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CtIP Antibody

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Immunoprecipitation: CtIP Antibody [NB100-79809] - Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded) from MCF7 cells. Antibodies: Affinity purified rabbit anti-CtIP antibody BL1913 (NB100-79809) used at 3 ug/mg lysate for IP. CtIP was also immunoprecipitated with rabbit anti-CtIP antibody BL1914 (NB100-79810) using 3 ug/mg lysate. Immunoprecipitated CtIP was blotted using BL1914 at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: CtIP Antibody [NB100-79809] - Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded) from MCF7 cells. Antibodies: Affinity purified rabbit anti-CtIP antibody BL1913 (NB100-79809) used at 3 ug/mg ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IP, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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CtIP Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 525 and 575 of human CtBP Interacting Protein (Retinoblastoma Binding Protein 8) using the numbering given in entry NP_002885.1 (GeneID 5932).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RBBP8
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 2-5 ug/mg lysate
  • Chromatin Immunoprecipitation (ChIP)
Application Notes
Use in chromatin immunoprecipitation reported in scientific literature (PMID 29244158).
Publications
Read Publications using
NB100-79809 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CtIP Antibody

  • CtBP-interacting protein
  • CtIP
  • DNA endonuclease RBBP8
  • EC 3.1
  • RBBP-8
  • retinoblastoma binding protein 8
  • Retinoblastoma-binding protein 8
  • Retinoblastoma-interacting protein and myosin-like
  • RIMSAE2
  • Sporulation in the absence of SPO11 protein 2 homolog

Background

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: BRCA1 Antibody (RAY) [NB100-598] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using BRCA1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
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Western Blot: BRIP1/FANCJ Antibody [NB100-416] - BRIP1/FANCJ detected in cell lysates using NB 100-416. Lane 1: MCF-7 lysate, Lane 2: HeLa lysate, Lane 3: 293 lysate and Lane 4: SKOV3 lysate.
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Enzyme E1 (SAE1) Anti ...
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cleaved
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Immunocytochemistry/Immunofluorescence: UNC13B Antibody [NBP1-33037] - Analysis of methanol-fixed HeLa, using UNC13B antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.
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Publications for CtIP Antibody (NB100-79809)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ChIP.


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Bioinformatics

Gene Symbol RBBP8
Entrez
Uniprot