MDC1 Antibody

Images

 
Western Blot: MDC1 Antibody [NB100-395] - Detection of Human MDC1 by Western Blot.Samples: A) Nuclear extract (50 mcg) from HeLa cells. B) Whole cell lysate from HEK 293 cells. Antibodies: A) Affinity purified rabbit ...read more
Proximity Ligation Assay: MDC1 Antibody [NB100-395] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMC1 (NBP2-14858) and rabbit anti-human MDC1 (NB100-395). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: MDC1 Antibody [NB100-395] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human MDC1 (NB100-395). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: MDC1 Antibody [NB100-395] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human DMAP1 (NB100-256) and rabbit anti-human MDC1 (NB100-395). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: MDC1 Antibody [NB100-395] - Secondary-conjugate Duolink In Situ PLA in MCF-7. goat anti-human POLR2A (NBP2-14859) and rabbit anti-human MDC1 (NB100-395). Image merged from DAPI (2ms), ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, PLA, IP (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

       

MDC1 Antibody Summary

Immunogen
A synthetic peptide, which represented a portion of human Mediator of DNA Damage Checkpoint Protein 1 encoded within exon 5 (NP_055456.1).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MDC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2500
  • Immunocytochemistry/Immunofluorescence 1:100-1:250
  • Proximity Ligation Assay 1:1000-1:10000
Application Notes
This antibody may be used for Western Blot, where a doublet band should be seen at ~250 kDa. It may also be used for immunofluorescence. Immunoprecipitation is not recommended for this antibody** **NB 100-396 and NB 100-397 are recommended for IP.
Reviewed Applications
Read 1 Review rated 5
using
NB100-395 in the following applications:

Publications
Read Publications using
NB100-395 in the following applications:

  • 6 publications
  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26903517). Based on 100% sequence identity, this antibody is predicted to react with Gorilla and Chimpanzee.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MDC1 Antibody

  • DKFZp781A0122
  • Em:AB023051.5
  • KIAA0170MGC166888
  • MDC1
  • mediator of DNA damage checkpoint 1
  • mediator of DNA damage checkpoint protein 1
  • mediator of DNA-damage checkpoint 1
  • NFBD1
  • NFBD1homologue to Drosophila photoreceptor protein calphotin
  • Nuclear factor with BRCT domains 1

Background

MDC1 works with H2AX to promote recruitment of repair proteins to the sites of DNA breaks and controls damage-induced cell-cycle arrest checkpoints. Cells that lack MDC1 are sensitive to ionizing radiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-304
Species: Hu, Mu, Rt, Fi, Gt, Pm
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO
NB100-384
Species: Hu, Mu, Rt, Ca
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
NB100-104
Species: Hu, Mu
Applications: WB, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP
NB100-143
Species: Hu, Mu, Ha, Ma, Pm
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-P, IP, KO
NB100-598
Species: Hu
Applications: WB, Flow, ICC/IF, IP, CyTOF-ready
NB100-56546
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB100-142
Species: Hu, Mu, Rt, Ch, Ha
Applications: WB, Simple Western, ChIP, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB100-56155
Species: Hu
Applications: WB, IHC, IHC-P, IP
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-308
Species: Hu
Applications: WB, Func, ICC/IF, IP
AF2396
Species: Hu
Applications: WB, Simple Western, IHC
NBP2-03417
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NB100-56585
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF1085
Species: Mu
Applications: WB, IHC, Neut
NB100-464
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, PLA
H00009025-B01P
Species: Hu
Applications: WB, ICC/IF
NB100-147
Species: Hu, Mu, Rt, Mk
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-22128
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC-P

Publications for MDC1 Antibody (NB100-395)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(6)
WB
(2)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB100-395 Applications Species
Bick G, Zhang F, Meetei AR, Andreassen PR. Coordination of the recruitment of the FANCD2 and PALB2 Fanconi anemia proteins by an ubiquitin signaling network. Chromosoma Jun 8 2016 [PMID: 27277787]
Hodge CD, Ismail IH, Edwards RA et al. RNF8 E3 Ubiquitin Ligase Stimulates Ubc13 E2 Conjugating Activity that is Essential for DNA Double-Strand Break Signaling and BRCA1 Tumor Suppressor Recruitment. J. Biol. Chem. 2016 Feb 22 [PMID: 26903517] (ICC/IF, Mouse) ICC/IF Mouse
Ge C, Che L, Du C. The UBC Domain Is Required for BRUCE to Promote BRIT1/MCPH1 Function in DSB Signaling and Repair Post Formation of BRUCE-USP8-BRIT1 Complex. PLoS ONE. 2015 Dec 19 [PMID: 26683461] (ICC/IF, WB, Human)

Details:
MDC1 antibody was used for ICC/IF analysis of MDC1 in experiments involving U2OS-shBRUCE cells with stable expression of BRUCE or BRUCE mutants (Fig 6A). The antibody was also used for WB on DOX treated shBRUCE-U2OS cells which were irradiated before preparation of lysates (Fig 6C).
ICC/IF, WB Human
Saquilabon Cruz GM, Kong X, Silva BA et al. Femtosecond near-infrared laser microirradiation reveals a crucial role for PARP signaling on factor assemblies at DNA damage sites. Nucleic Acids Res 2016 Feb 18 [PMID: 26424850] (ICC/IF) ICC/IF
Samba-Louaka A, Pereira Jm, Nahori Ma et al. Listeria monocytogenes Dampens the DnA Damage Response. PLoS Pathog. 2014 Oct 01 [PMID: 25340842] (ICC/IF, Human) ICC/IF Human
Silva Barbara Alcaraz, Stambaugh Jessica R, Yokomori Kyoko et al. DNA damage to a single chromosome end delays anaphase onset. J Biol Chem. 2014 Aug 15 [PMID: 24982423]
Munch Sandra, Weidtkamp-Peters Stefanie, Klement Karolin et al. The tumor suppressor PML specifically accumulates at RPA/Rad51-containing DNA damage repair foci but is nonessential for DNA damage-induced fibroblast senescence. Mol Cell Biol. 2014 May 01 [PMID: 24615016]
Mok MT, Henderson BR. The in vivo dynamic interplay of MDC1 and 53BP1 at DNA damage-induced nuclear foci. Int J Biochem Cell Biol 2012 Sep [PMID: 22677490]
Siddiqui-Jain A, Bliesath J, Macalino D et al. CK2 inhibitor CX-4945 suppresses DNA repair response triggered by DNA-targeted anticancer drugs and augments efficacy: mechanistic rationale for drug combination therapy. Mol Cancer Ther 2012 Apr [PMID: 22267551]
Reichert S, Rodel C, Mirsch J, Harter PN, Tomicic MT, Mittelbronn M, Kaina B, Rodel F. Survivin inhibition and DNA double-strand break repair: a molecular mechanism to overcome radioresistance in glioblastoma. Radiother Oncol;101(1):51-8. 2011 Oct. [PMID: 21852011] (WB, ICC/IF, Human) WB, ICC/IF Human
Show All 16 Publications.

Review for MDC1 Antibody (NB100-395) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: IF.

Reviews using NB100-395:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Other 10/19/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMarsupial
SpeciesOther

Blocking

Blocking DetailsBSA

Details

Detection NotesyH2AX used as a positive control
Fixation Details4% PFA

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MDC1 Antibody (NB100-395) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional MDC1 Products

MDC1 NB100-395

Bioinformatics Tool for MDC1 Antibody (NB100-395)

Discover related pathways, diseases and genes to MDC1 Antibody (NB100-395). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MDC1 Antibody (NB100-395)

Discover more about diseases related to MDC1 Antibody (NB100-395).
 

Pathways for MDC1 Antibody (NB100-395)

View related products by pathway.

PTMs for MDC1 Antibody (NB100-395)

Learn more about PTMs related to MDC1 Antibody (NB100-395).
 

Research Areas for MDC1 Antibody (NB100-395)

Find related products by research area.

Blogs on MDC1.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Anonymous
10/19/2015
Application: IF
Species: Other

Bioinformatics

Gene Symbol MDC1
Entrez
Uniprot