Rad50 Antibody (13B3)

Images

 
Western Blot: Rad50 Antibody (13B3) [NB100-147] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Rad50 Antibody (13B3) [NB100-147] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad50 protein stained by Rad50 antibody [13B3] diluted at 1:200. Red: ...read more
Immunohistochemistry-Paraffin: Rad50 Antibody (13B3) [NB100-147] - Human lung cancer. Rad50 stained by Rad50 antibody [13B3] diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Rad50 Antibody (13B3) [NB100-147] - Analysis of RAD50 in 293T cell lysate using anti-RAD50 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Rad50 Antibody (13B3) [NB100-147] - CAL 27 xenograft. Rad50 antibody [13B3] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Rad50 Antibody (13B3) [NB100-147] - PC-3 xenograft. Rad50 antibody [13B3] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Rad50 Antibody (13B3) [NB100-147] - Human lung. Rad50 antibody [13B3] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Rad50 Antibody (13B3) [NB100-147] - Human lung cancer. Rad50 stained by Rad50 antibody [13B3] diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Genetic Strategies: Knockdown Validated: Rad50 Antibody (13B3) [NB100-147] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, In vitro, IP, KD
Clone
13B3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Rad50 Antibody (13B3) Summary

Immunogen
Amino acids 1-425 of Rad50 expressed in E. coli.
Localization
Nuclear
Specificity
This is specific for Rad50.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RAD50
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation
  • In vitro assay
  • Knockdown Validated
Application Notes
IHC usage reported in scientific literature (PMID: 16431910). in vitro Assay dependent, IP Assay dependent.
Theoretical MW
153 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Rad50 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-147 in the following applications:

Publications
Read Publications using
NB100-147 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for Rad50 Antibody (13B3)

  • DNA repair protein RAD50
  • EC 3.6
  • EC 3.6.1.15
  • EC 3.6.3
  • EC 3.6.3.27
  • hRad50
  • NBSLD
  • RAD50 (S. cerevisiae) homolog
  • RAD50 homolog (S. cerevisiae)
  • Rad50
  • RAD502
  • RAD50-2

Background

Human Rad50, a 153kDa protein involved in DNA double strand break repair, is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-147
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-P, In vitro, IP, KD

Publications for Rad50 Antibody (NB100-147)(76)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
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(2)
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Human
(68)
Mouse
(3)
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Showing Publications 1 - 10 of 76. Show All 76 Publications.
Publications using NB100-147 Applications Species
Sivalingam VN, Latif A, Kitson S et al. Hypoxia and hyperglycaemia determine why some endometrial tumours fail to respond to metformin Br. J. Cancer Dec 10 2019 [PMID: 31819173] (IHC-P, Human) IHC-P Human
Marie-Egyptienne DT, Chaudary N, Kalliomaki T et al. Cancer initiating-cells are enriched in the CA9 positive fraction of primary cervix cancer xenografts. Oncotarget. Jan 3 2017 [PMID: 27901496] (IHC-P, Mouse) IHC-P Mouse
Semba H, Takeda N, Isagawa T et al. HIF-1alpha-PDk1 axis-induced active glycolysis plays an essential role in macrophage migratory capacity. Nat Commun. May 18 2016 [PMID: 27189088] (IHC, Mouse) IHC Mouse
Samba-Louaka A, Pereira Jm, Nahori Ma et al. Listeria monocytogenes Dampens the DnA Damage Response. PLoS Pathog. 2014 Oct 01 [PMID: 25340842] (ICC/IF, Human) ICC/IF Human
Khongkow P, Karunarathna U, Khongkow M et al. FOXM1 targets NBS1 to regulate DNA damage-induced senescence and epirubicin resistance. Oncogene. 2013 Oct 21 [PMID: 24141789] (WB, Mouse, Human) WB Mouse, Human
Masuda Y, Noguchi K, Segawa H et al. Novel regulatory role for kaposi's sarcoma-associated herpesvirus-encoded vFLIP in chemosensitization to bleomycin. Biochem Biophys Res Commun 2011 Nov [PMID: 22037577] (Human) Human
Cheng Q, Barboule N, Frit P et al. ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks. Nucleic Acids Res 2011 Dec [PMID: 21880593] (Human) Human
Soares D, Battistella A, Rocca C et al. Ataxia telangiectasia mutated- and Rad3-related kinase drives both the early and the late DNA-damage response to the monofunctional antitumour alkylator S23906 Biochem J 2011 Jul 1 [PMID: 21470188] (WB, Human) WB Human
Baydoun HH, Pancewicz J, Nicot C. Human T-lymphotropic type 1 virus p30 inhibits homologous recombination and favors unfaithful DNA repair. Blood 2011 Jun [PMID: 21427292] (Human) Human
Stoepker C, Hain K, Schuster B et al. SLX4, a coordinator of structure-specific endonucleases, is mutated in a new Fanconi anemia subtype. Nat Genet 2011 Feb [PMID: 21240277] (Human) Human
Show All 76 Publications.

Review for Rad50 Antibody (NB100-147) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-147:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Rad50 NB100-147
Enlarge
reviewed by:
Anatoli Grechov
WB Human 12/17/2014
View

Summary

ApplicationWestern Blot
Sample Tested293T lysate
SpeciesHuman

Blocking

Blocking Details5% BSA in PBS

Primary Anitbody

Dilution Ratio1:2000 diluted in PBS with triton X-100, blot was incubated at room temperature on a rocker

Secondary Antibody

Secondary Descriptionanti-mouse IgG HRP conjugate (Jackson Labs)
Secondary Manufacturer Cat#115-005-174
Secondary Concentration1:15000

Details

Detection NotesProbed using Visualizer Western Blot Detection Kit (Millipore) and captured on X-ray films, 30 sec exposure

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rad50 Antibody (NB100-147) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for Rad50 Antibody (NB100-147)

Discover related pathways, diseases and genes to Rad50 Antibody (NB100-147). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Rad50.

RAD50 and DNA Damage Response
DNA repair protein RAD50 is a component of the MRN complex (Mre11-RAD50-Nbs1) responsible for DNA double strand break (DSB) repair. DSBs are caused by ionizing radiation, certain chemotherapy drugs, metabolic reactive oxygen species (ROS), replication...  Read full blog post.

The MRE11 Complex and DNA Damage Response
The maintenance of genome stability depends on the DNA damage response (DDR) which is a complex signaling network including cell cycle checkpoints, DNA repair and damage tolerance pathways. The DDR complex has the ability to sense DNA damage and tra...  Read full blog post.

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Recent Reviews

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5
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4
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1
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Anatoli Grechov
12/17/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol RAD50
Entrez