TRAF6 Inhibitor Peptide Set [NBP2-26506] - The TRAF6 inhibitory peptide inhibits osteoclast ifferentiation: RAW264.7 cells were plated on 24-well plates and stimulated with RANKL and M-CSF and incubated in the presence ...read more
Functions as a TRAF6 decoy by binding to the T6DP motif of RANK, thereby preventing binding of RANK to TRAF6.
The TRAF6 inhibitory peptide also contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable. The control peptide consists of only the PTD sequence.
Preparation of 5 mM Stock Solutions
PBS* is added directly to the vials to prepare the stock solutions. Note: Bring the solution to room temperature and quick spin the tubes before opening the caps.
TRAF6 Inhibitor Peptide: 1 mg of DRQIKIWFQNRRMKWKKRKIPTEDEY
Add 57.7 ul of PBS* to the vial to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing.
Control Peptide: 1 mg of DRQIKIWFQNRRMKWKK
Add 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at 20C or -80C. Avoid repeated freeze thawing.
*Recipe for 1X PBS:
1. Dissolve the following in 800ml distilled H2O.
- 8g of NaCl
- 0.2g of KCl
- 1.44g of Na2HPO4
- 0.24g of KH2PO4
2. Adjust pH to 7.5 with HCl.
3. Adjust volume to 1L with additional distilled H2O.
4. Sterilize by autoclaving
TRAF6 Inhibitor peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKKRKIPTEDEY (TRAF6 binding sequence: RKIPTEDEY). Molecular weight: 3494.
Antennapedia Control peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKK. Molecular weight: 2361.
In vitro assay
Inhibition of RANKL mediated osteoclast differentiation.
Researchers can study the effect of TRAF6 inhibitor peptide using a variety of methods, such as EMSA, NF-kB/p65 ELISA,IkBa phosphorylation ELISA, osteoclast differentiation assay. The Osteoclast formation assay protocol is a guideline which may need to be optimized for different cell types. Use in In vitro assay and blocking / neutralizing reported in scientific literature (PMID 24829345)
Read Publications using NBP2-26506 in the following applications:
Solubilize the peptides prior to use by making 5 mM PBS* stock solutions (please see Preparation of 5 mM Stock Solutions under Preparation Method).
Please contact technical support for detailed reconstitution instructions.
Alternate Names for TRAF-6 Inhibitor Peptide Set
E3 ubiquitin-protein ligase TRAF6
Interleukin-1 signal transducer
RING finger protein 85
TNF receptor-associated factor 6
TRAF6 is an adapter protein that links RANK with downstream signaling molecules, and plays a key role in RANKL mediated osteoclast differentiation. RANK contains a novel TRAF6-binding motif (T6DP) through which it binds to TRAF6 upon activation by RANKL. The TRAF6 inhibitory peptide contains a sequence from the T6DP motif of mouse RANK. The peptide binds to RANK, blocking TRAF6 and RANK interaction and thereby inhibiting osteoclast differentiation.
The TRAF6 inhibitory peptide also contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable.The control peptide consists of only the PTD sequence
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.