CFTR Antibody (24-1) [Unconjugated] - C-terminus

CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Mouse Anti-Human CFTR C‑Terminus Monoclonal Antibody (Catalog # MAB25031) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clone: 24-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CFTR Antibody (24-1) [Unconjugated] - C-terminus Summary

• Immunogen: Glutathione S-transferase-coupled CFTR
  aa 1377-1480
  Accession # P13569
• Specificity: Detects in vitro synthesized CFTR and endogenous CFTR in Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CFTR
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 1-2 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CFTR Antibody (24-1) [Unconjugated] - C-terminus

• ABC35
• ABCC7CF
• ATP-binding cassette sub-family C member 7
• ATP-binding cassette transporter sub-family C member 7
• cAMP-dependent chloride channel
• CFTR
• CFTR/MRP
• Channel conductance-controlling ATPase
• cystic fibrosis transmembrane conductance regulator (ATP-binding cassettesub-family C, member 7)
• cystic fibrosis transmembrane conductance regulator
• cystic fibrosis transmembrane conductance regulator, ATP-binding cassette(sub-family C, member 7)
• dJ760C5.1
• EC 3.6.3
• MRP7EC 3.6.3.49
• TNR-CFTR

Background

Cystic fibrosis transmembrane conductance regulator (CFTR) is a multi-pass transmembrane protein that functions as a chloride channel. CFTR belongs to the ATP‑binding cassette (ABC) superfamily. Mutations in CFTR cause the pulmonary disease, cystic fibrosis (CF). Specifically, deletion of phenyalanine at position 508 (DeltaF508-CFTR) results in a folding defect which impairs chloride channel function. The mechanism by which channel dysfunction relates to disease symptoms is a focus of intense research. CFTR dysfunction results in disruption of ion transport and subsequent blockage of airways by secreted mucus. CFTR may also play a role in the skeletal muscle atrophy and dysfunction that characterizes CF. In addition, CFTR-mediated chloride secretion underlies fluid accumulation and cyst growth in autosomal dominant polycystic kidney disease (ADPKD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CFTR Antibody (MAB25031)(45)

Publications using MAB25031

• SM Snow, KA Matkowskyj, M Maresh, L Clipson, TN Vo, KA Johnson, DA Deming, MA Newton, WM Grady, PJ Pickhardt, RB Halberg Validation of genetic classifiers derived from mouse and human tumors to identify molecular subtypes of colorectal cancer Human pathology, 2021-10-14;0(0):. 2021-10-14 [PMID: 34655611] (IHC, Human)
• P Kumar, DK Soni, C Sen, MB Larsen, K Mazan-Mamc, Y Piao, S De, M Gorospe, RA Frizzell, R Biswas SFPQ rescues F508del-CFTR expression and function in cystic fibrosis bronchial epithelial cells Scientific Reports, 2021-08-17;11(1):16645. 2021-08-17 [PMID: 34404863] (IHC, Human)
• M Badaoui, A Zoso, T Idris, M Bacchetta, J Simonin, S Lemeille, B Wehrle-Hal, M Chanson Vav3 Mediates Pseudomonas aeruginosa Adhesion to the Cystic Fibrosis Airway Epithelium Cell Rep, 2020-07-07;32(1):107842. 2020-07-07 [PMID: 32640241] (ICC, Human)
• M Favia, C Gallo, L Guerra, D De Venuto, A Diana, AM Polizzi, P Montemurro, MA Mariggiò, G Leonetti, A Manca, V Casavola, M Conese Treatment of Cystic Fibrosis Patients Homozygous for F508del with Lumacaftor-Ivacaftor (Orkambi�) Restores Defective CFTR Channel Function in Circulating Mononuclear Cells Int J Mol Sci, 2020-03-31;21(7):. 2020-03-31 [PMID: 32244302] (Western Blot, Human)
• G Ortiz-Muno, MA Yu, E Lefrançais, B Mallavia, C Valet, JJ Tian, S Ranucci, KM Wang, Z Liu, N Kwaan, D Dawson, ME Kleinhenz, FT Khasawneh, PM Haggie, AS Verkman, MR Looney Cystic fibrosis transmembrane conductance regulator dysfunction in platelets drives lung hyperinflammation J. Clin. Invest., 2020-04-01;0(0):. 2020-04-01 [PMID: 31961827] (Western Blot, Human)
• DC Borcherdin, ME Siefert, S Lin, J Brewington, H Sadek, JP Clancy, SM Plafker, AG Ziady Clinically-approved CFTR modulators rescue Nrf2 dysfunction in cystic fibrosis airway epithelia J. Clin. Invest., 2019-05-30;130(0):. 2019-05-30 [PMID: 31145101] (Co-Immunoprecipitation, Human)
• S Malakpour, J Coimbra, JM Wilson Effect of dendritic organ ligation on striped eel catfish Plotosus lineatus osmoregulation PLoS ONE, 2018-10-23;13(10):e0206206. 2018-10-23 [PMID: 30352080] (IHC-P, Fish - Plotosus lineatus (Striped Eel Catfish))
• D De Rocco, B Pompili, S Castellani, E Morini, L Cavinato, G Cimino, MA Mariggiò, S Guarnieri, M Conese, P Del Porto, F Ascenzioni Assembly and Functional Analysis of an S/MAR Based Episome with the Cystic Fibrosis Transmembrane Conductance Regulator Gene Int J Mol Sci, 2018-04-17;19(4):. 2018-04-17 [PMID: 29673202] (ICC, Western Blot, Human)
• A Schögler, F Blank, M Brügger, S Beyeler, SA Tschanz, N Regamey, C Casaulta, T Geiser, MP Alves Characterization of pediatric cystic fibrosis airway epithelial cell cultures at the air-liquid interface obtained by non-invasive nasal cytology brush sampling Respir. Res., 2017-12-28;18(1):215. 2017-12-28 [PMID: 29282053] (ICC, Human)
• CL Shrestha, KD Assani, H Rinehardt, F Albastroiu, S Zhang, R Shell, AO Amer, LS Schlesinge, BT Kopp Cysteamine-mediated clearance of antibiotic-resistant pathogens in human cystic fibrosis macrophages PLoS ONE, 2017-10-05;12(10):e0186169. 2017-10-05 [PMID: 28982193] (Western Blot, Human)
• MF Figueira, RC Castiglion, CM de Lemos B, FM Ornellas, G da Silva F, MM Morales, RN da Fonseca, J de Souza-M Diabetic rats present higher urinary loss of proteins and lower renal expression of megalin, cubilin, ClC-5, and CFTR Physiol Rep, 2017-07-01;5(13):. 2017-07-01 [PMID: 28676554] (Western Blot, Rat)
• XL Chen, B Zhang, YR Chng, JLY Ong, SF Chew, WP Wong, SH Lam, T Nakada, YK Ip Ammonia exposure affects the mRNA and protein expression levels of certain Rhesus glycoproteins in the gills of climbing perch J. Exp. Biol., 2017-06-02;0(0):. 2017-06-02 [PMID: 28576822] (IHC-P, Fish)
• Z Bozoky, S Ahmadi, T Milman, TH Kim, K Du, M Di Paola, S Pasyk, R Pekhletski, JP Keller, CE Bear, JD Forman-Kay Synergy of cAMP and calcium signaling pathways in CFTR regulation Proc. Natl. Acad. Sci. U.S.A, 2017-02-27;0(0):. 2017-02-27 [PMID: 28242698] (Human)
• Deep interactome profiling of membrane proteins by co-interacting protein identification technology Nat Protoc, 2016-11-17;11(12):2515-2528. 2016-11-17 [PMID: 27854364] (Immunoprecipitation, Human)
• Agathe Tarze New use for an old drug: COX-independent anti-inflammatory effects of sulindac in models of cystic fibrosis Br. J. Pharmacol., 2016-04-21;173(11):1728-41. 2016-04-21 [PMID: 26894321] (Western Blot, Human)
• Tabeling C, Yu H, Wang L, Ranke H, Goldenberg N, Zabini D, Noe E, Krauszman A, Gutbier B, Yin J, Schaefer M, Arenz C, Hocke A, Suttorp N, Proia R, Witzenrath M, Kuebler W CFTR and sphingolipids mediate hypoxic pulmonary vasoconstriction. Proc Natl Acad Sci U S A, 2015-03-17;112(13):E1614-23. 2015-03-17 [PMID: 25829545] (Immunoprecipitation, Western Blot, Mouse)
• Ferru-Clement R, Fresquet F, Norez C, Metaye T, Becq F, Kitzis A, Thoreau V Involvement of the Cdc42 pathway in CFTR post-translational turnover and in its plasma membrane stability in airway epithelial cells. PLoS ONE, 2015-03-13;10(3):e0118943. 2015-03-13 [PMID: 25768293] (Western Blot, Human)
• Caohuy H, Yang Q, Eudy Y, Ha T, Xu A, Glover M, Frizzell R, Jozwik C, Pollard H Activation of 3-phosphoinositide-dependent kinase 1 (PDK1) and serum- and glucocorticoid-induced protein kinase 1 (SGK1) by short-chain sphingolipid C4-ceramide rescues the trafficking defect of DeltaF508-cystic fibrosis transmembrane conductance regulator (DeltaF508-CFTR). J Biol Chem, 2014-11-10;289(52):35953-68. 2014-11-10 [PMID: 25384981] (ICC, Immunoprecipitation, Western Blot, Human)
• Hassan F, Xu X, Nuovo G, Killilea D, Tyrrell J, Da Tan C, Tarran R, Diaz P, Jee J, Knoell D, Boyaka P, Cormet-Boyaka E Accumulation of metals in GOLD4 COPD lungs is associated with decreased CFTR levels. Respir Res, 2014-06-23;15(0):69. 2014-06-23 [PMID: 24957904] (Western Blot, Human)
• Carbone A, Castellani S, Favia M, Diana A, Paracchini V, Di Gioia S, Seia M, Casavola V, Colombo C, Conese M Correction of defective CFTR/ENaC function and tightness of cystic fibrosis airway epithelium by amniotic mesenchymal stromal (stem) cells. J Cell Mol Med, 2014-06-03;18(8):1631-43. 2014-06-03 [PMID: 24894806] (Flow Cytometry, Western Blot, Human)
• Losa D, Kohler T, Bellec J, Dudez T, Crespin S, Bacchetta M, Boulanger P, Hong S, Morel S, Nguyen T, van Delden C, Chanson M Pseudomonas aeruginosa-induced apoptosis in airway epithelial cells is mediated by gap junctional communication in a JNK-dependent manner. J Immunol, 2014-04-14;192(10):4804-12. 2014-04-14 [PMID: 24733844] (Western Blot, Human)
• Li Z, Lui E, Wilson J, Ip Y, Lin Q, Lam T, Lam S Expression of key ion transporters in the gill and esophageal-gastrointestinal tract of euryhaline Mozambique tilapia Oreochromis mossambicus acclimated to fresh water, seawater and hypersaline water. PLoS ONE, 2014-01-31;9(1):e87591. 2014-01-31 [PMID: 24498146] (IHC-P, Fish)
• Cebotaru L, Rapino D, Cebotaru V, Guggino W Correcting the cystic fibrosis disease mutant, A455E CFTR. PLoS ONE, 2014-01-08;9(1):e85183. 2014-01-08 [PMID: 24416359] (Western Blot, Primate - Chlorocebus pygerythrus (Vervet Monkey))
• El Khouri E, Le Pavec G, Toledano M, Delaunay-Moisan A RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR). J Biol Chem, 2013-09-09;288(43):31177-91. 2013-09-09 [PMID: 24019521] (Immunoprecipitation, Human)
• Viart V, Varilh J, Lopez E, Rene C, Claustres M, Taulan-Cadars M Phosphorylated C/EBPbeta influences a complex network involving YY1 and USF2 in lung epithelial cells. PLoS ONE, 2013-04-01;8(4):e60211. 2013-04-01 [PMID: 23560079] (Immunoprecipitation, Human)
• Oglesby I, Chotirmall S, McElvaney N, Greene C Regulation of cystic fibrosis transmembrane conductance regulator by microRNA-145, -223, and -494 is altered in DeltaF508 cystic fibrosis airway epithelium. J Immunol, 2013-02-22;190(7):3354-62. 2013-02-22 [PMID: 23436935] (Western Blot, Human)
• Duan , Yuanyuan, Sun , Ying, Zhang , Fan, Zhang , Wei Kevi, Wang , Dong, Wang , Yan, Cao , Xu, Hu , Wenbao, Xie , Changyan, Cuppoletti , John, Magin , Thomas M, Wang , Haixia, Wu , Zhenguo, Li , Ning, Huang , Pingbo Keratin K18 increases cystic fibrosis transmembrane conductance regulator (CFTR) surface expression by binding to its C-terminal hydrophobic patch. J Biol Chem, 2012-10-08;287(48):40547-59. 2012-10-08 [PMID: 23045527] (Immunoprecipitation, Human)
• Murphy , Sean V, Lim , Rebecca, Heraud , Philip, Cholewa , Marian, Le Gros , Mark, de Jonge , Martin D, Howard , Daryl L, Paterson , David, McDonald , Courtney, Atala , Anthony, Jenkin , Graham, Wallace , Euan M Human amnion epithelial cells induced to express functional cystic fibrosis transmembrane conductance regulator. PLoS ONE, 2012-09-28;7(9):e46533. 2012-09-28 [PMID: 23029546] (Flow Cytometry, ICC, Western Blot, Human)
• King , J Darwin, Lee , Jeffrey, Riemen , Claudia, Neumann , Dietbert, Xiong , Sheng, Foskett , J Kevin, Mehta , Anil, Muimo , Richmond, Hallows , Kenneth Role of binding and nucleoside diphosphate kinase A in the regulation of the cystic fibrosis transmembrane conductance regulator by AMP-activated protein kinase. J Biol Chem, 2012-08-06;287(40):33389-400. 2012-08-06 [PMID: 22869372] (Immunoprecipitation, Western Blot, Human)
• Teng L, Kerbiriou M, Taiya M, Le Hir S, Mignen O, Benz N, Trouve P, Ferec C Proteomic Identification of Calumenin as a G551D - CFTR Associated Protein. PLoS ONE, 2012-06-29;7(6):e40173. 2012-06-29 [PMID: 22768251] (Western Blot, Human)
• Sorio C, Buffelli M, Angiari C, Ettorre M, Johansson J, Vezzalini M, Viviani L, Ricciardi M, Verze G, Assael BM, Melotti P Defective CFTR expression and function are detectable in blood monocytes: development of a new blood test for cystic fibrosis. PLoS ONE, 2011-07-21;6(7):e22212. 2011-07-21 [PMID: 21811577] (Western Blot, Human)
• Fouassier L, Rosenberg P, Mergey M, Saubamea B, Claperon A, Kinnman N, Chignard N, Jacobsson-Ekman G, Strandvik B, Rey C, Barbu V, Hultcrantz R, Housset C Ezrin-radixin-moesin-binding phosphoprotein (EBP50), an estrogen-inducible scaffold protein, contributes to biliary epithelial cell proliferation. Am. J. Pathol., 2009-03-01;174(3):869-80. 2009-03-01 [PMID: 19234136] (IHC, Human)
• Cebotaru L, Vij N, Ciobanu I, Wright J, Flotte T, Guggino WB Cystic fibrosis transmembrane regulator missing the first four transmembrane segments increases wild type and DeltaF508 processing. J. Biol. Chem., 2008-05-28;283(32):21926-33. 2008-05-28 [PMID: 18508776] (Western Blot, Human)
• Shead EF, Haworth CS, Condliffe AM, McKeon DJ, Scott MA, Compston JE Cystic fibrosis transmembrane conductance regulator (CFTR) is expressed in human bone. Thorax, 2007-07-01;62(7):650-1. 2007-07-01 [PMID: 17600296] (ICC, IHC, Western Blot, Human)
• Robert R, Savineau JP, Norez C, Becq F, Guibert C Expression and function of cystic fibrosis transmembrane conductance regulator in rat intrapulmonary arteries. Eur. Respir. J., 2007-06-27;30(5):857-64. 2007-06-27 [PMID: 17596272] (ICC, Immunoprecipitation, Rat)
• Madsen SS, Jensen LN, Tipsmark CK, Kiilerich P, Borski RJ Differential regulation of cystic fibrosis transmembrane conductance regulator and Na+,K+ -ATPase in gills of striped bass, Morone saxatilis: effect of salinity and hormones. J. Endocrinol., 2007-01-01;192(1):249-60. 2007-01-01 [PMID: 17210762] (IHC-P, Western Blot, Fish)
• Tang JY, Au DW The ichthyotoxic alga Chattonella marina induces Na+, K+ -ATPase, and CFTR proteins expression in fish gill chloride cells in vivo. Biochem. Biophys. Res. Commun., 2006-12-04;353(1):98-103. 2006-12-04 [PMID: 17161378] (ICC, Western Blot, Fish - Goldline seabream)
• Painter RG, Valentine VG, Lanson NA, Leidal K, Zhang Q, Lombard G, Thompson C, Viswanathan A, Nauseef WM, Wang G CFTR Expression in human neutrophils and the phagolysosomal chlorination defect in cystic fibrosis. Biochemistry, 2006-08-29;45(34):10260-9. 2006-08-29 [PMID: 16922501] (ICC, Western Blot, Human)
• Nanua S, Sajjan U, Keshavjee S, Hershenson MB Absence of typical unfolded protein response in primary cultured cystic fibrosis airway epithelial cells. Biochem. Biophys. Res. Commun., 2006-03-03;343(1):135-43. 2006-03-03 [PMID: 16530168] (ICC, Western Blot, Human)
• Davidson H, McLachlan G, Wilson A, Boyd AC, Doherty A, MacGregor G, Davies L, Painter HA, Coles R, Hyde SC, Gill DR, Amaral MD, Collie DD, Porteous DJ, Penque D Human-specific cystic fibrosis transmembrane conductance regulator antibodies detect in vivo gene transfer to ovine airways. Am. J. Respir. Cell Mol. Biol., 2006-02-23;35(1):72-83. 2006-02-23 [PMID: 16498081] (IHC-Fr, Ovine)
• Clain J, Lehmann-Che J, Dugueperoux I, Arous N, Girodon E, Legendre M, Goossens M, Edelman A, de Braekeleer M, Teulon J, Fanen P Misprocessing of the CFTR protein leads to mild cystic fibrosis phenotype. Hum. Mutat., 2005-04-01;25(4):360-71. 2005-04-01 [PMID: 15776432] (ICC, Immunoprecipitation, Human)
• Bilan F, Thoreau V, Nacfer M, Derand R, Norez C, Cantereau A, Garcia M, Becq F, Kitzis A Syntaxin 8 impairs trafficking of cystic fibrosis transmembrane conductance regulator (CFTR) and inhibits its channel activity. J. Cell. Sci., 2004-03-23;117(0):1923-35. 2004-03-23 [PMID: 15039462] (Immunoprecipitation, Human)
• Willumsen NJ, Amstrup J, Mobjerg N, Jespersen A, Kristensen P, Larsen EH Mitochondria-rich cells as experimental model in studies of epithelial chloride channels. Biochim. Biophys. Acta, 2002-11-13;1566(1):28-43. 2002-11-13 [PMID: 12421535] (IHC-P, Xenopus)
• Pan J, Bear C, Farragher S, Cutz E, Yeger H Cystic fibrosis transmembrane conductance regulator modulates neurosecretory function in pulmonary neuroendocrine cell-related tumor cell line models. Am. J. Respir. Cell Mol. Biol., 2002-11-01;27(5):553-60. 2002-11-01 [PMID: 12397014] (ICC, Human)
• Bagorda A, Guerra L, Di Sole F, Hemle-Kolb C, Cardone RA, Fanelli T, Reshkin SJ, Gisler SM, Murer H, Casavola V Reciprocal protein kinase A regulatory interactions between cystic fibrosis transmembrane conductance regulator and Na+/H+ exchanger isoform 3 in a renal polarized epithelial cell model. J. Biol. Chem., 2002-04-05;277(24):21480-8. 2002-04-05 [PMID: 11937500] (Western Blot, Amphibian)

Reviews for CFTR Antibody (MAB25031) (10)

Average Rating: 4.2

(Based on 10 reviews)

We have 10 reviews tested in 3 species: Human, Mouse, Other.

reviewed by: Verified Customer WB Mouse 02/19/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23060444
• Species: Mouse

reviewed by: Verified Customer IF Other 02/19/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 20107177
• Species: Other

reviewed by: Verified Customer WB Human 02/19/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 21811577
• Species: Human

reviewed by: Verified Customer WB Human 02/19/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 22904170
• Species: Human

reviewed by: Verified Customer IP Human 02/19/2015

Summary

• Application: Immunoprecipitation
• Sample Tested: See PMID 21383017
• Species: Human

reviewed by: Verified Customer IHC-Fr Mouse 02/19/2015

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: See PMID 23045527
• Species: Mouse

reviewed by: Verified Customer IF Mouse 02/19/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 24204804
• Species: Mouse

reviewed by: Verified Customer IF Mouse 02/19/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 22989883
• Species: Mouse

reviewed by: Verified Customer IF Human 02/19/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 20351101
• Species: Human

reviewed by: Verified Customer Flow Human 02/19/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 21561312
• Species: Human

Bioinformatics

• Gene Symbol: CFTR
• Uniprot:
  • P13569()