ATM Antibody (2C1)


Western Blot: ATM Antibody (2C1) [NB100-309] - Detection of human ATM protein using anti-ATM 2C1 monoclonal antibody by western blot or immunoprecipitation.
Immunohistochemistry-Paraffin: ATM Antibody (2C1) [NB100-309] - Human Kidney (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline more
Western Blot: ATM Antibody (2C1) [NB100-309] - analysis of ATM in U2OS sarcoma cells using anti-ATM antibody. Image from verified customer review.
Western Blot: ATM Antibody (2C1) [NB100-309] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] diluted at 1:1000.
Western Blot: ATM Antibody (2C1) [NB100-309] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] diluted at 1:1000.
Genetic Strategies: Western Blot: ATM Antibody (2C1) [NB100-309] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM more
Immunohistochemistry-Paraffin: ATM Antibody (2C1) [NB100-309] - Human Testis (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline more

Product Details

Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
Validated by:

Genetic Strategies


ATM Antibody (2C1) Summary

Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056.
Nucleus. Cytoplasmic vesicle.
NB100-309 recognizes full-length ATM, a 350-kDa nuclear phosphoprotein.
Protein G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:500-1:3000
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • ELISA 1:100 - 1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Immunoprecipitation 1-10 ug/ml
Application Notes
IHC-R. ICC/IF usage reported in scientifc literature (Harry Scherthan,, 2000 and Yiyoung Liu,, 2006). ELISA usage reported in scientific literature. WB: Predicted molecular weight: 350 kDa.
Theoretical MW
370 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
NB100-309 in the following applications:

Read Publications using
NB100-309 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
No Preservative
Protein G purified

Alternate Names for ATM Antibody (2C1)

  • AT mutated
  • A-T mutated
  • AT1
  • ATA
  • ataxia telangiectasia mutated (includes complementation groups A, C and D)
  • ataxia telangiectasia mutatedATD
  • ATC
  • ATDC
  • ATE
  • ATM
  • DKFZp781A0353
  • EC
  • MGC74674
  • serine-protein kinase ATM
  • TEL1
  • TEL1, telomere maintenance 1, homolog
  • TELO1
  • TPLL


Ataxia-telangiectasia (A-T) is a recessive childhood disease caused by mutations in the ATM (AT-mutated) gene. Symptoms include neurological abnormalities that cause unsteady posture, dilated blood vessels, infertility, radiation sensitivity, immune deficiencies and lymphoid malignancies. It appears that the diverse defects seen in ATM null mammals are manifestations of disparate signal transduction defects. The ATM protein is related to a family of proteins through a c-terminal phoshatidylinositol 3-kinase (PI3-kinase) domain. Members of the PI3-kinase family of proteins are involved in cell cycle control, DNA replication, recombination and repair. ATM also shares sequence homology with portions of the yeast RAD3 gene. The main role of ATM appears to be induction of a DNA-damage control pathway in response to genotoxic insults, such as ionizing radiation or anti-tumor medications and the programmed DNA breaks of meiosis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATM Antibody (NB100-309)(116)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ELISA, FLOW, ICC/IF, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 116. Show All 116 Publications.
Publications using NB100-309 Applications Species
Goder A, Emmerich C, Nikolova T, Kiweler N. HDAC1 and HDAC2 integrate checkpoint kinase phosphorylation and cell fate through the phosphatase-2A subunit PR130. Nat Commun. 2018 Feb 22 [PMID: 29472538] (WB) WB
Fu X, Zhang C, Meng H, Zhang K. Oncoprotein Tudor-SN is a key determinant providing survival advantage under DNA damaging stress. Cell Death Differ. 2018 Feb 19 [PMID: 29459768] (WB) WB
Park SJ, Kong HK, Kim YS et al. Inhibition of S-adenosylhomocysteine hydrolase decreases cell mobility and cell proliferation through cell cycle arrest. Am J Cancer Res 2015 [PMID: 26328244] (WB) WB
Muraki K, Han L, Miller D, Murnane JP et al. Processing by MRE11 is involved in the sensitivity of subtelomeric regions to DNA double-strand breaks Nucleic Acids Res. 2015 Jul 23 [PMID: 26209132] (WB, Human) WB Human
Simoes M, Martins C, Ferreira F et al. Early intranuclear replication of African swine fever virus genome modifies the landscape of the host cell nucleus Virus Res. 2015 Jul 13 [PMID: 26183880]
Vermezovic J, Adamowicz M, Santarpia L et al. Notch is a direct negative regulator of the DNA-damage response Nat. Struct. Mol. Biol. 2015 Apr 20 [PMID: 25895060] (IHC-P, WB, Human) IHC-P, WB Human
Batenburg NL, Thompson EL, Hendrickson EA, Zhu XD. Cockayne syndrome group B protein regulates DNA double-strand break repair and checkpoint activation. EMBO J. 2015 Mar 27 [PMID: 25820262] (WB, Mouse) WB Mouse
Min W, Bruhn C, Grigaravicius et al. Poly(ADP-ribose) binding to Chk1 at stalled replication forks is required for S-phase checkpoint activation. Nat Commun 2013 Dec 20 [PMID: 24356582] (WB, Mouse) WB Mouse
Simoes M, Martins C, Ferreira F. Host DNA Damage Response facilitates African Swine Fever Virus infection. Vet Microbiol 2013 Jan 23 [PMID: 23398667]
Gutierrez-Gonzalez A, Belda-Iniesta C, Bargiela-Iparraguirre J et al. Targeting Chk2 improves gastric cancer chemotherapy by impairing DNA damage repair Apoptosis 2012 Dec 28 [PMID: 23271172] (WB, Human) WB Human
Show All 116 Publications.

Reviews for ATM Antibody (NB100-309) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB100-309:
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Images Ratings Applications Species Date Details
Western Blot ATM NB100-309
reviewed by:
WB Human 04/28/2015


ApplicationWestern Blot
Special ApplicationsU2OS sarcoma cells
FileView PDF
reviewed by:
Takeshi Shi
WB Human 11/02/2014


ApplicationWestern Blot
Sample TestedU2OS whole cell lysate

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for ATM Antibody (NB100-309) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-309AF405
Alexa Fluor 488 Labeled NB100-309AF488
Alexa Fluor 647 Labeled NB100-309AF647
Alexa Fluor 700 Labeled NB100-309AF700
Allophycocyanin Labeled NB100-309APC
DyLight 350 Labeled NB100-309UV
DyLight 405 Labeled NB100-309V
DyLight 488 Labeled NB100-309G
DyLight 680 Labeled NB100-309FR
DyLight 755 Labeled NB100-309IR
PE Labeled NB100-309PE

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Blogs on ATM.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels...  Read full blog post.

53BP1 - a marker for DNA Double Strand Break
53BP1 (p53 binding protein 1) was originally thought to be an enhancer for p53 transcriptional, but later studies have demonstrated that it is actually a substrate for ataxia telangiectasia mutated (ATM). 53BP1 is a classic late DNA damage response...  Read full blog post.

53BP1 - DNA damage is no fun
The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri...  Read full blog post.

ATM and DSB Repair in Cancer
Ataxia Telangiectasia Mutated (ATM) is a serine/threonine protein kinase that is the master regulator of the DNA double-strand break (DSB) repair pathway. ATM is a key part of the cell cycle machinery that activates checkpoint signaling in response to...  Read full blog post.

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Recent Reviews


Application: WB
Species: Human

Takeshi Shi
Application: WB
Species: Human


Gene Symbol ATM