Survivin Antibody (60.11) (NB500-238)

Images

 
Western Blot: Survivin Antibody (60.11) [NB500-238] - Survivin detection in 30ug of HeLa whole cell extract using NB500-238 at 1ug/ml.
Immunocytochemistry/ Immunofluorescence: Survivin Antibody (60.11) [NB500-238] - Detection of Survivin protein (Green) in Hela cells using NB500-238. Nuclei(Blue) were counterstained using Hoechst 33258.
Immunohistochemistry-Paraffin: Survivin Antibody (60.11) [NB500-238] - Staining of ovary cancer.
Flow (Intracellular): Survivin Antibody (60.11) [NB500-238] - An intracellular stain was performed on Daudi cells with Survivin (60.11) antibody NB500-238 (blue) and a matched isotype control NB600-986 (orange). Cells ...read more
Western Blot: Survivin Antibody (60.11) [NB500-238] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY Survivin cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were ...read more
Western Blot: Survivin Antibody (60.11) [NB500-238] - Detected in rat aorta smooth muscle cell lysate.
Immunocytochemistry/ Immunofluorescence: Survivin Antibody (60.11) [NB500-238] - IHeLa cells stained NB500-205 (Green) detected with DyLight Fluor 488 conjugated anti-mouse IgG secondary antibody. Nuclei are ...read more
Flow Cytometry: Survivin Antibody (60.11) [NB500-238] - Staining of NTERA-2 cells using NB500-205 at a 1:2000 dilution detected using Dylight-488 conjugated goat anti-mouse IgG secondary antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
60.11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.9 mg/ml

Survivin Antibody (60.11) Summary

Immunogen
Full length recombinant human Survivin [UniProt# O15392]
Epitope
The epitope recognized is between amino acids 27-67
Localization
Cytoplasmic
Specificity
NB500-238 is specific for the cytoplasmic form of Survivin.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BIRC5
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA
  • Flow Cytometry 1 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Flow (Intracellular)
Application Notes
This Survivin (60.11) antibody is useful for Immunocytochemistry (PMID: 21976248) /Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot. By WB, this antibody recognizes a band at ~16.5 kDa representing Survivin. Flow Cytometry and ELISA were reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
16.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
Survivin Lysate (NBL1-07987)
Reviewed Applications
Read 2 Reviews rated 4
using
NB500-238 in the following applications:

Publications
Read Publications using
NB500-238 in the following applications:

Reactivity Notes

Human, rat and mouse.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
0.9 mg/ml
Purity
Protein G purified

Alternate Names for Survivin Antibody (60.11)

  • API4 survivin variant 3 alpha
  • API4
  • Apoptosis inhibitor 4 baculoviral IAP repeat-containing protein 5
  • Apoptosis inhibitor survivin
  • baculoviral IAP repeat-containing 5
  • BIRC5
  • EPR-1
  • IAP4
  • Survivin

Background

Survivin (BIRC5 or IAP4) is the smallest member of inhibitors of apoptosis proteins (X-linked IAP/XIAP, c-IAP1, c-IAP2, IAP-like protein-2/ ILP2, melanoma IAP/ ML-IAP, Livin, NAIP, and survivin) and besides its anti-apoptotic functions; survivin also regulates processes such as cell cycle, migration, metastasis etc. Highly critical in fetal development, survivin is generally no longer present in adult tissues; however, its re-expression occurs in cancers wherein it drives proliferation, metastasis, poor prognosis, and decreased patient survival. Survivin contains only one copy of a modified baculovirus IAP repeat (BIR, a domain required for caspases inactivation) which is used for homodimerization and for its interaction with other chromosome passenger proteins. Phosphorylation/mutation of Thr-48 in the BIR domain affects survivin's dual ability to inhibit apoptosis and regulate cell division. Under apoptotic stimuli involving mitochondria, survivin forms a complex with XIAP, thus increasing the stability of XIAP and its inhibitory activity against caspases. The survivin binding to mitochondrial Smac/DIABLO leads to a delayed Smac/DIABLO release in the cytoplasm resulting in prolonged cell survival. Survivin-HBXIP complex can bind to pro-caspase-9 for exerting prevention of mitochondrial apoptosis activation. For facilitating correct cell division, survivin recognize/complex with phospho-histone-H3 and associates with the chromosome passenger proteins (Aurora B, INCENP, and borealin) and this new complex is recruited to the mitotic centromers where it assists/ensure proper chromosomal segregation. Moreover, at the centrosomes of dividing cells, survivin binds to Cdk1 which is critical for cells to enter mitosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Survivin Antibody (NB500-238)(34)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: ELISA, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, PLA, WB.


Filter By Application
ELISA
(1)
FLOW
(2)
ICC/IF
(6)
IHC
(2)
IHC-Fr
(1)
IHC-P
(5)
PLA
(1)
WB
(14)
All Applications
Filter By Species
Human
(21)
Mouse
(4)
Rat
(1)
All Species
Showing Publications 1 - 10 of 34. Show All 34 Publications.
Publications using NB500-238 Applications Species
BAE I, KIM C, KIM J et al. Correlation of survivin and B-cell lymphoma 2 expression with pathological malignancy and anti-apoptotic properties of glial cell tumors. BIOMEDICAL REPORTS. Jan 27 2017 [PMID: 28413637] (WB, Human) WB Human
Dheekollu J, Malecka K, Wiedmer A et al. Carcinoma-risk variant of EBNA1 deregulates Epstein-Barr Virus episomal latency. Oncotarget. [PMID: 28077791] (PLA)

Details:
Citation using the Unpurified form of this antibody.
PLA
Valenzuela MMA, Castro I, Gonda A. Cell death in response to antimetabolites directed at ribonucleotide reductase and thymidylate synthase Onco Targets Ther 2015 Feb 26 [PMID: 25767396] (WB, Human) WB Human
Kusner LL, Ciesielski MJ, Marx A et al. Survivin as a potential mediator to support autoreactive cell survival in myasthenia gravis: a human and animal model study. PLoS ONE 2014 Jul 23 [PMID: 25050620] (FLOW, Human)

Details:
Using the DyLight 650 conjugated version of NB500-238, catalog number NB500-238C.
FLOW Human
Kapinas Kristina, Kim Heesun, Mandeville Matthew et al. microRNA-mediated survivin control of pluripotency. J Cell Physiol. [PMID: 24891298] (Human)

Details:
Citation using the Unpurified form of this antibody.
Human
Rho JH, Lampe PD. High-Throughput Screening for Native Autoantigen-Autoantibody Complexes Using Antibody Microarrays J Proteome Res 2013 Mar 29 [PMID: 23541305] (Human) Human
Khan S, Jutzy JM, Valenzuela MM et al. Plasma-derived exosomal survivin, a plausible biomarker for early detection of prostate cancer PLoS One 2012 [PMID: 23091600] (WB, Human) WB Human
Hoel AW, Yu P, Nguyen KP, et al. Mitochondrial Heat Shock Protein-90 Modulates Vascular Smooth Muscle Cell Survival and the Vascular Injury Response in Vivo. Am J Pathol. [PMID: 22841823] (WB, IHC-Fr, Human)

Details:
Citation using the Unpurified form of this antibody.
WB, IHC-Fr Human
de Souza HS, West GA, Rebert N et al. Increased Levels of Survivin, via Association With Heat Shock Protein 90, in Mucosal T Cells From Patients With Crohn's Disease. Gastroenterology. [PMID: 22749932] (Human)

Details:
Citation using the Unpurified form of this antibody.
Human
Zhang H, Wang Y, Liu C et al. DNA and adenovirus tumor vaccine expressing truncated survivin generates specific immune responses and anti-tumor effects in a murine melanoma model Cancer Immunol Immunother 2012 Jun 17 [PMID: 22706381] (WB, Human) WB Human
Show All 34 Publications.

Reviews for Survivin Antibody (NB500-238) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 review tested in 1 application: WB.

Reviews using NB500-238:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Survivin NB500-238
Enlarge
reviewed by:
Anonymous
WB Human 08/24/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman Breast Cancer Cell line SKBR3 cell lysates
SpeciesHuman
LotLot B
 
reviewed by:
Anonymous
WB Mouse 12/08/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse heart
SpeciesMouse

Blocking

Blocking DetailsBlocking Buffer: 5% BSA/TBST

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat anti-mouse

Details

Detection NotesDetection Method: ECL

Product General Protocols

View specific protocols for Survivin Antibody (NB500-238): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Survivin Antibody (NB500-238) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB500-238AF405
Alexa Fluor 488 Labeled NB500-238AF488
Alexa Fluor 647 Labeled NB500-238AF647
Alexa Fluor 700 Labeled NB500-238AF700
Allophycocyanin Labeled NB500-238APC
Biotin Labeled NB500-238B
DyLight 350 Labeled NB500-238UV
DyLight 405 Labeled NB500-238V
DyLight 488 Labeled NB500-238G
DyLight 550 Labeled NB500-238R
DyLight 650 Labeled NB500-238C
DyLight 680 Labeled NB500-238FR
DyLight 755 Labeled NB500-238IR
FITC Labeled NB500-238F
HRP Labeled NB500-238H
PE Labeled NB500-238PE
PerCP Labeled NB500-238PCP

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Blogs on Survivin. Showing 1-10 of 14 blog posts - Show all blog posts.

Survivin - an inhibitor of apoptosis that drives tumorigenesis and metastasis
Untitled DocumentApoptosis is the tightly regulated process of programmed cell death. It plays an important role in normal physiologic development but has also been implicated in a number of diseases. Apoptosis is constantly downreg...  Read full blog post.

Survivin - an inhibitor of apoptosis protein
Survivin is an anti-apoptotic protein which is the smallest protein within a large family of proteins including X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, NAIP, and Livin. Survivin is responsible for a wide range of basic cellula...  Read full blog post.

Cytochrome C - a mediator of apoptosis
Cytochrome C is a small heme protein within the inner mitochondrial membrane responsible for carrying electrons within the respiratory transport chain.  Additionally, cytochrome c has also been identified as a player in programmed cell death (apop...  Read full blog post.

PCNA (Proliferating cell nuclear antigen, polymerase delta auxiliary protein)
PCNA is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It coordinates the recruitment and association of needed components during both of these processes, both of which are essential for cell cycl...  Read full blog post.

Survivin is thrivin'
The survivin anti-apoptotic protein is the smallest member of a large family of proteins such as X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates basic physiological events such as the cell cycle, tumor...  Read full blog post.

Livin: On a Prayer
Livin is a member of the inhibitor of apoptosis proteins (IAP) family that regulates programmed cell death. The Livin protein contains a single baculovirus IAP repeat (BIR) essential for function, along with a COOH-terminal RING-type zinc finger domai...  Read full blog post.

Survivin: As long as I know how to live I know I will
Survivin is an anti-apoptotic protein from a large family with related members such as X-linked IAP, cIAP1 and cIAP2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates fundamental physiological events including the cell cycle, feta...  Read full blog post.

Survivin: Infographic
Survivin is involved in promoting cell proliferation and is an inhibitor of apoptosis. Survivin has a critical role in cancer proliferation and neural development. It may have an impact on neural cell proliferative responses following brain injury.L...  Read full blog post.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its...  Read full blog post.

Caspase 7: The Cell's Suicide Switch
Caspase 7 (also known as CASP7, Mch3, ICE-LAP3, CMH-1) is a member of caspase family of cysteine proteases. It is an apoptosis-related cystein peptidase encoded by the CASP7 gene in humans. CASP7 homologous sequences have been identified in nearly all...  Read full blog post.

Showing 1-10 of 14 blog posts - Show all blog posts.

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Anonymous
08/24/2017
Application: WB
Species: Human

 
Anonymous
12/08/2011
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol BIRC5
Entrez