Human Free BDNF Quantikine ELISA Kit

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Reactivity HuSpecies Glossary
Applications ELISA

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Human Free BDNF Quantikine ELISA Kit Summary

The Quantikine Human BDNF Immunoassay is a 3.5 hour solid phase ELISA designed to measure human BDNF in cell culture supernates, serum, and plasma. It contains recombinant human BDNF expressed in Sf 21 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant BDNF accurately. Results obtained measuring natural human BDN...F showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human BDNF.
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Natural and recombinant human BDNF
Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
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Sample Volume
See PDF Datasheet for details


Application Notes
Interference observed with 1 or more available related molecules.
Read Publications using
DBD00 in the following applications:

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Free BDNF Quantikine ELISA Kit

  • Abrineurin
  • ANON2
  • BDNF
  • brain-derived neurotrophic factor
  • BULN2
  • MGC34632
  • Neurotrophin


BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for BDNF (DBD00)(59)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, N/A, Transgenic Mouse.

We have publications tested in 1 application: ELISA Capture.

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Showing Publications 1 - 10 of 59. Show All 59 Publications.
Publications using DBD00 Applications Species
FJ Raucci, AP Singh, J Soslow, LW Markham, L Zhong, W Aljafar, N Lessiohadi, CP Awgulewits, P Umbarkar, Q Zhang, PL Cannon, M Buchowski, JT Roland, EJ Carrier, WB Burnette, AK Hatzopoulo, H Lal, CL Galindo The BDNF rs6265 Polymorphism is a Modifier of Cardiomyocyte Contractility and Dilated Cardiomyopathy Int J Mol Sci, 2020;21(20):. 2020 [PMID: 33050457] (Human) Human
Z Piotrowicz, M Chalimoniu, K P?oszczyca, M Czuba, J Langfort Exercise-Induced Elevated BDNF Level Does Not Prevent Cognitive Impairment Due to Acute Exposure to Moderate Hypoxia in Well-Trained Athletes Int J Mol Sci, 2020;21(15):. 2020 [PMID: 32759658] (Human) Human
C Rezola-Par, G Hervás, H Arrieta, AH Diego, F Ruiz-Litag, SM Gil, A Rodriguez-, J Irazusta Physical exercise interventions have no effect on serum BDNF concentration in older adults living in long-term nursing homes Exp. Gerontol., 2020;139(0):111024. 2020 [PMID: 32693009] (Human) Human
C Wu, J Lu, S Lu, M Huang, Y Xu Increased ratio of mature BDNF to precursor-BDNF in patients with major depressive disorder with severe anhedonia J Psychiatr Res, 2020;126(0):92-97. 2020 [PMID: 32428748] (Human) Human
NF Kasim, JV van Zanten, S Aldred Tai Chi is an effective form of exercise to reduce markers of frailty in older age Exp. Gerontol., 2020;135(0):110925. 2020 [PMID: 32184194] (Human) Human
J Nilsson, Ö Ekblom, M Ekblom, A Lebedev, O Tarassova, M Moberg, M Lövdén Acute increases in brain-derived neurotrophic factor in plasma following physical exercise relates to subsequent learning in older adults Sci Rep, 2020;10(1):4395. 2020 [PMID: 32157099] (Human) Human
KL Szuhany, MW Otto Assessing BDNF as a mediator of the effects of exercise on depression J Psychiatr Res, 2020;123(0):114-118. 2020 [PMID: 32065946] (Human) Human
H Arrieta, C Rezola-Par, M Kortajaren, G Hervás, J Gil, JJ Yanguas, M Iturburu, SM Gil, J Irazusta, A Rodriguez- The impact of physical exercise on cognitive and affective functions and serum levels of brain-derived neurotrophic factor in nursing home residents: A randomized controlled trial Maturitas, 2020;131(0):72-77. 2020 [PMID: 31787150] (Human) Human
D Hao, C He, B Ma, L Lankford, L Reynaga, DL Farmer, F Guo, A Wang Hypoxic Preconditioning Enhances Survival and Proangiogenic Capacity of Human First Trimester Chorionic Villus-Derived Mesenchymal Stem Cells for Fetal Tissue Engineering Stem Cells Int, 2019;2019(0):9695239. 2019 [PMID: 31781252] (Human) Human
F Scarpina, P Marzullo, S Mai, A Mauro, M Scacchi, M Costantini Altered temporal sensitivity in obesity is linked to pro-inflammatory state Sci Rep, 2019;9(1):15508. 2019 [PMID: 31664059] (Human) Human
Show All 59 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for BDNF (DBD00). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Blogs on BDNF. Showing 1-10 of 11 blog posts - Show all blog posts.

  Read full blog post.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Niemann Pick-C1 and cholesterol dynamics
Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis.  Although cholesterol moves freely inside the cell, it cannot independently expo...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its...  Read full blog post.

TrkB and Nervous System Function
Neutrophins and their receptors play an important role in regulating the development of both the central and peripheral nervous systems. Neurotrophin ligand binding to each of their respective Trk cellular receptors is essential for the growth and sur...  Read full blog post.

TrkB: Docking for Neurotrophins and Beyond.
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small pro...  Read full blog post.

BDNF Antibodies Aid Research on Alzheimer's Therapies
Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

BDNF Antibodies and Synaptic Research
Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophins. During development it regulates the survival and differentiation of neuronal cell populations in the central and peripheral nervous system, while in adult synapse...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.

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Gene Symbol BDNF