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MDC1 Antibody

Images

 
Western Blot: MDC1 Antibody [NB100-395] - Human lung cancer cell lines (UT) Untreated and (Cis) 10uM Cisplatin 16h. Image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: MDC1 Antibody [NB100-395] - PARP activity affects 53BP1 recruitment to low-input power damage. Immunofluorescent localization of MDC1 and 53BP1 in HeLa cells irradiated with 15% ...read more
Western Blot: MDC1 Antibody [NB100-395] - Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MDC1 antibody NB100-395 used for WB at 0.1 ...read more
Immunoprecipitation: MDC1 Antibody [NB100-395] - Detection of human MDC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity ...read more
BRUCE UBC domain is required for NBS1 repair foci formation.U2OS-shBRUCE cells with stable expression of BRUCE or BRUCE mutants as indicated to the left were treated with DOX to knockdown endogenous BRUCE. After ...read more

Product Details

Summary
Reactivity Hu, Mu, Pm, PmSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

MDC1 Antibody Summary

Immunogen
A synthetic peptide, which represented a portion of human Mediator of DNA Damage Checkpoint Protein 1 encoded within exon 5 (NP_055456.1).
Predicted Species
Monkey (100%), Chimpanzee (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MDC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:250
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Western Blot 1:2000 - 1:10000
Application Notes
WB of lysates performed using standard western blot reagents and 4-8% SDS-PAGE. ICC/IF reactivity reported in (PMID: 26903517). MDC1 antibody validated for WB from a verified customer review.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-395 in the following applications:

Publications
Read Publications using
NB100-395 in the following applications:

  • 6 publications
  • WB
    4 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26903517).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MDC1 Antibody

  • DKFZp781A0122
  • Em:AB023051.5
  • KIAA0170MGC166888
  • MDC1
  • mediator of DNA damage checkpoint 1
  • mediator of DNA damage checkpoint protein 1
  • mediator of DNA-damage checkpoint 1
  • NFBD1
  • NFBD1homologue to Drosophila photoreceptor protein calphotin
  • Nuclear factor with BRCT domains 1

Background

MDC1 works with H2AX to promote recruitment of repair proteins to the sites of DNA breaks and controls damage-induced cell-cycle arrest checkpoints. Cells that lack MDC1 are sensitive to ionizing radiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-395
Species: Hu, Mu, Pm, Pm
Applications: WB, ICC/IF, IP

Publications for MDC1 Antibody (NB100-395)(19)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(6)
WB
(4)
All Applications
Filter By Species
Human
(5)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NB100-395 Applications Species
Heyza JR, Mikhova M, Bahl A et al. Systematic analysis of the molecular and biophysical properties of key DNA damage response factors eLife 2023-06-21 [PMID: 37341699]
Schleicher EM, Dhoonmoon A, Jackson LM et al. The TIP60-ATM axis regulates replication fork stability in BRCA-deficient cells Oncogenesis 2022-06-18 [PMID: 35717336] (WB, Human) WB Human
Walter L, Canup B, Pujada A et al. Matrix metalloproteinase 9 (MMP9) limits reactive oxygen species (ROS) accumulation and DNA damage in colitis-associated cancer Cell Death Dis 2020-09-17 [PMID: 32943603] (WB, Mouse) WB Mouse
Bick G, Zhang F, Meetei AR, Andreassen PR. Coordination of the recruitment of the FANCD2 and PALB2 Fanconi anemia proteins by an ubiquitin signaling network. Chromosoma 2016-06-08 [PMID: 27277787]
Hodge CD, Ismail IH, Edwards RA et al. RNF8 E3 Ubiquitin Ligase Stimulates Ubc13 E2 Conjugating Activity That Is Essential for DNA Double Strand Break Signaling and BRCA1 Tumor Suppressor Recruitment. J Biol Chem 2016-04-29 [PMID: 26903517] (ICC/IF, Mouse) ICC/IF Mouse
Ge C, Che L, Du C. The UBC Domain Is Required for BRUCE to Promote BRIT1/MCPH1 Function in DSB Signaling and Repair Post Formation of BRUCE-USP8-BRIT1 Complex. PLoS ONE. 2015-12-19 [PMID: 26683461] (WB, ICC/IF, Human)

Details:
MDC1 antibody was used for ICC/IF analysis of MDC1 in experiments involving U2OS-shBRUCE cells with stable expression of BRUCE or BRUCE mutants (Fig 6A). The antibody was also used for WB on DOX treated shBRUCE-U2OS cells which were irradiated before preparation of lysates (Fig 6C).
WB, ICC/IF Human
Saquilabon Cruz GM, Kong X, Silva BA et al. Femtosecond near-infrared laser microirradiation reveals a crucial role for PARP signaling on factor assemblies at DNA damage sites Nucleic Acids Res 2016-02-18 [PMID: 26424850] (ICC/IF)

Details:
Potorous tridactylis (Long-nosed Potoroo)
ICC/IF
Samba-Louaka A, Pereira Jm, Nahori Ma et al. Listeria monocytogenes Dampens the DnA Damage Response. PLoS Pathog. 2014-10-01 [PMID: 25340842] (ICC/IF, Human) ICC/IF Human
Silva Barbara Alcaraz, Stambaugh Jessica R, Yokomori Kyoko et al. DNA damage to a single chromosome end delays anaphase onset. J Biol Chem. 2014-08-15 [PMID: 24982423]
Munch Sandra, Weidtkamp-Peters Stefanie, Klement Karolin et al. The tumor suppressor PML specifically accumulates at RPA/Rad51-containing DNA damage repair foci but is nonessential for DNA damage-induced fibroblast senescence. Mol Cell Biol. 2014-05-01 [PMID: 24615016]
Show All 19 Publications.

Reviews for MDC1 Antibody (NB100-395) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.

Reviews using NB100-395:
Filter by Applications
WB
(1)
IF
(1)
All Applications
Filter by Species
Human
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot MDC1 NB100-395
Enlarge
4
reviewed by:
Verified Customer
WB Human 02/18/2019
View

Summary

ApplicationWestern Blot
Sample Testedhuman lung cancer cell line
SpeciesHuman
LotA3
  5
reviewed by:
Verified Customer
IF Other 10/19/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMarsupial
SpeciesOther

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MDC1 Antibody (NB100-395) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional MDC1 Products

Array NB100-395

Research Areas for MDC1 Antibody (NB100-395)

Find related products by research area.

Blogs on MDC1.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
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0
2
0
1
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Verified Customer
02/18/2019
Application: WB
Species: Human

 
Verified Customer
10/19/2015
Application: IF
Species: Other

Bioinformatics

Gene Symbol MDC1
Entrez
Uniprot