels in a human neuronal cell line: implications for respiratory virus-induced cough reflex sensitivity. Thorax. 2013-09-03 [PMID: 24002057]
• Grant P, Ahlemeyer B, Karnati S et al. The biogenesis protein PEX14 is an optimal marker for the identification and localization of peroxisomes in different cell types, tissues, and species in morphological studies. Histochem Cell Biol. 2013-08-20 [PMID: 23959168] (ICC/IF)
• Chen Z, Cooper B, Kalla S et al. The Munc13 Proteins Differentially Regulate Readily Releasable Pool Dynamics and Calcium-Dependent Recovery at a Central Synapse. J Neurosci 2013-05-08 [PMID: 23658173] (ICC/IF, IHC-Fr, Mouse)
• Nair R, Lauks J, Jung S et al. Neurobeachin regulates neurotransmitter receptor trafficking to synapses J Cell Biol 2012-12-31 [PMID: 23277425] (IF/IHC, ICC/IF, Mouse)
• Larson M, Sherman MA, Amar F et al. The Complex PrPc-Fyn Couples Human Oligomeric Abeta with Pathological Tau Changes in Alzheimer's Disease J Neurosci 2012-11-21 [PMID: 23175838] (ICC/IF, IF/IHC, Human)
• Colgan LA, Cavolo SL, Commons KG, Levitan ES. Action Potential-Independent and Pharmacologically Unique Vesicular Serotonin Release from Dendrites J Neurosci 2012-11-07 [PMID: 23136413] (ICC/IF, IF/IHC, Rat)
• Chao HW, Lai YT, Lu YL et al. NMDAR signaling facilitates the IPO5-mediated nuclear import of CPEB3 Nucleic Acids Res 2012-06-22 [PMID: 22730302] (ICC/IF, Mouse, Rat)
• Scuderi C, Valenza M, Stecca C et al. Palmitoylethanolamide exerts neuroprotective effects in mixed neuroglial cultures and organotypic hippocampal slices via peroxisome proliferator-activated receptor-alpha J Neuroinflammation 2012-03-09 [PMID: 22405189] (ICC/IF, Rat)
• Schatzle P, Wuttke R, Ziegler U, Sonderegger P. Automated quantification of synapses by fluorescence microscopy. Journal of Neuroscience Methods 11204(1):144-149. 2011-11-01 [PMID: 22108140] (ICC/IF, Mouse)
• Sur S, Pashuck ET, Guler MO et al. A hybrid nanofiber matrix to control the survival and maturation of brain neurons. Biomaterials; 22018390]. 2011-10-19 [PMID: 22018390] (ICC/IF, Rat)
• Li Y, Serwanski DR, Miralles CP et al. Synaptic and nonsynaptic localization of protocadherin-gammaC5 in the rat brain. J Comp Neurol;518(17):3439-63. 2010-09-01 [PMID: 20589908] (ICC/IF, Human)

Reviews for MAP2 Antibody (NB300-213) (7)

Average Rating: 5

(Based on 7 reviews)

We have 7 reviews tested in 4 species: Human, Mouse, Rat, Feline.

reviewed by: Verified Customer ICC Human 12/08/2022

Summary

• Application: Immunocytochemistry
• Sample Tested: human iPS cortical neurons
• Species: Human
• Lot: 7377-102022

Comments

• Comments: Human iPS derived neurons labeled with MAP2 (in green) at 1:5000 dilution.

reviewed by: David Silva IHC-Fr Feline 11/19/2022

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Retina
• Species: Feline
• Lot: 7377-102022

Comments

• Comments: ***Novus Innovators Program - new species or application used on a primary antibody.***

reviewed by: Tatyana Martynova IHC-Fr Mouse 07/29/2021

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Astrocytes
• Species: Mouse
• Lot: D1

reviewed by: Verified Customer ICC Rat 11/01/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Cultured motor neurons
• Species: Rat
• Lot: 7225-9

Comments

• Comments: Used at a dilution of 1:2500. incubated at 4 degrees overnight. Robust staining

reviewed by: Verified Customer ICC Mouse 11/22/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse primary cultures
• Species: Mouse

Comments

• Comments: 1:1000 dilution

reviewed by: BEN CHIH IF Human 01/22/2016

Summary

• Application: Immunofluorescence
• Sample Tested: Rat and Human.
• Species: Human

reviewed by: Verified Customer IHC-Fr Rat 12/07/2010

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Rat brain tissue
• Species: Rat

FAQs for MAP2 Antibody (NB300-213). (Showing 1 - 3 of 3 FAQs).

1. Can you please tell me the country of origin for antibody NB300-213, chicken anti-MAP2?
   • Our product NB300-213 originates in the United States.
2. Would you be able to share the concentration of NB300-213 from Lot# 7214, and the current lot of this antibody?
   • This antibody is a chicken polyclonal that has been precipitated therefore a concentration is irrelevant. This is because, unlike an affinity purified antibody, there will be other proteins that absorb at 280 that are not the specific antibody to the protein target. The concentration is usually about 20mg/ml. We currently are on Lot# 7225-3.
3. I was using your product NB300-213 for MAP2 immunofluorescence, I did many times, which showed a beaded signal consistently unlike the picture presented on the product datasheet,could you give me some advice?
   • I looked at the images, and I think that the MAP2 antibody is working fine. The beading of the dendrites is likely due to some problem with fixation, which resulted in some damage to the neurons. MAP2 is bound to microtubules which are very labile, rapidly breaking down in the cold or if the cells are metabolically or mechanically challenged. When this happens, the dendrites take up a beaded appearance, as in the images. We routinely use a fixation protocol in which cells are fixed briefly in formalin at room temperature, which stabilizes the microtubules, followed by cold methanol, which helps to permeabilize the cells.

Bioinformatics

• Gene Symbol: MAP2
• Entrez:
  • 4133(Human)
  • 17756(Mouse)
  • 25595(Rat)
• Uniprot:
  • P11137()