MDC1 Antibody

Images

 
Western Blot: MDC1 Antibody [NB100-395] - Human lung cancer cell lines (UT) Untreated and (Cis) 10uM Cisplatin 16h. Image from verified customer review.
Western Blot: MDC1 Antibody [NB100-395] - Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MDC1 antibody NB100-395 used for WB at 0.1 ...read more
Immunoprecipitation: MDC1 Antibody [NB100-395] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MDC1 antibody NB100-395 used for IP at 3 ...read more
Independent Antibodies: MDC1 Antibody [NB100-395] - Detection of human MDC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. ...read more

Product Details

Summary
Reactivity Hu, Mu, Mk, PmSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

MDC1 Antibody Summary

Immunogen
A synthetic peptide, which represented a portion of human Mediator of DNA Damage Checkpoint Protein 1 encoded within exon 5 (NP_055456.1).
Predicted Species
Primate (100%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MDC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2500
  • Immunocytochemistry/Immunofluorescence 1:100-1:250
  • Immunoprecipitation 2 - 5 ug/mg lysate
Application Notes
WB of lysates performed using standard western blot reagents and 4-8% SDS-PAGE. ICC/IF reactivity reported in (PMID: 26903517).
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-395 in the following applications:

Publications
Read Publications using
NB100-395 in the following applications:

  • 6 publications
  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26903517). Chimpanzee (100%).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MDC1 Antibody

  • DKFZp781A0122
  • Em:AB023051.5
  • KIAA0170MGC166888
  • MDC1
  • mediator of DNA damage checkpoint 1
  • mediator of DNA damage checkpoint protein 1
  • mediator of DNA-damage checkpoint 1
  • NFBD1
  • NFBD1homologue to Drosophila photoreceptor protein calphotin
  • Nuclear factor with BRCT domains 1

Background

MDC1 works with H2AX to promote recruitment of repair proteins to the sites of DNA breaks and controls damage-induced cell-cycle arrest checkpoints. Cells that lack MDC1 are sensitive to ionizing radiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-395
Species: Hu, Mu, Mk, Pm
Applications: WB, ICC/IF, IP

Publications for MDC1 Antibody (NB100-395)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(6)
WB
(2)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB100-395 Applications Species
Bick G, Zhang F, Meetei AR, Andreassen PR. Coordination of the recruitment of the FANCD2 and PALB2 Fanconi anemia proteins by an ubiquitin signaling network. Chromosoma Jun 8 2016 [PMID: 27277787]
Hodge CD, Ismail IH, Edwards RA et al. RNF8 E3 Ubiquitin Ligase Stimulates Ubc13 E2 Conjugating Activity that is Essential for DNA Double-Strand Break Signaling and BRCA1 Tumor Suppressor Recruitment. J. Biol. Chem. 2016 Feb 22 [PMID: 26903517] (ICC/IF, Mouse) ICC/IF Mouse
Ge C, Che L, Du C. The UBC Domain Is Required for BRUCE to Promote BRIT1/MCPH1 Function in DSB Signaling and Repair Post Formation of BRUCE-USP8-BRIT1 Complex. PLoS ONE. 2015 Dec 19 [PMID: 26683461] (ICC/IF, WB, Human)

Details:
MDC1 antibody was used for ICC/IF analysis of MDC1 in experiments involving U2OS-shBRUCE cells with stable expression of BRUCE or BRUCE mutants (Fig 6A). The antibody was also used for WB on DOX treated shBRUCE-U2OS cells which were irradiated before preparation of lysates (Fig 6C).
ICC/IF, WB Human
Saquilabon Cruz GM, Kong X, Silva BA et al. Femtosecond near-infrared laser microirradiation reveals a crucial role for PARP signaling on factor assemblies at DNA damage sites. Nucleic Acids Res 2016 Feb 18 [PMID: 26424850] (ICC/IF) ICC/IF
Samba-Louaka A, Pereira Jm, Nahori Ma et al. Listeria monocytogenes Dampens the DnA Damage Response. PLoS Pathog. 2014 Oct 01 [PMID: 25340842] (ICC/IF, Human) ICC/IF Human
Silva Barbara Alcaraz, Stambaugh Jessica R, Yokomori Kyoko et al. DNA damage to a single chromosome end delays anaphase onset. J Biol Chem. 2014 Aug 15 [PMID: 24982423]
Munch Sandra, Weidtkamp-Peters Stefanie, Klement Karolin et al. The tumor suppressor PML specifically accumulates at RPA/Rad51-containing DNA damage repair foci but is nonessential for DNA damage-induced fibroblast senescence. Mol Cell Biol. 2014 May 01 [PMID: 24615016]
Mok MT, Henderson BR. The in vivo dynamic interplay of MDC1 and 53BP1 at DNA damage-induced nuclear foci. Int J Biochem Cell Biol 2012 Sep [PMID: 22677490]
Siddiqui-Jain A, Bliesath J, Macalino D et al. CK2 inhibitor CX-4945 suppresses DNA repair response triggered by DNA-targeted anticancer drugs and augments efficacy: mechanistic rationale for drug combination therapy. Mol Cancer Ther 2012 Apr [PMID: 22267551]
Reichert S, Rodel C, Mirsch J, Harter PN, Tomicic MT, Mittelbronn M, Kaina B, Rodel F. Survivin inhibition and DNA double-strand break repair: a molecular mechanism to overcome radioresistance in glioblastoma. Radiother Oncol;101(1):51-8. 2011 Oct. [PMID: 21852011] (WB, ICC/IF, Human) WB, ICC/IF Human
Show All 16 Publications.

Reviews for MDC1 Antibody (NB100-395) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.

Reviews using NB100-395:
Filter by Applications
WB
(1)
IF
(1)
All Applications
Filter by Species
Human
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot MDC1 NB100-395
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reviewed by:
Anonymous
WB Human 02/18/2019
View

Summary

ApplicationWestern Blot
Sample Testedhuman lung cancer cell line
SpeciesHuman
LotA3
 
reviewed by:
Anonymous
IF Other 10/19/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMarsupial
SpeciesOther

Blocking

Blocking DetailsBSA

Details

Detection NotesyH2AX used as a positive control
Fixation Details4% PFA

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MDC1 Antibody (NB100-395) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-395AF350
Alexa Fluor 405 NB100-395AF405
Alexa Fluor 488 NB100-395AF488
Alexa Fluor 532 NB100-395AF532
Alexa Fluor 594 NB100-395AF594
Alexa Fluor 647 NB100-395AF647
Alexa Fluor 700 NB100-395AF700
Alexa Fluor 750 NB100-395AF750
Biotin NB100-395B
DyLight 350 NB100-395UV
DyLight 405 NB100-395V
DyLight 488 NB100-395G
DyLight 550 NB100-395R
DyLight 594 NB100-395DL594
DyLight 650 NB100-395C
DyLight 680 NB100-395FR
DyLight 755 NB100-395IR
FITC NB100-395F
HRP NB100-395H
Janelia Fluor 549 NB100-395JF549
Janelia Fluor 646 NB100-395JF646

Additional MDC1 Products

Array NB100-395

Bioinformatics Tool for MDC1 Antibody (NB100-395)

Discover related pathways, diseases and genes to MDC1 Antibody (NB100-395). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for MDC1 Antibody (NB100-395)

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Pathways for MDC1 Antibody (NB100-395)

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PTMs for MDC1 Antibody (NB100-395)

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Research Areas for MDC1 Antibody (NB100-395)

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Blogs on MDC1.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Anonymous
02/18/2019
Application: WB
Species: Human

 
Anonymous
10/19/2015
Application: IF
Species: Other

Bioinformatics

Gene Symbol MDC1
Entrez
Uniprot