CtIP Antibody

Immunoprecipitation: CtIP Antibody [NB100-79809] - Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded) from MCF7 cells. Antibodies: Affinity purified rabbit anti-CtIP antibody BL1913 (NB100-79809) used at 3 ug/mg lysate for IP. CtIP was also immunoprecipitated with rabbit anti-CtIP antibody BL1914 (NB100-79810) using 3 ug/mg lysate. Immunoprecipitated CtIP was blotted using BL1914 at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu
• Applications: ICC/IF, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

CtIP Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 525 and 575 of human CtBP Interacting Protein (Retinoblastoma Binding Protein 8) using the numbering given in entry NP_002885.1 (GeneID 5932).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBBP8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 2-5 ug/mg lysate
• Application Notes: Use in chromatin immunoprecipitation reported in scientific literature (PMID 29244158). Use in ICC/IF reported in scientific literature (PMID: 32401820).

Reactivity Notes

Use in Mouse reported in scientific publication (PMID: 32401820).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CtIP Antibody

• CtBP-interacting protein
• CtIP
• DNA endonuclease RBBP8
• EC 3.1
• RBBP-8
• retinoblastoma binding protein 8
• Retinoblastoma-binding protein 8
• Retinoblastoma-interacting protein and myosin-like
• RIMSAE2
• Sporulation in the absence of SPO11 protein 2 homolog

Background

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CtIP Antibody (NB100-79809)(5)

Publications using NB100-79809

• Song S, Creus Muncunill J, Galicia Aguirre C et al. Postnatal Conditional Deletion of Bcl11b in Striatal Projection Neurons Mimics the Transcriptional Signature of Huntington's Disease Biomedicines 2022-09-23 [PMID: 36289639]
• Shi Y, Sun L, Wang M et al. Vascularized human cortical organoids (vOrganoids) model cortical development in vivo PLoS Biol. 2020-05-01 [PMID: 32401820] (ICC/IF, Mouse)
• Chen G, Chen J, Qiao Y et al. ZNF830 mediates cancer chemoresistance through promoting homologous-recombination repair. Nucleic Acids Res. 2017-12-13 [PMID: 29244158] (Chemotaxis, Human)
• Liu J, Liu W, Yang L et al. The Primate-Specific Gene TMEM14B Marks Outer Radial Glia Cells and Promotes Cortical Expansion and Folding. Cell Stem Cell. 2017-10-10 [PMID: 29033352] (Human)
• Yin Y, Seifert A, Chua JS et al. SUMO-targeted ubiquitin E3 ligase RNF4 is required for the response of human cells to DNA damage. Genes Dev 2012-06-01 [PMID: 22661230]

Bioinformatics

• Gene Symbol: RBBP8
• Entrez:
  • 5932(Human)
• Uniprot:
  • NP_002885.1(Human)