TEM8/ANTXR1 Antibody (200C1339(SB20))

Images

 
Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection in TEM8 transfected cell lysate using this antibody.
Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection of TEM8/ANTXR1 in human liver cancer section using 5 ug/ml concentration of TEM8 antibody. Uniform weak staining was ...read more
Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Analysis of human heart lysates (35ug per lane, RIPA buffer) using TEM8 antibody (NB100-56585) at 0.3ug/ml. Band observed at 65kDa. (Expected MW of ...read more
Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Formalin-fixed paraffin-embedded section of human colon stained with TEM8 antibody at 2.5 ug/ml concentration using DAB chromogen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
200C1339(SB20)
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

TEM8/ANTXR1 Antibody (200C1339(SB20)) Summary

Immunogen
This monoclonal antibody was developed by immunizing mice with a synthetic peptide containing amino acids 481-497 (CINFTRVKNNQPAKYPL) of human TEM8.
Specificity
TEM8 is observed at ~80 kDa in western blots of HUVEC cells (Hotchkiss et al, 2005) and as an 80 kDa and 85 kDa double in human colon cell lysates (Nanada et al, 2004). The size difference is thought to be due to glycosylation as a 70 kDa band was seen when cell extracts in Nanda et al (2004) were treated with a glycosidase cocktail. Nanda et al (2004) note a passage dependent loss of TEM8 in HUVEC suggesting that that TEM8 may be a specific marker of the endothelial cell (EC) phenotype which is lost as primary EC's age in culture and de-differentiate.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ANTXR1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 2-5 ug/ml
  • Immunohistochemistry-Paraffin 2-5 ug/ml
Application Notes
Immunohistochemistry-Paraffin: see Hotchkiss et al 2005 for details. Immunocytochemistry/Immunofluorescence: See Matsuda (2010) for details.
Publications
Read Publications using
NB100-56585 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for TEM8/ANTXR1 Antibody (200C1339(SB20))

  • anthrax toxin receptor 1
  • ANTXR1
  • ATR2310008J16Rik
  • FLJ10601
  • FLJ11298
  • FLJ21776
  • TEM8
  • TEM82810405N18Rik
  • Tumor endothelial marker 8

Background

Recently, using SAGE (Serial Analysis of Gene Expression) technology, St. Croix et al, have identified 46 genes, whose expression is specifically elevated in tumor-associated endothelium. Nine of these genes were prominently expressed only in tumor endothelial cells (EC), but were absent or barely detectable in normal ECs, and named as Tumor Endothelial Markers (TEMs, TEM 1-9). Based on TEM8s interaction with the C5 domain of collagen a3(VI), a protein also preferentially expressed in tumor endothelium, it is thought that TEM8 plays an important role in tumor angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for TEM8/ANTXR1 Antibody (NB100-56585)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
WB
(3)
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Filter By Species
Human
(3)
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Showing Publications 1 - 4 of 4.
Publications using NB100-56585 Applications Species
Matsuda K, Ohga N, Hida Y et al. Isolated tumor endothelial cells maintain specific character during long-term culture. Biochem Biophys Res Commun. 2010 Apr 16 [PMID: 20302845] (ICC/IF, Human)

Details:
IF/ICC: primary human xenograft tumor endothelial cell cultures (Fig 2B).
ICC/IF Human
Werner E, Kowalczyk AP, Faundez V. Anthrax toxin receptor 1/tumor endothelium marker 8 mediates cell spreading by coupling extracellular ligands to the actin cytoskeleton. J Biol Chem. 2006 Aug 11 [PMID: 16762926] (WB)

Details:
WB: TEM8 transfected/non-transfected 293 cells (Figs 1A, 4A, and 5D). Note: antibody specificity is validated by using TEM8 transfected/non-transfected 293 cells in Figs 1A and 4A.
WB
Hotchkiss KA, Basile CM, Spring SC et al. TEM8 expression stimulates endothelial cell adhesion and migration by regulating cell-matrix interactions on collagen. Exp Cell Res. 2005 Apr 15 [PMID: 15777794] (WB, IHC-P, Human)

Details:
IHC (Paraffin): Fig 1A (human umbilical cords); WB: Fig 1C and 1D [human venous epithelial cells (HUVEC)].
WB, IHC-P Human
Nanda A, Carson-Walter EB, Seaman S et al. TEM8 interacts with the cleaved C5 domain of collagen alpha 3(VI). Cancer Res. 2004 Feb 1 [PMID: 14871805] (WB, Human)

Details:
WB: TEM8 transfected/non-transfected 293 cells (Fig 1A and B), human colon cancer tissue lysates and patient-matched normal colonic mucosa transfected (Fig 1C). Note: antibody specificity is validated by using TEM8 transfected/non-transfected 293 cells in
WB Human

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56585AF405
Alexa Fluor 488 Labeled NB100-56585AF488
Alexa Fluor 647 Labeled NB100-56585AF647
Alexa Fluor 700 Labeled NB100-56585AF700
Biotin Labeled NB100-56585B
DyLight 350 Labeled NB100-56585UV
DyLight 405 Labeled NB100-56585V
DyLight 488 Labeled NB100-56585G
DyLight 550 Labeled NB100-56585R
DyLight 650 Labeled NB100-56585C
DyLight 680 Labeled NB100-56585FR
DyLight 755 Labeled NB100-56585IR
HRP Labeled NB100-56585H

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Bioinformatics

Gene Symbol ANTXR1
Entrez
Uniprot