IRAK1 Antibody

Western Blot: IRAK1 Antibody [NBP1-77068] - Analysis of IRAK in THP-1 (THP) and HeLa (HL) whole cell lysates using NBP1-77068 at 1:2000 dilution.

Immunocytochemistry/Immunofluorescence: IRAK1 Antibody [NBP1-77068] - HeLa cells.

Immunocytochemistry/Immunofluorescence: IRAK1 Antibody [NBP1-77068] - HeLa cells with IRAK antibody at 10 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

IRAK1 Antibody Summary

• Immunogen: Antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK. The immunogen is located within the last 50 amino acids of IRAK.
• Specificity: At least three isoforms of IRAK are known to exist; this antibody will detect all three isoforms. IRAK antibody is predicted to not cross-react with other members of the IRAK protein family.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IRAK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Immunoprecipitation 2-4 ug
• Application Notes: IRAK antibody can be used for Western blot at 1:1000 dilution and for immunoprecipitation with 2 to 4 ug per sample. An 80 kDa band should be detected in the lysate from non-activated cells. It has no cross response to IRAK2.
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  IRAK1 Peptide (NBP1-77068PEP)

Reactivity Notes

Predicted species reactivity based on immunogen sequence: Bovine: (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IRAK1 Antibody

• EC 2.7.11
• EC 2.7.11.1
• Il1rak
• interleukin-1 receptor-associated kinase 1
• IRAK1
• IRAK-1
• IRAKPelle homolog
• Pelle
• Plpk

Background

Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-kappaB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRAK1 Antibody (NBP1-77068)(1)

Publication using NBP1-77068

• Chen XX, Tang L, Fu YM et al. Paralemmin-3 contributes to lipopolysaccharide-induced inflammatory response and is involved in lipopolysaccharide-Toll-like receptor-4 signaling in alveolar macrophages. Int. J. Mol. Med. 2017 Sep 28 [PMID: 29039447] (Rat)

Bioinformatics

• Gene Symbol: IRAK1
• Entrez:
  • 3654(Human)
  • 16179(Mouse)
  • 363520(Rat)
• Uniprot:
  • P51617(Human)