Rad51 Antibody (14B4)

Images

 
Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (70 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - staining of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody. Cells were pre-extracted with CSK buffer before fixation with 4% PFA. ...read more
Immunohistochemistry-Paraffin: Rad51 Antibody (14B4) [NB100-148] - BT483 xenograft. RAD51 antibody 14B4 dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Rad51 Antibody (14B4) [NB100-148] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The signal was developed with Trident ...read more
Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated ...read more
Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated ...read more
Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated ...read more
Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated ...read more
Immunoprecipitation: Rad51 Antibody (14B4) [NB100-148] - HeLa cell extract prepared with lysis 180 buffer (40 mM Tris-HCl pH8.0, 180 mM NaCl, 1 mMEDTA, 0.5% NP-40). Rabbit anti-RAD51 antibody was used for subsequent WB ...read more
Genetic Strategies: Knockdown Validated: Rad51 Antibody (14B4) [NB100-148] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ce, ChSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, IP, PLA, KD
Clone
14B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Rad51 Antibody (14B4) Summary

Immunogen
Full length (amino acids 1-338) Rad51 expressed in E. coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
RAD51
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
  • Immunoprecipitation
  • In vitro assay
  • Proximity Ligation Assay
  • Knockdown Validated
Application Notes
ChIP usage reported in scientific literature (PMID: 24023853). Rad51 antibody validated for IHC-F from a verified customer review. Use in In vitro reported in scientific literature (PMID 27815389).
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Bladder Whole Tissue Lysate (Adult Whole Tumor)
Rad51 Overexpression Lysate
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB100-148 in the following applications:

Publications
Read Publications using
NB100-148 in the following applications:

Reactivity Notes

C.elegans reactivity reported in scientific literature (PMID: 23942865). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Antigen Affinity-purified

Alternate Names for Rad51 Antibody (14B4)

  • BRCC5
  • hRAD51
  • HsRAD51
  • HsT16930
  • RAD51 (S. cerevisiae) homolog (E coli RecA homolog)
  • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
  • RAD51 homolog A
  • Rad51
  • RAD51ABRCA1/BRCA2-containing complex, subunit 5
  • RecA, E. coli, homolog of
  • RECADNA repair protein RAD51 homolog 1
  • RecA-like protein
  • recombination protein A

Background

Rad51, a 37kDa protein, is the human homolog of E. coli RecA protein and a member of the RAD52 epistasis group in S. cerevisiae. In recent studies it has been reported that BRCA1 interacts with Rad51 and disease-causing mutations have been found in the BRCA1 region necessary for BRCA1/Rad51 interaction, implying that this interaction is important for tumor suppression. BRCA2 and Rad51 have also been shown to interact by direct binding of the BRC repeats located within exon 11 of BRCA2. When these repeats are deleted, the interaction is lost and cells become hypersensitive to the DNA damage caused by methyl methanesulfonate (MMS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rad51 Antibody (NB100-148)(44)

We have publications tested in 3 confirmed species: Human, Mouse, C. elegans.

We have publications tested in 5 applications: ChIP, ICC/IF, IHC, In vitro, WB.


Filter By Application
ChIP
(1)
ICC/IF
(19)
IHC
(5)
In vitro
(1)
WB
(6)
All Applications
Filter By Species
Human
(12)
Mouse
(1)
C. elegans
(6)
All Species
Showing Publications 1 - 10 of 44. Show All 44 Publications.
Publications using NB100-148 Applications Species
Tu Z, Mu X, Chen X, et al. Dibutyl phthalate exposure disrupts the progression of meiotic prophase I by interfering with homologous recombination in fetal mouse oocytes Environ. Pollut. May 24 2019 [PMID: 31158667] (ICC/IF, Mouse) ICC/IF Mouse
Deveryshetty, J;Peterlini, T;Ryzhikov, M;Brahiti, N;Dellaire, G;Masson, JY;Korolev, S; Novel RNA and DNA strand exchange activity of the PALB2 DNA Binding Domain and its critical role for DNA repair in cells Elife Apr 24 2019 [PMID: 31017574] (WB, Human) WB Human
Shin N, Cuenca L, Karthikraj R et al. Assessing effects of germline exposure to environmental toxicants by high-throughput screening in C. elegans PLoS Genet. Feb 1 2019 [PMID: 30763314] (ICC/IF, C. elegans) ICC/IF C. elegans
Garcia-Muse T, Galindo-Diaz U, Garcia-Rubio M et al. A Meiotic Checkpoint Alters Repair Partner Bias to Permit Inter-sister Repair of Persistent DSBs. Cell Rep Jan 15 2019 [PMID: 30650366] (IHC, C. elegans) IHC C. elegans
Sulkowski PL, Sundaram RK, Oeck S et al. Krebs-cycle-deficient hereditary cancer syndromes are defined by defects in homologous-recombination DNA repair. Nat. Genet. Aug 1 2018 [PMID: 30013182] (WB, Human) WB Human
PeriaSamy J, Kurdekar V, Jasti S et al. Targeting Phosphopeptide Recognition by the Human BRCA1 Tandem BRCT Domain to Interrupt BRCA1-Dependent Signaling. Cell Chem Biol Mar 9 2018 [PMID: 29606576] (ICC/IF) ICC/IF
Scanlon SE, Hegan DC, Sulkowski PL, Glazer PM. Suppression of homology-dependent DNA double-strand break repair induces PARP inhibitor sensitivity inVHL-deficient human renal cell carcinoma. Oncotarget. 2018 Jan 12 [PMID: 29435132] (WB, Human) WB Human
Li Q, Xie W, Wang N et al. CDC7-dependent transcriptional regulation of RAD54L is essential for tumorigenicity and radio-resistance of glioblastoma. Transl Oncol 2018 Apr [PMID: 29413763]
Ha K, Ma C, Lin H et al. The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites. Nat Commun. 2017 Jun 12 [PMID: 28604711] (ICC/IF, Human) ICC/IF Human
Nadarajan S, Lambert TJ, Altendorfer E et al. Polo-like kinase-dependent phosphorylation of the synaptonemal complex protein SYP-4 regulates double-strand break formation through a negative feedback loop. Elife. Mar 27 2017 [PMID: 28346135] (ICC/IF, C. elegans) ICC/IF C. elegans
Show All 44 Publications.

Reviews for Rad51 Antibody (NB100-148) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Other.

Reviews using NB100-148:
Filter by Applications
IHC-Fr
(2)
WB
(1)
All Applications
Filter by Species
Human
(1)
Other
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-Fr Other 12/12/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23942865
SpeciesOther
 
reviewed by:
Manuela Buonanno
IHC-Fr Other 11/07/2013
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedwhole C. elegans nematode
SpeciesOther

Blocking

Blocking Details1x PBS, 0.2 % fish gelatin, 0.25 % Triton 30 m in

Primary Anitbody

Dilution Ratio1:1000 overnight 4C

Secondary Antibody

Secondary Description3 h in secondary donkey anti-rabbit Alexa-555 antibody
Secondary Concentration1:500 in blocking solution

Details

Fixation DetailsFreeze-crack tecnique
Wash Description5 washes in 1x PBS, 0.25 % Triton of 20 min each
Western Blot Rad51 NB100-148
Enlarge
reviewed by:
Western Forte
WB Human 06/20/2012
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman

Blocking

Blocking Details3% Milk in PBS

Primary Anitbody

Dilution Ratio1:2000

Secondary Antibody

Secondary Descriptionanti-mouse HRP
Secondary Manufacturer Cat#Jackson Immunoresearch
Secondary Concentration1:5000

Details

Detection NotesECL, 2 mins exposure

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for Rad51 Antibody (NB100-148) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Rad51 Antibody (NB100-148)

Discover related pathways, diseases and genes to Rad51 Antibody (NB100-148). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for Rad51 Antibody (NB100-148)

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Blogs on Rad51.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

RAD51: The cell's 'Mr. Fix-it'
RAD51 is a recombinase protein encoded by RAD51 gene in humans. Human RAD51 family members are highly similar to bacterial RecA and yeast Rad51, both biochemically and structurally. It is a 339-amino acid protein that plays an important role in homolo...  Read full blog post.

NUP153 & 53BP1: A Novel DNA Repair Pathway
Mediating DNA damage is a crucial process, and one of the most important cellular guards against cancer. In response to DNA damage, sophisticated cellular machinery is recruited to repair the breaks, and if it fails, the cell is committed to death. De...  Read full blog post.

Determining DMC1's role in Homologus Recombination
The DMC1 gene encodes a 36.7 kDa nuclear protein involved in meiotic homologous recombination. This recombinase is functionally related to the yeast RAD51 and E. coli RecA genes. In contrast to RAD51, which functions in both mitotic and meiotic recomb...  Read full blog post.

Rad51 Antibody Reveals a Canine Model for Human Breast Cancer
Our antibody catalog includes an extensive range of Rad51 antibody reagents. Encoded by the RAD51 gene, the Rad51 protein plays a vital role in DNA repair, interacting with several other proteins, including BRCA1 and BRCA2, to effect homologous recomb...  Read full blog post.

Industrial Chemicals, Tumour Suppressor Genes and the Need for More Research
Human cancer research is the largest research area in our antibody database, with new oncogenes and cell lines being added all the time.Cancer triggers come from many sources, with a worrying amount of evidence to suggest that chemicals we’re in con...  Read full blog post.

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

 
Anonymous
12/12/2014
Application: IHC-Fr
Species: Other

 
Manuela Buonanno
11/07/2013
Application: IHC-Fr
Species: Other

Western Forte
06/20/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol RAD51
Entrez
Uniprot