MAP2 Antibody


Western Blot: MAP2 Antibody [NB300-213] - Western blot analysis of MAP2 expression in bovine brain extract using NB300-213.
Immunocytochemistry/ Immunofluorescence: MAP2 Antibody [NB300-213] - Mixed neuron/glial cultures stained with NB300-213 (red). The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 more
Immunofluorescence: MAP2 Antibody [NB300-213] - Human DA neurons 28 DIV stained with MAP2 antibody (blue). Image from verified customer review.

Product Details

Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

MAP2 Antibody Summary

Purified MAP2 isolated from bovine brain.
Neuronal Dendritic Marker
Ammonium sulfate precipitation
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
5mM Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
Ammonium sulfate precipitation


Application Notes
This MAP2 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen sections and Western blot. In Western blot bands can be seen at approximately 280 kDa, representing MAP2a and MAP2b. Bands may also be seen at approximately 70 kDa, representing MAP2c. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
280 kDa
Reviewed Applications
Read 2 Reviews rated 5
NB300-213 in the following application:

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NB300-213 in the following applications:

Reactivity Notes

Human, mouse, rat and bovine. Other species have not been tested.

Alternate Names for MAP2 Antibody

  • DKFZp686I2148
  • MAP-2
  • MAP2A
  • MAP2B
  • MAP2C
  • microtubule-associated protein 2


MAP2 belongs to a family of proteins known as the microtubule associated proteins (MAPs). MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. Antibodies to MAP2 are excellent markers on neuronal cells, their perikarya and neuronal dendrites. Dendrite specific MAP is found specifically in the dendritic branching of neurons.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MAP2 Antibody (NB300-213)(23)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-Fr.

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Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NB300-213 Applications Species
Ciaraldi TP, Ryan AJ, Mudaliar SR, Henry RR. Altered Myokine Secretion Is an Intrinsic Property of Skeletal Muscle in Type 2 Diabetes PLoS ONE 2016 Jul 26 [PMID:27453994] (ICC/IF, Human) ICC/IF Human
Verma N, Ly H, Liu M et al. Intraneuronal Amylin Deposition Peroxidative Membrane Injury and Increased IL-1beta Synthesis in Brains of Alzheimer's Disease Patients with Type-2 Diabetes and in Diabetic HIP Rats. J. Alzheimers Dis. May 5 2016 12:00AM [PMID:27163815] (ICC/IF, Rat) ICC/IF Rat
Haas LT, Salazar SV, Kostylev MA et al. Metabotropic glutamate receptor 5 couples cellular prion protein to intracellular signalling in Alzheimer's disease. Brain. 2015 Dec 14 [PMID:26667279] (ICC/IF, Mouse) ICC/IF Mouse
Zhou H, Liu G. Regulation of density of functional presynaptic terminals by local energy supply. Mol Brain 2015 [PMID:26184109] (ICC/IF) ICC/IF
Usui Y, Westenskow PD, Kurihara T et al. Neurovascular crosstalk between interneurons and capillaries is required for vision. J. Clin. Invest. 2015 Jun 01 [PMID:25915585] (Mouse) Mouse
Schwerk A, Altschuler J, Roch M et al. Human adipose-derived mesenchymal stromal cells increase endogenous neurogenesis in the rat subventricular zone acutely after 6-hydroxydopamine lesioning. Cytotherapy. 2014 Oct 24 [PMID:25457280] (IHC-Fr, Human) IHC-Fr Human
Oenarto J, Gorg B, Moos M et al. Expression of organic osmolyte transporters in cultured rat astrocytes and rat and human cerebral cortex. Arch. Biochem. Biophys. 2014 Jul 05 [PMID:25004465] (ICC/IF, IHC-Fr, Rat, Human) ICC/IF, IHC-Fr Rat, Human
Khacho M, Tarabay M, Patten D et al. Acidosis overrides oxygen deprivation to maintain mitochondrial function and cell survival. Nat Commun 2014 Apr 01 [PMID:24686499] (ICC/IF, Mouse) ICC/IF Mouse
Nieland Thomas J F, Logan David J, Saulnier Jessica et al. High content image analysis identifies novel regulators of synaptogenesis in a high-throughput RNAi screen of primary neurons. PLoS One. 2014 Mar 14 [PMID:24633176] (ICC/IF, Mouse) ICC/IF Mouse
Sharma K, Choi SY, Zhang Y et al. High-Throughput Genetic Screen for Synaptogenic Factors: Identification of LRP6 as Critical for Excitatory Synapse Development. Cell Rep 2013 Dec 12 [PMID:24316074] (ICC/IF, Mouse) ICC/IF Mouse
Show All 23 Publications.

Reviews for MAP2 Antibody (NB300-213) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rat.
We have 2 review tested in 1 application: IHC-Fr.

Reviews using NB300-213:
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Images Ratings Applications Species Date Details
 MAP2 NB300-213
reviewed by:
Human 01/22/2016


Sample TestedRat and Human.


Blocking Details2%BSA, 2% Donekey serum, 0.1% Triton X100

Primary Anitbody

Dilution Ratio1:2000 in block

Secondary Antibody

Secondary DescriptionDonkey anti Chick-Alexa 647
Secondary Manufacturer Cat#JacksonImmuno
Secondary Concentration1:800


Detection NotesImaged on Leica SP5 confocal microscope
Fixation DetailsPFA/MetOH fixed.
Wash DescriptionPBS 3X
reviewed by:
IHC-Fr Rat 12/07/2010


Sample TestedRat brain tissue
CommentsGreat staining patterns!


Blocking DetailsBlocking Buffer: 5% donkey serum, Blocking Time: 2 hours, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1:1000, Incubation Dilution Buffer: PBS + 0.1% Tween + 5% donkey serum, Incubation Time: overnight, Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Dylight488 donkey anti-rabbit, Secondary Ab Dilution: 1:500


Detection NotesDetection Method: epifluorescence
Fixation DetailsSample Preparation: OCT embedded Cryosection


CommentsGreat staining patterns!

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MAP2 Antibody (NB300-213). (Showing 1 - 1 of 1 FAQs).

  1. Can you please tell me the country of origin for antibody NB300-213, chicken anti-MAP2?
    • Our product NB300-213 originates in the United States.

Secondary Antibodies


Isotype Controls

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Blogs on MAP2.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

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Product PDFs


Gene Symbol MAP2

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