Human Free BDNF Quantikine ELISA Kit

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

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Catalog# & Conjugate Size Price

Human Free BDNF Quantikine ELISA Kit Summary

Background
The Quantikine Human BDNF Immunoassay is a 3.5 hour solid phase ELISA designed to measure human BDNF in cell culture supernates, serum, and plasma. It contains recombinant human BDNF expressed in Sf 21 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant BDNF accurately. Results obtained measuring natural human BDN...F showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human BDNF.
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Specificity
Natural and recombinant human BDNF
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
BDNF

Applications/Dilutions

Application Notes
Interference observed with 1 or more available related molecules.
Publications
Read Publications using
DBD00 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Free BDNF Quantikine ELISA Kit

  • Abrineurin
  • ANON2
  • BDNF
  • brain-derived neurotrophic factor
  • BULN2
  • MGC34632
  • Neurotrophin

Background

BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for BDNF (DBD00)(53)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, N/A, Transgenic Mouse.

We have publications tested in 1 application: ELISA Capture.


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ELISA Capture
(1)
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Human
(44)
Mouse
(1)
Rat
(5)
N/A
(1)
Transgenic Mouse
(1)
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Showing Publications 1 - 10 of 53. Show All 53 Publications.
Publications using DBD00 Applications Species
C Wu, J Lu, S Lu, M Huang, Y Xu Increased ratio of mature BDNF to precursor-BDNF in patients with major depressive disorder with severe anhedonia J Psychiatr Res, 2020;126(0):92-97. 2020 [PMID: 32428748] (Human) Human
H Arrieta, C Rezola-Par, M Kortajaren, G Hervás, J Gil, JJ Yanguas, M Iturburu, SM Gil, J Irazusta, A Rodriguez- The impact of physical exercise on cognitive and affective functions and serum levels of brain-derived neurotrophic factor in nursing home residents: A randomized controlled trial Maturitas, 2020;131(0):72-77. 2020 [PMID: 31787150] (Human) Human
D Hao, C He, B Ma, L Lankford, L Reynaga, DL Farmer, F Guo, A Wang Hypoxic Preconditioning Enhances Survival and Proangiogenic Capacity of Human First Trimester Chorionic Villus-Derived Mesenchymal Stem Cells for Fetal Tissue Engineering Stem Cells Int, 2019;2019(0):9695239. 2019 [PMID: 31781252] (Human) Human
F Scarpina, P Marzullo, S Mai, A Mauro, M Scacchi, M Costantini Altered temporal sensitivity in obesity is linked to pro-inflammatory state Sci Rep, 2019;9(1):15508. 2019 [PMID: 31664059] (Human) Human
ML Schmalhofe, MRP Markus, JC Gras, J Kopp, D Janowitz, HJ Grabe, S Gro beta, R Ewert, S Gläser, D Albrecht, I Eiffler, H Völzke, N Friedrich, M Nauck, A Steveling, S Könemann, K Wenzel, SB Felix, M Dörr, M Bahls Sex-Specific Associations of Brain-Derived Neurotrophic Factor and Cardiorespiratory Fitness in the General Population Biomolecules, 2019;9(10):. 2019 [PMID: 31635145] (Human) Human
R Lejkowska, MP Kawa, E Pius-Sadow, D Rogi?ska, K ?uczkowska, B Machali?sk, A Machali?sk Preclinical Evaluation of Long-Term Neuroprotective Effects of BDNF-Engineered Mesenchymal Stromal Cells as Intravitreal Therapy for Chronic Retinal Degeneration in Rd6 Mutant Mice Int J Mol Sci, 2019;20(3):. 2019 [PMID: 30759764] (Transgenic Mouse) Transgenic Mouse
F Russo, G Chimienti, G Riezzo, M Linsalata, B D'Attoma, C Clemente, A Orlando Adipose Tissue-Derived Biomarkers of Intestinal Barrier Functions for the Characterization of Diarrhoea-Predominant IBS Dis. Markers, 2018;2018(0):1827937. 2018 [PMID: 30622656] (Human) Human
MH Lin, CY Chung, KT Chen, JC Yeh, TH Lee, MH Lee, IN Lee, WC Huang, JT Yang Comparison between Polybutylcyanoacrylate Nanoparticles with Either Surface-Adsorbed or Encapsulated Brain-Derived Neurotrophic Factor on the Neural Differentiation of iPSCs Int J Mol Sci, 2019;20(1):. 2019 [PMID: 30621332] (N/A) N/A
J Jaworska, K Micielska, M Koz?owska, K Wnorowski, J Skrobecki, L Radzimi?sk, A Babi?ska, E Rodziewicz, G Lombardi, E Ziemann A 2-Week Specific Volleyball Training Supported by the Whole Body Cryostimulation Protocol Induced an Increase of Growth Factors and Counteracted Deterioration of Physical Performance Front Physiol, 2018;9(0):1711. 2018 [PMID: 30555349] (Human) Human
TT Yeh, KC Chang, CY Wu, YY Lee, PY Chen, JW Hung Effects and mechanism of the HECT study (hybrid exercise-cognitive trainings) in mild ischemic stroke with cognitive decline: fMRI for brain plasticity, biomarker and behavioral analysis Contemp Clin Trials Commun, 2018;9(0):164-171. 2018 [PMID: 29696239] (Human) Human
Show All 53 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for BDNF (DBD00). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP DBD00
HRP DBD00
HRP DBD00

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Bioinformatics Tool for BDNF (DBD00)

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Blogs on BDNF. Showing 1-10 of 11 blog posts - Show all blog posts.

Gut-brain axis: microbiota influence behavior and mental well-being
By Jennifer Sokolowski, MD, PhD.Gut-microbiome interactionsOn and in our bodies, microbes outnumber our cells by about ten-to-one. Studies have revealed that the microbiome influences neurogenesis, cognition, and...  Read full blog post.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Niemann Pick-C1 and cholesterol dynamics
Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis.  Although cholesterol moves freely inside the cell, it cannot independently expo...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its...  Read full blog post.

TrkB and Nervous System Function
Neutrophins and their receptors play an important role in regulating the development of both the central and peripheral nervous systems. Neurotrophin ligand binding to each of their respective Trk cellular receptors is essential for the growth and sur...  Read full blog post.

TrkB: Docking for Neurotrophins and Beyond.
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small pro...  Read full blog post.

BDNF Antibodies Aid Research on Alzheimer's Therapies
Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

BDNF Antibodies and Synaptic Research
Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophins. During development it regulates the survival and differentiation of neuronal cell populations in the central and peripheral nervous system, while in adult synapse...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.
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Bioinformatics

Gene Symbol BDNF