Rad51 Antibody (14B4)

Images

 
Western Blot: Rad51 Antibody (14B4) [NB100-148] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The signal was developed with Trident ...read more
Immunocytochemistry/ Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - staning of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody. Cells were pre-extracted with CSK buffer before fixation with 4% PFA. ...read more
Immunohistochemistry-Paraffin: Rad51 Antibody (14B4) [NB100-148] - Detects RAD51 in nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. RAD51 antibody 14B4 at a ...read more
Western Blot: Rad51 Antibody (14B4) [NB100-148] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4].
Immunoprecipitation: Rad51 Antibody (14B4) [NB100-148] - HeLa cell extract prepared with lysis 180 buffer (40 mM Tris-HCl pH8.0, 180 mM NaCl, 1 mMEDTA, 0.5% NP-40). Rabbit anti-RAD51 antibody was used for subsequent WB ...read more

Product Details

Summary
Reactivity Hu, Mu, Ce, ChSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, IP
Clone
14B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.39 mg/ml
     

Genetic Strategies

   

Rad51 Antibody (14B4) Summary

Immunogen
Full length (amino acids 1-338) Rad51 expressed in E. coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
RAD51
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:300
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100 - 1:150
  • Immunoprecipitation
  • In vitro assay
Application Notes
ChIP usage reported in scientific literature (PMID: 24023853). IHC-Fr usage reported by customer review. Use in In vitro reported in scientific literature (PMID 27815389).
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Bladder Lysate (NB820-59421)
Rad51 Lysate (NBL1-15117)
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB100-148 in the following applications:

Publications
Read Publications using
NB100-148 in the following applications:

Reactivity Notes

C.elegans reactivity reported in scientific literature (PMID: 23942865). Mouse reactivity reported in scientific literature (PMID: 25218637). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.39 mg/ml
Purity
Protein G purified

Alternate Names for Rad51 Antibody (14B4)

  • BRCC5
  • HRAD51
  • HsRad51
  • HsT16930
  • RAD51 (S. cerevisiae) homolog (E coli RecA homolog)
  • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
  • RAD51 homolog A
  • Rad51
  • RAD51ABRCA1/BRCA2-containing complex, subunit 5
  • RecA, E. coli, homolog of
  • RECADNA repair protein RAD51 homolog 1
  • RecA-like protein
  • recombination protein A

Background

Rad51, a 37kDa protein, is the human homolog of E. coli RecA protein and a member of the RAD52 epistasis group in S. cerevisiae. In recent studies it has been reported that BRCA1 interacts with Rad51 and disease-causing mutations have been found in the BRCA1 region necessary for BRCA1/Rad51 interaction, implying that this interaction is important for tumor suppression. BRCA2 and Rad51 have also been shown to interact by direct binding of the BRC repeats located within exon 11 of BRCA2. When these repeats are deleted, the interaction is lost and cells become hypersensitive to the DNA damage caused by methyl methanesulfonate (MMS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, IP

Publications for Rad51 Antibody (NB100-148)(36)

We have publications tested in 2 confirmed species: Human, C. elegans.

We have publications tested in 5 applications: ChIP, ICC/IF, IHC, In vitro, WB.


Filter By Application
ChIP
(1)
ICC/IF
(16)
IHC
(4)
In vitro
(1)
WB
(3)
All Applications
Filter By Species
Human
(9)
C. elegans
(4)
All Species
Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using NB100-148 Applications Species
Ha K, Ma C, Lin H et al. The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites. Nat Commun. 2017 Jun 12 [PMID: 28604711] (ICC/IF, Human) ICC/IF Human
Nadarajan S, Lambert TJ, Altendorfer E et al. Polo-like kinase-dependent phosphorylation of the synaptonemal complex protein SYP-4 regulates double-strand break formation through a negative feedback loop. Elife. Mar 27 2017 [PMID: 28346135] (ICC/IF, C. elegans) ICC/IF C. elegans
Pauty J, Couturier AM, Rodrigue A et al. Cancer-causing mutations in the tumor suppressor PALB2 reveal a novel cancer mechanism using a hidden nuclear export signal in the WD40 repeat motif. Nucleic Acids Res. Mar 17 2017 [PMID: 28158555]
Phillips AF, Millet AR, Tigano M et al. Single-Molecule Analysis of mtDNA Replication Uncovers the Basis of the Common Deletion Mol. Cell Jan 11 2017 [PMID: 28111015]
Li M, Yu X, Chen Q. Chemopreventive effects of ROS targeting in a murine model of BRCA1-deficient breast cancer. Cancer Res. Nov 4 2016 [PMID: 27815389] (In vitro, Human) In vitro Human
Ackermann L, Schell M, Pokrzywa W et al. E4 ligase-specific ubiquitination hubs coordinate DNA double-strand-break repair and apoptosis Nat. Struct. Mol. Biol. Sep 26 2016 [PMID: 27669035] (IHC, C. elegans) IHC C. elegans
Dok R, Abbasi Asbagh L, Van Limbergen EJ et al. Nuclear p16INK4a expression predicts enhanced radiation response in head and neck cancers. Oncotarget May 26 2016 [PMID: 27246975] (IHC, Human) IHC Human
Liu H, Yan P, Fanning E. Human DNA helicase B functions in cellular homologous recombination and stimulates rad51-mediated 5'-3' heteroduplex extension in vitro PLoS ONE. 2015 Jan 25 [PMID: 25617833] (ICC/IF, Human)

Details:
RAD51 antibody used for ICC-IF on U2OS cells which were transfected with human DNA helicase B/HDHB. The immunostaining assay involved - treatment of cells with 5 Gy IR radiation, extraction of cells with 0.2% Triton X-100 in CSK buffer, fixation of cells with 3.7 % PFA at RT for 20 minutes, primary antibody incubation at 1:300 dilution in PBS-10%FBS, detection with IF labelled secondary antibody (Figure 3)
ICC/IF Human
Cornago M, Garcia-Alberich C, Blasco-Angulo N et al. Histone deacetylase inhibitors promote glioma cell death by G2 checkpoint abrogation leading to mitotic catastrophe. Cell Death Dis. 2014 Oct 03 [PMID: 25275596] (WB, Human)

Details:
Rad51 antibody used for WB on lysates of glioma cell lines U87, U251-MG, LN229 cells as well as human GBM primary cultures C55, C65, C48 and C60 treated or not with HDAC inhibitor SAHA and valproic acid/VPA (Figure 7b and c)
WB Human
Del Nagro Christopher J, Choi Jonathan, Xiao Yang et al. Chk1 inhibition in p53-deficient cell lines drives rapid chromosome fragmentation followed by caspase-independent cell death. Cell Cycle. 2014 [PMID: 24247149] (ICC/IF, Human) ICC/IF Human
Show All 36 Publications.

Reviews for Rad51 Antibody (NB100-148) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Other.
We have 3 reviews tested in 2 application: IHC-Fr, WB.

Reviews using NB100-148:
Filter by Applications
IHC-Fr
(2)
WB
(1)
All Applications
Filter by Species
Human
(1)
Other
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-Fr Other 12/12/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23942865
SpeciesOther
 
reviewed by:
Manuela Buonanno
IHC-Fr Other 11/07/2013
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedwhole C. elegans nematode
SpeciesOther

Blocking

Blocking Details1x PBS, 0.2 % fish gelatin, 0.25 % Triton 30 m in

Primary Anitbody

Dilution Ratio1:1000 overnight 4C

Secondary Antibody

Secondary Description3 h in secondary donkey anti-rabbit Alexa-555 antibody
Secondary Concentration1:500 in blocking solution

Details

Fixation DetailsFreeze-crack tecnique
Wash Description5 washes in 1x PBS, 0.25 % Triton of 20 min each
Western Blot Rad51 NB100-148
Enlarge
reviewed by:
Western Forte
WB Human 06/20/2012
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman

Blocking

Blocking Details3% Milk in PBS

Primary Anitbody

Dilution Ratio1:2000

Secondary Antibody

Secondary Descriptionanti-mouse HRP
Secondary Manufacturer Cat#Jackson Immunoresearch
Secondary Concentration1:5000

Details

Detection NotesECL, 2 mins exposure

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Rad51 Antibody (NB100-148) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-148AF405
Alexa Fluor 488 Labeled NB100-148AF488
Alexa Fluor 647 Labeled NB100-148AF647
Alexa Fluor 700 Labeled NB100-148AF700
DyLight 350 Labeled NB100-148UV
DyLight 405 Labeled NB100-148V
DyLight 488 Labeled NB100-148G
DyLight 680 Labeled NB100-148FR
DyLight 755 Labeled NB100-148IR

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Bioinformatics Tool for Rad51 Antibody (NB100-148)

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Research Areas for Rad51 Antibody (NB100-148)

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Blogs on Rad51.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

RAD51: The cell's 'Mr. Fix-it'
RAD51 is a recombinase protein encoded by RAD51 gene in humans. Human RAD51 family members are highly similar to bacterial RecA and yeast Rad51, both biochemically and structurally. It is a 339-amino acid protein that plays an important role in homolo...  Read full blog post.

NUP153 & 53BP1: A Novel DNA Repair Pathway
Mediating DNA damage is a crucial process, and one of the most important cellular guards against cancer. In response to DNA damage, sophisticated cellular machinery is recruited to repair the breaks, and if it fails, the cell is committed to death. De...  Read full blog post.

Determining DMC1's role in Homologus Recombination
The DMC1 gene encodes a 36.7 kDa nuclear protein involved in meiotic homologous recombination. This recombinase is functionally related to the yeast RAD51 and E. coli RecA genes. In contrast to RAD51, which functions in both mitotic and meiotic recomb...  Read full blog post.

Rad51 Antibody Reveals a Canine Model for Human Breast Cancer
Our antibody catalog includes an extensive range of Rad51 antibody reagents. Encoded by the RAD51 gene, the Rad51 protein plays a vital role in DNA repair, interacting with several other proteins, including BRCA1 and BRCA2, to effect homologous recomb...  Read full blog post.

Industrial Chemicals, Tumour Suppressor Genes and the Need for More Research
Human cancer research is the largest research area in our antibody database, with new oncogenes and cell lines being added all the time.Cancer triggers come from many sources, with a worrying amount of evidence to suggest that chemicals we’re in con...  Read full blog post.

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

 
Anonymous
12/12/2014
Application: IHC-Fr
Species: Other

 
Manuela Buonanno
11/07/2013
Application: IHC-Fr
Species: Other

Western Forte
06/20/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol RAD51
Entrez
Uniprot