IRAK1 Antibody

Images

 
Western Blot: IRAK1 Antibody [NBP1-77068] - Analysis of IRAK in THP-1 (THP) and HeLa (HL) whole cell lysates using NBP1-77068 at 1:2000 dilution.
Immunocytochemistry/ Immunofluorescence: IRAK1 Antibody [NBP1-77068] - HeLa cells.
Immunocytochemistry/ Immunofluorescence: IRAK1 Antibody [NBP1-77068] - HeLa cells with IRAK antibody at 10 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

IRAK1 Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK. The immunogen is located within the last 50 amino acids of IRAK.
Specificity
At least three isoforms of IRAK are known to exist; this antibody will detect all three isoforms. IRAK antibody is predicted to not cross-react with other members of the IRAK protein family.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IRAK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
IRAK antibody can be used for Western blot at 1:1000 dilution and for immunoprecipitation with 2 to 4 ug per sample. An 80 kDa band should be detected in the lysate from non-activated cells. It has no cross response to IRAK2.
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
IRAK1 Peptide (NBP1-77068PEP)
Publications
Read Publication using NBP1-77068.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IRAK1 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • Il1rak
  • interleukin-1 receptor-associated kinase 1
  • IRAK1
  • IRAK-1
  • IRAKPelle homolog
  • Pelle
  • Plpk

Background

Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-kappaB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IRAK1 Antibody (NBP1-77068)(1)

We have publications tested in 1 confirmed species: Rat.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IRAK1
Entrez
Uniprot