Nbs1 Antibody

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Western Blot: Nbs1 Antibody [NB100-60654] - Detection of Human Nbs1 on HeLa whole cell lysate using NB100-60654. Nbs1 was also immunoprecipitated by rabbit anti-NBS1 antibodies NB100-60653 and another Nbs1.
Immunohistochemistry: Nbs1 Antibody [NB100-60654] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NBS1 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Proximity Ligation Assay: Nbs1 Antibody [NB100-60654] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human DMAP1 (NB100-256) and rabbit anti-human NBS1 (NB100-60654). Image merged from DAPI (2ms) and ...read more
Proximity Ligation Assay: Nbs1 Antibody [NB100-60654] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human NBS1 (NB100-60654). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: Nbs1 Antibody [NB100-60654] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human NBS1 (NB100-60654). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: Nbs1 Antibody [NB100-60654] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human HP1-beta (NB100-79774) and rabbit anti-human NBS1 (NB100-60654). Image merged from DAPI (2ms) and ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

Nbs1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 704 and 754 of human Nibrin using the numbering given in entry NP_002476.2 (GeneID 4683).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NBN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg
  • Proximity Ligation Assay 1:200-1:2000
Application Notes
Use in Chromatin Immunoprecipitation reported in scientific literature (PMID: 23852118) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Nbs1 Lysate (NBL1-13497)
Publications
Read Publications using
NB100-60654 in the following applications:

  • 2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23852118). Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, Primates, Crab-eating macaque, Olive baboon, Agile gibbon, Siamang, BorneanBorangutan, Black-and-white colobus monkey, Angolan talapoin and Northern white-cheeked gibbon.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nbs1 Antibody

  • ATV
  • AT-V1
  • AT-V2
  • Cell cycle regulatory protein p95
  • FLJ10155
  • MGC87362
  • NBN
  • Nbs1
  • NBS1P95
  • NBSNijmegen breakage syndrome protein 1
  • Nibrin
  • Nijmegen breakage syndrome 1 (nibrin)
  • p95 protein of the MRE11/RAD50 complex
  • p95

Background

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ChIP, IHC-P, IP, PLA

Publications for Nbs1 Antibody (NB100-60654)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: ChIP.


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ChIP
(2)
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(1)
Mouse
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for Nbs1 Antibody (NB100-60654) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Nbs1 Antibody (NB100-60654)

Discover related pathways, diseases and genes to Nbs1 Antibody (NB100-60654). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Nbs1 Antibody (NB100-60654)

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Pathways for Nbs1 Antibody (NB100-60654)

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PTMs for Nbs1 Antibody (NB100-60654)

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Blogs on Nbs1.

The MRE11 Complex and DNA Damage Response
The maintenance of genome stability depends on the DNA damage response (DDR) which is a complex signaling network including cell cycle checkpoints, DNA repair and damage tolerance pathways. The DDR complex has the ability to sense DNA damage and tra...  Read full blog post.

NBS1: The DNA Repair Trigger
NBS1 (Nijmegen breakage syndrome protein 1) is a component of the MRN complex (Mre11-Rad50-Nbs1) that plays important role in detecting DNA double strand breaks (DSBs) and triggering the downstream cascade. DSBs can be caused by ionizing radiation, ch...  Read full blog post.

NBS1: DNA Repair Trigger
NBS1 (Nijmegen breakage syndrome protein 1) is a component of the MRN complex (Mre11-Rad50-Nbs1) that plays important role in detecting DNA double strand breaks (DSBs) and triggering the downstream cascade. DSBs can be caused by ionizing radiation, ch...  Read full blog post.

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Bioinformatics

Gene Symbol NBN
Entrez
Uniprot