RNF36 Antibody

Images

 
Western Blot: RNF36 Antibody [NB100-1207] - (0.5ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: RNF36 Antibody [NB100-1207] - Staining of paraffin embedded Human Cerebellum at dilution of 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RNF36 Antibody [NB100-1207] - (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

RNF36 Antibody Summary

Immunogen
Peptide with sequence C-GENKEPLHILHPQ corresponding to C-Terminus according to NP_892030.3, NP_542783.2.
Epitope
C-GENKEPLHILHPQ
Specificity
This antibody is expected to recognize both reported isoforms (NP_892030.3; NP_542783.2).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TRIM69
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 2 ug/ml
  • Immunohistochemistry 2.5 - 3.8 ug/ml
  • Immunohistochemistry-Paraffin 2.5 - 3.8 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 55 kDa band observed in mouse testis lysates (calculated MW of 57.3 kDa band according to mouse NP_536771.2). IHC-P: Human testis shows speckled staining in nuclei of spermatocytes and spermitids.
Publications
Read Publication using NB100-1207.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RNF36 Antibody

  • RFP-like domain-containing protein trimless
  • RING finger protein 36HSD34
  • RNF36
  • Trif
  • TRIMLESS
  • tripartite motif containing 69
  • tripartite motif protein 69
  • tripartite motif-containing 69
  • tripartite motif-containing protein 69

Background

The gene encoding this protein is specifically expressed in germ cells at round spermatid stages during spermatogenesis. The protein encoded by this gene contains an N-terminal RING finger motif, a B-box, and a C-terminal B-30.2-like domain. When overexpressed in COS-7 and HEK-293 cells, this protein was shown to induce cell apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC, IHC-P, PEP-ELISA

Publications for RNF36 Antibody (NB100-1207)(1)

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TRIM69
Entrez
Uniprot
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