MUC1 Antibody (SM3)


Immunocytochemistry/ Immunofluorescence: MUC-1 Antibody (SM3) [NB120-22711] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were more
Immunohistochemistry-Paraffin: MUC-1 Antibody (SM3) [NB120-22711] - IHC analysis of formalin fixed paraffin embedded tissue section of human breast cancer xenograft using MUC-1 antibody clone SM3 at 1:10 dilution. The more
Immunocytochemistry/ Immunofluorescence: MUC-1 Antibody (SM3) [NB120-22711] - MUC-1 antibody was tested in MCF7 cells at 10 ug/ml using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) more
Immunohistochemistry-Paraffin: MUC-1 Antibody (SM3) [NB120-22711] - IHC analysis of formalin fixed paraffin embedded tissue section of human breast cancer xenograft using MUC-1 antibody clone SM3 at 1:10 dilution. The more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
1.0 mg/ml

Order Details

MUC1 Antibody (SM3) Summary

Hydrogen fluoride deglycosylated milk mucin.
Cell Membrane and Cytoplasmic
Reacts very little with normal tissue. Found to react with breast, colon and ovarian carcinomas and adinocarcinoma. SM3 recognises the under-glycosylated form of MUC1 and is therefore tumour specific.
IgG2a Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
IHC-Fr and IHC-P: Use at an assay dependent dilution. Use in ELISA, and FLOW reported in various pieces of scientific literature.
Read Publications using
NB120-22711 in the following applications:

  • 4 publications
  • 4 publications
  • IHC
    3 publications

Reactivity Notes

Human. Mouse reactivity reported in scientific literature (PMID: 1372533) Not record of testing in other species.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for MUC1 Antibody (SM3)

  • Breast carcinoma-associated antigen DF3
  • Carcinoma-associated mucin
  • CD227 antigen
  • CD227
  • DF3 antigen
  • EMA
  • Episialin
  • H23 antigen
  • H23AG
  • KL-6
  • MAM6
  • MUC-1
  • MUC1/ZD
  • mucin 1, cell surface associated
  • mucin 1, transmembrane
  • Mucin-1
  • Peanut-reactive urinary mucin
  • PEM
  • PEMT
  • Polymorphic epithelial mucin
  • PUMMUC-1/X
  • tumor associated epithelial mucin
  • Tumor-associated epithelial membrane antigen
  • Tumor-associated mucin


MUC-1, also known as mucin 1, is a large cell surface mucin glycoprotein involved in cell adhesion and tumor progression. Specifically, MUC-1 is responsible for the formation of protective mucous barriers on epithelial surfaces and plays a role in intracellular signaling. MUC-1 is proteolytically cleaved to form a heterodimeric complex consisting of both an N-terminal alpha subunit and C-terminal beta subunit. The alpha subunit plays a role in cell adhesion whereas the beta subunit has involvement in cell signaling. MUC-1 is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues. It is found in high abundance in airway passages, breast, uterine, stomach and pancrean tissue. As it is overexpressed in epithelial tumors, MUC-1 is linked to such diseases as breast and ovarian cancer.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MUC1 Antibody (NB120-22711)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ELISA, FLOW, IHC.

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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB120-22711 Applications Species
Sorensen AL, Reis CA, Tarp MA et al. Chemoenzymatically synthesized multimeric Tn/STn MUC1 glycopeptides elicit cancer-specific anti-MUC1 antibody responses and override tolerance. Glycobiology. 2006 Feb 16 [PMID: 16207894] (ELISA, Human) ELISA Human
Dalziel M, Whitehouse C, McFarlane I et al. The relative activities of the C2GnT1 and ST3Gal-I glycosyltransferases determine O-glycan structure and expression of a tumor-associated epitope on MUC1. J Biol Chem 2001 Apr 6 [PMID: 11118434] (FLOW, Human) FLOW Human
Mommers EC, Leonhart AM, von Mensdorff-Pouilly S et al. Aberrant expression of MUC1 mucin in ductal hyperplasia and ductal carcinoma In situ of the breast. Int J Cancer. 1999 Oct 22 [PMID: 10502721] (IHC, Human) IHC Human
Henderikx P, Kandiloqiannaki M, Petrarca C et a.l. Human single-chain Fv antibodies to MUC1 core peptide selected from phage display libraries recognize unique epitopes and predominantly bind adenocarcinoma. Cancer Res. 1998 Oct 1 [PMID: 9766660] (FLOW, Human) FLOW Human
Karsten U, Diotel c, Klich g et al. Enhanced binding of antibodies to the DTR motif of MUC1 tandem repeat peptide is mediated by site-specific glycosylation. Cancer Res. 1998 Jun 15 [PMID: 9635576] (ELISA, Human) ELISA Human
Bohm CM, Mulder MC, Zennadi R et al. Carbohydrate recognition on MUC1-expressing targets enhances cytotoxicity of a T cell subpopulation. Scnd J Immunol. 1997 Jul [PMID: 9246205] (FLOW, Human) FLOW Human
Hanisch FG, Stadie TR, Deutzmann F et al. MUC1 glycoforms in breast cancer--cell line T47D as a model for carcinoma-associated alterations of 0-glycosylation. Eur J Biochem. 1996 Feb 15 [PMID: 8617281] (ELISA, Human) ELISA Human
Ho Jj, Siddiki B, Kim YS et al. Association of sialyl-Lewis(a) and sialyl-Lewis(x) with MUC-1 apomucin ina pancreatic cancer cell line. Cancer Res. 1995 Aug 15 [PMID: 7627976] (ELISA, Human) ELISA Human
Brockhausen I, Yang JM Burchell J et al. Mechanisms underlying aberrant glycosylation of MUC1 mucin in breast cancer cells Eur J Biochem. 1995 Oct 15 [PMID: 7588808] (FLOW, Human) FLOW Human
Kotera Y, Fontenot JD, Pecher G et al. Humoral immunity against a tandem repeat epitope of human mucin MUC-1 in sera from breast, pancreatic, and colon cancer patients. Cancer Res. 1994 Jun 1 [PMID: 7514493] (IHC, Human) IHC Human
Show All 11 Publications.

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Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for MUC1 Antibody (NB120-22711). (Showing 1 - 1 of 1 FAQs).

  1. I would like to perform a sandwich assay for the determination of MUC1 protein. May you suggest me some antibodies that can be used together? In particular, if possible, I should need antibodies product in mouse or rabbit.
    • Unfortunately we are not aware of a pair of antibodies that have been used in sandwich ELISA in particular. If you are interested in trying two you may find our Innovators Reward Program to be helpful.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB120-22711AF350
Alexa Fluor 405 NB120-22711AF405
Alexa Fluor 488 NB120-22711AF488
Alexa Fluor 532 NB120-22711AF532
Alexa Fluor 594 NB120-22711AF594
Alexa Fluor 647 NB120-22711AF647
Alexa Fluor 700 NB120-22711AF700
Alexa Fluor 750 NB120-22711AF750
Allophycocyanin/Cy7 NB120-22711APCCY7
Biotin NB120-22711B
DyLight 350 NB120-22711UV
DyLight 405 NB120-22711V
DyLight 488 NB120-22711G
DyLight 550 NB120-22711R
DyLight 594 NB120-22711DL594
DyLight 650 NB120-22711C
DyLight 680 NB120-22711FR
DyLight 755 NB120-22711IR
FITC NB120-22711F
HRP NB120-22711H
Janelia Fluor 549 NB120-22711JF549
Janelia Fluor 646 NB120-22711JF646
PE/Atto594 NB120-22711PEATT594
PE/Cy5.5 NB120-22711PECY55
PE/Cy7 NB120-22711PECY7

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Gene Symbol MUC1