ATR Antibody (2B5)

Images

 
Western Blot: ATR Antibody (2B5) [NB100-308] - Human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: ATR Antibody (2B5) [NB100-308] - Analysis of HeLa, using antibody at 1:100 dilution.
Western Blot: ATR Antibody (2B5) [NB100-308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] diluted at ...read more
Western Blot: ATR Antibody (2B5) [NB100-308] - Raji whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody diluted at 1:500.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Func, ICC/IF, IP
Clone
2B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

ATR Antibody (2B5) Summary

Immunogen
Recombinant fragment, corresponding to amino acids 710-1100 of Human ATR.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
ATR
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Functional
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
Application Notes
Functional and IP usages reported in scientific literature (Kulkarni, A. et al). Functional Assay usage reported in scientific literature. ICC/IF usage reported in scientifc literature.
Theoretical MW
317 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Raji Lysate (NB820-59466)
Publications
Read Publications using
NB100-308 in the following applications:

  • 14 publications
  • IP
    14 publications
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for ATR Antibody (2B5)

  • ataxia telangiectasia and Rad3 related
  • Ataxia telangiectasia and Rad3-related protein
  • ATR
  • EC 2.7.11.1
  • FRAP-related protein 1
  • FRP1
  • FRP1FRAP-related protein-1
  • MEC1
  • protein kinase ATR
  • Rad3 related protein
  • SCKL1
  • SCKLMEC1, mitosis entry checkpoint 1, homolog
  • serine/threonine-protein kinase ATR

Background

Ataxia Telangiectasia Mutated (ATM) and Rad3- related (ATR) protein is a member of the phosphatidyl-inositol 3-kinase (PI 3-kinase) like family of protein kinases. ATR has a predicted molecular mass of approximately 301 kDa, and it has been shown to be involved in cellular responses to DNA damage. Specifically, it plays a role in checkpoint activation in response to either the presence of stalled replication forks, or by the presence of lesions caused by UV light. Inactivation of ATR is an embryonic lethal event in mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATR Antibody (NB100-308)(17)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Func, IP, WB.


Filter By Application
Func
(14)
IP
(14)
WB
(1)
All Applications
Filter By Species
Human
(16)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB100-308 Applications Species
Haro KJ, Sheth A, Scheinberg DA. Dysregulation of IRP1-Mediated Iron Metabolism Causes Gamma Ray-specific Radioresistance in Leukemia Cells PLoS One 2012 [PMID: 23155415] (WB, Human) WB Human
Oku T, Nakano M, Kaneko Y et al. Constitutive turnover of phosphorylation at Thr-412 of human p57/coronin-1 regulates the interaction with actin. J Biol Chem 2012 Dec 14 [PMID: 23100250] (Human) Human
Ha K, Fiskus W, Rao R et al. Hsp90 inhibitor-mediated disruption of chaperone association of ATR with hsp90 sensitizes cancer cells to DNA damage. Mol Cancer Ther 2011 Jul [PMID: 21566061] (Func, IP, Human) Func, IP Human
Chen Y, Chen CF, Riley DJ et al. Nek1 kinase functions in DNA damage response and checkpoint control through a pathway independent of ATM and ATR. Cell Cycle 2011 Feb [PMID: 21301226] (Func, IP, Human) Func, IP Human
Zuco V, Benedetti V, Zunino F. ATM- and ATR-mediated response to DNA damage induced by a novel camptothecin, ST1968. Cancer Lett 2010 Jun [PMID: 20042274] (Func, IP, Human) Func, IP Human
Cui B, Johnson SP, Bullock N et al. Bifunctional DNA alkylator 1,3-bis(2-chloroethyl)-1-nitrosourea activates the ATR-Chk1 pathway independently of the mismatch repair pathway. Mol Pharmacol 2009 Jun [PMID: 19261750] (Func, IP, Human) Func, IP Human
Katsuki Y, Nakada S, Yokoyama T et al. Caffeine yields aneuploidy through asymmetrical cell division caused by misalignment of chromosomes. Cancer Sci 2008 Aug [PMID: 18754864] (Func, IP, Human) Func, IP Human
Kulkarni A, Das KC. Differential roles of ATR and ATM in p53, Chk1, and histone H2AX phosphorylation in response to hyperoxia: ATR-dependent ATM activation. Am J Physiol Lung Cell Mol Physiol 2008 May [PMID: 18344416] (Func, IP, Human) Func, IP Human
Liu X, Matsuda A, Plunkett W. Ataxia-telangiectasia and Rad3-related and DNA-dependent protein kinase cooperate in G2 checkpoint activation by the DNA strand-breaking nucleoside analogue 2'-C-cyano-2'-deoxy-1-beta-D-arabino-pentofuranosylcytosine. Mol Cancer Ther 2008 Jan [PMID: 18202016] (Func, IP, Human) Func, IP Human
Manthey KC, Opiyo S, Glanzer JG et al. NBS1 mediates ATR-dependent RPA hyperphosphorylation following replication-fork stall and collapse. J Cell Sci 2007 Dec [PMID: 18003706] (Func, IP, Human) Func, IP Human
Show All 17 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATR Antibody (NB100-308) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-308AF405
Alexa Fluor 488 Labeled NB100-308AF488
Alexa Fluor 647 Labeled NB100-308AF647
Alexa Fluor 700 Labeled NB100-308AF700
DyLight 350 Labeled NB100-308UV
DyLight 405 Labeled NB100-308V
DyLight 488 Labeled NB100-308G
DyLight 680 Labeled NB100-308FR
DyLight 755 Labeled NB100-308IR

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Bioinformatics Tool for ATR Antibody (NB100-308)

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Research Areas for ATR Antibody (NB100-308)

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Blogs on ATR.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

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Bioinformatics

Gene Symbol ATR
Entrez
Uniprot