CFTR Antibody (24-1) [Unconjugated] - C-terminus

Images

 
CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Mouse Anti-Human CFTR C‑Terminus Monoclonal Antibody (Catalog # MAB25031) overnight at 4 °C. Tissue was stained with ...read more
CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clone
24-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

CFTR Antibody (24-1) [Unconjugated] - C-terminus Summary

Immunogen
Glutathione S-transferase-coupled CFTR
aa 1377-1480
Accession # P13569
Specificity
Detects in vitro synthesized CFTR and endogenous CFTR in Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CFTR
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 1-2 ug/10^6 cells
  • Western Blot 1 ug/mL
Reviewed Applications
Read 10 Reviews rated 4.2
using
MAB25031 in the following applications:

Publications
Read Publications using
MAB25031 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CFTR Antibody (24-1) [Unconjugated] - C-terminus

  • ABC35
  • ABCC7CF
  • ATP-binding cassette sub-family C member 7
  • ATP-binding cassette transporter sub-family C member 7
  • cAMP-dependent chloride channel
  • CFTR
  • CFTR/MRP
  • Channel conductance-controlling ATPase
  • cystic fibrosis transmembrane conductance regulator (ATP-binding cassettesub-family C, member 7)
  • cystic fibrosis transmembrane conductance regulator
  • cystic fibrosis transmembrane conductance regulator, ATP-binding cassette(sub-family C, member 7)
  • dJ760C5.1
  • EC 3.6.3
  • MRP7EC 3.6.3.49
  • TNR-CFTR

Background

Cystic fibrosis transmembrane conductance regulator (CFTR) is a multi-pass transmembrane protein that functions as a chloride channel. CFTR belongs to the ATP‑binding cassette (ABC) superfamily. Mutations in CFTR cause the pulmonary disease, cystic fibrosis (CF). Specifically, deletion of phenyalanine at position 508 (DeltaF508-CFTR) results in a folding defect which impairs chloride channel function. The mechanism by which channel dysfunction relates to disease symptoms is a focus of intense research. CFTR dysfunction results in disruption of ion transport and subsequent blockage of airways by secreted mucus. CFTR may also play a role in the skeletal muscle atrophy and dysfunction that characterizes CF. In addition, CFTR-mediated chloride secretion underlies fluid accumulation and cyst growth in autosomal dominant polycystic kidney disease (ADPKD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CFTR Antibody (MAB25031)(45)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Amphibian, Fish, Fish - Goldline seabream, Fish - Plotosus lineatus (Striped Eel Catfish), Ovine, Primate - Chlorocebus pygerythrus (Vervet Monkey), Xenopus.

We have publications tested in 7 applications: Co-Immunoprecipitation, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
Co-Immunoprecipitation
(1)
ICC
(3)
IHC
(3)
IHC-Fr
(1)
IHC-P
(4)
Immunoprecipitation
(10)
Western Blot
(22)
All Applications
Filter By Species
Human
(33)
Mouse
(1)
Rat
(2)
Amphibian
(1)
Fish
(3)
Fish - Goldline seabream
(1)
Fish - Plotosus lineatus (Striped Eel Catfish)
(1)
Ovine
(1)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 45. Show All 45 Publications.
Publications using MAB25031 Applications Species
SM Snow, KA Matkowskyj, M Maresh, L Clipson, TN Vo, KA Johnson, DA Deming, MA Newton, WM Grady, PJ Pickhardt, RB Halberg Validation of genetic classifiers derived from mouse and human tumors to identify molecular subtypes of colorectal cancer Human pathology, 2021;0(0):. 2021 [PMID: 34655611] (IHC, Human) IHC Human
P Kumar, DK Soni, C Sen, MB Larsen, K Mazan-Mamc, Y Piao, S De, M Gorospe, RA Frizzell, R Biswas SFPQ rescues F508del-CFTR expression and function in cystic fibrosis bronchial epithelial cells Scientific Reports, 2021;11(1):16645. 2021 [PMID: 34404863] (IHC, Human) IHC Human
M Badaoui, A Zoso, T Idris, M Bacchetta, J Simonin, S Lemeille, B Wehrle-Hal, M Chanson Vav3 Mediates Pseudomonas aeruginosa Adhesion to the Cystic Fibrosis Airway Epithelium Cell Rep, 2020;32(1):107842. 2020 [PMID: 32640241] (ICC, Human) ICC Human
M Favia, C Gallo, L Guerra, D De Venuto, A Diana, AM Polizzi, P Montemurro, MA Mariggiò, G Leonetti, A Manca, V Casavola, M Conese Treatment of Cystic Fibrosis Patients Homozygous for F508del with Lumacaftor-Ivacaftor (Orkambi�) Restores Defective CFTR Channel Function in Circulating Mononuclear Cells Int J Mol Sci, 2020;21(7):. 2020 [PMID: 32244302] (Western Blot, Human) Western Blot Human
G Ortiz-Muno, MA Yu, E Lefrançais, B Mallavia, C Valet, JJ Tian, S Ranucci, KM Wang, Z Liu, N Kwaan, D Dawson, ME Kleinhenz, FT Khasawneh, PM Haggie, AS Verkman, MR Looney Cystic fibrosis transmembrane conductance regulator dysfunction in platelets drives lung hyperinflammation J. Clin. Invest., 2020;0(0):. 2020 [PMID: 31961827] (Western Blot, Human) Western Blot Human
DC Borcherdin, ME Siefert, S Lin, J Brewington, H Sadek, JP Clancy, SM Plafker, AG Ziady Clinically-approved CFTR modulators rescue Nrf2 dysfunction in cystic fibrosis airway epithelia J. Clin. Invest., 2019;130(0):. 2019 [PMID: 31145101] (Co-Immunoprecipitation, Human) Co-Immunoprecipitation Human
S Malakpour, J Coimbra, JM Wilson Effect of dendritic organ ligation on striped eel catfish Plotosus lineatus osmoregulation PLoS ONE, 2018;13(10):e0206206. 2018 [PMID: 30352080] (IHC-P, Fish - Plotosus lineatus (Striped Eel Catfish)) IHC-P Fish - Plotosus lineatus (Striped Eel Catfish)
D De Rocco, B Pompili, S Castellani, E Morini, L Cavinato, G Cimino, MA Mariggiò, S Guarnieri, M Conese, P Del Porto, F Ascenzioni Assembly and Functional Analysis of an S/MAR Based Episome with the Cystic Fibrosis Transmembrane Conductance Regulator Gene Int J Mol Sci, 2018;19(4):. 2018 [PMID: 29673202] (Western Blot, Human) Western Blot Human
A Schögler, F Blank, M Brügger, S Beyeler, SA Tschanz, N Regamey, C Casaulta, T Geiser, MP Alves Characterization of pediatric cystic fibrosis airway epithelial cell cultures at the air-liquid interface obtained by non-invasive nasal cytology brush sampling Respir. Res., 2017;18(1):215. 2017 [PMID: 29282053] (ICC, Human) ICC Human
CL Shrestha, KD Assani, H Rinehardt, F Albastroiu, S Zhang, R Shell, AO Amer, LS Schlesinge, BT Kopp Cysteamine-mediated clearance of antibiotic-resistant pathogens in human cystic fibrosis macrophages PLoS ONE, 2017;12(10):e0186169. 2017 [PMID: 28982193] (Western Blot, Human) Western Blot Human
Show All 45 Publications.

Reviews for CFTR Antibody (MAB25031) (10) 4.210

Average Rating: 4.2
(Based on 10 reviews)
We have 10 reviews tested in 3 species: Human, Mouse, Other.

Reviews using MAB25031:
Filter by Applications
WB
(3)
IF
(4)
IP
(1)
IHC-Fr
(1)
Flow
(1)
All Applications
Filter by Species
Human
(5)
Mouse
(4)
Other
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
cynthia morimoto
WB Mouse 02/19/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23060444
SpeciesMouse
  5
reviewed by:
cynthia morimoto
IF Other 02/19/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 20107177
SpeciesOther
  4
reviewed by:
cynthia morimoto
WB Human 02/19/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21811577
SpeciesHuman
  4
reviewed by:
cynthia morimoto
WB Human 02/19/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22904170
SpeciesHuman
  4
reviewed by:
cynthia morimoto
IP Human 02/19/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedSee PMID 21383017
SpeciesHuman
  4
reviewed by:
cynthia morimoto
IHC-Fr Mouse 02/19/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23045527
SpeciesMouse
  4
reviewed by:
cynthia morimoto
IF Mouse 02/19/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24204804
SpeciesMouse
  4
reviewed by:
cynthia morimoto
IF Mouse 02/19/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22989883
SpeciesMouse
  4
reviewed by:
cynthia morimoto
IF Human 02/19/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 20351101
SpeciesHuman
  4
reviewed by:
cynthia morimoto
Flow Human 02/19/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 21561312
SpeciesHuman

Product General Protocols

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Video Protocols

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FAQs for CFTR Antibody (MAB25031) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

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Bioinformatics Tool for CFTR Antibody (MAB25031)

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Blogs on CFTR.

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Recent Reviews

4.2
5
2
4
8
3
0
2
0
1
0

 
cynthia morimoto
02/19/2015
Application: WB
Species: Mouse

 
cynthia morimoto
02/19/2015
Application: IF
Species: Other

 
cynthia morimoto
02/19/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol CFTR
Uniprot