IRAK1 Antibody


Western Blot: IRAK1 Antibody [NBP1-77068] - Species Activity in Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: IRAK NBP1-77068 (1 ug/mL,), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat more
Immunocytochemistry/ Immunofluorescence: IRAK1 Antibody [NBP1-77068] - Immunofluorescence Validation of IRAK in Human HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa Cells labeling IRAK with more
Western Blot: IRAK1 Antibody [NBP1-77068] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: IRAK NBP1-77068 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat more
Independent Antibodies: Western Blot: IRAK1 Antibody [NBP1-77068] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: IRAK more
Expression of Tagged Proteins: Western Blot: IRAK1 Antibody [NBP1-77068] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IRAK recombinant protein per lane.Antibodies: IRAK NBP1-77068 more
Immunocytochemistry/ Immunofluorescence: IRAK1 Antibody [NBP1-77068] - Immunocytochemistry Validation of IRAK in Human HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-IRAK antibody (NBP1-77068) at 10 ug more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
1 mg/ml

Order Details

IRAK1 Antibody Summary

Antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK. The immunogen is located within the last 50 amino acids of IRAK. Amino Acid Squence: DRQGPEESDEFQS
At least three isoforms of IRAK1 are known to exist; this antibody will detect all three isoforms. IRAK1 antibody is predicted to not cross-react with other members of the IRAK protein family.
Peptide affinity purified
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  • Western Blot 1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 10-20 ug/ml
  • Immunoprecipitation 2-4 ug/ml
Application Notes
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence and Immunocytochemistry in human samples. All other applications and species not yet tested. An 80 kDa band should be detected in the lysate from non-activated cells.
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
IRAK1 Peptide (NBP1-77068PEP)
Read Publication using NBP1-77068.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (85%)

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1 mg/ml
Peptide affinity purified

Alternate Names for IRAK1 Antibody

  • EC 2.7.11
  • EC
  • Il1rak
  • interleukin-1 receptor-associated kinase 1
  • IRAK1
  • IRAK-1
  • IRAKPelle homolog
  • Pelle
  • Plpk


Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-kappaB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IRAK1 Antibody (NBP1-77068)(1)

We have publications tested in 1 confirmed species: Rat.

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WB Video Protocol
ICC/IF Video Protocol

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Gene Symbol IRAK1