Chk1 Antibody

Images

 
Western Blot: Chk1 Antibody [NB100-464] - Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TMCK cells. Antibodies: Affinity purified rabbit anti-Chk1 ...read more
Immunocytochemistry/ Immunofluorescence: Chk1 Antibody [NB100-464] - NBF-fixed HeLa cells treated with neocarzinostatin. Antibody used at a dilution of 1:1,000 (1ug/ml).
Immunohistochemistry: Chk1 Antibody [NB100-464] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: Chk1 Antibody [NB100-464] - Whole cell lysate (20 ug) from HeLa cells. Antibody used at the indicated concentrations.
Immunohistochemistry-Paraffin: Chk1 Antibody [NB100-464] - Human testicular seminoma. Antibody used at a dilution of 1:1000 (1ug/ml).
Immunohistochemistry: Chk1 Antibody [NB100-464] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Proximity Ligation Assay: Chk1 Antibody [NB100-464] - Secondary-conjugate Duolink in Hela cells. goat anti-human CHK1 (NB100-274) and rabbit anti-human CHK1 (NB100-464). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: Chk1 Antibody [NB100-464] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human HdmX (NBP2-22228) and rabbit anti-human CHK1 (NB100-464). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: Chk1 Antibody [NB100-464] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RAD17 (NB100-272) and rabbit anti-human CHK1 (NB100-464). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: Chk1 Antibody [NB100-464] - Secondary-conjugate Duolink In Situ PLA in Hela cells. goat anti-human USP7 (NBP2-22295) and rabbit anti-human CHK1 (NB100-464). Image merged from DAPI (2ms), ...read more
Proximity Ligation Assay: Chk1 Antibody [NB100-464] - Secondary-conjugate Duolink In Situ PLA in Hela cells. goat anti-human RelA (NBP2-22297) and rabbit anti-human CHK1 (NB100-464). Image merged from DAPI (2ms), ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

Chk1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 250 and 300 of human Checkpoint Kinase 1 using the numbering given in SwissProt entry O14757 (GeneID 1111).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHEK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:25000
  • Immunocytochemistry/Immunofluorescence 1:500-1:2000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Proximity Ligation Assay 1:1000-1:10000
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Chk1 Lysate (NBL1-09146)
Publications
Read Publications using
NB100-464 in the following applications:

  • IHC
    1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-305, NB100-322, NB100-97831

Alternate Names for Chk1 Antibody

  • Checkpoint, S. pombe, homolog of, 1
  • CHEK1
  • CHK1 (checkpoint, S.pombe) homolog
  • CHK1 checkpoint homolog (S. pombe)
  • Chk1
  • CHK1serine/threonine-protein kinase Chk1
  • EC 2.7.11
  • EC 2.7.11.1

Background

In response to DNA damage and replication blocks, cells prevent cell cycle progression through the control of critical cell cycle regulators. Chk1 is an essential kinase for embryonic development and regulating mitotic progression in response to DNA damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Chk1 Antibody (NB100-464)(5)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Chk1 Antibody (NB100-464) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CHEK1
Entrez
Uniprot