Chk2 Antibody (73C175.1.1)

Images

 
Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 ...read more
Immunocytochemistry/ Immunofluorescence: Chk2 Antibody (73C175.1.1) [NB100-56546] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The ...read more
Immunohistochemistry-Paraffin: Chk2 Antibody (73C175.1.1) [NB100-56546] - Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody ...read more
Flow Cytometry: Chk2 Antibody (73C175.1.1) [NB100-56546] - An intracellular stain was performed on Hek293 cells with Chk2 [73C175.1.1] Antibody NB100-56546 (blue) and a matched isotype control (orange). Cells were fixed ...read more
Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
73C175.1.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Chk2 Antibody (73C175.1.1) Summary

Immunogen
A portion of amino acids 100-150 of human Chk2 protein was used as immunogen for this antibody.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CHEK2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Flow Cytometry 1-5 ug/ml
  • Immunocytochemistry/Immunofluorescence 2-5 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Control
Chk2 Overexpression Lysate
Chk2 Knockout HeLa Cell Lysate

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for Chk2 Antibody (73C175.1.1)

  • CDS1
  • CHEK2
  • CHK2 checkpoint homolog (S. pombe)
  • Chk2
  • EC 2.7.11
  • EC 2.7.11.1
  • HuCds1
  • LFS2
  • PP1425
  • Rad53
  • S.pombe) homolog

Background

Chk2 (checkpoint kinase 2) is the mammalian homolog of the Saccharomyces cerevisiae Rad53 and is required for the DNA damage and replication checkpoints (1). In response to low-dose ionizing radiation (IR), which occurs in an ataxia telangiectasia mutated (ATM)-dependent manner, Chk2 can phosphorylate the mitosis-inducing phosphatase Cdc25C on an inhibitory site, blocking entry into mitosis, and p53 on a regulatory site, causing G1 arrest (2,3). In human tissues, Chk2 is homogeneously expressed in renewing cell populations such as epidermis or intestine, heterogeneous in conditionally renewing tissues, and absent or at low level in static tissues such as muscle or brain (4). Mutations in Chk2 inactivates DNA damage checkpoint pathway involving Chk2 in lung cancer suggesting that reduced expression of Chk2 may be responsible for the development of lung cancer (5). The Chk2-/- knockout cells are resistant to DNA damage-induced apoptosis, defective for p53 stabilization and for induction of p53-dependent transcripts such as p21 in response to gamma irradiation (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Chk2 Antibody (NB100-56546) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-27403AF350
Alexa Fluor 405 NBP2-27403AF405
Alexa Fluor 488 NBP2-27403AF488
Alexa Fluor 532 NBP2-27403AF532
Alexa Fluor 594 NBP2-27403AF594
Alexa Fluor 647 NBP2-27403AF647
Alexa Fluor 700 NBP2-27403AF700
Alexa Fluor 750 NBP2-27403AF750
Biotin NBP2-27403B
DyLight 350 NBP2-27403UV
DyLight 405 NBP2-27403V
DyLight 488 NBP2-27403G
DyLight 550 NBP2-27403R
DyLight 594 NB100-56546DL594
DyLight 650 NBP2-27403C
DyLight 680 NBP2-27403FR
DyLight 755 NBP2-27403IR
FITC NBP2-27403F
HRP NBP2-27403H
Janelia Fluor 549 NBP2-27403JF549
Janelia Fluor 646 NBP2-27403JF646

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Bioinformatics

Gene Symbol CHEK2
Entrez
Uniprot