ATR Antibody (2B5)


Western Blot: ATR Antibody (2B5) [NB100-308] - Human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: ATR Antibody (2B5) [NB100-308] - Analysis of HeLa, using antibody at 1:100 dilution.
Western Blot: ATR Antibody (2B5) [NB100-308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] diluted at more
Orthogonal Strategies: Western Blot: ATR Antibody (2B5) [NB100-308] - Raji whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody diluted at 1:500.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Func, ICC/IF, IP, KO
Validated by:

Orthogonal Strategies


Order Details

ATR Antibody (2B5) Summary

Recombinant fragment, corresponding to amino acids 710-1100 of Human ATR.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Functional
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • In vitro assay
  • Knockout Validated
  • Western Blot 1:500-1:3000
Application Notes
Functional and IP usages reported in (PMID:18202016). Knockout validation (PMID: 31757956). Use In vitro assay reported in scientific literature (PMID: 18202016). IP, Functional, ICC/IF- Assay dependent.
Theoretical MW
317 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Raji Whole Cell Lysate
Read Publications using
NB100-308 in the following applications:

  • 14 publications
  • IP
    14 publications
  • KO
    1 publication
  • WB
    4 publications

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No Preservative
Protein G purified

Alternate Names for ATR Antibody (2B5)

  • ataxia telangiectasia and Rad3 related
  • Ataxia telangiectasia and Rad3-related protein
  • ATR
  • EC
  • FRAP-related protein 1
  • FRP1
  • FRP1FRAP-related protein-1
  • MEC1
  • protein kinase ATR
  • Rad3 related protein
  • SCKL1
  • SCKLMEC1, mitosis entry checkpoint 1, homolog
  • serine/threonine-protein kinase ATR


Ataxia Telangiectasia Mutated (ATM) and Rad3- related (ATR) protein is a member of the phosphatidyl-inositol 3-kinase (PI 3-kinase) like family of protein kinases. ATR has a predicted molecular mass of approximately 301 kDa, and it has been shown to be involved in cellular responses to DNA damage. Specifically, it plays a role in checkpoint activation in response to either the presence of stalled replication forks, or by the presence of lesions caused by UV light. Inactivation of ATR is an embryonic lethal event in mice.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATR Antibody (NB100-308)(21)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Func, IP, KO, WB.

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Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NB100-308 Applications Species
Uchida C, Niida H, Sakai S et al. p130RB2 positively contributes to ATR activation in response to replication stress via the RPA32-ETAA1 axis Biochimica et biophysica acta. Molecular cell research 2023-05-17 [PMID: 37201767]
Zeng F, Parker K, Zhan Y et al. Upregulated DNA Damage-Linked Biomarkers in Parkinson's Disease Model Mice ASN Neuro 2023-01-23 [PMID: 36683340] (WB) WB
Chakraborty P, Hiom K DHX9-dependent recruitment of BRCA1 to RNA promotes DNA end resection in homologous recombination Nature communications 2021-07-05 [PMID: 34226554] (WB) WB
Kim W, Zhao F, Wu R et al. ZFP161 regulates replication fork stability and maintenance of genomic stability by recruiting the ATR/ATRIP complex Nat Commun 2019-11-22 [PMID: 31757956] (KO, WB, Mouse) KO, WB Mouse
Haro KJ, Sheth A, Scheinberg DA et al. Dysregulation of IRP1-Mediated Iron Metabolism Causes Gamma Ray-specific Radioresistance in Leukemia Cells. PLoS One 2012-01-01 [PMID: 23155415] (WB, Human) WB Human
Oku T, Nakano M, Kaneko Y et al. Constitutive turnover of phosphorylation at Thr-412 of human p57/coronin-1 regulates the interaction with actin. J Biol Chem 2012-12-14 [PMID: 23100250] (Human) Human
Ha K, Fiskus W, Rao R et al. Hsp90 inhibitor-mediated disruption of chaperone association of ATR with hsp90 sensitizes cancer cells to DNA damage. Mol Cancer Ther 2011-07-01 [PMID: 21566061] (IP, Func, Human) IP, Func Human
Chen Y, Chen CF, Riley DJ et al. Nek1 kinase functions in DNA damage response and checkpoint control through a pathway independent of ATM and ATR. Cell Cycle 2011-02-01 [PMID: 21301226] (Func, IP, Human) Func, IP Human
Zuco V, Benedetti V, Zunino F. ATM- and ATR-mediated response to DNA damage induced by a novel camptothecin, ST1968. Cancer Lett 2010-06-01 [PMID: 20042274] (IP, Func, Human) IP, Func Human
Cui B, Johnson SP, Bullock N et al. Bifunctional DNA alkylator 1,3-bis(2-chloroethyl)-1-nitrosourea activates the ATR-Chk1 pathway independently of the mismatch repair pathway. Mol Pharmacol 2009-06-01 [PMID: 19261750] (Func, IP, Human) Func, IP Human
Show All 21 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATR Antibody (NB100-308) (0)

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Control Lysate(s)

Secondary Antibodies


Isotype Controls

Additional ATR Products

Array NB100-308

Research Areas for ATR Antibody (NB100-308)

Find related products by research area.

Blogs on ATR.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

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Gene Symbol ATR