ATM Antibody (2C1)

Images

 
Western Blot: ATM Antibody (2C1) [NB100-309] - Detection of human ATM protein using anti-ATM 2C1 monoclonal antibody by western blot or immunoprecipitation.
Immunohistochemistry-Paraffin: ATM Antibody (2C1) [NB100-309] - Human Kidney (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline ...read more
Western Blot: ATM Antibody (2C1) [NB100-309] - A. 30 ug NT2D1 whole cell lysate/extract, B: 30 ug PC-3 whole cell lysate/extract, C: 30 ug SK-NSh whole cell lysate/extract 5% SDS-PAGE, antibody dilution 1:1000.
Western Blot: ATM Antibody (2C1) [NB100-309] - analysis of ATM in U2OS sarcoma cells using anti-ATM antibody. Image from verified customer review.
Western Blot: ATM Antibody (2C1) [NB100-309] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] diluted at 1:1000.
Western Blot: ATM Antibody (2C1) [NB100-309] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] diluted at 1:1000.
Western Blot: ATM Antibody (2C1) [NB100-309] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] diluted at ...read more
Immunohistochemistry-Paraffin: ATM Antibody (2C1) [NB100-309] - Human Testis (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
Clone
2C1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.85 mg/ml
     

Genetic Strategies

   

ATM Antibody (2C1) Summary

Immunogen
Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056.
Localization
Nucleus. Cytoplasmic vesicle.
Specificity
NB100-309 recognizes full-length ATM, a 350-kDa nuclear phosphoprotein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ATM
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • ELISA 1:100 - 1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Immunoprecipitation 1-10 ug/ml
Application Notes
IHC-R. ICC/IF usage reported in scientifc literature (Harry Scherthan, et.al., 2000 and Yiyoung Liu, et.al., 2006). ELISA usage reported in scientific literature. WB: Predicted molecular weight: 350 kDa.
Theoretical MW
370 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-309 in the following applications:

Publications
Read Publications using
NB100-309 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Concentration
1.85 mg/ml
Purity
Protein G purified

Alternate Names for ATM Antibody (2C1)

  • AT mutated
  • A-T mutated
  • AT1
  • ATA
  • ataxia telangiectasia mutated (includes complementation groups A, C and D)
  • ataxia telangiectasia mutatedATD
  • ATC
  • ATDC
  • ATE
  • ATM
  • DKFZp781A0353
  • EC 2.7.11.1
  • MGC74674
  • serine-protein kinase ATM
  • TEL1
  • TEL1, telomere maintenance 1, homolog
  • TELO1
  • TPLL

Background

Ataxia-telangiectasia (A-T) is a recessive childhood disease caused by mutations in the ATM (AT-mutated) gene. Symptoms include neurological abnormalities that cause unsteady posture, dilated blood vessels, infertility, radiation sensitivity, immune deficiencies and lymphoid malignancies. It appears that the diverse defects seen in ATM null mammals are manifestations of disparate signal transduction defects. The ATM protein is related to a family of proteins through a c-terminal phoshatidylinositol 3-kinase (PI3-kinase) domain. Members of the PI3-kinase family of proteins are involved in cell cycle control, DNA replication, recombination and repair. ATM also shares sequence homology with portions of the yeast RAD3 gene. The main role of ATM appears to be induction of a DNA-damage control pathway in response to genotoxic insults, such as ionizing radiation or anti-tumor medications and the programmed DNA breaks of meiosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-30027
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-308
Species: Hu
Applications: WB, Func, ICC/IF, IP
NBP2-03417
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NB100-56585
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56546
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB100-384
Species: Hu, Mu, Rt, Ca
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
NB100-464
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, PLA
NB100-143
Species: Hu, Mu, Ha, Ma, Pm
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-P, IP, KO
NBP1-77368
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
AF1513
Species: Mu
Applications: WB, Simple Western, Flow, IHC, IP, CyTOF-ready
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-142
Species: Hu, Mu, Rt, Ch, Ha
Applications: WB, Simple Western, ChIP, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-22128
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC-P
NB100-598
Species: Hu
Applications: WB, Flow, ICC/IF, IP, CyTOF-ready
NB100-56155
Species: Hu
Applications: WB, IHC, IHC-P, IP
NB100-304
Species: Hu, Mu, Rt, Fi, Gt, Pm
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO
AF4090
Species: Hu
Applications: WB, IHC, IP, Neut

Publications for ATM Antibody (NB100-309)(114)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ELISA, FLOW, ICC/IF, IHC-P, IP, WB.


Filter By Application
ELISA
(4)
FLOW
(1)
ICC/IF
(6)
IHC-P
(1)
IP
(7)
WB
(68)
All Applications
Filter By Species
Human
(72)
Mouse
(4)
All Species
Showing Publications 1 - 10 of 114. Show All 114 Publications.
Publications using NB100-309 Applications Species
Park SJ, Kong HK, Kim YS et al. Inhibition of S-adenosylhomocysteine hydrolase decreases cell mobility and cell proliferation through cell cycle arrest. Am J Cancer Res 2015 [PMID: 26328244] (WB) WB
Muraki K, Han L, Miller D, Murnane JP et al. Processing by MRE11 is involved in the sensitivity of subtelomeric regions to DNA double-strand breaks Nucleic Acids Res. 2015 Jul 23 [PMID: 26209132] (WB, Human) WB Human
Simoes M, Martins C, Ferreira F et al. Early intranuclear replication of African swine fever virus genome modifies the landscape of the host cell nucleus Virus Res. 2015 Jul 13 [PMID: 26183880]
Vermezovic J, Adamowicz M, Santarpia L et al. Notch is a direct negative regulator of the DNA-damage response Nat. Struct. Mol. Biol. 2015 Apr 20 [PMID: 25895060] (IHC-P, WB, Human) IHC-P, WB Human
Batenburg NL, Thompson EL, Hendrickson EA, Zhu XD. Cockayne syndrome group B protein regulates DNA double-strand break repair and checkpoint activation. EMBO J. 2015 Mar 27 [PMID: 25820262] (WB, Mouse) WB Mouse
Min W, Bruhn C, Grigaravicius et al. Poly(ADP-ribose) binding to Chk1 at stalled replication forks is required for S-phase checkpoint activation. Nat Commun 2013 Dec 20 [PMID: 24356582] (WB, Mouse) WB Mouse
Simoes M, Martins C, Ferreira F. Host DNA Damage Response facilitates African Swine Fever Virus infection. Vet Microbiol 2013 Jan 23 [PMID: 23398667]
Gutierrez-Gonzalez A, Belda-Iniesta C, Bargiela-Iparraguirre J et al. Targeting Chk2 improves gastric cancer chemotherapy by impairing DNA damage repair Apoptosis 2012 Dec 28 [PMID: 23271172] (WB, Human) WB Human
Shi Y, Felley-Bosco E, Marti TM et al. Starvation-induced activation of ATM/Chk2/p53 signaling sensitizes cancer cells to cisplatin. BMC Cancer 2012 Dec [PMID: 23211021] (WB, Human) WB Human
Murad NA, Cullen JK, McKenzie M et al. Mitochondrial dysfunction in a novel form of autosomal recessive ataxia. Mitochondrion 2012 Nov [PMID: 23178371] (WB, Human) WB Human
Show All 114 Publications.

Reviews for ATM Antibody (NB100-309) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 1 application: WB.

Reviews using NB100-309:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot ATM NB100-309
Enlarge
reviewed by:
Anonymous
WB Human 04/28/2015
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsU2OS sarcoma cells
FileView PDF
 
reviewed by:
Takeshi Shi
WB Human 11/02/2014
View

Summary

ApplicationWestern Blot
Sample TestedU2OS whole cell lysate
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for ATM Antibody (NB100-309) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-309AF405
Alexa Fluor 488 Labeled NB100-309AF488
Alexa Fluor 647 Labeled NB100-309AF647
Alexa Fluor 700 Labeled NB100-309AF700
Allophycocyanin Labeled NB100-309APC
DyLight 350 Labeled NB100-309UV
DyLight 405 Labeled NB100-309V
DyLight 488 Labeled NB100-309G
DyLight 680 Labeled NB100-309FR
DyLight 755 Labeled NB100-309IR
PE Labeled NB100-309PE

Additional ATM Products

Bioinformatics Tool for ATM Antibody (NB100-309)

Discover related pathways, diseases and genes to ATM Antibody (NB100-309). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ATM Antibody (NB100-309)

Discover more about diseases related to ATM Antibody (NB100-309).
 

Pathways for ATM Antibody (NB100-309)

View related products by pathway.

PTMs for ATM Antibody (NB100-309)

Learn more about PTMs related to ATM Antibody (NB100-309).
 

Research Areas for ATM Antibody (NB100-309)

Find related products by research area.

Blogs on ATM.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels...  Read full blog post.

53BP1 - a marker for DNA Double Strand Break
53BP1 (p53 binding protein 1) was originally thought to be an enhancer for p53 transcriptional, but later studies have demonstrated that it is actually a substrate for ataxia telangiectasia mutated (ATM). 53BP1 is a classic late DNA damage response...  Read full blog post.

53BP1 - DNA damage is no fun
The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri...  Read full blog post.

ATM and DSB Repair in Cancer
Ataxia Telangiectasia Mutated (ATM) is a serine/threonine protein kinase that is the master regulator of the DNA double-strand break (DSB) repair pathway. ATM is a key part of the cell cycle machinery that activates checkpoint signaling in response to...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Anonymous
04/28/2015
Application: WB
Species: Human

 
Takeshi Shi
11/02/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol ATM
Entrez
Uniprot