BRCA2 Antibody (234403)

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Western blot shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.2 µg/mL of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
234403
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

BRCA2 Antibody (234403) Summary

Immunogen
E. coli-derived recombinant human BRCA2
Met1-Thr200
Accession # P51587
Specificity
Detects endogenous human BRCA2.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BRCA2
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publications using
MAB2476 in the following applications:

  • WB
    6 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BRCA2 Antibody (234403)

  • BRCA1/BRCA2-containing complex, subunit 2
  • BRCA2
  • BRCC2
  • breast and ovarian cancer susceptibility gene, early onset
  • breast cancer 2 tumor suppressor
  • breast cancer 2, early onset
  • breast cancer susceptibility protein BRCA2
  • breast cancer type 2 susceptibility protein
  • BROVCA2
  • FACD
  • FAD
  • FAD1
  • FANCB
  • FANCD
  • FANCD1Fanconi anemia, complementation group D1
  • Fanconi anemia group D1 protein
  • GLM3
  • PNCA2

Background

BRCA2 is a nuclear protein that acts as a tumor suppressor. Germline mutation of BRCA2 accounts for many cases of familial breast and ovarian cancer. At the cellular level, BRCA2 is involved in homologous recombination (HR), a form of double-strand break repair. BRCA2 interacts with the Rad51 recombinase, another protein critical for HR, to preserve chromosomal integrity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BRCA2 Antibody (MAB2476)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(6)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 6 of 6.
Publications using MAB2476 Applications Species
J Im, J Lawrence, D Seelig, RS Nho FoxM1-dependent RAD51 and BRCA2 signaling protects idiopathic pulmonary fibrosis fibroblasts from radiation-induced cell death Cell Death Dis, 2018;9(6):584. 2018 [PMID: 29789556] (WB,, Human) WB Human
S Maifrede, K Martin, P Podszywalo, K Sullivan, SK Langer, R Nejadi, Y Dasgupta, M Hulse, D Gritsyuk, M Nieborowsk, LN Lupey-Gree, H Zhao, K Piwocka, MA Wasik, I Tempera, T Skorski IGH/MYC Translocation Associates with BRCA2 Deficiency and Synthetic Lethality to PARP1 Inhibitors Mol. Cancer Res., 2017;0(0):. 2017 [PMID: 28634224] (WB,, Human) WB Human
M Nieborowsk, K Sullivan, Y Dasgupta, P Podszywalo, G Hoser, S Maifrede, E Martinez, D Di Marcant, E Bolton-Gil, K Cramer-Mor, J Lee, M Li, A Slupianek, D Gritsyuk, S Cerny-Reit, I Seferynska, T Stoklosa, L Bullinger, H Zhao, V Gorbunova, K Piwocka, P Valent, CI Civin, M Muschen, JE Dick, JC Wang, S Bhatia, R Bhatia, K Eppert, MD Minden, SM Sykes, T Skorski Gene expression and mutation-guided synthetic lethality eradicates proliferating and quiescent leukemia cells J. Clin. Invest., 2017;127(6):2392-2406. 2017 [PMID: 28481221] (WB,, Human) WB Human
K Ishiguro, YL Zhu, ZP Lin, PG Penketh, K Shyam, R Zhu, RP Baumann, AC Sartorelli, TJ Rutherford, ES Ratner Cataloging antineoplastic agents according to their effectiveness against platinum-resistant and platinum-sensitive ovarian carcinoma cell lines J Transl Sci, 2016;2(2):117-124. 2016 [PMID: 27076919] (WB,, Human) WB Human
Yang G, Mercado-Uribe I, Multani A, Sen S, Shih I, Wong K, Gershenson D, Liu J RAS promotes tumorigenesis through genomic instability induced by imbalanced expression of Aurora-A and BRCA2 in midbody during cytokinesis. Int J Cancer, 2013;133(2):275-85. 2013 [PMID: 23319376] (WB,, Human) WB Human
Yang G, Chang B, Yang F Aurora kinase A promotes ovarian tumorigenesis through dysregulation of the cell cycle and suppression of BRCA2. Clin. Cancer Res., 2010;16(12):3171-81. 2010 [PMID: 20423983] (WB,, Human) WB Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BRCA2 Antibody (MAB2476) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for BRCA2 Antibody (MAB2476)

Discover related pathways, diseases and genes to BRCA2 Antibody (MAB2476). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for BRCA2 Antibody (MAB2476)

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Pathways for BRCA2 Antibody (MAB2476)

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PTMs for BRCA2 Antibody (MAB2476)

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Blogs on BRCA2.

FANCD2 and DNA damage repair
Fanconi anemia (FA) is a genetically inherited disorder that yields cytogenetic instability, hypersensitivity to DNA crosslinking compounds and defective DNA repair. A variety of genes have been identified within the FA pathway that are referred t...  Read full blog post.

Breast Cancer Infographic
Breast cancer is caused by malignant cells developing in breast tissue. It is the most commonly diagnosed cancer in women, but advancements in treatment options have seen the death rate decline since the 1990s. Common warning signs of breast cancer in...  Read full blog post.

RAD51: The cell's 'Mr. Fix-it'
RAD51 is a recombinase protein encoded by RAD51 gene in humans. Human RAD51 family members are highly similar to bacterial RecA and yeast Rad51, both biochemically and structurally. It is a 339-amino acid protein that plays an important role in homolo...  Read full blog post.

Breast Cancer and RAD51L1 Antibodies
In the United States, breast cancer is one of the most common cancers and the second leading cause of cancer related deaths in women. According to the American Cancer Society's most recent estimates for breast cancer in the United States, there are ab...  Read full blog post.

Determining DMC1's role in Homologus Recombination
The DMC1 gene encodes a 36.7 kDa nuclear protein involved in meiotic homologous recombination. This recombinase is functionally related to the yeast RAD51 and E. coli RecA genes. In contrast to RAD51, which functions in both mitotic and meiotic recomb...  Read full blog post.

Rad51 Antibody Reveals a Canine Model for Human Breast Cancer
Our antibody catalog includes an extensive range of Rad51 antibody reagents. Encoded by the RAD51 gene, the Rad51 protein plays a vital role in DNA repair, interacting with several other proteins, including BRCA1 and BRCA2, to effect homologous recomb...  Read full blog post.

Industrial Chemicals, Tumour Suppressor Genes and the Need for More Research
Human cancer research is the largest research area in our antibody database, with new oncogenes and cell lines being added all the time.Cancer triggers come from many sources, with a worrying amount of evidence to suggest that chemicals we’re in con...  Read full blog post.

Fanconi Antibodies and Cancer Research
We at Novus Biologicals have an extensive antibody databasedevoted to the 13 Fanconi anaemia complementation (FANC) genes, which are involved in the recognition and repair of damaged DNA.The core complex of 8 proteins (FANCA, B, C, E, F, G, L and M)...  Read full blog post.

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Bioinformatics

Gene Symbol BRCA2
Uniprot