Western Blot: Survivin Antibody (60.11) [NB500-205] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY Survivin cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were ...read more
Immunocytochemistry/ Immunofluorescence: Survivin Antibody (60.11) [NB500-205] - Detection of Survivin protein (Green) in Hela cells using NB500-238. Nuclei(Blue) were counterstained using Hoechst 33258.
Immunohistochemistry-Paraffin: Survivin Antibody (60.11) [NB500-205] - Staining of ovary cancer.
Flow Cytometry: Survivin Antibody (60.11) [NB500-205] - Staining of NTERA-2 cells using NB500-205 at a 1:2000 dilution detected using Dylight-488 conjugated goat anti-mouse IgG secondary antibody.
Western Blot: Survivin Antibody (60.11) [NB500-205] - Survivin detected in rat aorta smooth muscle cell lysate.
Western Blot: Survivin Antibody (60.11) [NB500-205] - Detection in 30ug of HeLa whole cell extract using NB500-238 at 1ug/ml.
Immunocytochemistry/ Immunofluorescence: Survivin Antibody (60.11) [NB500-205] - HeLa cells stained NB500-205 (Green) detected with DyLight Fluor 488 conjugated anti-mouse IgG secondary antibody. Nuclei are ...read more
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Ascites with PBS
0.05% Sodium Azide
Flow Cytometry 1:200
This Survivin (60.11) antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. Immunohistochemistry-Paraffin and Western Blot were reported in scientific literature. This antibody weakly detects Survivin in Western blot; for this application we suggest using NB500-235 instead. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 22841823) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
16.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Apoptosis inhibitor 4 baculoviral IAP repeat-containing protein 5
Apoptosis inhibitor survivin
baculoviral IAP repeat-containing 5
Survivin (BIRC5 or IAP4) is the smallest member of inhibitors of apoptosis proteins (X-linked IAP/XIAP, c-IAP1, c-IAP2, IAP-like protein-2/ ILP2, melanoma IAP/ ML-IAP, Livin, NAIP, and survivin) and besides its anti-apoptotic functions; survivin also regulates processes such as cell cycle, migration, metastasis etc. Highly critical in fetal development, survivin is generally no longer present in adult tissues; however, its re-expression occurs in cancers wherein it drives proliferation, metastasis, poor prognosis, and decreased patient survival. Survivin contains only one copy of a modified baculovirus IAP repeat (BIR, a domain required for caspases inactivation) which is used for homodimerization and for its interaction with other chromosome passenger proteins. Phosphorylation/mutation of Thr-48 in the BIR domain affects survivin's dual ability to inhibit apoptosis and regulate cell division. Under apoptotic stimuli involving mitochondria, survivin forms a complex with XIAP, thus increasing the stability of XIAP and its inhibitory activity against caspases. The survivin binding to mitochondrial Smac/DIABLO leads to a delayed Smac/DIABLO release in the cytoplasm resulting in prolonged cell survival. Survivin-HBXIP complex can bind to pro-caspase-9 for exerting prevention of mitochondrial apoptosis activation. For facilitating correct cell division, survivin recognize/complex with phospho-histone-H3 and associates with the chromosome passenger proteins (Aurora B, INCENP, and borealin) and this new complex is recruited to the mitotic centromers where it assists/ensure proper chromosomal segregation. Moreover, at the centrosomes of dividing cells, survivin binds to Cdk1 which is critical for cells to enter mitosis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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