Chk1 Antibody

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Biological Strategies: Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells. Western blot of ORC2 in HBEC and 293T cell lines. Ponceau S staining of total protein or immunoblot of ...read more
Immunohistochemistry-Paraffin: Chk1 Antibody [NB100-464] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Biological Strategies: Western Blot: Chk1 Antibody [NB100-464] - Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TMCK cells. Antibodies: Affinity ...read more
Immunoprecipitation: Chk1 Antibody [NB100-464] - Detection of human Chk1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Chk1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 250 and 300 of human Checkpoint Kinase 1 using the numbering given in SwissProt entry O14757 (GeneID 1111).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHEK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:10000-1:25000
Application Notes
Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Chk1 Overexpression Lysate
Publications
Read Publications using
NB100-464 in the following applications:

  • IHC
    2 publications
  • IP
    1 publication
  • WB
    6 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29287241).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-305, NB100-322, NB100-97831

Alternate Names for Chk1 Antibody

  • Checkpoint, S. pombe, homolog of, 1
  • CHEK1
  • CHK1 (checkpoint, S.pombe) homolog
  • CHK1 checkpoint homolog (S. pombe)
  • Chk1
  • CHK1serine/threonine-protein kinase Chk1
  • EC 2.7.11
  • EC 2.7.11.1

Background

Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee 1 in vitro, providing evidence that the hyperphosphorylated form of Wee 1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1. Chk1 is phosphorylated on Serine 345 (S345) in response to UV, IR and hydroxyurea (HU). Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development and tumor suppression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Ca, Hu, Ma, Mu, Rt
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Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
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Applications: ICC/IF, IHC, IHC-P, WB
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Species: Pm, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
AF2288
Species: Hu
Applications: ICC, IHC, Simple Western, WB
NBP2-33466
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
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Species: Hu
Applications: IHC, IHC-P, IP, WB
MAB1648
Species: Hu, Mu, Rt
Applications: IHC, WB
NBP2-00776
Species: Ca, Hu, Pm, Mu, Rt
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NBP2-46085
Species: Hu
Applications: IHC, WB
H00027065-M01
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Applications: ELISA, ICC/IF, S-ELISA, WB
AF6000
Species: Hu, Mu
Applications: IHC, Simple Western, WB
NB100-598
Species: Hu
Applications: CyTOF-ready, Flow, ICC/IF, IP, WB
NB100-143
Species: Ha, Hu, Ma, Mu, Pm
Applications: ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP, ISH, KO, PAGE, WB
NBP2-24457
Species: Hu, Mu, Pm
Applications: IHC, IHC-P, WB

Publications for Chk1 Antibody (NB100-464)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IP, WB.


Filter By Application
IHC
(2)
IP
(1)
WB
(6)
All Applications
Filter By Species
Human
(8)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-464 Applications Species
Lee KY, Dutta A Chk1 promotes non-homologous end joining in G1 through direct phosphorylation of ASF1A Cell reports Jan 26 2021 [PMID: 33503415] (IP, Human) IP Human
Morris BB, Wages NA, Grant PA, et al. MYBL2-Driven Transcriptional Programs Link Replication Stress and Error-prone DNA Repair With Genomic Instability in Lung Adenocarcinoma Frontiers in oncology Jan 8 2020 [PMID: 33489883] (WB, Human) WB Human
Shibata E, Dutta A A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteins The Journal of biological chemistry Dec 11 2020 [PMID: 32989049] (WB, Human) WB Human
Bai X, Wang J, Huo L et al. Serine/Threonine Kinase CHEK1-Dependent Transcriptional Regulation of RAD54L Promotes Proliferation and Radio Resistance in Glioblastoma. Transl Oncol 2017 Dec 26 [PMID: 29287241] (Mouse) Mouse
Shibata E, Kiran M, Shibata Y et al. Two subunits of human ORC are dispensable for DNA replication and proliferation Elife. 2016 Nov 30 [PMID: 27906128] (WB, Human) WB Human
Yun H, Shi R, Yang Q et al. Over expression of hRad9 protein correlates with reduced chemosensitivity in breast cancer with administration of neoadjuvant chemotherapy Sci Rep. 2014 Dec 17 [PMID: 25520248] (IHC, Human) IHC Human
Terai K, Shibata E, Abbas T, Dutta A. Degradation of p12 Subunit by CRL4Cdt2 E3 Ligase Inhibits Fork Progression after DNA Damage. J Biol Chem. 2013 Oct 18 [PMID: 24022480] (WB, Human) WB Human
Jazaeri AA, Shibata E, Park J et al. Overcoming platinum resistance in preclinical models of ovarian cancer using the neddylation inhibitor MLN4924. Mol Cancer Ther. 2013 Aug 12 [PMID: 23939375] (WB, Human) WB Human
Hong J, Hu K, Yuan Y et al. CHK1 targets spleen tyrosine kinase (L) for proteolysis in hepatocellular carcinoma J Clin Invest 2012 Jun 1 [PMID: 22585575] (IHC, WB, Human) IHC, WB Human
Karnani N, Dutta A. The effect of the intra-S-phase checkpoint on origins of replication in human cells. Genes Dev;25(6):621-33. 2011 Mar 15. [PMID: 21406556]
Show All 11 Publications.

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Bioinformatics

Gene Symbol CHEK1
Entrez
Uniprot