CFTR Antibody (24-1) [Unconjugated] - C-Terminus

Images

 
CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using ...read more
CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Mouse Anti-Human CFTR C‑Terminus Monoclonal Antibody (Catalog # MAB25031) overnight at 4 °C. Tissue was stained with ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clone
24-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CFTR Antibody (24-1) [Unconjugated] - C-Terminus Summary

Immunogen
Glutathione S-transferase-coupled CFTR
aa 1377-1480
Accession # P13569
Specificity
Detects in vitro synthesized CFTR and endogenous CFTR in Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CFTR
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 1-2 ug/10^6 cells
Reviewed Applications
Read 10 Reviews rated 4.2
using
MAB25031 in the following applications:

Publications
Read Publications using
MAB25031 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CFTR Antibody (24-1) [Unconjugated] - C-Terminus

  • ABC35
  • ABCC7CF
  • ATP-binding cassette sub-family C member 7
  • ATP-binding cassette transporter sub-family C member 7
  • cAMP-dependent chloride channel
  • CFTR
  • CFTR/MRP
  • Channel conductance-controlling ATPase
  • cystic fibrosis transmembrane conductance regulator (ATP-binding cassettesub-family C, member 7)
  • cystic fibrosis transmembrane conductance regulator
  • cystic fibrosis transmembrane conductance regulator, ATP-binding cassette(sub-family C, member 7)
  • dJ760C5.1
  • EC 3.6.3
  • MRP7EC 3.6.3.49
  • TNR-CFTR

Background

Cystic fibrosis transmembrane conductance regulator (CFTR) is a multi-pass transmembrane protein that functions as a chloride channel. CFTR belongs to the ATP‑binding cassette (ABC) superfamily. Mutations in CFTR cause the pulmonary disease, cystic fibrosis (CF). Specifically, deletion of phenyalanine at position 508 (DeltaF508-CFTR) results in a folding defect which impairs chloride channel function. The mechanism by which channel dysfunction relates to disease symptoms is a focus of intense research. CFTR dysfunction results in disruption of ion transport and subsequent blockage of airways by secreted mucus. CFTR may also play a role in the skeletal muscle atrophy and dysfunction that characterizes CF. In addition, CFTR-mediated chloride secretion underlies fluid accumulation and cyst growth in autosomal dominant polycystic kidney disease (ADPKD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CFTR Antibody (MAB25031)(33)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Amphibian, Fish, Fish - Goldline seabream, Ovine, Primate - Chlorocebus pygerythrus (Vervet Monkey), Xenopus.

We have publications tested in 6 applications: ICC, IHC, IHC Frozen, IHC Paraffin-embedded, IP, WB.


Filter By Application
ICC
(1)
IHC
(1)
IHC Frozen
(1)
IHC Paraffin-embedded
(2)
IP
(10)
WB
(17)
All Applications
Filter By Species
Human
(23)
Mouse
(1)
Rat
(2)
Amphibian
(1)
Fish
(2)
Fish - Goldline seabream
(1)
Ovine
(1)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using MAB25031 Applications Species
MF Figueira, RC Castiglion, CM de Lemos B, FM Ornellas, G da Silva F, MM Morales, RN da Fonseca, J de Souza-M Diabetic rats present higher urinary loss of proteins and lower renal expression of megalin, cubilin, ClC-5, and CFTR Physiol Rep, 2017;5(13):. 2017 [PMID: 28676554] (WB,, Rat) WB Rat
Z Bozoky, S Ahmadi, T Milman, TH Kim, K Du, M Di Paola, S Pasyk, R Pekhletski, JP Keller, CE Bear, JD Forman-Kay Synergy of cAMP and calcium signaling pathways in CFTR regulation Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017 [PMID: 28242698] (Human) Human
Deep interactome profiling of membrane proteins by co-interacting protein identification technology Nat Protoc, 2016;11(12):2515-2528. 2016 [PMID: 27854364] (IP,, Human) IP Human
Tabeling C, Yu H, Wang L, Ranke H, Goldenberg N, Zabini D, Noe E, Krauszman A, Gutbier B, Yin J, Schaefer M, Arenz C, Hocke A, Suttorp N, Proia R, Witzenrath M, Kuebler W CFTR and sphingolipids mediate hypoxic pulmonary vasoconstriction. Proc Natl Acad Sci U S A, 2015;112(13):E1614-23. 2015 [PMID: 25829545] (IP,, Mouse) IP Mouse
Ferru-Clement R, Fresquet F, Norez C, Metaye T, Becq F, Kitzis A, Thoreau V Involvement of the Cdc42 pathway in CFTR post-translational turnover and in its plasma membrane stability in airway epithelial cells. PLoS ONE, 2015;10(3):e0118943. 2015 [PMID: 25768293] (WB,, Human) WB Human
Caohuy H, Yang Q, Eudy Y, Ha T, Xu A, Glover M, Frizzell R, Jozwik C, Pollard H Activation of 3-phosphoinositide-dependent kinase 1 (PDK1) and serum- and glucocorticoid-induced protein kinase 1 (SGK1) by short-chain sphingolipid C4-ceramide rescues the trafficking defect of DeltaF508-cystic fibrosis transmembrane conductance regulator (DeltaF508-CFTR). J Biol Chem, 2014;289(52):35953-68. 2014 [PMID: 25384981] (IP,, Human) IP Human
Hassan F, Xu X, Nuovo G, Killilea D, Tyrrell J, Da Tan C, Tarran R, Diaz P, Jee J, Knoell D, Boyaka P, Cormet-Boyaka E Accumulation of metals in GOLD4 COPD lungs is associated with decreased CFTR levels. Respir Res, 2014;15(0):69. 2014 [PMID: 24957904] (WB,, Human) WB Human
Carbone A, Castellani S, Favia M, Diana A, Paracchini V, Di Gioia S, Seia M, Casavola V, Colombo C, Conese M Correction of defective CFTR/ENaC function and tightness of cystic fibrosis airway epithelium by amniotic mesenchymal stromal (stem) cells. J Cell Mol Med, 2014;18(8):1631-43. 2014 [PMID: 24894806] (WB,, Human) WB Human
Losa D, Kohler T, Bellec J, Dudez T, Crespin S, Bacchetta M, Boulanger P, Hong S, Morel S, Nguyen T, van Delden C, Chanson M Pseudomonas aeruginosa-induced apoptosis in airway epithelial cells is mediated by gap junctional communication in a JNK-dependent manner. J Immunol, 2014;192(10):4804-12. 2014 [PMID: 24733844] (WB,, Human) WB Human
Li Z, Lui E, Wilson J, Ip Y, Lin Q, Lam T, Lam S Expression of key ion transporters in the gill and esophageal-gastrointestinal tract of euryhaline Mozambique tilapia Oreochromis mossambicus acclimated to fresh water, seawater and hypersaline water. PLoS ONE, 2014;9(1):e87591. 2014 [PMID: 24498146] (IHC Paraffin-embedded,, Fish) IHC Paraffin-embedded Fish
Show All 33 Publications.

Reviews for CFTR Antibody (MAB25031) (10) 4.210

Average Rating: 4.2
(Based on 10 reviews)
We have 10 reviews tested in 3 species: Human, Mouse, Other.
We have 10 reviews tested in 5 application: WB, IF, IP, IHC-Fr, Flow.

Reviews using MAB25031:
Filter by Applications
WB
(3)
IF
(4)
IP
(1)
IHC-Fr
(1)
Flow
(1)
All Applications
Filter by Species
Human
(5)
Mouse
(4)
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 02/19/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23060444
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23060444

Details

Detection NotesSee PMID 23060444

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23060444
 
reviewed by:
Anonymous
IF Other 02/19/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 20107177
SpeciesOther

Blocking

Blocking DetailsSee PMID 20107177

Details

Detection NotesSee PMID 20107177

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 20107177
 
reviewed by:
Anonymous
WB Human 02/19/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21811577
SpeciesHuman

Blocking

Blocking DetailsSee PMID 21811577

Details

Detection NotesSee PMID 21811577

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 21811577
 
reviewed by:
Anonymous
WB Human 02/19/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22904170
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22904170

Details

Detection NotesSee PMID 22904170

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 22904170
 
reviewed by:
Anonymous
IP Human 02/19/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedSee PMID 21383017
SpeciesHuman

Blocking

Blocking DetailsSee PMID 21383017

Details

Detection NotesSee PMID 21383017

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 21383017
 
reviewed by:
Anonymous
IHC-Fr Mouse 02/19/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23045527
SpeciesMouse
Special ApplicationsIndependent review from antibodyresource.comPublished in PMID 23045527
 
reviewed by:
Anonymous
IF Mouse 02/19/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24204804
SpeciesMouse

Blocking

Blocking DetailsSee PMID 24204804

Details

Detection NotesSee PMID 24204804

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 24204804
 
reviewed by:
Anonymous
IF Mouse 02/19/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22989883
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22989883

Details

Detection NotesSee PMID 22989883

Comments

CommentsIndependent review from antibodyresource.comThis antibody worked well in dissociated primary airway submucosal gland cells for immunofluorescence. The staining pattern was CFTR-specific, determined using knockout mouse tissue and blocking antigenic peptides (Published in PMID: 22989883).
 
reviewed by:
Anonymous
IF Human 02/19/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 20351101
SpeciesHuman

Blocking

Blocking DetailsSee PMID 20351101

Details

Detection NotesSee PMID 20351101

Comments

CommentsIndependent review from antibodyresource.comThe antibody was also used for WB analysis (Published in PMID 20351101).
 
reviewed by:
Anonymous
Flow Human 02/19/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 21561312
SpeciesHuman

Blocking

Blocking DetailsSee PMID 21561312

Details

Detection NotesSee PMID 21561312

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 21561312

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CFTR Antibody (MAB25031) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CFTR Antibody (MAB25031)

Discover related pathways, diseases and genes to CFTR Antibody (MAB25031). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CFTR Antibody (MAB25031)

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Blogs on CFTR.

The ABCA1 Antibody and ABC Transporter Research
The ABC (ATP-binding cassette) transporters are a superfamily of transmembrane proteins which play an essential role in membrane transport in both prokaryotes and eukaryotes. In humans, ABC transporters have been implicated in many conditions. For exa...  Read full blog post.

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Recent Reviews

4.2
5
2
4
8
3
0
2
0
1
0

 
Anonymous
02/19/2015
Application: WB
Species: Mouse

 
Anonymous
02/19/2015
Application: IF
Species: Other

 
Anonymous
02/19/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol CFTR
Entrez
Uniprot