Rad51 Antibody (14B4)

Images

 
Western Blot: Rad51 Antibody (14B4) [NB100-148] - eIF3f facilitates hMSH4 stabilization. Western blotting analysis of the levels of HDAC3, hRad51, and VBP1 expression in 293T, 293T/eIF3f, and 293T/eIF3f-hMSH4 cells. ...read more
Immunocytochemistry/ Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - HCC1937 (human immortalized breast cancer) cells treated with PARPi for 96 h. Nuclei are stained in blue (DAPI) and RAD51 in red. ICC/IF image ...read more
Immunohistochemistry-Paraffin: Rad51 Antibody (14B4) [NB100-148] - BT483 xenograft. RAD51 antibody 14B4 dilution at 1:200. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15 min.
Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated ...read more
Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated ...read more
Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - Staining of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody. Cells were pre-extracted with CSK buffer before fixation with 4% PFA. ...read more
Immunoprecipitation: Rad51 Antibody (14B4) [NB100-148] - HeLa whole cell extract prepared with lysis 180 buffer (40 mM Tris-HCl pH8.0, 180 mM NaCl, 1 mMEDTA, 0.5% NP-40). Rabbit anti-RAD51 antibody was used for ...read more
Genetic Strategies: Knockdown Validated: Rad51 Antibody (14B4) [NB100-148] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ce, ChSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, IP, PLA, KD
Clone
14B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Rad51 Antibody (14B4) Summary

Immunogen
Full length (amino acids 1-338) Rad51 expressed in E. coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
RAD51
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:1000
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
  • Immunoprecipitation
  • In vitro assay
  • Knockdown Validated
  • Proximity Ligation Assay
  • Western Blot 1:500 - 1:3000
Application Notes
ChIP usage reported in scientific literature (PMID: 24023853). Rad51 antibody validated for IHC-Fr, ICC/IF from verified customer reviews. Use in In vitro assay reported in scientific literature (PMID: 27815389). PLA-Assay dependent.
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Bladder Whole Tissue Lysate (Adult Whole Tumor)
Rad51 Overexpression Lysate
Reviewed Applications
Read 5 Reviews rated 4.8
using
NB100-148 in the following applications:

Publications
Read Publications using
NB100-148 in the following applications:

Reactivity Notes

C. elegans reactivity reported in scientific literature (PMID: 23942865). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol (pH7)
Preservative
No Preservative
Purity
Antigen Affinity-purified

Alternate Names for Rad51 Antibody (14B4)

  • BRCC5
  • HRAD51
  • HsRad51
  • HsT16930
  • RAD51 (S. cerevisiae) homolog (E coli RecA homolog)
  • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
  • RAD51 homolog A
  • Rad51
  • RAD51ABRCA1/BRCA2-containing complex, subunit 5
  • RAD51L3
  • RecA, E. coli, homolog of
  • RECADNA repair protein RAD51 homolog 1
  • RecA-like protein
  • recombination protein A

Background

Rad51, a 37 kDa protein, is the human homolog of E. coli RecA protein and a member of the RAD52 epistasis group in S. cerevisiae. In recent studies it has been reported that BRCA1 interacts with Rad51 and disease-causing mutations have been found in the BRCA1 region necessary for BRCA1/Rad51 interaction, implying that this interaction is important for tumor suppression. BRCA2 and Rad51 have also been shown to interact by direct binding of the BRC repeats located within exon 11 of BRCA2. When these repeats are deleted, the interaction is lost and cells become hypersensitive to the DNA damage caused by methyl methanesulfonate (MMS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rad51 Antibody (NB100-148)(58)

We have publications tested in 3 confirmed species: Human, Mouse, C. elegans.

We have publications tested in 6 applications: ChIP, ICC/IF, IHC, IHC-P, In vitro, WB.


Filter By Application
ChIP
(1)
ICC/IF
(23)
IHC
(7)
IHC-P
(2)
In vitro
(1)
WB
(9)
All Applications
Filter By Species
Human
(15)
Mouse
(5)
C. elegans
(6)
All Species
Showing Publications 1 - 10 of 58. Show All 58 Publications.
Publications using NB100-148 Applications Species
Neizer-Ashun F, Dwivedi S, Dey A et al. KRCC1, a novel modulator of the DNA damage response Research Square Apr 8 2022 (WB) WB
Dong C, An L, Yu CH, Huen MSY A DYRK1B-dependent pathway suppresses rDNA transcription in response to DNA damage Nucleic acids research Jan 19 2021 [PMID: 33469661] (ICC/IF) ICC/IF
Veo BL, Danis E, Pierce A et al. Transcriptional control of DNA repair networks by CDK7 regulates sensitivity to radiation in Myc-driven Medulloblastoma bioRxiv Jan 1 2020 (IHC, IHC-P, Mouse) IHC, IHC-P Mouse
Zhou Z, Yin H, Suye S et al. The changes of DNA double-strand breaks and DNA repair during ovarian reserve formation in mice Reproductive biology Jan 10 2022 [PMID: 35026551] (IHC, Mouse) IHC Mouse
Fernando M, Duijf PHG, Proctor M et al. Dysregulated G2 phase checkpoint recovery pathway reduces DNA repair efficiency and increases chromosomal instability in a wide range of tumours Oncogenesis May 15 2021 [PMID: 33993200] (ICC/IF, WB, Human) ICC/IF, WB Human
Kaminski P, Hong S, Kono T, et al. Topoisomerase 2 beta induces DNA breaks to regulate human papillomavirus replication bioRxiv Jan 10 2021 [PMID: 33563836]
Correll-Tash S, Lilley B, Harold Salmons IV et al. Double Strand Breaks (DSBs) as Indicators of Genomic Instability in PATRR-mediated Translocations Human molecular genetics Dec 1 2020 [PMID: 33258468]
Ma, L, Ruan, J Et al. MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits. Nat Commun Nov 27 2020 [PMID: 33247137] (WB, Mouse) WB Mouse
GarcIa-Muse T Detection of DSBs in C. elegans Meiosis Methods Mol. Biol. Aug 26 2020 [PMID: 32840787]
Sriramkumar, S, Matthews, T D Et al. Platinum-Induced Ubiquitination of Phosphorylated H2AX by RING1A Is Mediated by Replication Protein A in Ovarian Cancer. Mol Cancer Res Nov 1 2020 [PMID: 32801161] (ICC/IF, Human) ICC/IF Human
Show All 58 Publications.

Reviews for Rad51 Antibody (NB100-148) (5) 4.85

Average Rating: 4.8
(Based on 5 reviews)
We have 5 reviews tested in 3 species: Human, Mouse, Other.

Reviews using NB100-148:
Filter by Applications
Immunocytochemistry
(2)
Immunohistochemistry-Frozen
(2)
Western Blot
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
Other
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Rad51 NB100-148
Enlarge
5
reviewed by:
Verified Customer
Immunocytochemistry Human 06/14/2021
View

Summary

ApplicationImmunocytochemistry
Sample Testedhuman immortalized breast cancer cells
SpeciesHuman
Lot43574

Comments

CommentsCells were fixed with 4% PFA. RAD51 Ig was diluted 1:200.
Immunocytochemistry Rad51 NB100-148
Enlarge
5
reviewed by:
Nin Wan
Immunocytochemistry Mouse 10/09/2019
View

Summary

ApplicationImmunocytochemistry
Sample Testedtestis cells
SpeciesMouse
Lot42590
  4
reviewed by:
cynthia morimoto
Immunohistochemistry-Frozen Other 12/12/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23942865
SpeciesOther
  5
reviewed by:
Verified Customer
Immunohistochemistry-Frozen Other 11/07/2013
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedwhole C. elegans nematode
SpeciesOther
Western Blot Rad51 NB100-148
Enlarge
5
reviewed by:
Verified Customer
Western Blot Human 06/20/2012
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Rad51 Antibody (NB100-148) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Rad51 Products

Bioinformatics Tool for Rad51 Antibody (NB100-148)

Discover related pathways, diseases and genes to Rad51 Antibody (NB100-148). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for Rad51 Antibody (NB100-148)

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Blogs on Rad51.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

RAD51: The cell's 'Mr. Fix-it'
RAD51 is a recombinase protein encoded by RAD51 gene in humans. Human RAD51 family members are highly similar to bacterial RecA and yeast Rad51, both biochemically and structurally. It is a 339-amino acid protein that plays an important role in homolo...  Read full blog post.

NUP153 & 53BP1: A Novel DNA Repair Pathway
Mediating DNA damage is a crucial process, and one of the most important cellular guards against cancer. In response to DNA damage, sophisticated cellular machinery is recruited to repair the breaks, and if it fails, the cell is committed to death. De...  Read full blog post.

Determining DMC1's role in Homologus Recombination
The DMC1 gene encodes a 36.7 kDa nuclear protein involved in meiotic homologous recombination. This recombinase is functionally related to the yeast RAD51 and E. coli RecA genes. In contrast to RAD51, which functions in both mitotic and meiotic recomb...  Read full blog post.

Rad51 Antibody Reveals a Canine Model for Human Breast Cancer
Our antibody catalog includes an extensive range of Rad51 antibody reagents. Encoded by the RAD51 gene, the Rad51 protein plays a vital role in DNA repair, interacting with several other proteins, including BRCA1 and BRCA2, to effect homologous recomb...  Read full blog post.

Industrial Chemicals, Tumour Suppressor Genes and the Need for More Research
Human cancer research is the largest research area in our antibody database, with new oncogenes and cell lines being added all the time.Cancer triggers come from many sources, with a worrying amount of evidence to suggest that chemicals we’re in con...  Read full blog post.

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Recent Reviews

4.8
5
4
4
1
3
0
2
0
1
0

Verified Customer
06/14/2021
Application: Immunocytochemistry
Species: Human

Nin Wan
10/09/2019
Application: Immunocytochemistry
Species: Mouse

 
cynthia morimoto
12/12/2014
Application: Immunohistochemistry-Frozen
Species: Other

Bioinformatics

Gene Symbol RAD51
Entrez
Uniprot