Biological Strategies: Western Blot: Chk1 Antibody [NB100-464] - Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TMCK cells. Antibodies: Affinity ...read more
Immunohistochemistry: Chk1 Antibody [NB100-464] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry 1:1,000 - 1:5,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
54 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Mouse reactivity reported in scientific literature (PMID: 29287241).
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.09% Sodium Azide
Immunogen affinity purified
This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-305, NB100-322, NB100-97831
Alternate Names for Chk1 Antibody
Checkpoint, S. pombe, homolog of, 1
CHK1 (checkpoint, S.pombe) homolog
CHK1 checkpoint homolog (S. pombe)
CHK1serine/threonine-protein kinase Chk1
In response to DNA damage and replication blocks, cells prevent cell cycle progression through the control of critical cell cycle regulators. Chk1 is an essential kinase for embryonic development and regulating mitotic progression in response to DNA damage.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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