MAP2 Antibody

Images

 
Mouse primary cultures stained with MAP2 antibody. ICC/IF image submitted by a verified customer review.
Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to microtubule associated protein 2 (MAP2), NB300-213, dilution 1:10,000 in red, and mouse mAb to MAP-tau, ...read more
Western blot analysis of whole brain tissue lysates using chicken pAb to microtubule associated protein 2 (MAP2), NB300-213, dilution 1:50,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 ...read more
PEA decreases astrocyte activation in organotypic cultures of rat hippocampi and rescues neuronal CA3 damage caused by Abeta challenge. Abeta-challenged (1 ug/ml) slices of rat hippocampi were treated for 24 hours with ...read more
Human iPS derived neurons labeled with MAP2 (in green) at 1:5000 dilution. Image from verified customer review.
MAP2 in the retina detected by NB300-213 antibody (magenta). DAPI (blue). Image from verified customer review.
Human DA neurons 28 DIV stained with MAP2 antibody (blue). ICC/IF image submitted by a verified customer review.
PEA inhibits astroglial proliferation and reduces neuronal loss in mixed neuroglia co-cultures exposed to Abeta. Abeta-challenged (1 ug/ml) astrocyte/neuron mixed cultures were treated with PEA (0.1 uM) i9662, 9 nM) on ...read more
Oxytocin receptors (Oxtr) are expressed in mouse hippocampus. Confocal images of Oxtr-expressing neurons in the CA1 subfields of the medial and caudal hippocampus from OxtrVn/+ sections, triple stained with antibodies ...read more
Differential and neuron-specific Oxtr expression in mouse hippocampus. Immunohistochemical mapping of Venus expression in coronal sections of the rostral, medial, and caudal hippocampus from three weeks old OxtrVn/+ ...read more
Tuba1aND neurons do not correctly localize Map1b to the developing growth cone. (E) Representative images showing altered subcellular distribution of Map1b in Tuba1aND/+ (bottom) cortical neurons compared to WT (top) at ...read more
GPR50 competes with FKBP12 for the binding to T beta RI. a (Left) HEK293T cells were transfected with Flag-T beta RI alone or cotransfected with HA-T beta RII (with TGF beta ; 2 ng/mL; 1 h), myc-FKBP12 and either ...read more
ATM activation by chloroquine alleviates senescence.(a) Immunoblots showing protein levels of ATM, NBS1, and RAP80 in human skin fibroblasts (HSFs). A gradually increased level of p16 indicates cellular ...read more
Distribution of gamma H2AX and 53BP1 foci induced by CDDP in IHCs versus OHCsAConfocal images showing the basal region of organ of Corti cultures treated with either culture medium alone or medium containing 10 μM ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, FeSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated

Order Details

MAP2 Antibody Summary

Immunogen
This MAP2 antibody was developed against a mix of recombinant human construct of projection domain sequences, amino acids 377-1505: Prot-r-MAP2-P1, Prot-r-MAP2-P2 and Prot-r-MAP2-P3.
Localization
Cytoskeleton
Marker
Neuronal Dendritic Marker
Specificity
This antibody was raised against recombinant constructs of the entire human projection domain, and so recognizes the high molecular MAP2 forms, MAP2A and MAP2B.
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
MAP2
Purity
IgY purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:5000-1:10000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Frozen 1:1000-1:5000
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot 1:10000-1:20000
Theoretical MW
199 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 7 Reviews rated 5
using
NB300-213 in the following applications:

Publications
Read Publications using
NB300-213 in the following applications:

  • 68 publications
  • IF
    1 publication
  • 11 publications
  • IHC
    2 publications
  • 5 publications
  • KD
    1 publication
  • PLA
    1 publication
  • WB
    6 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 32848625). Mouse reactivity reported in scientific literature (PMID: 33086056). Feline reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Supplied as concentrated IgY in PBS
Preservative
0.035% Sodium Azide
Purity
IgY purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for MAP2 Antibody

  • DKFZp686I2148
  • MAP2
  • MAP-2
  • MAP2A
  • MAP2B
  • MAP2C
  • Microtubule Associated Protein 2
  • microtubule-associated protein 2
  • MTAP2

Background

Microtubule-associated protein 2 also known as MAP2 (theoretical molecular weight 280 kDa) belongs to the MAP2/Tau family which in addition to the two neuronal forms MAP2 and Tau also includes MAP4 (1). For MAP2, four different isoforms have been identified which are produced through alternative splicing including MAP2a, MAP2b, MAP2c and MAP2d (1). MAP2 isoforms contain multiple microtubule-binding domains located near the carboxy terminal, a variably sized amino-terminal projection domain, and a conserved protein kinase A binding domain. MAP2c and MAP2d isoforms have a shorter amino-terminal projection domain, lower molecular weight and are identifiable at ~70 kDa in SDS-PAGE analysis (2,3). MAP2's carboxy terminal domain supports interactions with microtubules, actin, and intermediate filaments (2).

MAP2 isoforms are developmentally regulated and differentially expressed in neurons and some glia. MAP2c is predominantly expressed in the developing brain while the other isoforms are expressed in the adult brain. The distribution of MAP2 isoforms also varies, with MAP2a and MAP2b predominantly localized to dendrites, while MAP2c is also found in axons. Lastly, the expression of MAP2d is not limited to neurons and may be found in glia, specifically oligodendrocytes (1, 2). MAP2 isoforms associate with microtubules and mediate their interaction with actin filaments thereby playing a critical role in organizing the microtubule-actin network. In neurons, MAP2 isoforms are implicated in different processes including neurite initiation, elongation and stabilization as well as axon and dendrite formation (2). Knockout of MAP expression in animal models results in a variety of functional and structural brain defects according to the isoform affected (e.g., reduced LTP and LTD, reduced myelination, absence of corpus collosum, motor system malfunction, abnormal hippocampal dendritic morphology, abnormal synaptic plasticity) (4).

References

1. Dehmelt, L., & Halpain, S. (2005). The MAP2/Tau family of microtubule-associated proteins. Genome Biology. https://doi.org/10.1186/gb-2004-6-1-204

2. Mohan, R., & John, A. (2015). Microtubule-associated proteins as direct crosslinkers of actin filaments and microtubules. IUBMB Life. https://doi.org/10.1002/iub.1384

3. Shafit-Zagardo, B., & Kalcheva, N. (1998). Making sense of the multiple MAP-2 transcripts and their role in the neuron. Molecular Neurobiology. https://doi.org/10.1007/BF02740642

4. Tortosa, E., Kapitein, L. C., & Hoogenraad, C. C. (2016). Microtubule organization and microtubule-associated proteins (MAPs). In Dendrites: Development and Disease. https://doi.org/10.1007/978-4-431-56050-0_3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MAP2 Antibody (NB300-213)(109)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: ICC/IF, IF, IF/IHC, IHC, IHC-Fr, KD, PLA, WB.


Filter By Application
ICC/IF
(68)
IF
(1)
IF/IHC
(11)
IHC
(2)
IHC-Fr
(5)
KD
(1)
PLA
(1)
WB
(6)
All Applications
Filter By Species
Human
(32)
Mouse
(33)
Rat
(18)
All Species
Showing Publications 1 - 10 of 109. Show All 109 Publications.
Publications using NB300-213 Applications Species
Daniel JA, Elizarova S, Shaib AH et al. An intellectual-disability-associated mutation of the transcriptional regulator NACC1 impairs glutamatergic neurotransmission Frontiers in Molecular Neuroscience 2023-07-14 [PMID: 37533751]
Sinha Ray S, Dutta D, Dennys C et al. Mechanisms of IRF2BPL-related disorders and identification of a potential therapeutic strategy Cell reports 2022-12-06 [PMID: 36476864] (ICC/IF, Human) ICC/IF Human
Thibaudeau A, Schmitt K, François L et al. Pharmacological modulation of developmental and synaptic phenotypes in human SHANK3 deficient stem cell-derived neuronal models bioRxiv 2023-09-13 (ICC/IF, Human) ICC/IF Human
Escarcega RD, Patil AA, Moruno-Manchon JF et al. Pirh2-dependent DNA damage in neurons induced by the G-quadruplex ligand pyridostatin Journal of Biological Chemistry 2023-08-12 [PMID: 37579947]
Loedige I, Baranovskii A, Mendonsa S et al. mRNA stability and m6A are major determinants of subcellular mRNA localization in neurons Molecular cell 2023-07-12 [PMID: 37451262] (ICC/IF, Mouse) ICC/IF Mouse
Bonazzi S, Gray A, Thomsen NM et al. Identification of Brain-Penetrant ATP-Competitive mTOR Inhibitors for CNS Syndromes Journal of medicinal chemistry 2023-07-13 [PMID: 37399505]
Kambali M, Li Y, Unichenko P et al. A marker chromosome in psychosis identifies glycine decarboxylase (GLDC) as a novel regulator of neuronal and synaptic function in the hippocampus bioRxiv : the preprint server for biology 2023-05-29 [PMID: 37398055]
Sierra-Delgado JA, Sinha-Ray S, Kaleem A et al. In Vitro Modeling as a Tool for Testing Therapeutics for Spinal Muscular Atrophy and IGHMBP2-Related Disorders Biology 2023-06-16 [PMID: 37372153] (ICC/IF, Human) ICC/IF Human
Nelson AT, Cicardi ME, Markandaiah SS et al. Glucose Hypometabolism Prompts RAN Translation and Exacerbates C9orf72-related ALS/FTD Phenotypes bioRxiv : the preprint server for biology 2023-06-07 [PMID: 37333144] (ICC/IF, Human)

Details:
1:500 dilution
ICC/IF Human
Isosaari L, Vuorenpää H, Yrjänäinen A et al. Simultaneous induction of vasculature and neuronal network formation on a chip reveals a dynamic interrelationship between cell types Cell communication and signaling : CCS 2023-06-14 [PMID: 37316873] (ICC/IF, Human)

Details:
1:200 dilution
ICC/IF Human
Show All 109 Publications.

Reviews for MAP2 Antibody (NB300-213) (7) 57

Average Rating: 5
(Based on 7 reviews)
We have 7 reviews tested in 4 species: Human, Mouse, Rat, Feline.

Reviews using NB300-213:
Filter by Applications
ICC
(3)
IHC-Fr
(3)
IF
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
Rat
(2)
Feline
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry MAP2 NB300-213
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5
reviewed by:
Shashirekha Markandaiah
ICC Human 12/08/2022
View

Summary

ApplicationImmunocytochemistry
Sample Testedhuman iPS cortical neurons
SpeciesHuman
Lot7377-102022

Comments

CommentsHuman iPS derived neurons labeled with MAP2 (in green) at 1:5000 dilution.
Immunohistochemistry-Frozen MAP2 NB300-213
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Feline 11/19/2022
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRetina
SpeciesFeline
Lot7377-102022

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.***
Immunohistochemistry-Frozen MAP2 NB300-213
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Mouse 07/29/2021
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedAstrocytes
SpeciesMouse
LotD1
Immunocytochemistry MAP2 NB300-213
Enlarge
5
reviewed by:
Karthik Krishnamurthy
ICC Rat 11/01/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedCultured motor neurons
SpeciesRat
Lot7225-9

Comments

CommentsUsed at a dilution of 1:2500. incubated at 4 degrees overnight. Robust staining
Immunocytochemistry MAP2 NB300-213
Enlarge
5
reviewed by:
Lingling Yang
ICC Mouse 11/22/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse primary cultures
SpeciesMouse

Comments

Comments1:1000 dilution
Immunofluorescence MAP2 NB300-213
Enlarge
5
reviewed by:
Verified Customer
IF Human 01/22/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedRat and Human.
SpeciesHuman
  5
reviewed by:
Michael Olive
IHC-Fr Rat 12/07/2010
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRat brain tissue
SpeciesRat

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MAP2 Antibody (NB300-213). (Showing 1 - 3 of 3 FAQs).

  1. Can you please tell me the country of origin for antibody NB300-213, chicken anti-MAP2?
    • Our product NB300-213 originates in the United States.
  2. Would you be able to share the concentration of NB300-213 from Lot# 7214, and the current lot of this antibody?
    • This antibody is a chicken polyclonal that has been precipitated therefore a concentration is irrelevant. This is because, unlike an affinity purified antibody, there will be other proteins that absorb at 280 that are not the specific antibody to the protein target. The concentration is usually about 20mg/ml. We currently are on Lot# 7225-3.
  3. I was using your product NB300-213 for MAP2 immunofluorescence, I did many times, which showed a beaded signal consistently unlike the picture presented on the product datasheet,could you give me some advice?
    • I looked at the images, and I think that the MAP2 antibody is working fine. The beading of the dendrites is likely due to some problem with fixation, which resulted in some damage to the neurons. MAP2 is bound to microtubules which are very labile, rapidly breaking down in the cold or if the cells are metabolically or mechanically challenged. When this happens, the dendrites take up a beaded appearance, as in the images. We routinely use a fixation protocol in which cells are fixed briefly in formalin at room temperature, which stabilizes the microtubules, followed by cold methanol, which helps to permeabilize the cells.

Secondary Antibodies

 

Isotype Controls

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Research Areas for MAP2 Antibody (NB300-213)

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Blogs on MAP2.

Methamphetamine with HIV induces mitochondrial dysfunction and neuronal injury through oxidative stress
By Jamshed Arslan, Pharm. D., PhD. December 1 is the World AIDS Day. Despite the combination antiretroviral therapy, 10-25% of Human Immunodeficiency Virus (HIV)-positive individuals report neurocognitive impairm...  Read full blog post.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
 By Jamshed Arslan Pharm.D.  Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...  Read full blog post.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

mFluor Violet Conjugated Antibodies

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Recent Reviews

5
5
7
4
0
3
0
2
0
1
0

Shashirekha Markandaiah
12/08/2022
Application: ICC
Species: Human

Verified Customer
11/19/2022
Application: IHC-Fr
Species: Feline

Verified Customer
07/29/2021
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol MAP2
Entrez
Uniprot