TRAF-6 Inhibitor Peptide Set


TRAF6 Inhibitor Peptide Set [NBP2-26506] - The TRAF6 inhibitory peptide inhibits osteoclast ifferentiation: RAW264.7 cells were plated on 24-well plates and stimulated with RANKL and M-CSF and incubated in the presence more

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Reactivity Hu, Mu, RtSpecies Glossary

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TRAF-6 Inhibitor Peptide Set Summary

Functions as a TRAF6 decoy by binding to the T6DP motif of RANK, thereby preventing binding of RANK to TRAF6.
The TRAF6 inhibitory peptide also contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable. The control peptide consists of only the PTD sequence.
Preparation of 5 mM Stock Solutions
PBS* is added directly to the vials to prepare the stock solutions. Note: Bring the solution to room temperature and quick spin the tubes before opening the caps.

Add 57.7 ul of PBS* to the vial to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing.

Control Peptide: 1 mg of DRQIKIWFQNRRMKWKK
Add 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at 20C or -80C. Avoid repeated freeze thawing.

*Recipe for 1X PBS:
1. Dissolve the following in 800ml distilled H2O.
- 8g of NaCl
- 0.2g of KCl
- 1.44g of Na2HPO4
- 0.24g of KH2PO4
2. Adjust pH to 7.5 with HCl.
3. Adjust volume to 1L with additional distilled H2O.
4. Sterilize by autoclaving
TRAF6 Inhibitor peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKKRKIPTEDEY (TRAF6 binding sequence: RKIPTEDEY). Molecular weight: 3494. Antennapedia Control peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKK. Molecular weight: 2361.


Application Notes
Inhibition of RANKL mediated osteoclast differentiation.

Researchers can study the effect of TRAF6 inhibitor peptide using a variety of methods, such as EMSA, NF-kB/p65 ELISA,IkBa phosphorylation ELISA, osteoclast differentiation assay. The Osteoclast formation assay protocol is a guideline which may need to be optimized for different cell types. Use in In vitro assay and blocking / neutralizing reported in scientific literature (PMID 24829345)
Read Publications using
NBP2-26506 in the following applications:

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Form: White Solid

Solubilize the peptides prior to use by making 5 mM PBS* stock solutions (please see Preparation of 5 mM Stock Solutions under Preparation Method).
Reconstitution Instructions
Please contact technical support for detailed reconstitution instructions.

Alternate Names for TRAF-6 Inhibitor Peptide Set

  • E3 ubiquitin-protein ligase TRAF6
  • EC 6.3.2.-
  • Interleukin-1 signal transducer
  • RING finger protein 85
  • RNF85MGC:3310
  • TNF receptor-associated factor 6
  • TRAF6
  • TRAF-6


TRAF6 is an adapter protein that links RANK with downstream signaling molecules, and plays a key role in RANKL mediated osteoclast differentiation. RANK contains a novel TRAF6-binding motif (T6DP) through which it binds to TRAF6 upon activation by RANKL. The TRAF6 inhibitory peptide contains a sequence from the T6DP motif of mouse RANK. The peptide binds to RANK, blocking TRAF6 and RANK interaction and thereby inhibiting osteoclast differentiation.

The TRAF6 inhibitory peptide also contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable.The control peptide consists of only the PTD sequence


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

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Publications for TRAF-6 Inhibitor (NBP2-26506)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: B/N, Func, In vitro.

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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NBP2-26506 Applications Species
Biswas S, Zimman A, Gao D et al. TLR2 Plays a Key Role in Platelet Hyperreactivity and Accelerated Thrombosis Associated with Hyperlipidemia Circ. Res. 2017 Aug 03 [PMID: 28775078] (B/N, Mouse) B/N Mouse
Wu DJ, Gu R, Sarin R et al. Autophagy-linked FYVE containing protein WDFY3 interacts with TRAF6 and modulates RANKL-induced osteoclastogenesis. J. Autoimmun. Jun 18 2016 [PMID: 27330028] (Mouse) Mouse
Hou W, Jin YH, Kang HS, Kim BS. IL-6 and IL-17 synergistically promote viral persistence by inhibiting cellular apoptosis and cytotoxic T cell function. J. Virol. 2014 May 14 [PMID: 24829345] (In vitro, B/N, Mouse)

Mouse bone marrow cells, Fig 7C. Cells were treated with 100 uM TRAF6 inhibitory or control peptides prior to cytokine stimulation.
In vitro, B/N Mouse
Brown GT, Narayanan P, Li W et al. Lipopolysaccharide Stimulates Platelets through an IL-1beta Autocrine Loop. J Immunol 2013 Nov 15 [PMID: 24081990] (Func, Human) Func Human
Brown GT, McIntyre TM. Lipopolysaccharide signaling without a nucleus: kinase cascades stimulate platelet shedding of proinflammatory IL-1B-rich microparticles. J Immunol. 2011 May 1 [PMID: 21430222]
Majewski Pawel M, Thurston Robert D, Ramalingam Rajalakshmy et al. Cooperative role of NF-{kappa}B and poly(ADP-ribose) polymerase 1 (PARP-1) in the TNF-induced inhibition of PHEX expression in osteoblasts. J Biol Chem. 2010 Nov 5 [PMID: 20817730]
Krupinski Elizabeth A. Virtual slide telepathology workstation of the future: lessons learned from teleradiology. Hum Pathol. 2009 Aug [PMID: 19552939] (Human) Human
Zhang Y, Saccani S, Shin H, Nikolajczyk BS. Dynamic protein associations define two phases of IL-1beta transcriptional activation. J Immunol. 2008 Jul 1 [PMID: 18566416]
Poblenz AT, Jacoby JJ, Singh S, Darnay BG. Inhibition of RANKL-mediated osteoclast differentiation by selective TRAF6 decoy peptides. Biochem Biophys Res Commun. 2007 Aug 3 [PMID: 17544369]
Takada Y, Aggarwal BB. Evidence that genetic deletion of the TNF receptor p60 or p80 in macrophages modulates RANKL-induced signaling. Blood. 2004 Dec 15 [PMID: 15315973]
Show All 12 Publications.

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Gene Symbol TRAF6