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BRCA2 Antibody (234403) [Unconjugated]


Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.2 µg/mL of more
BRCA2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human BRCA2 Monoclonal Antibody (Catalog # MAB2476) at 5 µg/mL for 1 hour at room temperature more

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC

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BRCA2 Antibody (234403) [Unconjugated] Summary

E. coli-derived recombinant human BRCA2
Accession # P51587
Detects endogenous human BRCA2.
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 0.2 ug/mL
Read Publications using
MAB2476 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BRCA2 Antibody (234403) [Unconjugated]

  • BRCA1/BRCA2-containing complex, subunit 2
  • BRCA2
  • BRCC2
  • breast and ovarian cancer susceptibility gene, early onset
  • breast cancer 2 tumor suppressor
  • breast cancer 2, early onset
  • breast cancer susceptibility protein BRCA2
  • breast cancer type 2 susceptibility protein
  • FACD
  • FAD
  • FAD1
  • FANCD1Fanconi anemia, complementation group D1
  • Fanconi anemia group D1 protein
  • GLM3
  • PNCA2


BRCA2 is a nuclear protein that acts as a tumor suppressor. Germline mutation of BRCA2 accounts for many cases of familial breast and ovarian cancer. At the cellular level, BRCA2 is involved in homologous recombination (HR), a form of double-strand break repair. BRCA2 interacts with the Rad51 recombinase, another protein critical for HR, to preserve chromosomal integrity.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BRCA2 Antibody (MAB2476)(12)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-P, Western Blot.

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Flow Cytometry
Western Blot
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using MAB2476 Applications Species
Jichang Han, Meng Yu, Yiqin Bai, Jianzhong Yu, Fei Jin, Chen Li et al. Elevated CXorf67 Expression in PFA Ependymomas Suppresses DNA Repair and Sensitizes to PARP Inhibitors Cancer Cell 2020-12-01 [PMID: 33186520]
Y Ma, P Chen, JA Drisko, D Khabele, AK Godwin, Q Chen Pharmacological ascorbate induces 'BRCAness' and enhances the effects of Poly(ADP-Ribose) polymerase inhibitors against BRCA1/2 wild-type ovarian cancer Oncol Lett, 2020-01-31;19(4):2629-2638. 2020-01-31 [PMID: 32218813] (Western Blot, Human) Western Blot Human
Kim HS, Kim JW, Hwang IG et al. Expression of DNA Damage Response Markers in Early-Onset or Familial Gastric Cancers Asian Pac. J. Cancer Prev. 5/25/2019 [PMID: 31127894]
Hee Sung Kim, In Gyu Hwang, Hye Young Min, Yung‑Jue Bang, Woo Ho Kim Clinical significance of BRCA1 and BRCA2 mRNA and protein expression in patients with sporadic gastric cancer Oncology Letters 2019-03-08 [PMID: 30988810]
J Im, J Lawrence, D Seelig, RS Nho FoxM1-dependent RAD51 and BRCA2 signaling protects idiopathic pulmonary fibrosis fibroblasts from radiation-induced cell death Cell Death Dis, 2018-05-22;9(6):584. 2018-05-22 [PMID: 29789556] (Western Blot, Human) Western Blot Human
Silvia Maifrede, Margaret Nieborowska-Skorska, Katherine Sullivan-Reed, Yashodhara Dasgupta, Paulina Podszywalow-Bartnicka, Bac Viet Le et al. Tyrosine kinase inhibitor–induced defects in DNA repair sensitize FLT3(ITD)-positive leukemia cells to PARP1 inhibitors Blood 2018-07-05 [PMID: 29784639]
S Maifrede, K Martin, P Podszywalo, K Sullivan, SK Langer, R Nejadi, Y Dasgupta, M Hulse, D Gritsyuk, M Nieborowsk, LN Lupey-Gree, H Zhao, K Piwocka, MA Wasik, I Tempera, T Skorski IGH/MYC Translocation Associates with BRCA2 Deficiency and Synthetic Lethality to PARP1 Inhibitors Mol. Cancer Res., 2017-06-20;0(0):. 2017-06-20 [PMID: 28634224] (Flow Cytometry, Western Blot, Human) Flow Cytometry, Western Blot Human
M Nieborowsk, K Sullivan, Y Dasgupta, P Podszywalo, G Hoser, S Maifrede, E Martinez, D Di Marcant, E Bolton-Gil, K Cramer-Mor, J Lee, M Li, A Slupianek, D Gritsyuk, S Cerny-Reit, I Seferynska, T Stoklosa, L Bullinger, H Zhao, V Gorbunova, K Piwocka, P Valent, CI Civin, M Muschen, JE Dick, JC Wang, S Bhatia, R Bhatia, K Eppert, MD Minden, SM Sykes, T Skorski Gene expression and mutation-guided synthetic lethality eradicates proliferating and quiescent leukemia cells J. Clin. Invest., 2017;127(6):2392-2406. 2017 [PMID: 28481221] (Western Blot, Human) Western Blot Human
K Ishiguro, YL Zhu, ZP Lin, PG Penketh, K Shyam, R Zhu, RP Baumann, AC Sartorelli, TJ Rutherford, ES Ratner Cataloging antineoplastic agents according to their effectiveness against platinum-resistant and platinum-sensitive ovarian carcinoma cell lines J Transl Sci, 2016-03-05;2(2):117-124. 2016-03-05 [PMID: 27076919] (Western Blot, Human) Western Blot Human
Yang G, Mercado-Uribe I, Multani A, Sen S, Shih I, Wong K, Gershenson D, Liu J RAS promotes tumorigenesis through genomic instability induced by imbalanced expression of Aurora-A and BRCA2 in midbody during cytokinesis. Int J Cancer, 2013-02-12;133(2):275-85. 2013-02-12 [PMID: 23319376] (IHC, Western Blot, Human) IHC, Western Blot Human
Show All 12 Publications.

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Product General Protocols

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Secondary Antibodies


Isotype Controls

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Blogs on BRCA2.

FANCD2 and DNA damage repair
Fanconi anemia (FA) is a genetically inherited disorder that yields cytogenetic instability, hypersensitivity to DNA crosslinking compounds and defective DNA repair. A variety of genes have been identified within the FA pathway that are referred t...  Read full blog post.

Breast Cancer Infographic
Breast cancer is caused by malignant cells developing in breast tissue. It is the most commonly diagnosed cancer in women, but advancements in treatment options have seen the death rate decline since the 1990s. Common warning signs of breast cancer in...  Read full blog post.

RAD51: The cell's 'Mr. Fix-it'
RAD51 is a recombinase protein encoded by RAD51 gene in humans. Human RAD51 family members are highly similar to bacterial RecA and yeast Rad51, both biochemically and structurally. It is a 339-amino acid protein that plays an important role in homolo...  Read full blog post.

Breast Cancer and RAD51L1 Antibodies
In the United States, breast cancer is one of the most common cancers and the second leading cause of cancer related deaths in women. According to the American Cancer Society's most recent estimates for breast cancer in the United States, there are ab...  Read full blog post.

Determining DMC1's role in Homologus Recombination
The DMC1 gene encodes a 36.7 kDa nuclear protein involved in meiotic homologous recombination. This recombinase is functionally related to the yeast RAD51 and E. coli RecA genes. In contrast to RAD51, which functions in both mitotic and meiotic recomb...  Read full blog post.

Rad51 Antibody Reveals a Canine Model for Human Breast Cancer
Our antibody catalog includes an extensive range of Rad51 antibody reagents. Encoded by the RAD51 gene, the Rad51 protein plays a vital role in DNA repair, interacting with several other proteins, including BRCA1 and BRCA2, to effect homologous recomb...  Read full blog post.

Industrial Chemicals, Tumour Suppressor Genes and the Need for More Research
Human cancer research is the largest research area in our antibody database, with new oncogenes and cell lines being added all the time.Cancer triggers come from many sources, with a worrying amount of evidence to suggest that chemicals we’re in con...  Read full blog post.

Fanconi Antibodies and Cancer Research
We at Novus Biologicals have an extensive antibody databasedevoted to the 13 Fanconi anaemia complementation (FANC) genes, which are involved in the recognition and repair of damaged DNA.The core complex of 8 proteins (FANCA, B, C, E, F, G, L and M)...  Read full blog post.

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Gene Symbol BRCA2