Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection in TEM8 transfected cell lysate using this antibody.
Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection of TEM8/ANTXR1 in human liver cancer section using 5 ug/ml concentration of TEM8 antibody. Uniform weak staining was ...read more
Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Analysis of human heart lysates (35ug per lane, RIPA buffer) using TEM8 antibody (NB100-56585) at 0.3ug/ml. Band observed at 65kDa. (Expected MW of ...read more
Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Human colon stained with TEM8 antibody at 2.5 ug/ml concentration using DAB chromogen detection method (10X magnification)
This monoclonal antibody was developed by immunizing mice with a synthetic peptide containing amino acids 481-497 (CINFTRVKNNQPAKYPL) of human TEM8.
TEM8 is observed at ~80 kDa in western blots of HUVEC cells (Hotchkiss et al, 2005) and as an 80 kDa and 85 kDa double in human colon cell lysates (Nanada et al, 2004). The size difference is thought to be due to glycosylation as a 70 kDa band was seen when cell extracts in Nanda et al (2004) were treated with a glycosidase cocktail. Nanda et al (2004) note a passage dependent loss of TEM8 in HUVEC suggesting that that TEM8 may be a specific marker of the endothelial cell (EC) phenotype which is lost as primary EC's age in culture and de-differentiate.
Protein G purified
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Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Alternate Names for TEM8/ANTXR1 Antibody (200C1339(SB20)) - BSA Free
anthrax toxin receptor 1
Tumor endothelial marker 8
Recently, using SAGE (Serial Analysis of Gene Expression) technology, St. Croix et al, have identified 46 genes, whose expression is specifically elevated in tumor-associated endothelium. Nine of these genes were prominently expressed only in tumor endothelial cells (EC), but were absent or barely detectable in normal ECs, and named as Tumor Endothelial Markers (TEMs, TEM 1-9). Based on TEM8s interaction with the C5 domain of collagen a3(VI), a protein also preferentially expressed in tumor endothelium, it is thought that TEM8 plays an important role in tumor angiogenesis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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