MyD88 Antibody

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Western Blot: MyD88 Antibody [NB100-56698] - Analysis of MyD88 in human spleen cell lysate using 0.5 ug/ml of NB100-56698.
Immunocytochemistry/ Immunofluorescence: MyD88 Antibody [NB100-56698] - MyD88 antibody was tested in Daudi cells at a 1:20 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin ...read more
Immunohistochemistry-Paraffin: MyD88 Antibody [NB100-56698] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human liver using MyD88 Antibody at 1:100 dilution. This antibody generated a very ...read more
Immunohistochemistry-Paraffin: MyD88 Antibody [NB100-56698] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human liver using MyD88 Antibody at 1:100 dilution. This antibody generated a very ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Eq, Fi, GP, Pm, Sh, Xp, ZeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

MyD88 Antibody Summary

Immunogen
A synthetic peptide corresponding to human MyD88 (between amino acids 200-270).
Localization
Cytoplasm
Specificity
Because of high sequence homology, this antibody is expected to detect multiple isoforms of MyD88 protein.
Predicted Species
Porcine (100%), Equine (100%), Guinea Pig (94%), Xenopus (93%), Primate (100%), Fish (93%), Bovine (100%), Zebrafish (93%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYD88
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 2-5 ug/ml
  • Immunohistochemistry-Paraffin 2-5 ug/ml
Antagonist
Publications
Read Publications using
NB100-56698 in the following applications:

Reactivity Notes

The immunogen sequence shows over 90% homology with several vertebrates and this antibody is expected to cross-react with multiple species among vertebrate animals.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for MyD88 Antibody

  • MyD88
  • MYD88D
  • myeloid differentiation primary response gene (88)
  • myeloid differentiation primary response protein MyD88

Background

MyD88, a protein involved in interleukin-1 (IL-1) mediated signalling was originally isolated as myeloid differentiation primary response gene (3,4). MyD88 possesses a N-terminal death domain similar to cytoplasmic segments of TNF receptor 1, Fas, and C-terminal region related to IL-1 and Toll receptors. Overexpression of MyD88 induces activation the c-Jun N-terminal kinase and NF-kB through its death domain (1,2). The C-terminus of MyD88 interacts with the IL-1 receptor and blocks NF-kB activation induced by IL-1, but not by NF-kB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-56698
Species: Hu, Mu, Rt, Po, Bv, Eq, Fi, GP, Pm, Sh, Xp, Ze
Applications: WB, ICC/IF, IHC, IHC-P
Supplier Logo

Publications for MyD88 Antibody (NB100-56698)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(7)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
Rat
(4)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-56698 Applications Species
Popiolek-Barczyk K, Piotrowska A, Makuch W, Mika J. Biphalin, a Dimeric Enkephalin, Alleviates LPS-Induced Activation in Rat Primary Microglial Cultures in Opioid Receptor-Dependent and Receptor-Independent Manners.. Neural Plast. Jun 2 2017 [PMID: 28573049] (WB, Rat) WB Rat
Shi M, Zhang Y, Liu L et al. MAP1S regulates the phagocytosis of bacteria and TLR signaling. J. Biol. Chem. 2015 Nov 12 [PMID: 26565030] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Dumas A, Amiable N, de Rivero Vaccari JP et al. The Inflammasome Pyrin Contributes to Pertussis Toxin-Induced IL-1B Synthesis, Neutrophil Intravascular Crawling and Autoimmune Encephalomyelitis. PLoS Pathog. 2014 May 01 [PMID: 24875775] (WB, Mouse)

Details:
Fig 4c, d: peritoneal leukocytes
WB Mouse
Li Y, Zhang H, Zhang H et al. Toll-like receptor 4 signaling contributes to paclitaxel-induced peripheral neuropathy. J Pain 2014 Apr 19 [PMID: 24755282] (Rat)

Details:
Spinal cord: WB (Figs 1C, 2A, 8A), MyD88 was detected at ~31kDa
Rat
Harvey SA, Dangi A, Tandon A et al. The Transcriptomic Response of Rat Hepatic Stellate Cells to Endotoxin: Implications for Hepatic Inflammation and Immune Regulation. PLoS One 2013 Dec 9 [PMID: 24349206] (WB, Rat) WB Rat
Pathak SK, Basu S, Basu KK et al. Direct extracellular interaction between the early secreted antigen ESAT-6 of Mycobacterium tuberculosis and TLR2 inhibits TLR signaling in macrophages. Nat Immunol. 2007 Jun [PMID: 17486091] (WB)

Details:
Products cited (RAW cells): 1. IMG-178 (MyD88): WB, Figs. 3a, 5e,f. 2. IMG-441 (IRAK-4): WB, Figs. 3a, 5e,f. 3. IMK-501 (Phospho-IkBa ActivELISA Kit): Figs. 2g, 6c.
WB
Palladino MA, Johnson TA, Gupta R et al. Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract. Biol Reprod. 2007 Jun [PMID: 17314314] (WB, Rat)

Details:
Anitbodies cited: The following antibodies were used for WB in Fig 2 in rat testis, epididymis, and vas deferens: TLR1 (IMG-5012), TLR2 (IMG-545), TLR5 (IMG-580), TLR6 (IMG-527), TLR7 (IMG-581), TLR9 (IMG-305A), and TLR12 (IMG-5034). MYD88 (IMG-178) and T
WB Rat
Saccani A, Schioppa T, Porta C et al. p50 nuclear factor-kappaB overexpression in tumor-associated macrophages inhibits M1 inflammatory responses and antitumor resistance. Cancer Res. 2006 Dec 1 [PMID: 17145890] (WB, Human)

Details:
WB (human peritoneal exudate cells and tumor-associated macrophages), Fig. 1C.
WB Human
Macagno A, Molteni M, Rinaldi A et al. A cyanobacterial LPS antagonist prevents endotoxin shock and blocks sustained TLR4 stimulation required for cytokine expression. J Exp Med. 2006 Jun 12 [PMID: 16717116]
Srivastava MD, Srivastava BI. Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line. Leuk Res. 2005 Jul [PMID: 15927677]
Show All 11 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Blogs on MyD88.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

MYD88 Expression and Tumorigenesis
MyD88, also called myeloid differentiation primary response gene 88, encodes a cytosolic adapter protein that plays an essential role in innate and adaptive immune responses. The innate immune system recognizes the presence of bacterial pathogens thro...  Read full blog post.

TLR7 and Immune Response Regulation
Toll-like receptor 7 (TLR7) is a protein encoded by the TLR7 gene in humans and is a member of TLR family. TLRs controls host immune response against pathogens (e.g. viruses, bacteria and fungi) through recognition of pathogen-associated molecular pat...  Read full blog post.

MYD88: Fanning Inflammation and Immune Responses
Myeloid differentiation primary response gene 88 (MYD88) encodes a cytosolic adapter protein that plays an essential role in innate and adaptive immune responses. MYD88 protein functions as an essential signal transducer in the interleukin-1 and in To...  Read full blog post.

MyD88 Antibodies for IL Signaling and Immunity Research
The myeloid differentiation protein MyD88 (myeloid differentiation primary response protein) was originally identified and characterized as a primary upregulated response gene in interleukin-6 mediated myeloid differentiation. Now, MyD88 is known to b...  Read full blog post.

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Bioinformatics

Gene Symbol MYD88
Entrez
Uniprot