IRAK1 Antibody

Western Blot: IRAK1 Antibody [NBP1-77068] - Species Activity in Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: IRAK NBP1-77068 (1 ug/mL,), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/Immunofluorescence: IRAK1 Antibody [NBP1-77068] - Immunofluorescence Validation of IRAK in Human HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa Cells labeling IRAK with NBP1-77068 at 20 ug g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Western Blot: IRAK1 Antibody [NBP1-77068] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: IRAK NBP1-77068 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: IRAK1 Antibody [NBP1-77068] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: IRAK NBP1-77068 (1 ug/mL), IRAK from Company B (2 ug/mL), beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: IRAK1 Antibody [NBP1-77068] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IRAK recombinant protein per lane.Antibodies: IRAK NBP1-77068 (1: 1ug/mL, 2: 2 ug/mL and 3: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/Immunofluorescence: IRAK1 Antibody [NBP1-77068] - Immunocytochemistry Validation of IRAK in Human HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-IRAK antibody (NBP1-77068) at 10 ug g/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

IRAK1 Antibody Summary

• Immunogen: Antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK. The immunogen is located within the last 50 amino acids of IRAK. Amino Acid Squence: DRQGPEESDEFQS
• Specificity: At least three isoforms of IRAK1 are known to exist; this antibody will detect all three isoforms. IRAK1 antibody is predicted to not cross-react with other members of the IRAK protein family.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IRAK1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 10-20 ug/ml
  • Immunoprecipitation 2-4 ug/ml
• Application Notes: Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence and Immunocytochemistry in human samples. All other applications and species not yet tested. An 80 kDa band should be detected in the lysate from non-activated cells.
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  IRAK1 Peptide (NBP1-77068PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for IRAK1 Antibody

• EC 2.7.11
• EC 2.7.11.1
• Il1rak
• interleukin-1 receptor-associated kinase 1
• IRAK1
• IRAK-1
• IRAKPelle homolog
• Pelle
• Plpk

Background

Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-kappaB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRAK1 Antibody (NBP1-77068)(1)

Publication using NBP1-77068

• Chen XX, Tang L, Fu YM et al. Paralemmin-3 contributes to lipopolysaccharide-induced inflammatory response and is involved in lipopolysaccharide-Toll-like receptor-4 signaling in alveolar macrophages. Int. J. Mol. Med. 2017 Sep 28 [PMID: 29039447] (Rat)

Bioinformatics

• Gene Symbol: IRAK1
• Uniprot:
  • P51617(Human)