CFTR Antibody (24-1) [Unconjugated] - C-terminus

CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Chronic exposure to cigarette smoke (CS) decreases airway surface liquid (ASL) height. Primary human airway epithelial cells from 4 donors (n = 8) were exposed to 30 puffs of whole cigarette smoke (2 cigarettes) every day for 5 days (120 hrs). (A) ASL height was measured one hour after each exposure to CS. ASL height was undisturbed over the course of the reading. *p < 0.05. (B) CFTR present at the plasma membrane was detected by immunoblotting after biotinylation of cell surface proteins (see Methods). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of S. aureus supernatant on CFTR localisation and expression. (A, B) Immunolocalisation of CFTR (green staining) and Dapi nuclei staining (blue) in lateral view of successive z level images. In control cells, we noticed an apical staining of CFTR (arrow heads in A). In 2%S. aureus supernatant-treated cells (B), the CFTR staining was more diffuse in the cytoplasm. (C) Western blotting analysis of airway glandular cell membrane proteins showed the presence of CFTR in control cells and in fewer amount in cells incubated with 2%S. aureus supernatant. (D) Quantitative measurement showed a significant (*, p < 0.05) decrease in CFTR expression in cell membranes when cells were incubated with 2% S. aureus supernatant. Data represent the mean ± SEM of 5 different experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20089165), licensed under a CC-BY license. Not internally tested by R&D Systems.

Cigarette smoke extract (CSE) decreases the expression of CFTR but not Na+/K+-ATPase in human bronchial epithelial cells. 16HBE14o- cells were treated with 10% CSE for up to 48 hours (A) or increasing concentrations of CSE prepared from commercial grade cigarettes (Camel) for 48 hours (B). CFTR and Na+/K+-ATPase were detected by immunoblotting. The same amount of protein was loaded in each lane as indicated by detection of beta -actin. The blots are representative of at least three independent experiments. (C) Detection of CFTR mRNA transcript levels using quantitative RT-PCR analysis after treatment of 16HBE14o- cells with 10% CSE for 24 hours. Results are expressed as fold change and are representative of three independent experiments. *p < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemical localization of transporters in gills of tilapia acclimated to different salinities.Representative micrographs of immunohistolocalization of Nka (green) with either Nkcc/Ncc (a–c; red) or Cftr (d–f; red) in the gills of tilapia acclimated to FW (a,d), SW (b,e) or HSW (c,f). Co-localization of red and green fluorochromes results in yellow-orange staining. Higher magnification (10×) of boxed areas in (a–f) correspond to panels (a′–f′). Sections are counter stained with the nuclear stain DAPI and overlaid with the DIC image for tissue orientation. Arrows indicate apical staining and arrowheads tubular system (basolateral) staining. Scale bar 100 µm (a–f), 10 µm (a′–c′). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0087591), licensed under a CC-BY license. Not internally tested by R&D Systems.

COMMD1 regulates CFTR ubiquitination.(A) Representative gels for the same CFTR IP experiment with MAB25031 from HeLa cells stably expressing wt-CFTR and separated on 8% SDS-PAGE transferred to PVDF membrane. Half of the membrane was probed with anti-CFTR mAb and the other half with anti-ubiquitin mAb. Lysates were loaded onto an 11% SDS-PAGE and sequential probing of the membrane was performed (COMMD1, alpha -tubulin and lastly ubiquitin). Filled and empty arrowheads indicate the fully- (170 kDa) and core-glycosylated (140 kDa) CFTR, respectively. (B) Quantification of ubiquitinated CFTR. Ratio of ubiquitinated CFTR to total CFTR in each condition is shown, endogenous COMMD1 expression is referred as 100%. The means ± S.D. were obtained from five independent experiments.* P<0.05 was determined by t-test. (C) Stability of the mature wt-CFTR was determined upon inhibition of protein biosynthesis with cycloheximide (CHX). Cells were incubated in the presence of cycloheximide for the indicated time intervals. (D) Quantification of mature CFTR was normalized to alpha -tubulin level. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0018334), licensed under a CC-BY license. Not internally tested by R&D Systems.

Co-localisation by immunofluorescence of CFTR and actin. (A) The pattern of CFTR (green staining) and actin (red staining) stainings was essentially apical in control cells as well as in cells treated with Sal/FP (B). (C) The incubation of cells with S. aureus supernatant induced alteration of the localisation of CFTR that appeared to be cytoplasmic, in parallel with a disorganization of the actin network. (D) Treatment of S. aureus supernatant pre-incubated cells with Sal/FP restored CFTR and actin apical stainings. (E) Quantification of the co-localisation of CFTR and actin showed that 2% S. aureus supernatant decreased the co-localisation index compared to the index in control cells, but the difference was not significant; the treatment with Sal/FP alone or after S. aureus supernatant incubation significantly enhanced the co-localisation of the 2 proteins compared with control or with S. aureus supernatant-treated cells (*, p < 0.05). Data represent the mean ± SEM of 3 different experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20089165), licensed under a CC-BY license. Not internally tested by R&D Systems.

COMMD1 and CFTR interact in mammalian cells.(A) Sequences of ICL3 in other species from fish to primates. Asterisks indicate the position of two class II mutations: S945L and D979A. Identity of amino acids between the different proteins are boxed in black, conserved residues are boxed in dark gray and semi-conserved substitutions in light gray. (B) Representative gels for the same co-immunoprecipitation experiments in HT-29 cells expressing endogenous CFTR and COMMD1. Lysates from HT-29 cells were immunoprecipitated (IP) with either 0.8 µg of anti-COMMD1 mAb (Abnova), 0.8 µg of anti-CFTR mAb (MAB25031, R&D Systems) or with 0.8 µg anti-mouse IgG as a control. Each immunoprecipitation sample was then split in half and loaded onto an 8% SDS-PAGE for CFTR detection and 11% SDS-PAGE for COMMD1 detection. Both gels were transferred to PVDF membrane and subjected to immunoblotting (IB). The 8% SDS-PAGE membrane was probed with anti-CFTR mAb (MM13-4) and the 11% SDS-PAGE membrane with a rabbit anti-COMMD1 pAb (Proteintech Group). Both membranes were probed with anti-alpha -tubulin as control (11% gel is shown). Filled and empty arrowheads indicate the fully- (170 kDa) and core-glycosylated (140 kDa) CFTR, respectively. * indicates mouse IgG light chain from the antibody used for immunoprecipitation. (C) COMMD1 constructions in pcDNA3.1/Topo plasmid. Two COMMD1 constructs were generated by adding a Myc-tag at the N-terminus of COMMD1: Myc-COMMD1 and a construct with a deletion of the COMM domain named Myc-COMMD1 delta COMM. (D) Representative gels for the same co-immunoprecipitation experiment between COMMD1 and wt- in heterologous system. HeLa cells stably expressing wt- (spTCF-wt) or empty CFTR vector (spTracer) as control were transfected with Myc-COMMD1. spTCF-wt were transfected with Myc-COMMD1 delta COMM. Lysates from all these experiments were subjected to SDS-PAGE, as in (B) after CFTR IP. The 8% SDS-PAGE membrane was probed with anti-CFTR mAb and the 11% SDS-PAGE membrane with anti-c-Myc mAb. Both membranes were probed with anti-alpha -tubulin as control (11% gel is shown). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0018334), licensed under a CC-BY license. Not internally tested by R&D Systems.

Manganese and cadmium decrease the expression of CFTR in bronchial epithelial cells. 16HBE14o- cells were incubated with cadmium chloride (CdCl2) or manganese chloride (MnCl2) at the doses indicated for 24 hours. CFTR protein was detected by immunobloting using a monoclonal antibody as described in Materials and Methods. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of S. aureus supernatant on CFTR localisation and expression. (A, B) Immunolocalisation of CFTR (green staining) and Dapi nuclei staining (blue) in lateral view of successive z level images. In control cells, we noticed an apical staining of CFTR (arrow heads in A). In 2%S. aureus supernatant-treated cells (B), the CFTR staining was more diffuse in the cytoplasm. (C) Western blotting analysis of airway glandular cell membrane proteins showed the presence of CFTR in control cells and in fewer amount in cells incubated with 2%S. aureus supernatant. (D) Quantitative measurement showed a significant (*, p < 0.05) decrease in CFTR expression in cell membranes when cells were incubated with 2% S. aureus supernatant. Data represent the mean ± SEM of 5 different experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20089165), licensed under a CC-BY license. Not internally tested by R&D Systems.

Cigarette smoke extract (CSE) decreases the expression of CFTR but not Na+/K+-ATPase in human bronchial epithelial cells. 16HBE14o- cells were treated with 10% CSE for up to 48 hours (A) or increasing concentrations of CSE prepared from commercial grade cigarettes (Camel) for 48 hours (B). CFTR and Na+/K+-ATPase were detected by immunoblotting. The same amount of protein was loaded in each lane as indicated by detection of beta -actin. The blots are representative of at least three independent experiments. (C) Detection of CFTR mRNA transcript levels using quantitative RT-PCR analysis after treatment of 16HBE14o- cells with 10% CSE for 24 hours. Results are expressed as fold change and are representative of three independent experiments. *p < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemical localization of transporters in gills of tilapia acclimated to different salinities.Representative micrographs of immunohistolocalization of Nka (green) with either Nkcc/Ncc (a–c; red) or Cftr (d–f; red) in the gills of tilapia acclimated to FW (a,d), SW (b,e) or HSW (c,f). Co-localization of red and green fluorochromes results in yellow-orange staining. Higher magnification (10×) of boxed areas in (a–f) correspond to panels (a′–f′). Sections are counter stained with the nuclear stain DAPI and overlaid with the DIC image for tissue orientation. Arrows indicate apical staining and arrowheads tubular system (basolateral) staining. Scale bar 100 µm (a–f), 10 µm (a′–c′). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0087591), licensed under a CC-BY license. Not internally tested by R&D Systems.

Metals present in CSE regulate CFTR expression. 16HBE14o- cells were incubated with 10% CSE before and after incubation with Chelex-100 beads, in absence or presence of 10 μM cadmium chloride. CFTR protein was detected by immunoblotting 48 hours after treatment. Blots are representative of at least three independent experiments. *p < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemical localization of transporters in gills of tilapia acclimated to different salinities.Representative micrographs of immunohistolocalization of Nka (green) with either Nkcc/Ncc (a–c; red) or Cftr (d–f; red) in the gills of tilapia acclimated to FW (a,d), SW (b,e) or HSW (c,f). Co-localization of red and green fluorochromes results in yellow-orange staining. Higher magnification (10×) of boxed areas in (a–f) correspond to panels (a′–f′). Sections are counter stained with the nuclear stain DAPI and overlaid with the DIC image for tissue orientation. Arrows indicate apical staining and arrowheads tubular system (basolateral) staining. Scale bar 100 µm (a–f), 10 µm (a′–c′). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0087591), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemical localization of transporters in anterior and posterior intestine of tilapia acclimated to different salinities.Representative micrographs of immunolocalization of Nka (green) with Nkcc/Ncc (red) (a–d, f–h) from FW (a, f), SW (b, g) and HSW (c, h) acclimated tilapia. (d) A representative higher magnification micrograph of Nkcc/Ncc staining of the brush border of enterocytes with basolateral Nka staining from the anterior intestine of SW-acclimated fish. (e) Apical Cftr (red) double labeling with Nka (green) in the anterior intestine of a FW-acclimated fish. Panels (a–e) are sections of anterior intestine (AI) while panels (f–h) are sections of posterior intestine (PI). Sections are counter stained with the nuclear stain DAPI and overlaid with the DIC image for tissue orientation. Scale bar 100 µm (a–c, f–h); 25 µm (d,e). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0087591), licensed under a CC-BY license. Not internally tested by R&D Systems.

COMMD1 regulates CFTR ubiquitination.(A) Representative gels for the same CFTR IP experiment with MAB25031 from HeLa cells stably expressing wt-CFTR and separated on 8% SDS-PAGE transferred to PVDF membrane. Half of the membrane was probed with anti-CFTR mAb and the other half with anti-ubiquitin mAb. Lysates were loaded onto an 11% SDS-PAGE and sequential probing of the membrane was performed (COMMD1, alpha -tubulin and lastly ubiquitin). Filled and empty arrowheads indicate the fully- (170 kDa) and core-glycosylated (140 kDa) CFTR, respectively. (B) Quantification of ubiquitinated CFTR. Ratio of ubiquitinated CFTR to total CFTR in each condition is shown, endogenous COMMD1 expression is referred as 100%. The means ± S.D. were obtained from five independent experiments.* P<0.05 was determined by t-test. (C) Stability of the mature wt-CFTR was determined upon inhibition of protein biosynthesis with cycloheximide (CHX). Cells were incubated in the presence of cycloheximide for the indicated time intervals. (D) Quantification of mature CFTR was normalized to alpha -tubulin level. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/21483833), licensed under a CC-BY license. Not internally tested by R&D Systems.

COMMD1 regulates CFTR cell surface expression.(A) HeLa cells stably expressing wt-CFTR were transiently transfected with an empty COMMD1 vector (mock, pcDNA3.1/Topo) or Myc-COMMD1, and were biotinylated with Sulfo-NHS-LC-biotin. Lysates from all these experiments were subjected to SDS-PAGE directly (input) or pulled-down with streptavidin-agarose (biotin). Representative gels for the same samples were separated by 8% SDS-PAGE for CFTR, Na/K-ATPase detection and 11% SDS-PAGE for COMMD1, alpha -tubulin detection. (B) HeLa cells stably expressing wt-CFTR were transiently transfected with a siCONTROL Non-Targeting siRNA (mock) or COMMD1 siRNA and further processed as in (A). Filled and empty arrowheads indicate the fully- (170 kDa) and core-glycosylated (140 kDa) CFTR, respectively. (C) Quantification of CFTR cell surface expression. The biotinylated CFTR level is normalized to the biotinylated Na/K-ATPase level. Endogenous COMMD1 expression is referred as 100%, with mock being pcDNA3.1/Topo for overexpression experiments (A), whereas mock was siCONTROL for silencing experiments (B). The means ± S.D. were obtained from three independent experiments.* P<0.05 was determined by t-test. (D) Immunofluorescence microscopy of COMMD1 and CFTR in HeLa cells stably expressing wt-CFTR. Cells were transfected with Myc-COMMD1 or COMMD1 siRNA for overexpression and silencing studies, respectively, and not transfected for endogenous expression studies. Two types of light exposure microscopy (short and normal) are shown to visualize all expression conditions. Scale bars: 10 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/21483833), licensed under a CC-BY license. Not internally tested by R&D Systems.

COMMD1 regulates CFTR cell surface expression.(A) HeLa cells stably expressing wt-CFTR were transiently transfected with an empty COMMD1 vector (mock, pcDNA3.1/Topo) or Myc-COMMD1, and were biotinylated with Sulfo-NHS-LC-biotin. Lysates from all these experiments were subjected to SDS-PAGE directly (input) or pulled-down with streptavidin-agarose (biotin). Representative gels for the same samples were separated by 8% SDS-PAGE for CFTR, Na/K-ATPase detection and 11% SDS-PAGE for COMMD1, alpha -tubulin detection. (B) HeLa cells stably expressing wt-CFTR were transiently transfected with a siCONTROL Non-Targeting siRNA (mock) or COMMD1 siRNA and further processed as in (A). Filled and empty arrowheads indicate the fully- (170 kDa) and core-glycosylated (140 kDa) CFTR, respectively. (C) Quantification of CFTR cell surface expression. The biotinylated CFTR level is normalized to the biotinylated Na/K-ATPase level. Endogenous COMMD1 expression is referred as 100%, with mock being pcDNA3.1/Topo for overexpression experiments (A), whereas mock was siCONTROL for silencing experiments (B). The means ± S.D. were obtained from three independent experiments.* P<0.05 was determined by t-test. (D) Immunofluorescence microscopy of COMMD1 and CFTR in HeLa cells stably expressing wt-CFTR. Cells were transfected with Myc-COMMD1 or COMMD1 siRNA for overexpression and silencing studies, respectively, and not transfected for endogenous expression studies. Two types of light exposure microscopy (short and normal) are shown to visualize all expression conditions. Scale bars: 10 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/21483833), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clone: 24-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CFTR Antibody (24-1) [Unconjugated] - C-terminus Summary

• Immunogen: Glutathione S-transferase-coupled CFTR
  aa 1377-1480
  Accession # P13569
• Specificity: Detects in vitro synthesized CFTR and endogenous CFTR in Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CFTR
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 1-2 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CFTR Antibody (24-1) [Unconjugated] - C-terminus

• ABC35
• ABCC7CF
• ATP-binding cassette sub-family C member 7
• ATP-binding cassette transporter sub-family C member 7
• cAMP-dependent chloride channel
• CFTR
• CFTR/MRP
• Channel conductance-controlling ATPase
• cystic fibrosis transmembrane conductance regulator (ATP-binding cassettesub-family C, member 7)
• cystic fibrosis transmembrane conductance regulator
• cystic fibrosis transmembrane conductance regulator, ATP-binding cassette(sub-family C, member 7)
• dJ760C5.1
• EC 3.6.3
• MRP7EC 3.6.3.49
• TNR-CFTR

Background

Cystic fibrosis transmembrane conductance regulator (CFTR) is a multi-pass transmembrane protein that functions as a chloride channel. CFTR belongs to the ATP‑binding cassette (ABC) superfamily. Mutations in CFTR cause the pulmonary disease, cystic fibrosis (CF). Specifically, deletion of phenyalanine at position 508 (DeltaF508-CFTR) results in a folding defect which impairs chloride channel function. The mechanism by which channel dysfunction relates to disease symptoms is a focus of intense research. CFTR dysfunction results in disruption of ion transport and subsequent blockage of airways by secreted mucus. CFTR may also play a role in the skeletal muscle atrophy and dysfunction that characterizes CF. In addition, CFTR-mediated chloride secretion underlies fluid accumulation and cyst growth in autosomal dominant polycystic kidney disease (ADPKD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CFTR Antibody (MAB25031)(76)

Publications using MAB25031

• Caohuy, H;Eidelman, O;Chen, T;Mungunsukh, O;Yang, Q;Walton, NI;Pollard, BS;Khanal, S;Hentschel, S;Florez, C;Herbert, AS;Pollard, HB; Inflammation in the COVID-19 airway is due to inhibition of CFTR signaling by the SARS-CoV-2 spike protein Scientific reports 2024-07-23 [PMID: 39043712] (Western Blot, Primate - Cercopithecus aethiops (African Green Monkey))
• Li, C;Liu, Z;Anderson, J;Liu, Z;Tang, L;Li, Y;Peng, N;Chen, J;Liu, X;Fu, L;Townes, TM;Rowe, SM;Bedwell, DM;Guimbellot, J;Zhao, R; Prime editing-mediated correction of the CFTR W1282X mutation in iPSCs and derived airway epithelial cells PloS one 2023-11-29 [PMID: 38019847] (ICC, Human)
• Qin-Tong Hou, Xiao-Liang Xu, Jian-Wen Wang, Xiao-Liang Dai, Li Wang, Cong-Ling Jiang et al. Loss-of-function CFTR p.G970D missense mutation might cause congenital bilateral absence of the vas deferens and be associated with impaired spermatogenesis Asian Journal of Andrology 2023-01-01 [PMID: 35665694]
• Nathaniel J. Henning, Lydia Boike, Jessica N. Spradlin, Carl C. Ward, Gang Liu, Erika Zhang et al. Deubiquitinase-targeting chimeras for targeted protein stabilization Nature Chemical Biology 2022-04-01 [PMID: 35210618]
• SM Snow, KA Matkowskyj, M Maresh, L Clipson, TN Vo, KA Johnson, DA Deming, MA Newton, WM Grady, PJ Pickhardt, RB Halberg Validation of genetic classifiers derived from mouse and human tumors to identify molecular subtypes of colorectal cancer Human pathology, 2021-10-14;0(0):. 2021-10-14 [PMID: 34655611] (IHC, Human)
• P Kumar, DK Soni, C Sen, MB Larsen, K Mazan-Mamc, Y Piao, S De, M Gorospe, RA Frizzell, R Biswas SFPQ rescues F508del-CFTR expression and function in cystic fibrosis bronchial epithelial cells Scientific Reports, 2021-08-17;11(1):16645. 2021-08-17 [PMID: 34404863] (IHC, Human)
• Chun Chih Chen, William S. Marshall, George N. Robertson, Regina R. F. Cozzi, Scott P. Kelly Mummichog gill and operculum exhibit functionally consistent claudin-10 paralog profiles and Claudin-10c hypersaline response Biology Open 2021-07-15 [PMID: 34308991]
• M Badaoui, A Zoso, T Idris, M Bacchetta, J Simonin, S Lemeille, B Wehrle-Hal, M Chanson Vav3 Mediates Pseudomonas aeruginosa Adhesion to the Cystic Fibrosis Airway Epithelium Cell Rep, 2020-07-07;32(1):107842. 2020-07-07 [PMID: 32640241] (ICC, Human)
• Ilan M. Ruhr, Chris M. Wood, Kevin L. Schauer, Yadong Wang, Edward M. Mager, Bruce Stanton et al. Is aquaporin‐3 involved in water‐permeability changes in the killifish during hypoxia and normoxic recovery, in freshwater or seawater? Journal of Experimental Zoology Part A: Ecological and Integrative Physiology 2020-08-01 [PMID: 32548921]
• Rehman T, Thornell IM, Pezzulo AA et al. TNFalpha and IL-17 Alkalinize Airway Surface Liquid through CFTR and Pendrin Am. J. Physiol., Cell Physiol. 5/20/2020 [PMID: 32432926]
• M Favia, C Gallo, L Guerra, D De Venuto, A Diana, AM Polizzi, P Montemurro, MA Mariggiò, G Leonetti, A Manca, V Casavola, M Conese Treatment of Cystic Fibrosis Patients Homozygous for F508del with Lumacaftor-Ivacaftor (Orkambi�) Restores Defective CFTR Channel Function in Circulating Mononuclear Cells Int J Mol Sci, 2020-03-31;21(7):. 2020-03-31 [PMID: 32244302] (Western Blot, Human)
• G Ortiz-Muno, MA Yu, E Lefrançais, B Mallavia, C Valet, JJ Tian, S Ranucci, KM Wang, Z Liu, N Kwaan, D Dawson, ME Kleinhenz, FT Khasawneh, PM Haggie, AS Verkman, MR Looney Cystic fibrosis transmembrane conductance regulator dysfunction in platelets drives lung hyperinflammation J. Clin. Invest., 2020-04-01;0(0):. 2020-04-01 [PMID: 31961827] (Western Blot, Human)
• DC Borcherdin, ME Siefert, S Lin, J Brewington, H Sadek, JP Clancy, SM Plafker, AG Ziady Clinically-approved CFTR modulators rescue Nrf2 dysfunction in cystic fibrosis airway epithelia J. Clin. Invest., 2019-05-30;130(0):. 2019-05-30 [PMID: 31145101] (Co-Immunoprecipitation, Human)
• Marozkina N, Bosch J, Cotton C et al. Cyclic compression increases F508 Del CFTR expression in ciliated human airway epithelium American Journal of Physiology-Lung Cellular and Molecular Physiology 2019-08-01 [PMID: 31116581]
• Zeljka Trepotec, Johannes Geiger, Christian Plank, Manish K. Aneja, Carsten Rudolph Segmented poly(A) tails significantly reduce recombination of plasmid DNA without affecting mRNA translation efficiency or half-life RNA 2019-04-01 [PMID: 30647100] (Western Blot, Human)
• Emily Xia, Yiqian Zhang, Huibi Cao, Jun Li, Rongqi Duan, Jim Hu TALEN-Mediated Gene Targeting for Cystic Fibrosis-Gene Therapy Genes (Basel) 2019-01-11 [PMID: 30641980]
• S Malakpour, J Coimbra, JM Wilson Effect of dendritic organ ligation on striped eel catfish Plotosus lineatus osmoregulation PLoS ONE, 2018-10-23;13(10):e0206206. 2018-10-23 [PMID: 30352080] (IHC-P, Fish - Plotosus lineatus (Striped Eel Catfish))
• D De Rocco, B Pompili, S Castellani, E Morini, L Cavinato, G Cimino, MA Mariggiò, S Guarnieri, M Conese, P Del Porto, F Ascenzioni Assembly and Functional Analysis of an S/MAR Based Episome with the Cystic Fibrosis Transmembrane Conductance Regulator Gene Int J Mol Sci, 2018-04-17;19(4):. 2018-04-17 [PMID: 29673202] (ICC, Western Blot, Human)
• Annalucia Carbone, Roberto Zefferino, Elisa Beccia, Valeria Casavola, Stefano Castellani, Sante Di Gioia et al. Gap Junctions Are Involved in the Rescue of CFTR-Dependent Chloride Efflux by Amniotic Mesenchymal Stem Cells in Coculture with Cystic Fibrosis CFBE41o- Cells Stem Cells International 2018-01-11 [PMID: 29531530]
• Ilan M. Ruhr, Kevin L. Schauer, Yoshio Takei, Martin Grosell Renoguanylin stimulates apical CFTR translocation and decreases HCO3− secretion through PKA activity in the Gulf toadfish (Opsanus beta) Journal of Experimental Biology 2018-01-01 [PMID: 29361605]
• A Schögler, F Blank, M Brügger, S Beyeler, SA Tschanz, N Regamey, C Casaulta, T Geiser, MP Alves Characterization of pediatric cystic fibrosis airway epithelial cell cultures at the air-liquid interface obtained by non-invasive nasal cytology brush sampling Respir. Res., 2017-12-28;18(1):215. 2017-12-28 [PMID: 29282053] (ICC, Human)
• Marty Kwok Shing Wong, Takehiro Tsukada, Nobuhiro Ogawa, Supriya Pipil, Haruka Ozaki, Yutaka Suzuki et al. A sodium binding system alleviates acute salt stress during seawater acclimation in eels Zoological Letters 2017-12-12 [PMID: 29255617]
• CL Shrestha, KD Assani, H Rinehardt, F Albastroiu, S Zhang, R Shell, AO Amer, LS Schlesinge, BT Kopp Cysteamine-mediated clearance of antibiotic-resistant pathogens in human cystic fibrosis macrophages PLoS ONE, 2017-10-05;12(10):e0186169. 2017-10-05 [PMID: 28982193] (Western Blot, Human)
• K Arora, Y Huang, K Mun, S Yarlagadda, N Sundaram, MM Kessler, G Hannig, CB Kurtz, I Silos-Sant, M Helmrath, JJ Palermo, JP Clancy, KA Steinbrech, AP Naren Guanylate cyclase 2C agonism corrects CFTR mutants JCI Insight, 2017-10-05;2(19):. 10/5/2017 [PMID: 28978796]
• Roberto Plebani, Romina Tripaldi, Paola Lanuti, Antonio Recchiuti, Sara Patruno, Sara Di Silvestre et al. Establishment and long-term culture of human cystic fibrosis endothelial cells Laboratory Investigation 2017-11-01 [PMID: 28759010]
• MF Figueira, RC Castiglion, CM de Lemos B, FM Ornellas, G da Silva F, MM Morales, RN da Fonseca, J de Souza-M Diabetic rats present higher urinary loss of proteins and lower renal expression of megalin, cubilin, ClC-5, and CFTR Physiol Rep, 2017-07-01;5(13):. 2017-07-01 [PMID: 28676554] (Western Blot, Rat)
• XL Chen, B Zhang, YR Chng, JLY Ong, SF Chew, WP Wong, SH Lam, T Nakada, YK Ip Ammonia exposure affects the mRNA and protein expression levels of certain Rhesus glycoproteins in the gills of climbing perch J. Exp. Biol., 2017-06-02;0(0):. 2017-06-02 [PMID: 28576822] (IHC-P, Fish)
• W. S. Marshall, R. R. F. Cozzi, M. Spieker WNK1 and p38-MAPK distribution in ionocytes and accessory cells of euryhaline teleost fish implies ionoregulatory function Biology Open 2017-07-15 [PMID: 28522431]
• Z Bozoky, S Ahmadi, T Milman, TH Kim, K Du, M Di Paola, S Pasyk, R Pekhletski, JP Keller, CE Bear, JD Forman-Kay Synergy of cAMP and calcium signaling pathways in CFTR regulation Proc. Natl. Acad. Sci. U.S.A, 2017-02-27;0(0):. 2017-02-27 [PMID: 28242698] (Human)
• Israel Hanukoglu, Vijay R. Boggula, Hananya Vaknine, Sachin Sharma, Thomas Kleyman, Aaron Hanukoglu Expression of epithelial sodium channel (ENaC) and CFTR in the human epidermis and epidermal appendages Histochemistry and Cell Biology 2017-06-01 [PMID: 28130590]
• Deep interactome profiling of membrane proteins by co-interacting protein identification technology Nat Protoc, 2016-11-17;11(12):2515-2528. 2016-11-17 [PMID: 27854364] (Immunoprecipitation, Human)
• Agathe Tarze New use for an old drug: COX-independent anti-inflammatory effects of sulindac in models of cystic fibrosis Br. J. Pharmacol., 2016-04-21;173(11):1728-41. 2016-04-21 [PMID: 26894321] (Western Blot, Human)
• Anna C. Abbattiscianni, Maria Favia, Maria T. Mancini, Rosa A. Cardone, Lorenzo Guerra, Stefania Monterisi et al. Correctors of mutant CFTR enhance subcortical cAMP-PKA signaling through modulating ezrin phosphorylation and cytoskeleton organization Journal of Cell Science 2016-03-15 [PMID: 26823603]
• Jessica Bellec, Marc Bacchetta, Davide Losa, Ignacio Anegon, Marc Chanson, Tuan Huy Nguyen <i>CFTR</i> Inactivation by Lentiviral Vector-mediated RNA Interference and CRISPR-Cas9 Genome Editing in Human Airway Epithelial Cells Current Gene Therapy 2015-08-25 [PMID: 26264708]
• Barbara Dhooghe, Charlotte Bouckaert, Arnaud Capron, Pierre Wallemacq, Teresinha Leal, Sabrina Noel Resveratrol increases F508del-CFTR dependent salivary secretion in cystic fibrosis mice Biology Open 2015-07-15 [PMID: 26092868]
• Fumiya Furukawa, Soichi Watanabe, Andre P. Seale, Jason P. Breves, Darren T. Lerner, E Gordon Gordon Grau et al. In vivo and in vitro effects of high-K(+) stress on branchial expression of ROMKa in seawater-acclimated Mozambique tilapia Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 2015-09-01 [PMID: 26021981]
• Tabeling C, Yu H, Wang L, Ranke H, Goldenberg N, Zabini D, Noe E, Krauszman A, Gutbier B, Yin J, Schaefer M, Arenz C, Hocke A, Suttorp N, Proia R, Witzenrath M, Kuebler W CFTR and sphingolipids mediate hypoxic pulmonary vasoconstriction. Proc Natl Acad Sci U S A, 2015-03-17;112(13):E1614-23. 2015-03-17 [PMID: 25829545] (Immunoprecipitation, Western Blot, Mouse)
• Ferru-Clement R, Fresquet F, Norez C, Metaye T, Becq F, Kitzis A, Thoreau V Involvement of the Cdc42 pathway in CFTR post-translational turnover and in its plasma membrane stability in airway epithelial cells. PLoS ONE, 2015-03-13;10(3):e0118943. 2015-03-13 [PMID: 25768293] (Western Blot, Human)
• Caohuy H, Yang Q, Eudy Y, Ha T, Xu A, Glover M, Frizzell R, Jozwik C, Pollard H Activation of 3-phosphoinositide-dependent kinase 1 (PDK1) and serum- and glucocorticoid-induced protein kinase 1 (SGK1) by short-chain sphingolipid C4-ceramide rescues the trafficking defect of DeltaF508-cystic fibrosis transmembrane conductance regulator (DeltaF508-CFTR). J Biol Chem, 2014-11-10;289(52):35953-68. 2014-11-10 [PMID: 25384981] (ICC, Immunoprecipitation, Western Blot, Human)
• Runzhen Zhao, Xinrong Liang, Meimi Zhao, Shan-Lu Liu, Yao Huang, Steven Idell et al. Correlation of Apical Fluid-Regulating Channel Proteins with Lung Function in Human COPD Lungs PLoS ONE 2014-10-17 [PMID: 25329998]
• Pascale Marcorelles, Gaëlle Friocourt, Arnaud Uguen, Françoise Ledé, Claude Férec, Annie Laquerrière Cystic fibrosis transmembrane conductance regulator protein (CFTR) expression in the developing human brain: comparative immunohistochemical study between patients with normal and mutated CFTR Journal of Histochemistry & Cytochemistry 2014-11-01 [PMID: 25062999]
• Hassan F, Xu X, Nuovo G, Killilea D, Tyrrell J, Da Tan C, Tarran R, Diaz P, Jee J, Knoell D, Boyaka P, Cormet-Boyaka E Accumulation of metals in GOLD4 COPD lungs is associated with decreased CFTR levels. Respir Res, 2014-06-23;15(0):69. 2014-06-23 [PMID: 24957904] (Western Blot, Human)
• Carbone A, Castellani S, Favia M, Diana A, Paracchini V, Di Gioia S, Seia M, Casavola V, Colombo C, Conese M Correction of defective CFTR/ENaC function and tightness of cystic fibrosis airway epithelium by amniotic mesenchymal stromal (stem) cells. J Cell Mol Med, 2014-06-03;18(8):1631-43. 2014-06-03 [PMID: 24894806] (Flow Cytometry, Western Blot, Human)
• Maria Favia, Maria T. Mancini, Valentino Bezzerri, Lorenzo Guerra, Onofrio Laselva, Anna C. Abbattiscianni et al. Trimethylangelicin promotes the functional rescue of mutant F508del CFTR protein in cystic fibrosis airway cells American Journal of Physiology-Lung Cellular and Molecular Physiology 2014-07-01 [PMID: 24816489]
• Losa D, Kohler T, Bellec J, Dudez T, Crespin S, Bacchetta M, Boulanger P, Hong S, Morel S, Nguyen T, van Delden C, Chanson M Pseudomonas aeruginosa-induced apoptosis in airway epithelial cells is mediated by gap junctional communication in a JNK-dependent manner. J Immunol, 2014-04-14;192(10):4804-12. 2014-04-14 [PMID: 24733844] (Western Blot, Human)
• Li Z, Lui E, Wilson J, Ip Y, Lin Q, Lam T, Lam S Expression of key ion transporters in the gill and esophageal-gastrointestinal tract of euryhaline Mozambique tilapia Oreochromis mossambicus acclimated to fresh water, seawater and hypersaline water. PLoS ONE, 2014-01-31;9(1):e87591. 2014-01-31 [PMID: 24498146] (IHC-P, Fish)
• Cebotaru L, Rapino D, Cebotaru V, Guggino W Correcting the cystic fibrosis disease mutant, A455E CFTR. PLoS ONE, 2014-01-08;9(1):e85183. 2014-01-08 [PMID: 24416359] (Western Blot, Primate - Chlorocebus pygerythrus (Vervet Monkey))
• Barbara Dhooghe, Sabrina Noël, Caroline Bouzin, Gaëtane Behets-Wydemans, Teresinha Leal Correction of Chloride Transport and Mislocalization of CFTR Protein by Vardenafil in the Gastrointestinal Tract of Cystic Fibrosis Mice PLoS ONE 2013-10-24 [PMID: 24204804]
• El Khouri E, Le Pavec G, Toledano M, Delaunay-Moisan A RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR). J Biol Chem, 2013-09-09;288(43):31177-91. 2013-09-09 [PMID: 24019521] (Immunoprecipitation, Human)
• Viart V, Varilh J, Lopez E, Rene C, Claustres M, Taulan-Cadars M Phosphorylated C/EBPbeta influences a complex network involving YY1 and USF2 in lung epithelial cells. PLoS ONE, 2013-04-01;8(4):e60211. 2013-04-01 [PMID: 23560079] (Immunoprecipitation, Human)
• Oglesby I, Chotirmall S, McElvaney N, Greene C Regulation of cystic fibrosis transmembrane conductance regulator by microRNA-145, -223, and -494 is altered in DeltaF508 cystic fibrosis airway epithelium. J Immunol, 2013-02-22;190(7):3354-62. 2013-02-22 [PMID: 23436935] (Western Blot, Human)
• Duan , Yuanyuan, Sun , Ying, Zhang , Fan, Zhang , Wei Kevi, Wang , Dong, Wang , Yan, Cao , Xu, Hu , Wenbao, Xie , Changyan, Cuppoletti , John, Magin , Thomas M, Wang , Haixia, Wu , Zhenguo, Li , Ning, Huang , Pingbo Keratin K18 increases cystic fibrosis transmembrane conductance regulator (CFTR) surface expression by binding to its C-terminal hydrophobic patch. J Biol Chem, 2012-10-08;287(48):40547-59. 2012-10-08 [PMID: 23045527] (Immunoprecipitation, Human)
• Murphy , Sean V, Lim , Rebecca, Heraud , Philip, Cholewa , Marian, Le Gros , Mark, de Jonge , Martin D, Howard , Daryl L, Paterson , David, McDonald , Courtney, Atala , Anthony, Jenkin , Graham, Wallace , Euan M Human amnion epithelial cells induced to express functional cystic fibrosis transmembrane conductance regulator. PLoS ONE, 2012-09-28;7(9):e46533. 2012-09-28 [PMID: 23029546] (Flow Cytometry, ICC, Western Blot, Human)
• King , J Darwin, Lee , Jeffrey, Riemen , Claudia, Neumann , Dietbert, Xiong , Sheng, Foskett , J Kevin, Mehta , Anil, Muimo , Richmond, Hallows , Kenneth Role of binding and nucleoside diphosphate kinase A in the regulation of the cystic fibrosis transmembrane conductance regulator by AMP-activated protein kinase. J Biol Chem, 2012-08-06;287(40):33389-400. 2012-08-06 [PMID: 22869372] (Immunoprecipitation, Western Blot, Human)
• Teng L, Kerbiriou M, Taiya M, Le Hir S, Mignen O, Benz N, Trouve P, Ferec C Proteomic Identification of Calumenin as a G551D - CFTR Associated Protein. PLoS ONE, 2012-06-29;7(6):e40173. 2012-06-29 [PMID: 22768251] (Western Blot, Human)
• Valentina Paracchini, Annalucia Carbone, Federico Colombo, Stefano Castellani, Silvia Mazzucchelli, Sante Di Di Gioia et al. Amniotic Mesenchymal Stem Cells: A New Source for Hepatocyte-Like Cells and Induction of CFTR Expression by Coculture with Cystic Fibrosis Airway Epithelial Cells Journal of Biomedicine and Biotechnology 2012-01-22 [PMID: 22315512]
• Sorio C, Buffelli M, Angiari C, Ettorre M, Johansson J, Vezzalini M, Viviani L, Ricciardi M, Verze G, Assael BM, Melotti P Defective CFTR expression and function are detectable in blood monocytes: development of a new blood test for cystic fibrosis. PLoS ONE, 2011-07-21;6(7):e22212. 2011-07-21 [PMID: 21811577] (Western Blot, Human)
• Donatella Piro, Claudia Piccoli, Lorenzo Guerra, Francesca Sassone, Annamaria D'Aprile, Maria Favia et al. Hematopoietic Stem/Progenitor Cells Express Functional Mitochondrial Energy-Dependent Cystic Fibrosis Transmembrane Conductance Regulator Stem Cells and Development 2012-03-01 [PMID: 21561312]
• Drevillon L, Tanguy G, Hinzpeter A et al. COMMD1-Mediated Ubiquitination Regulates CFTR Trafficking. PLoS One. 3/31/2011 [PMID: 21483833]
• Jean-Marie Zahm, Franck Delavoie, Férial Toumi, Béatrice Nawrocki-Raby, Claire Kileztky, Jean Michel et al. Long acting beta2-agonist and corticosteroid restore airway glandular cell function altered by bacterial supernatant Respiratory Research 2010-12-01 [PMID: 20089165]
• Antonius Bronckers, Lida Kalogeraki, Huub J.N. Jorna, Martina Wilke, Theodore J. Bervoets, Donacian M. Lyaruu et al. The cystic fibrosis transmembrane conductance regulator (CFTR) is expressed in maturation stage ameloblasts, odontoblasts and bone cells Bone 2010-04-01 [PMID: 20004757]
• D. J. McKeon, K. A. Cadwallader, S. Idris, A. S. Cowburn, M. C. Pasteur, H. Barker et al. Cystic fibrosis neutrophils have normal intrinsic reactive oxygen species generation European Respiratory Journal 2010-06-01 [PMID: 19840964]
• Fouassier L, Rosenberg P, Mergey M, Saubamea B, Claperon A, Kinnman N, Chignard N, Jacobsson-Ekman G, Strandvik B, Rey C, Barbu V, Hultcrantz R, Housset C Ezrin-radixin-moesin-binding phosphoprotein (EBP50), an estrogen-inducible scaffold protein, contributes to biliary epithelial cell proliferation. Am. J. Pathol., 2009-03-01;174(3):869-80. 2009-03-01 [PMID: 19234136] (IHC, Human)
• Cebotaru L, Vij N, Ciobanu I, Wright J, Flotte T, Guggino WB Cystic fibrosis transmembrane regulator missing the first four transmembrane segments increases wild type and DeltaF508 processing. J. Biol. Chem., 2008-05-28;283(32):21926-33. 2008-05-28 [PMID: 18508776] (Western Blot, Human)
• Shead EF, Haworth CS, Condliffe AM, McKeon DJ, Scott MA, Compston JE Cystic fibrosis transmembrane conductance regulator (CFTR) is expressed in human bone. Thorax, 2007-07-01;62(7):650-1. 2007-07-01 [PMID: 17600296] (ICC, IHC, Western Blot, Human)
• Robert R, Savineau JP, Norez C, Becq F, Guibert C Expression and function of cystic fibrosis transmembrane conductance regulator in rat intrapulmonary arteries. Eur. Respir. J., 2007-06-27;30(5):857-64. 2007-06-27 [PMID: 17596272] (ICC, Immunoprecipitation, Rat)
• Madsen SS, Jensen LN, Tipsmark CK, Kiilerich P, Borski RJ Differential regulation of cystic fibrosis transmembrane conductance regulator and Na+,K+ -ATPase in gills of striped bass, Morone saxatilis: effect of salinity and hormones. J. Endocrinol., 2007-01-01;192(1):249-60. 2007-01-01 [PMID: 17210762] (IHC-P, Western Blot, Fish)
• Tang JY, Au DW The ichthyotoxic alga Chattonella marina induces Na+, K+ -ATPase, and CFTR proteins expression in fish gill chloride cells in vivo. Biochem. Biophys. Res. Commun., 2006-12-04;353(1):98-103. 2006-12-04 [PMID: 17161378] (ICC, Western Blot, Fish - Goldline seabream)
• Painter RG, Valentine VG, Lanson NA, Leidal K, Zhang Q, Lombard G, Thompson C, Viswanathan A, Nauseef WM, Wang G CFTR Expression in human neutrophils and the phagolysosomal chlorination defect in cystic fibrosis. Biochemistry, 2006-08-29;45(34):10260-9. 2006-08-29 [PMID: 16922501] (ICC, Western Blot, Human)
• Nanua S, Sajjan U, Keshavjee S, Hershenson MB Absence of typical unfolded protein response in primary cultured cystic fibrosis airway epithelial cells. Biochem. Biophys. Res. Commun., 2006-03-03;343(1):135-43. 2006-03-03 [PMID: 16530168] (ICC, Western Blot, Human)
• Davidson H, McLachlan G, Wilson A, Boyd AC, Doherty A, MacGregor G, Davies L, Painter HA, Coles R, Hyde SC, Gill DR, Amaral MD, Collie DD, Porteous DJ, Penque D Human-specific cystic fibrosis transmembrane conductance regulator antibodies detect in vivo gene transfer to ovine airways. Am. J. Respir. Cell Mol. Biol., 2006-02-23;35(1):72-83. 2006-02-23 [PMID: 16498081] (IHC-Fr, Ovine)
• Clain J, Lehmann-Che J, Dugueperoux I, Arous N, Girodon E, Legendre M, Goossens M, Edelman A, de Braekeleer M, Teulon J, Fanen P Misprocessing of the CFTR protein leads to mild cystic fibrosis phenotype. Hum. Mutat., 2005-04-01;25(4):360-71. 2005-04-01 [PMID: 15776432] (ICC, Immunoprecipitation, Human)
• Bilan F, Thoreau V, Nacfer M, Derand R, Norez C, Cantereau A, Garcia M, Becq F, Kitzis A Syntaxin 8 impairs trafficking of cystic fibrosis transmembrane conductance regulator (CFTR) and inhibits its channel activity. J. Cell. Sci., 2004-03-23;117(0):1923-35. 2004-03-23 [PMID: 15039462] (Immunoprecipitation, Human)
• Willumsen NJ, Amstrup J, Mobjerg N, Jespersen A, Kristensen P, Larsen EH Mitochondria-rich cells as experimental model in studies of epithelial chloride channels. Biochim. Biophys. Acta, 2002-11-13;1566(1):28-43. 2002-11-13 [PMID: 12421535] (IHC-P, Xenopus)
• Pan J, Bear C, Farragher S, Cutz E, Yeger H Cystic fibrosis transmembrane conductance regulator modulates neurosecretory function in pulmonary neuroendocrine cell-related tumor cell line models. Am. J. Respir. Cell Mol. Biol., 2002-11-01;27(5):553-60. 2002-11-01 [PMID: 12397014] (ICC, Human)
• Bagorda A, Guerra L, Di Sole F, Hemle-Kolb C, Cardone RA, Fanelli T, Reshkin SJ, Gisler SM, Murer H, Casavola V Reciprocal protein kinase A regulatory interactions between cystic fibrosis transmembrane conductance regulator and Na+/H+ exchanger isoform 3 in a renal polarized epithelial cell model. J. Biol. Chem., 2002-04-05;277(24):21480-8. 2002-04-05 [PMID: 11937500] (Western Blot, Amphibian)

Reviews for CFTR Antibody (MAB25031) (10)

Average Rating: 4.2

(Based on 10 reviews)

We have 10 reviews tested in 3 species: Human, Mouse, Other.

reviewed by: Verified Customer WB Mouse 02/19/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23060444
• Species: Mouse

reviewed by: Verified Customer IF Other 02/19/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 20107177
• Species: Other

reviewed by: Verified Customer WB Human 02/19/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 21811577
• Species: Human

reviewed by: Verified Customer WB Human 02/19/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 22904170
• Species: Human

reviewed by: Verified Customer IP Human 02/19/2015

Summary

• Application: Immunoprecipitation
• Sample Tested: See PMID 21383017
• Species: Human

reviewed by: Verified Customer IHC-Fr Mouse 02/19/2015

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: See PMID 23045527
• Species: Mouse

reviewed by: Verified Customer IF Mouse 02/19/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 24204804
• Species: Mouse

reviewed by: Verified Customer IF Mouse 02/19/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 22989883
• Species: Mouse

reviewed by: Verified Customer IF Human 02/19/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 20351101
• Species: Human

reviewed by: Verified Customer Flow Human 02/19/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 21561312
• Species: Human

Bioinformatics

• Gene Symbol: CFTR
• Uniprot:
  • P13569()