Survivin Antibody (32.1)

Images

 
Immunohistochemistry-Paraffin: Survivin Antibody (32.1) [NB500-237] - Immunohistochemical staining of formalin-fixed paraffin-embedded human lung cancer tissue using Survivin Antibody (32.1) [NB500-237].
Flow Cytometry: Survivin Antibody (32.1) [NB500-237] - An intracellular stain was performed on HeLa cells with FITC-conjugated Survivin Antibody (32.1) [NB500-237F] (blue) and a matched isotype control (orange). Cells ...read more

Product Details

Summary
Reactivity Hu, HaSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
Clone
32.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.9 mg/ml

Survivin Antibody (32.1) Summary

Immunogen
This Survivin Antibody (32.1) was developed against full length recombinant human Survivin [UniProt# O15392]
Epitope
The epitope recognized is between amino acids 3-19
Localization
Nuclear
Specificity
Survivin Antibody (32.1) [NB500-237] is specific for the nuclear form of survivin.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BIRC5
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 20 ug/ml
  • Immunohistochemistry-Paraffin 20 ug/ml
  • Immunoprecipitation
Application Notes
Use in ELISA and Immunoprecipitation reported in scientific literature (PMID: 11861764). By WB, this antibody recognizes a band at ~16.5 kDa representing Survivin. *The investigator should determine the optimal working dilution for a specific application.
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Nuclear Cell Lysate
Survivin Overexpression Lysate
Publications
Read Publications using
NB500-237 in the following applications:

  • 1 publication
  • 2 publications
  • 3 publications
  • IHC
    1 publication
  • 2 publications
  • IP
    2 publications
  • WB
    3 publications

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 23405201).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.9 mg/ml
Purity
Protein G purified

Alternate Names for Survivin Antibody (32.1)

  • API4
  • apoptosis inhibitor 4
  • Apoptosis inhibitor survivin
  • baculoviral IAP repeat-containing 5
  • Baculoviral IAP Repeat-Containing Protein 5
  • BIRC5
  • EPR-1
  • IAP4
  • survivin variant 3 alpha
  • Survivin

Background

Survivin (BIRC5 or IAP4) is the smallest member of the inhibitor of apoptosis (IAP) proteins, containing only a single baculovirus IAP repeat (BIR) domain and lacking the C-terminal RING domain. Survivin has a theoretical molecular weight of 16.5 kDa (wild-type protein) and exists as ten splice variant forms including the most predominant isoforms, survivin-2B and surviving-DeltaEx3 (1). It also undergoes phosphorylation by PKA, Plk1, Cdk1, CKII and Aurora B at multiple sites (e.g. Serine 20 and Threonine 34, 53 and 117).

Besides being highly abundant in fetal development and expressed in proliferating adult cells such as activated T lymphocytes, erythroblasts, and self-renewing stem cells, survivin is generally absent in adult tissues. However, it is elevated in common cancers such as lung, colon, pancreas, breast and prostate where it drives proliferation, metastasis, poor prognosis, and decreased patient survival (2).

Survivin has been shown to be involved in multiple cellular processes including cell cycle progression, mitotic spindle assembly, kinetochore attachment, angiogenesis, migration, and its anti-apoptotic activity has been linked to both its monomeric and homodimeric forms. Survivin impacts the function of other IAP members, c-IAP1 and c-IAP-2, or modulates the inhibitory activity of XIAP against caspases by forming a stable complex with XIAP and HBXIP. During the intrinsic apoptotic pathway, survivin may prevent the release of mitochondrial APAF1 into the cytoplasm or hinder the association of SMAC with other IAPS, which results in prolonged cell survival (3).

References

1. Sah NK, Seniya C. (2015) Survivin splice variants and their diagnostic significance. Tumour Biol. 36(9):6623-31. PMID: 26245993

2. Lladser A, Sanhueza C, Kiessling R, Quest AF. (2011) Is survivin the potential Achilles' heel of cancer? Adv Cancer Res. 111:1-37. PMID: 21704829

3. Wheatley SP, Altieri DC. (2019) Survivin at a glance. J Cell Sci. 132(7). PMID: 30948431

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Survivin Antibody (NB500-237)(12)

We have publications tested in 2 confirmed species: Human, Hamster.

We have publications tested in 7 applications: ELISA, Flow, ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ELISA
(1)
Flow
(2)
ICC/IF
(3)
IHC
(1)
IHC-P
(2)
IP
(2)
WB
(3)
All Applications
Filter By Species
Human
(9)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB500-237 Applications Species
Hori M, Miki T, Okamoto M et al. The Detergent-Soluble Cytoplasmic Pool of Survivin Suppresses Anoikis and Its Expression Is Associated with Metastatic Disease of Human Colon Cancer. PLoS One 2013 [PMID: 23405201] (WB, Human, Hamster) WB Human, Hamster
Guvenc H, Pavlyukov MS, Joshi K et al. Impairment of Glioma Stem Cell Survival and Growth by a Novel Inhibitor for Survivin-Ran Protein Complex. Clin Cancer Res 2013 Jan 18 [PMID: 23251006] (IP, Human) IP Human
Lechler P, Schaumburger J, Kock FX et al. The Oncofetal Gene Survivin Promotes Cell Proliferation and Survival in Primary Human Osteoblastic Cells. Calcif Tissue Int. 2011 Jun 15. [PMID: 21674243] (ICC/IF, Human) ICC/IF Human
Lechler P, Renkawitz T, Campean V et al. The antiapoptotic gene survivin is highly expressed in human chondrosarcoma and promotes drug resistance in chondrosarcoma cells in vitro. BMC Cancer11(1):120. 2011 Apr 2. [PMID: 21457573]
Qi G, Tuncel H, Aoki E et al. Intracellular localization of survivin determines biological behavior in colorectal cancer. Oncol Rep 2009 Sep [PMID: 19639203] (IHC, WB, Human) IHC, WB Human
Lechler P, Wu X, Bernhardt W, Campean V, Gastiger S, Hackenbeck T, Klanke B, Weidemann A, Warnecke C, Amann K, Engehausen D, Willam C, Eckardt KU, Rodel F, Wiesener MS. The tumor gene survivin is highly expressed in adult renal tubular cells: implications for a pathophysiological role in the kidney. Am J Pathol171(5):1483-98. 2007 Nov. [PMID: 17982126] (IHC-P) IHC-P
Ding Y, Prieto VG, Zhang PS, Rosenthal S, Smith KJ, Skelton HG, Diwan AH. Nuclear expression of the antiapoptotic protein survivin in malignant melanoma. Cancer106(5):1123-9. 2006 Mar 1. [PMID: 16456815] (IHC-P, Human) IHC-P Human
Giodini, A et al. Regulation of microtubule stability and mitotic progression by survivin. Cancer Res62(9):2462-7. 2002 May 1. [PMID: 11980633]
Fortugno, P et al. Survivin exists in immunochemically distinct subcellular pools and is involved in spindle microtubule function. J Cell Sci115(Pt 3):575-85. 2002 Feb 1. [PMID: 11861764] (WB, IP, ELISA, ICC/IF, Human) WB, IP, ELISA, ICC/IF Human
Wu X, Li Y, Liu X et al. B7-H1(PD-L1) confers chemoresistance through ERK and p38 MAPK pathway in tumor cells bioRxiv 2018 Apr 25 (Flow, Human) Flow Human
Show All 12 Publications.

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Product General Protocols

View specific protocols for Survivin Antibody (NB500-237): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Survivin Antibody (NB500-237). (Showing 1 - 2 of 2 FAQs).

  1. Can I use this antibody with species other than those listed?
    • The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.
  2. Where is the cellular localization of this antibody?
    • The localization of this survivin antibody is nuclear.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB500-237AF350
Alexa Fluor 405 NB500-237AF405
Alexa Fluor 488 NB500-237AF488
Alexa Fluor 532 NB500-237AF532
Alexa Fluor 594 NB500-237AF594
Alexa Fluor 647 NB500-237AF647
Alexa Fluor 700 NB500-237AF700
Alexa Fluor 750 NB500-237AF750
Biotin NB500-237B
DyLight 350 NB500-237UV
DyLight 405 NB500-237V
DyLight 488 NB500-237G
DyLight 550 NB500-237R
DyLight 594 NB500-237DL594
DyLight 650 NB500-237C
DyLight 680 NB500-237FR
DyLight 755 NB500-237IR
FITC NB500-237F
HRP NB500-237H
Janelia Fluor 549 NB500-237JF549
Janelia Fluor 646 NB500-237JF646

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Bioinformatics Tool for Survivin Antibody (NB500-237)

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Blogs on Survivin. Showing 1-10 of 15 blog posts - Show all blog posts.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of...  Read full blog post.

Survivin - an inhibitor of apoptosis that drives tumorigenesis and metastasis
Apoptosis is the tightly regulated process of programmed cell death. It plays an important role in normal physiologic development but has also been implicated in a number of diseases. Apoptosis is constantly downregulated by a family of anti-apop...  Read full blog post.

Survivin - an inhibitor of apoptosis protein
Survivin is an anti-apoptotic protein which is the smallest protein within a large family of proteins including X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, NAIP, and Livin. Survivin is responsible for a wide range of basic cellula...  Read full blog post.

Cytochrome C - a mediator of apoptosis
Cytochrome C is a small heme protein within the inner mitochondrial membrane responsible for carrying electrons within the respiratory transport chain.  Additionally, cytochrome c has also been identified as a player in programmed cell death (apop...  Read full blog post.

PCNA (Proliferating cell nuclear antigen, polymerase delta auxiliary protein)
PCNA is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It coordinates the recruitment and association of needed components during both of these processes, both of which are essential for cell cycl...  Read full blog post.

Survivin is thrivin'
The survivin anti-apoptotic protein is the smallest member of a large family of proteins such as X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates basic physiological events such as the cell cycle, tumor...  Read full blog post.

Livin: On a Prayer
Livin is a member of the inhibitor of apoptosis proteins (IAP) family that regulates programmed cell death. The Livin protein contains a single baculovirus IAP repeat (BIR) essential for function, along with a COOH-terminal RING-type zinc finger domai...  Read full blog post.

Survivin: As long as I know how to live I know I will
Survivin is an anti-apoptotic protein from a large family with related members such as X-linked IAP, cIAP1 and cIAP2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates fundamental physiological events including the cell cycle, feta...  Read full blog post.

Survivin: Infographic
Survivin is involved in promoting cell proliferation and is an inhibitor of apoptosis. Survivin has a critical role in cancer proliferation and neural development. It may have an impact on neural cell proliferative responses following brain injury.L...  Read full blog post.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its...  Read full blog post.

Showing 1-10 of 15 blog posts - Show all blog posts.
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Bioinformatics

Gene Symbol BIRC5