MAP2 Antibody

Images

 
Western Blot: MAP2 Antibody [NB300-213] - Western blot analysis of MAP2 expression in bovine brain extract using NB300-213.
Immunocytochemistry/ Immunofluorescence: MAP2 Antibody [NB300-213] - Mouse primary cultures stained with MAP2 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: MAP2 Antibody [NB300-213] - Mixed neuron/glial cultures stained with NB300-213 (red). The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 ...read more
Immunofluorescence: MAP2 Antibody [NB300-213] - Human DA neurons 28 DIV stained with MAP2 antibody (blue). Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated

Order Details

MAP2 Antibody Summary

Immunogen
Purified MAP2 isolated from bovine brain.
Marker
Neuronal Dendritic Marker
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
MAP2
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000-1:10000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Frozen 1:1000-1:5000
Application Notes
This MAP2 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen sections and Western blot. In Western blot bands can be seen at approximately 280 kDa, representing MAP2a and MAP2b. Bands may also be seen at approximately 70 kDa, representing MAP2c. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
280 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 4 Reviews rated 5
using
NB300-213 in the following applications:

Publications
Read Publications using
NB300-213 in the following applications:

Reactivity Notes

Human, mouse, rat and bovine. Other species have not been tested.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
5mM Sodium Azide
Purity
Ammonium sulfate precipitation

Alternate Names for MAP2 Antibody

  • DKFZp686I2148
  • MAP2
  • MAP-2
  • MAP2A
  • MAP2B
  • MAP2C
  • microtubule-associated protein 2

Background

MAP2 belongs to a family of proteins known as the microtubule associated proteins (MAPs). MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. Antibodies to MAP2 are excellent markers on neuronal cells, their perikarya and neuronal dendrites. Dendrite specific MAP is found specifically in the dendritic branching of neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MAP2 Antibody (NB300-213)(26)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-Fr.


Filter By Application
ICC/IF
(21)
IHC
(5)
IHC-Fr
(3)
All Applications
Filter By Species
Human
(5)
Mouse
(12)
Rat
(7)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using NB300-213 Applications Species
Daniel JA, Cooper BH, Palvimo JJ et al. Analysis of SUMO1-conjugation at synapses Elife 2017 Jun 09 [PMID: 28598330] (ICC/IF, Mouse) ICC/IF Mouse
Ripamonti S, Ambrozkiewicz MC, Guzzi F et al. Transient oxytocin signaling primes the development and function of excitatory hippocampal neurons. Elife. 2017 Feb 23 [PMID: 28231043] (ICC/IF, Mouse) ICC/IF Mouse
Lagerlof O, Hart GW, Huganir RL. O-GlcNAc transferase regulates excitatory synapse maturity Proc. Natl. Acad. Sci. U.S.A Jan 31 2017 [PMID: 28143929] (IHC, Mouse) IHC Mouse
Ciaraldi TP, Ryan AJ, Mudaliar SR, Henry RR. Altered Myokine Secretion Is an Intrinsic Property of Skeletal Muscle in Type 2 Diabetes PLoS ONE 2016 Jul 26 [PMID: 27453994] (ICC/IF, Human) ICC/IF Human
Verma N, Ly H, Liu M et al. Intraneuronal Amylin Deposition Peroxidative Membrane Injury and Increased IL-1beta Synthesis in Brains of Alzheimer's Disease Patients with Type-2 Diabetes and in Diabetic HIP Rats. J. Alzheimers Dis. May 5 2016 [PMID: 27163815] (ICC/IF, Rat) ICC/IF Rat
Haas LT, Salazar SV, Kostylev MA et al. Metabotropic glutamate receptor 5 couples cellular prion protein to intracellular signalling in Alzheimer's disease. Brain. 2015 Dec 14 [PMID: 26667279] (ICC/IF, Mouse) ICC/IF Mouse
Zhou H, Liu G. Regulation of density of functional presynaptic terminals by local energy supply. Mol Brain 2015 [PMID: 26184109] (ICC/IF) ICC/IF
Usui Y, Westenskow PD, Kurihara T et al. Neurovascular crosstalk between interneurons and capillaries is required for vision. J. Clin. Invest. 2015 Jun 01 [PMID: 25915585] (Mouse)

Details:
MAP2 antibody was used in IHC application for immunostaining of microtubule-associated protein 2 (a marker for neuronal dendrites) on whole-mount retinas at P23 of mice ( P23 Ptf1a-Cre R26^tdTomato/+ retinas). This staining depicted the colocalization of amacrine and horizontal cell neurites with the intraretinal vasculature (Figure 1 - H and -I).
Mouse
Schwerk A, Altschuler J, Roch M et al. Human adipose-derived mesenchymal stromal cells increase endogenous neurogenesis in the rat subventricular zone acutely after 6-hydroxydopamine lesioning. Cytotherapy. 2014 Oct 24 [PMID: 25457280] (IHC-Fr, Human)

Details:
MAP2/MAP2b antibody used as neuronal marker in IHC-Fr immunostaining the human adipose-derived mesenchymal stromal cells transplanted into the rat substantia nigra in the 6-hydroxydopamine model of Parkinson's disease
IHC-Fr Human
Oenarto J, Gorg B, Moos M et al. Expression of organic osmolyte transporters in cultured rat astrocytes and rat and human cerebral cortex. Arch. Biochem. Biophys. 2014 Jul 05 [PMID: 25004465] (ICC/IF, IHC-Fr, Rat, Human)

Details:
MAP2 antibody used at 1:200 dilution for ICC-IF on cultured rat brain cells (Fig 2B) and for IHC-Fr on Rat / Human brain sections (Fig 4).
ICC/IF, IHC-Fr Rat, Human
Show All 26 Publications.

Reviews for MAP2 Antibody (NB300-213) (4) 54

Average Rating: 5
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse, Rat.
We have 4 reviews tested in 3 application: ICC, IF, IHC-Fr.

Reviews using NB300-213:
Filter by Applications
ICC
(2)
IF
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Rat
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry MAP2 NB300-213
Enlarge
reviewed by:
Karthik Krishnamurthy
ICC Rat 11/01/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedCultured motor neurons
SpeciesRat
Lot7225-9

Comments

CommentsUsed at a dilution of 1:2500. incubated at 4 degrees overnight. Robust staining
Immunocytochemistry MAP2 NB300-213
Enlarge
reviewed by:
Lingling Yang
ICC Mouse 11/22/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse primary cultures
SpeciesMouse

Comments

Comments1:1000 dilution
Immunofluorescence MAP2 NB300-213
Enlarge
reviewed by:
Anonymous
IF Human 01/22/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedRat and Human.
SpeciesHuman

Blocking

Blocking Details2%BSA, 2% Donekey serum, 0.1% Triton X100

Primary Anitbody

Dilution Ratio1:2000 in block

Secondary Antibody

Secondary DescriptionDonkey anti Chick-Alexa 647
Secondary Manufacturer Cat#JacksonImmuno
Secondary Concentration1:800

Details

Detection NotesImaged on Leica SP5 confocal microscope
Fixation DetailsPFA/MetOH fixed.
Wash DescriptionPBS 3X
 
reviewed by:
Foster Olive
IHC-Fr Rat 12/07/2010
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRat brain tissue
SpeciesRat
CommentsGreat staining patterns!

Blocking

Blocking DetailsBlocking Buffer: 5% donkey serum, Blocking Time: 2 hours, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1:1000, Incubation Dilution Buffer: PBS + 0.1% Tween + 5% donkey serum, Incubation Time: overnight, Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Dylight488 donkey anti-rabbit, Secondary Ab Dilution: 1:500

Details

Detection NotesDetection Method: epifluorescence
Fixation DetailsSample Preparation: OCT embedded Cryosection

Comments

CommentsGreat staining patterns!

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MAP2 Antibody (NB300-213). (Showing 1 - 1 of 1 FAQs).

  1. Can you please tell me the country of origin for antibody NB300-213, chicken anti-MAP2?
    • Our product NB300-213 originates in the United States.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for MAP2 Antibody (NB300-213)

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Blogs on MAP2.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

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Recent Reviews

5
5
4
4
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3
0
2
0
1
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Karthik Krishnamurthy
11/01/2017
Application: ICC
Species: Rat

Lingling Yang
11/22/2016
Application: ICC
Species: Mouse

Anonymous
01/22/2016
Application: IF
Species: Human

Bioinformatics

Gene Symbol MAP2
Entrez
Uniprot