Human Free BDNF Quantikine ELISA Kit

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Reactivity HuSpecies Glossary
Applications ELISA

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Human Free BDNF Quantikine ELISA Kit Summary

The Quantikine Human BDNF Immunoassay is a 3.5 hour solid phase ELISA designed to measure human BDNF in cell culture supernates, serum, and plasma. It contains recombinant human BDNF expressed in Sf 21 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant BDNF accurately. Results obtained measuring natural human BDN...F showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human BDNF.
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Natural and recombinant human BDNF
Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
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Sample Volume
See PDF Datasheet for details


Application Notes
Interference observed with 1 or more available related molecules.
Read Publications using
DBD00 in the following applications:

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Free BDNF Quantikine ELISA Kit

  • Abrineurin
  • ANON2
  • BDNF
  • brain-derived neurotrophic factor
  • BULN2
  • MGC34632
  • Neurotrophin


BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for BDNF (DBD00)(97)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 1 application: ELISA Capture.

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Showing Publications 1 - 10 of 97. Show All 97 Publications.
Publications using DBD00 Applications Species
Li, H;Zhao, M;Jiang, C;Zhao, H;Wu, C;Li, Y;Zhang, S;Xu, P;Mou, T;Xu, Y;Huang, M; Elevated Plasma Levels of Mature Brain-Derived Neurotrophic Factor in Major Depressive Disorder Patients with Higher Suicidal Ideation Brain sciences 2023-08-21 [PMID: 37626579] (Human) Human
Nilsson, J;Ekblom, M;Moberg, M;Lövdén, M; The role of acute changes in mBDNF, cortisol and pro-BDNF in predicting cognitive performance in old age Scientific reports 2023-06-09 [PMID: 37296176] (Human) Human
M ?miarowska, M Pawlicka, A Boro?, A Grzywacz, K Safranow, D Chlubek, V Dziedziejk Association between Psychopathological Symptoms and Aggression and Selected Biochemical Parameters in Adolescents with Behavioural and Emotional Disturbances International Journal of Molecular Sciences, 2023-04-12;24(8):. 2023-04-12 [PMID: 37108259] (Human) Human
L Hernandez, LJ Ward, S Arefin, P Barany, L Wennberg, M Söderberg, S Bruno, V Cantaluppi, P Stenvinkel, K Kublickien Blood-Brain Barrier Biomarkers before and after Kidney Transplantation International Journal of Molecular Sciences, 2023-04-01;24(7):. 2023-04-01 [PMID: 37047601] (Human) Human
M Nikolac Pe, F Borovecki, I Filipcic, B Vuic, T Milos, G Nedic Erja, M Konjevod, L Tudor, N Mimica, S Uzun, O Kozumplik, D Svob Strac, N Pivac Relationship between Brain-Derived Neurotrophic Factor and Cognitive Decline in Patients with Mild Cognitive Impairment and Dementia Biomolecules, 2023-03-21;13(3):. 2023-03-21 [PMID: 36979505] (Human) Human
M Bode, P Sulzer, C Schulte, S Becker, K Brockmann, S Elben, AK Folkerts, A Ophey, C Schlensted, K Witt, L Wojtecki, J Evers, W Maetzler, E Kalbe, I Liepelt-Sc Multidomain cognitive training increases physical activity in people with Parkinson's disease with mild cognitive impairment Parkinsonism & related disorders, 2023-02-17;0(0):105330. 2023-02-17 [PMID: 36842867] (Human) Human
CY Hsu, WH Sheu, IT Lee Brain-derived neurotrophic factor associated with kidney function Diabetology & metabolic syndrome, 2023-02-13;15(1):16. 2023-02-13 [PMID: 36782254] (Human) Human
M Irfan, S Chung C5L2 modulates BDNF production in human dental pulp stem cells via p38alpha pathway Scientific Reports, 2023-01-02;13(1):74. 2023-01-02 [PMID: 36593314] (Human) Human
A Ullrich, K Wenzel, M Bahls, L Voigt, S Könemann, M Dörr, S Wurm, S Ulbricht Preliminary results of the cross-sectional associations of sedentary behavior and physical activity with serum brain-derived neurotrophic factor in adults with coronary heart disease Scientific Reports, 2022-11-16;12(1):19685. 2022-11-16 [PMID: 36385629] (Human) Human
V Sabrina, C Carmen, A Gloria, B Elisa, C Nico, B Marina, F Luigi, V Piera Impact of Freeze-Drying on Cord Blood (CB), Serum (S), and Platelet-Rich Plasma (CB-PRP) Preparations on Growth Factor Content and In Vitro Cell Wound Healing International Journal of Molecular Sciences, 2022-09-14;23(18):. 2022-09-14 [PMID: 36142617] (Human) Human
Show All 97 Publications.

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Blogs on BDNF. Showing 1-10 of 11 blog posts - Show all blog posts.

  Read full blog post.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Niemann Pick-C1 and cholesterol dynamics
Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis.  Although cholesterol moves freely inside the cell, it cannot independently expo...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its ...  Read full blog post.

TrkB and Nervous System Function
Neutrophins and their receptors play an important role in regulating the development of both the central and peripheral nervous systems. Neurotrophin ligand binding to each of their respective Trk cellular receptors is essential for the growth and sur...  Read full blog post.

TrkB: Docking for Neurotrophins and Beyond.
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small pro...  Read full blog post.

BDNF Antibodies Aid Research on Alzheimer's Therapies
Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

BDNF Antibodies and Synaptic Research
Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophins. During development it regulates the survival and differentiation of neuronal cell populations in the central and peripheral nervous system, while in adult synapse...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.

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Gene Symbol BDNF