Western Blot: IRAK1 Antibody [NBP1-77068] - Species Activity in Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: IRAK NBP1-77068 (1 ug/mL,), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat ...read more
Immunocytochemistry/ Immunofluorescence: IRAK1 Antibody [NBP1-77068] - Immunofluorescence Validation of IRAK in Human HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa Cells labeling IRAK with ...read more
Western Blot: IRAK1 Antibody [NBP1-77068] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: IRAK NBP1-77068 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat ...read more
Independent Antibodies: Western Blot: IRAK1 Antibody [NBP1-77068] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: IRAK ...read more
Expression of Tagged Proteins: Western Blot: IRAK1 Antibody [NBP1-77068] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IRAK recombinant protein per lane.Antibodies: IRAK NBP1-77068 ...read more
Immunocytochemistry/ Immunofluorescence: IRAK1 Antibody [NBP1-77068] - Immunocytochemistry Validation of IRAK in Human HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-IRAK antibody (NBP1-77068) at 10 ug ...read more
Antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK. The immunogen is located within the last 50 amino acids of IRAK.
Human IRAK has 4 isoforms, including isoform 1 (712aa, 77kD), isoform 2 (682aa, 73kD), isoform 3 (693aa, 75kD), and isoform 4 (633aa, 68kD). Mouse IRAK has 2 isoforms, including isoform 1 (710aa, 77kD) and isoform 2 (712aa, 78kD). Rat IRAK also has one isoform (710aa, 78kD). 1007 can detect all human isoforms, mouse and rat IRAK. IRAK antibody is predicted to not cross-react with other members of the IRAK protein family.
Peptide affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence and Immunocytochemistry in human samples. All other applications and species not yet tested. An 80 kDa band should be detected in the lysate from non-activated cells.
77 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Predicted species reactivity based on immunogen sequence: Bovine: (85%)
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Peptide affinity purified
Alternate Names for IRAK1 Antibody
interleukin-1 receptor-associated kinase 1
Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-kappaB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.