Survivin Antibody (60.11) - Unpurified (NB500-205)


Western Blot: Survivin Antibody (60.11) [NB500-205] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY Survivin cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were more
Immunocytochemistry/ Immunofluorescence: Survivin Antibody (60.11) [NB500-205] - Detection of Survivin protein (Green) in Hela cells using NB500-238. Nuclei(Blue) were counterstained using Hoechst 33258.
Immunohistochemistry-Paraffin: Survivin Antibody (60.11) [NB500-205] - Staining of ovary cancer.
Flow Cytometry: Survivin Antibody (60.11) [NB500-205] - Staining of NTERA-2 cells using NB500-205 at a 1:2000 dilution detected using Dylight-488 conjugated goat anti-mouse IgG secondary antibody.
Western Blot: Survivin Antibody (60.11) [NB500-205] - Survivin detected in rat aorta smooth muscle cell lysate.
Western Blot: Survivin Antibody (60.11) [NB500-205] - Detection in 30ug of HeLa whole cell extract using NB500-238 at 1ug/ml.
Immunocytochemistry/ Immunofluorescence: Survivin Antibody (60.11) [NB500-205] - HeLa cells stained NB500-205 (Green) detected with DyLight Fluor 488 conjugated anti-mouse IgG secondary antibody. Nuclei are more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP

Survivin Antibody (60.11) - Unpurified Summary

Full length recombinant human Survivin [UniProt# O15392]
The epitope recognized is between amino acids 57-67.
NB500-205 is specific for the cytoplasmic form of survivin.
IgG2a Kappa
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  • Western Blot
  • Flow Cytometry 1:200
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
Application Notes
This Survivin (60.11) antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. Immunohistochemistry-Paraffin and Western Blot were reported in scientific literature. This antibody weakly detects Survivin in Western blot; for this application we suggest using NB500-235 instead. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 22841823) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
16.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
Survivin Lysate (NBL1-07987)
Read Publications using
NB500-205 in the following applications:

Reactivity Notes

Human, mouse and rat

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Ascites with PBS
0.05% Sodium Azide

Alternate Names for Survivin Antibody (60.11) - Unpurified

  • API4 survivin variant 3 alpha
  • API4
  • Apoptosis inhibitor 4 baculoviral IAP repeat-containing protein 5
  • Apoptosis inhibitor survivin
  • baculoviral IAP repeat-containing 5
  • BIRC5
  • EPR-1
  • IAP4
  • Survivin


Survivin (BIRC5 or IAP4) is the smallest member of inhibitors of apoptosis proteins (X-linked IAP/XIAP, c-IAP1, c-IAP2, IAP-like protein-2/ ILP2, melanoma IAP/ ML-IAP, Livin, NAIP, and survivin) and besides its anti-apoptotic functions; survivin also regulates processes such as cell cycle, migration, metastasis etc. Highly critical in fetal development, survivin is generally no longer present in adult tissues; however, its re-expression occurs in cancers wherein it drives proliferation, metastasis, poor prognosis, and decreased patient survival. Survivin contains only one copy of a modified baculovirus IAP repeat (BIR, a domain required for caspases inactivation) which is used for homodimerization and for its interaction with other chromosome passenger proteins. Phosphorylation/mutation of Thr-48 in the BIR domain affects survivin's dual ability to inhibit apoptosis and regulate cell division. Under apoptotic stimuli involving mitochondria, survivin forms a complex with XIAP, thus increasing the stability of XIAP and its inhibitory activity against caspases. The survivin binding to mitochondrial Smac/DIABLO leads to a delayed Smac/DIABLO release in the cytoplasm resulting in prolonged cell survival. Survivin-HBXIP complex can bind to pro-caspase-9 for exerting prevention of mitochondrial apoptosis activation. For facilitating correct cell division, survivin recognize/complex with phospho-histone-H3 and associates with the chromosome passenger proteins (Aurora B, INCENP, and borealin) and this new complex is recruited to the mitotic centromers where it assists/ensure proper chromosomal segregation. Moreover, at the centrosomes of dividing cells, survivin binds to Cdk1 which is critical for cells to enter mitosis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Survivin Antibody (NB500-205)(20)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: ICC/IF, IHC-Fr, IHC-P, PLA, WB.

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Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NB500-205 Applications Species
Dheekollu J, Malecka K, Wiedmer A et al. Carcinoma-risk variant of EBNA1 deregulates Epstein-Barr Virus episomal latency. Oncotarget. Jan 31 2017 [PMID: 28077791] (PLA) PLA
Kapinas Kristina, Kim Heesun, Mandeville Matthew et al. microRNA-mediated survivin control of pluripotency. J Cell Physiol. 2014 [PMID: 24891298] (Human) Human
Hoel AW, Yu P, Nguyen KP, et al. Mitochondrial Heat Shock Protein-90 Modulates Vascular Smooth Muscle Cell Survival and the Vascular Injury Response in Vivo Am J Pathol. 2012 Oct [PMID: 22841823] (WB, IHC-Fr, Human) WB, IHC-Fr Human
de Souza HS, West GA, Rebert N et al. Increased Levels of Survivin, via Association With Heat Shock Protein 90, in Mucosal T Cells From Patients With Crohn's Disease Gastroenterology 2012 Jun 26 [PMID: 22749932] (Human) Human
Lechler P, Balakrishnan S, Schaumburger J, Grassel S, Baier C, Grifka J, Straub RH, Renkawitz T. The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation in vitro. BMC Musculoskelet Disord;12:150. 2011 Jul 5. [PMID: 21729321]
Knauer SK, Heinrich UR, Bier C et al. An otoprotective role for the apoptosis inhibitor protein survivin. Cell Death Dis 2010 Jul 1 [PMID: 21364656]
Habtemichael N, Wunsch D, Bier C et al. Cloning and functional characterization of the guinea pig apoptosis inhibitor protein Survivin. Gene 2010 Dec 1 [PMID: 20727954]
Habtemichael N, Heinrich UR, Knauer SK et al. Expression analysis suggests a potential cytoprotective role of Birc5 in the inner ear. Mol Cell Neurosci 2010 Nov [PMID: 20627126]
Huang H, Fletcher L, Beeharry N et al. Abnormal cytokinesis after X-irradiation in tumor cells that override the G2 DNA damage checkpoint. Cancer Res;68(10):3724-32. 2008 May 15. [PMID: 18483255] (ICC/IF, Human) ICC/IF Human
Levkau B, Schafers M, Wohlschlaeger J et al. Survivin determines cardiac function by controlling total cardiomyocyte number. Circulation. 117(12):1583-93. Epub 2008 Mar 10. Erratum in: Circulation. 2008 Jun 17;117(24):e497. Fischer, Jens. 2008 Mar 25. [PMID: 18332262] (WB, IHC-P, Human) WB, IHC-P Human
Show All 20 Publications.

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Product General Protocols

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ICC/IF Video Protocol

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Positive Control Lysate(s)

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Blogs on Survivin. Showing 1-10 of 14 blog posts - Show all blog posts.

Survivin - an inhibitor of apoptosis that drives tumorigenesis and metastasis
Untitled DocumentApoptosis is the tightly regulated process of programmed cell death. It plays an important role in normal physiologic development but has also been implicated in a number of diseases. Apoptosis is constantly downreg...  Read full blog post.

Survivin - an inhibitor of apoptosis protein
Survivin is an anti-apoptotic protein which is the smallest protein within a large family of proteins including X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, NAIP, and Livin. Survivin is responsible for a wide range of basic cellula...  Read full blog post.

Cytochrome C - a mediator of apoptosis
Cytochrome C is a small heme protein within the inner mitochondrial membrane responsible for carrying electrons within the respiratory transport chain.  Additionally, cytochrome c has also been identified as a player in programmed cell death (apop...  Read full blog post.

PCNA (Proliferating cell nuclear antigen, polymerase delta auxiliary protein)
PCNA is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It coordinates the recruitment and association of needed components during both of these processes, both of which are essential for cell cycl...  Read full blog post.

Survivin is thrivin'
The survivin anti-apoptotic protein is the smallest member of a large family of proteins such as X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates basic physiological events such as the cell cycle, tumor...  Read full blog post.

Livin: On a Prayer
Livin is a member of the inhibitor of apoptosis proteins (IAP) family that regulates programmed cell death. The Livin protein contains a single baculovirus IAP repeat (BIR) essential for function, along with a COOH-terminal RING-type zinc finger domai...  Read full blog post.

Survivin: As long as I know how to live I know I will
Survivin is an anti-apoptotic protein from a large family with related members such as X-linked IAP, cIAP1 and cIAP2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates fundamental physiological events including the cell cycle, feta...  Read full blog post.

Survivin: Infographic
Survivin is involved in promoting cell proliferation and is an inhibitor of apoptosis. Survivin has a critical role in cancer proliferation and neural development. It may have an impact on neural cell proliferative responses following brain injury.L...  Read full blog post.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its...  Read full blog post.

Caspase 7: The Cell's Suicide Switch
Caspase 7 (also known as CASP7, Mch3, ICE-LAP3, CMH-1) is a member of caspase family of cysteine proteases. It is an apoptosis-related cystein peptidase encoded by the CASP7 gene in humans. CASP7 homologous sequences have been identified in nearly all...  Read full blog post.

Showing 1-10 of 14 blog posts - Show all blog posts.

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