Rad51 Antibody (14B4)


Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at a dilution of 1:500 and developed ...read more
Immunocytochemistry/ Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - staning of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody. Cells were pre-extracted with CSK buffer before fixation with 4% PFA. ...read more
Immunohistochemistry-Paraffin: Rad51 Antibody (14B4) [NB100-148] - Detects RAD51 in nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. RAD51 antibody 14B4 at a ...read more
Western Blot: Rad51 Antibody (14B4) [NB100-148] - Analysis on Hela (lane 1) & T24 (lane 2) cell lysates.
Immunoprecipitation: Rad51 Antibody (14B4) [NB100-148] - HeLa cell extract prepared with lysis 180 buffer (40 mM Tris-HCl pH8.0, 180 mM NaCl, 1 mMEDTA, 0.5% NP-40). Rabbit anti-RAD51 antibody was used for subsequent WB ...read more

Product Details

Reactivity Hu, Mu, Ce, ChSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP
1.0 mg/ml

Rad51 Antibody (14B4) Summary

Full length (amino acids 1 - 338) Rad51 expressed in E. coli.
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.4)
No Preservative
1.0 mg/ml
Protein G purified


Application Notes
Use in Chromatin immunoprecipitation reported in scientific literature (PMID 24023853). IHC-F based on customer review. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
37 kDa
Reviewed Applications
Read 3 Reviews rated 4.7
NB100-148 in the following application:

Read Publications using
NB100-148 in the following applications:

  • 1 publication
  • 14 publications
  • IHC
    3 publications
  • WB
    3 publications

Reactivity Notes

Reactivity with C.elegans reported in scientific literature (PMID: 23942865). Mouse reactivity reported in scientific literature (PMID: 25218637). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for Rad51 Antibody (14B4)

  • BRCC5
  • HRAD51
  • HsRad51
  • HsT16930
  • RAD51 (S. cerevisiae) homolog (E coli RecA homolog)
  • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
  • RAD51 homolog A
  • RAD51ABRCA1/BRCA2-containing complex, subunit 5
  • RecA, E. coli, homolog of
  • RECADNA repair protein RAD51 homolog 1
  • RecA-like protein
  • recombination protein A


Rad51, a 37kDa protein, is the human homolog of E. coli RecA protein and a member of the RAD52 epistasis group in S. cerevisiae. In recent studies it has been reported that BRCA1 interacts with Rad51 and disease-causing mutations have been found in the BRCA1 region necessary for BRCA1/Rad51 interaction, implying that this interaction is important for tumor suppression. BRCA2 and Rad51 have also been shown to interact by direct binding of the BRC repeats located within exon 11 of BRCA2. When these repeats are deleted, the interaction is lost and cells become hypersensitive to the DNA damage caused by methyl methanesulfonate (MMS).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP
Species: Hu
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Species: Hu
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Species: Hu
Applications: ICC/IF (-), WB, IP
Species: Hu, Mu, Rt, Ch, Ha
Applications: WB, Simple Western, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu
Applications: WB, IP
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), ELISA(Sta)
Species: Mu
Applications: WB, IHC, ICC
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ELISA, ICC/IF, S-ELISA
Species: Hu, Mu, Rt, Ca
Applications: IP (-), WB, Simple Western, Flow, ICC/IF, IHC, IHC-P

Publications for Rad51 Antibody (NB100-148)(30)

We have publications tested in 2 confirmed species: Human, C. elegans.

We have publications tested in 4 applications: ChIP, ICC/IF, IHC, WB.

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C. elegans
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Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using NB100-148 Applications Species
Dok R, Abbasi Asbagh L, Van Limbergen EJ et al. Nuclear p16INK4a expression predicts enhanced radiation response in head and neck cancers. Oncotarget May 26 2016 12:00AM [PMID:27246975] (IHC, Human) IHC Human
Liu H, Yan P, Fanning E. Human DNA helicase B functions in cellular homologous recombination and stimulates rad51-mediated 5'-3' heteroduplex extension in vitro PLoS ONE. 2015 Jan 25 [PMID:25617833] (ICC/IF, Human) ICC/IF Human
Cornago M, Garcia-Alberich C, Blasco-Angulo N et al. Histone deacetylase inhibitors promote glioma cell death by G2 checkpoint abrogation leading to mitotic catastrophe. Cell Death Dis. 2014 Oct 03 [PMID:25275596] (WB, Human) WB Human
Del Nagro Christopher J, Choi Jonathan, Xiao Yang et al. Chk1 inhibition in p53-deficient cell lines drives rapid chromosome fragmentation followed by caspase-independent cell death. Cell Cycle. 2014 [PMID:24247149] (ICC/IF, Human) ICC/IF Human
Wu X, Xu Y, Feng K et al. MutS Homologue hMSH5: Recombinational DSB Repair and Non-Synonymous Polymorphic Variants. PLoS One. 2013 Sep 4 [PMID:24023853] (ChIP, Human) ChIP Human
Lans H, Lindvall JM, Thijssen K et al. DNA damage leads to progressive replicative decline but extends the life span of long-lived mutant animals. Cell Death Differ. 2013 Sep 6 [PMID:24013725] (IHC, ICC/IF, C. elegans) IHC, ICC/IF C. elegans
Buonanno M, Garty G, Grad M et al. Microbeam irradiation of C. elegans nematode in microfluidic channels. Radiat Environ Biophys. 2013 Aug 13 [PMID:23942865] (IHC, ICC/IF, C. elegans) IHC, ICC/IF C. elegans
Chu YL, Wu X, Xu Y, Her C. MutS homologue hMSH4: interaction with eIF3f and a role in NHEJ-mediated DSB repair. Mol Cancer 2013 Jun 2 [PMID:23725059] (WB, Human) WB Human
Tompkins JD, Wu X, Her C. MutS homologue hMSH5: role in cisplatin-induced DNA damage response. Mol Cancer;11:10. 2012 Mar 8. [PMID:22401567] (WB, Human) WB Human
Moldovan G-L, Dejsuphong D, Petalcorin MIR et al. Inhibition of Homologous Recombination by the PCNA-Interacting Protein PARI. Molecular Cell. 2011 Dec 6. [PMID:22153967]
Show All 30 Publications.

Reviews for Rad51 Antibody (NB100-148) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Other.
We have 3 reviews tested in 2 application: IHC-Fr, WB.

Reviews using NB100-148:
Filter by Applications
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Images Ratings Applications Species Date Details
reviewed by:
Other 12/12/2014


Sample TestedSee PMID 23942865
reviewed by:
IHC-Fr Other 11/07/2013


Sample Testedwhole C. elegans nematode


Blocking Details1x PBS, 0.2 % fish gelatin, 0.25 % Triton 30 m in

Primary Anitbody

Dilution Ratio1:1000 overnight 4C

Secondary Antibody

Secondary Description3 h in secondary donkey anti-rabbit Alexa-555 antibody
Secondary Concentration1:500 in blocking solution


Fixation DetailsFreeze-crack tecnique
Wash Description5 washes in 1x PBS, 0.25 % Triton of 20 min each
Western Blot Rad51 NB100-148
reviewed by:
WB Human 06/20/2012


ApplicationWestern Blot
Sample TestedHela whole cell lysate


Blocking Details3% Milk in PBS

Primary Anitbody

Dilution Ratio1:2000

Secondary Antibody

Secondary Descriptionanti-mouse HRP
Secondary Manufacturer Cat#Jackson Immunoresearch
Secondary Concentration1:5000


Detection NotesECL, 2 mins exposure

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Rad51 Antibody (NB100-148) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Research Areas for Rad51 Antibody (NB100-148)

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Blogs on Rad51.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

RAD51: The cell's 'Mr. Fix-it'
RAD51 is a recombinase protein encoded by RAD51 gene in humans. Human RAD51 family members are highly similar to bacterial RecA and yeast Rad51, both biochemically and structurally. It is a 339-amino acid protein that plays an important role in homolo...  Read full blog post.

NUP153 & 53BP1: A Novel DNA Repair Pathway
Mediating DNA damage is a crucial process, and one of the most important cellular guards against cancer. In response to DNA damage, sophisticated cellular machinery is recruited to repair the breaks, and if it fails, the cell is committed to death. De...  Read full blog post.

Determining DMC1's role in Homologus Recombination
The DMC1 gene encodes a 36.7 kDa nuclear protein involved in meiotic homologous recombination. This recombinase is functionally related to the yeast RAD51 and E. coli RecA genes. In contrast to RAD51, which functions in both mitotic and meiotic recomb...  Read full blog post.

Rad51 Antibody Reveals a Canine Model for Human Breast Cancer
Our antibody catalog includes an extensive range of Rad51 antibody reagents. Encoded by the RAD51 gene, the Rad51 protein plays a vital role in DNA repair, interacting with several other proteins, including BRCA1 and BRCA2, to effect homologous recomb...  Read full blog post.

Industrial Chemicals, Tumour Suppressor Genes and the Need for More Research
Human cancer research is the largest research area in our antibody database, with new oncogenes and cell lines being added all the time.Cancer triggers come from many sources, with a worrying amount of evidence to suggest that chemicals we’re in con...  Read full blog post.

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Product PDFs


Gene Symbol RAD51

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